Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand.

Abstract:

:Due to their multipotentiality and immunomodulation, human mesenchymal stem cells (hMSCs) are widely studied for the treatment of degenerative and inflammatory diseases. Transplantation of hMSCs to damaged tissue is a promising approach for tissue regeneration. However, the physiological mechanisms and regulatory processes of MSC trafficking to injured tissue are largely unexplored. Here, we evaluated the gene expression profile and migratory potential of hMSCs upon stimulation with the TLR4 ligand lipopolysaccharide (LPS). Using RNA sequencing, we identified unique induction patterns of interferon stimulated genes, cytokines and chemokines involved in chemotaxis and homing. The -950 to +50 bp regions of many of these LPS-responsive genes were enriched with putative binding motifs for the transcription factors (TFs) interferon regulatory factor (IRF1) and nuclear factor kappa B (NF-κB1, REL), which were also induced by LPS along with other TFs. Chromatin immunoprecipitation assays showed that IRF1 bound within their target genes promoter region. In addition, IRF1 attenuation significantly down-regulated interferon stimulated genes as well as key cytokines. Furthermore, using pharmacological inhibitors, we showed that the NF-κB and phosphatidylinositol 3-kinase (PI3K) pathways regulate the migratory and cytokines/chemokines response to LPS. These unprecedented data suggest that IRF1 and NF-κB orchestrate the TLR4-primed immunomodulatory response of hMSCs and that this response also involves the PI3K pathway.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Kim SH,Das A,Chai JC,Binas B,Choi MR,Park KS,Lee YS,Jung KH,Chai YG

doi

10.1038/srep30311

subject

Has Abstract

pub_date

2016-07-22 00:00:00

pages

30311

issn

2045-2322

pii

srep30311

journal_volume

6

pub_type

杂志文章
  • Invisibility Cloaking Scheme by Evanescent Fields Distortion on Composite Plasmonic Waveguides with Si Nano-Spacer.

    abstract::A new, composite plasmonic waveguide based electromagnetic cloaking scheme is proposed with Si nano-spacer. Here we show, that the scattering fields of an object located on the cloak do not interact with the evanescent field, resulting in object's invisibility. Finite difference time domain (FDTD) numerical calculatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10578-6

    authors: Galutin Y,Falek E,Karabchevsky A

    更新日期:2017-09-21 00:00:00

  • The sequence at Spike S1/S2 site enables cleavage by furin and phospho-regulation in SARS-CoV2 but not in SARS-CoV1 or MERS-CoV.

    abstract::The Spike protein of the novel coronavirus SARS-CoV2 contains an insertion 680SPRRAR↓SV687 forming a cleavage motif RxxR for furin-like enzymes at the boundary of S1/S2 subunits. Cleavage at S1/S2 is important for efficient viral entry into target cells. The insertion is absent in other CoV-s of the same clade, includ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74101-0

    authors: Örd M,Faustova I,Loog M

    更新日期:2020-10-09 00:00:00

  • Density-controlled quantum Hall ferromagnetic transition in a two-dimensional hole system.

    abstract::Quantum Hall ferromagnetic transitions are typically achieved by increasing the Zeeman energy through in-situ sample rotation, while transitions in systems with pseudo-spin indices can be induced by gate control. We report here a gate-controlled quantum Hall ferromagnetic transition between two real spin states in a c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02757-2

    authors: Lu TM,Tracy LA,Laroche D,Huang SH,Chuang Y,Su YH,Li JY,Liu CW

    更新日期:2017-06-01 00:00:00

  • Immobilization of Polyoxometalate in the Metal-Organic Framework rht-MOF-1: Towards a Highly Effective Heterogeneous Catalyst and Dye Scavenger.

    abstract::A series of three remarkable complexes [PMo12O40]@[Cu6O(TZI)3(H2O)9]4·OH·31H2O (H3TZI = 5-tetrazolylisophthalic acid; denoted as HLJU-1, HLJU = Heilongjiang University), [SiMo12O40]@[Cu6O(TZI)3(H2O)9]4·32H2O (denoted as HLJU-2), and [PW12O40]@[Cu6O(TZI)3(H2O)6]4·OH·31H2O (denoted as HLJU-3) have been isolated by using...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep25595

    authors: Sun JW,Yan PF,An GH,Sha JQ,Li GM,Yang GY

    更新日期:2016-05-09 00:00:00

  • Dynamic features of water molecules in superconcentrated aqueous electrolytes.

    abstract::An existence of ions dissolved in water has significant effects on bulk properties of water. Superconcentrated conditions have been recently proposed to provide a new concept of lithium ion batteries in order to overcome limitations for practical applications. In those conditions, water would undergo significant chang...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27706-5

    authors: Han S

    更新日期:2018-06-19 00:00:00

  • Room temperature electrical spin injection into GaAs by an oxide spin injector.

    abstract::Spin injection, manipulation and detection are the integral parts of spintronics devices and have attracted tremendous attention in the last decade. It is necessary to judiciously choose the right combination of materials to have compatibility with the existing semiconductor technology. Conventional metallic magnets w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05588

    authors: Bhat SG,Kumar PS

    更新日期:2014-07-07 00:00:00

  • A novel laser-collider used to produce monoenergetic 13.3 MeV (7)Li (d, n) neutrons.

    abstract::Neutron energy is directly correlated with the energy of the incident ions in experiments involving laser-driven nuclear reactions. Using high-energy incident ions reduces the energy concentration of the generated neutrons. A novel "laser-collider" method was used at the Shenguang II laser facility to produce monoener...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27363

    authors: Zhao JR,Zhang XP,Yuan DW,Li YT,Li DZ,Rhee YJ,Zhang Z,Li F,Zhu BJ,Li YF,Han B,Liu C,Ma Y,Li YF,Tao MZ,Li MH,Guo X,Huang XG,Fu SZ,Zhu JQ,Zhao G,Chen LM,Fu CB,Zhang J

    更新日期:2016-06-02 00:00:00

  • In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia-Telangiectasia patients.

    abstract::Short term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia-Telangiectasia (A-T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are u...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77352-z

    authors: Pozzi E,Giorgio E,Mancini C,Lo Buono N,Augeri S,Ferrero M,Di Gregorio E,Riberi E,Vinciguerra M,Nanetti L,Bianchi FT,Sassi MP,Costanzo V,Mariotti C,Funaro A,Cavalieri S,Brusco A

    更新日期:2020-11-19 00:00:00

  • A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification.

    abstract::Hepcidin, a cysteine-rich peptide hormone, secreted mainly by the liver, plays a central role in iron metabolism regulation. Emerging evidence suggests that disordered iron metabolism is a risk factor for various types of diseases including cancers. However, it remains challenging to apply current mass spectrometry (M...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43756-9

    authors: Moghieb A,Tesfay L,Nie S,Gritsenko M,Fillmore TL,Jacobs JM,Smith RD,Torti FM,Torti SV,Shi T,Ansong C

    更新日期:2019-05-13 00:00:00

  • Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK/p38 pathways in human acute promyelocytic leukemia cells.

    abstract::Ruthenium(II) complexes with 6-methyl-2-thiouracil cis-[Ru(6m2tu)2(PPh3)2] (1) and [Ru(6m2tu)2(dppb)] (2) (where PPh3 = triphenylphosphine; dppb = 1,4-bis(diphenylphosphino)butane; and 6m2tu = 6-methyl-2-thiouracil) are potent cytotoxic agents and able to bind DNA. The aim of this study was to evaluate in vitro cellul...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47914-x

    authors: Bomfim LM,de Araujo FA,Dias RB,Sales CBS,Rocha CAG,Correa RS,Soares MBP,Batista AA,Bezerra DP

    更新日期:2019-08-07 00:00:00

  • Achieving waste to energy through sewage sludge gasification using hot slags: syngas production.

    abstract::To relieve the environmental issues of sewage sludge (SS) disposal and greenhouse gas (GHG) emission in China, we proposed an integrated method for the first time to simultaneously deal with these two problems. The hot slags below 920 °C could act as a good heat carrier for sludge gasification and the increasing CO2 c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep11436

    authors: Sun Y,Nakano J,Liu L,Wang X,Zhang Z

    更新日期:2015-06-15 00:00:00

  • A High-Concentrate Diet Induced Milk Fat Decline via Glucagon-Mediated Activation of AMP-Activated Protein Kinase in Dairy Cows.

    abstract::Dairy cows are often fed a high-concentrate (HC) diet to meet lactation demands; however, long-term concentrate feeding is unhealthy and decreases milk fat. Therefore, we investigated the effects of liver lipid metabolism on milk fat synthesis. Ten lactating Holstein cows were assigned randomly into HC and LC (low-con...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44217

    authors: Li L,Cao Y,Xie Z,Zhang Y

    更新日期:2017-03-13 00:00:00

  • High performance C/S composite cathodes with conventional carbonate-based electrolytes in Li-S battery.

    abstract::High stable C/S composites are fabricated by a novel high-temperature sulfur infusion into micro-mesoporous carbon method following with solvent cleaning treatment. The C/S composite cathodes show high Coulombic efficiency, long cycling stability and good rate capability in the electrolyte of 1.0 M LiPF6 + EC/DEC (1:1...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04842

    authors: Zheng S,Han P,Han Z,Zhang H,Tang Z,Yang J

    更新日期:2014-04-29 00:00:00

  • Searchers adjust their eye-movement dynamics to target characteristics in natural scenes.

    abstract::When searching a target in a natural scene, it has been shown that both the target's visual properties and similarity to the background influence whether and how fast humans are able to find it. So far, it was unclear whether searchers adjust the dynamics of their eye movements (e.g., fixation durations, saccade ampli...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37548-w

    authors: Rothkegel LOM,Schütt HH,Trukenbrod HA,Wichmann FA,Engbert R

    更新日期:2019-02-07 00:00:00

  • Iron Pyrite/Titanium Dioxide Photoanode for Extended Near Infrared Light Harvesting in a Photoelectrochemical Cell.

    abstract::The design of active and stable semiconducting composites with enhanced photoresponse from visible light to near infrared (NIR) is a key to improve solar energy harvesting for photolysis of water in photoelectrochemical cell. In this study, we prepared earth abundant semiconducting composites consisting of iron pyrite...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20397

    authors: Wang DY,Li CH,Li SS,Kuo TR,Tsai CM,Chen TR,Wang YC,Chen CW,Chen CC

    更新日期:2016-02-08 00:00:00

  • A novel immunohistochemical score to predict early mortality in acute myeloid leukemia patients based on indoleamine 2,3 dioxygenase expression.

    abstract::Indoleamine 2,3 dioxygenase-1 (IDO-1) is an enzyme in the kynurenine pathway which augments tumor-induced immune tolerance. Previous studies in childhood acute myeloid leukemia (AML) have shown a negative correlation of IDO-1 mRNA expression with outcomes. The aim of our study was to develop a practical and objective ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12940-0

    authors: Mangaonkar A,Mondal AK,Fulzule S,Pundkar C,Park EJ,Jillella A,Kota V,Xu H,Savage NM,Shi H,Munn D,Kolhe R

    更新日期:2017-10-16 00:00:00

  • RNA polymerase II subunit D is essential for zebrafish development.

    abstract::DNA-directed RNA polymerase II (pol II) is composed of ten core and two dissociable subunits. The dissociable subcomplex is a heterodimer of Rpb4/Polr2d and Rpb7/Polr2g, which are encoded by RPB4/polr2d and RPB7/polr2g genes, respectively. Functional studies of Rpb4/Polr2d in yeast have revealed that Rpb4 plays a role...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70110-1

    authors: Maeta M,Kataoka M,Nishiya Y,Ogino K,Kashima M,Hirata H

    更新日期:2020-08-06 00:00:00

  • Molecular packing and electronic processes in amorphous-like polymer bulk heterojunction solar cells with fullerene intercalation.

    abstract::The interpenetrating morphology formed by the electron donor and acceptor materials is critical for the performance of polymer:fullerene bulk heterojunction (BHJ) photovoltaic (PV) cells. In this work we carried out a systematic investigation on a high PV efficiency (>6%) BHJ system consisting of a newly developed 5,6...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05211

    authors: Xiao T,Xu H,Grancini G,Mai J,Petrozza A,Jeng US,Wang Y,Xin X,Lu Y,Choon NS,Xiao H,Ong BS,Lu X,Zhao N

    更新日期:2014-06-09 00:00:00

  • Secrets of virtuoso: neuromuscular attributes of motor virtuosity in expert musicians.

    abstract::Musical performance requires extremely fast and dexterous limb movements. The underlying biological mechanisms have been an object of interest among scientists and non-scientists for centuries. Numerous studies of musicians and non-musicians have demonstrated that neuroplastic adaptations through early and deliberate ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15750

    authors: Furuya S,Oku T,Miyazaki F,Kinoshita H

    更新日期:2015-10-27 00:00:00

  • Non-invasive imaging of engineered human tumors in the living chicken embryo.

    abstract::The growing interest in engineered tumor models prompted us to devise a method for the non-invasive assessment of such models. Here, we report on bioluminescence imaging (BLI) for the assessment of engineered tumor models in the fertilized chicken egg, i.e, chick chorioallantoic membrane (CAM) assay. One prostate canc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04572-1

    authors: Jefferies B,Lenze F,Sathe A,Truong N,Anton M,von Eisenhart-Rothe R,Nawroth R,Mayer-Kuckuk P

    更新日期:2017-07-10 00:00:00

  • Characterization of biological variation of peripheral blood immune cytome in an Indian cohort.

    abstract::Immune parameters show characteristic normal baseline levels and variance in the population. We characterised the degree of inter-individual and within-individual variation over one-year time period in 33 immune cell subsets by flow cytometry in peripheral blood mononuclear cells from 43 healthy young adult volunteers...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51294-7

    authors: Kanodia P,Kaur G,Coshic P,Chatterjee K,Neeman T,George A,Rath S,Bal V,Prabhu SB

    更新日期:2019-10-14 00:00:00

  • A selective autophagy cargo receptor NBR1 modulates abscisic acid signalling in Arabidopsis thaliana.

    abstract::The plant selective autophagy cargo receptor neighbour of breast cancer 1 gene (NBR1) has been scarcely studied in the context of abiotic stress. We wanted to expand this knowledge by using Arabidopsis thaliana lines with constitutive ectopic overexpression of the AtNBR1 gene (OX lines) and the AtNBR1 Knock-Out (KO li...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64765-z

    authors: Tarnowski L,Rodriguez MC,Brzywczy J,Piecho-Kabacik M,Krčkova Z,Martinec J,Wawrzynska A,Sirko A

    更新日期:2020-05-08 00:00:00

  • Broad and Efficient Control of Klebsiella Pathogens by Peptidoglycan-Degrading and Pore-Forming Bacteriocins Klebicins.

    abstract::Gram-negative bacteria belonging to the genus Klebsiella are important nosocomial pathogens, readily acquiring resistance to all known antibiotics. Bacteriocins, non-antibiotic antibacterial proteins, have been earlier proposed as potential therapeutic agents for control of other Gram-negative species such as Escheric...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51969-1

    authors: Denkovskienė E,Paškevičius Š,Misiūnas A,Stočkūnaitė B,Starkevič U,Vitkauskienė A,Hahn-Löbmann S,Schulz S,Giritch A,Gleba Y,Ražanskienė A

    更新日期:2019-10-28 00:00:00

  • An experimental study of network effects on coordination in asymmetric games.

    abstract::Network structure has often proven to be important in understanding the decision behavior of individuals or agents in different interdependent situations. Computational studies predict that network structure has a crucial influence on behavior in iterated 2 by 2 asymmetric 'battle of the sexes' games. We test such beh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43260-0

    authors: Broere J,Buskens V,Stoof H,Sánchez A

    更新日期:2019-05-02 00:00:00

  • AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells.

    abstract::The mammalian AlkB homolog (ALKBH) family of proteins possess a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. A similar domain in the Escherichia coli AlkB protein catalyzes the oxidative demethylation of 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) in both DNA and RNA. AlkB homolog 3 (ALKBH3) was als...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep42271

    authors: Ueda Y,Ooshio I,Fusamae Y,Kitae K,Kawaguchi M,Jingushi K,Hase H,Harada K,Hirata K,Tsujikawa K

    更新日期:2017-02-13 00:00:00

  • Pro-arrhythmogenic effects of CACNA1C G1911R mutation in human ventricular tachycardia: insights from cardiac multi-scale models.

    abstract::Mutations in the CACNA1C gene are associated with ventricular tachycardia (VT). Although the CACNA1C mutations were well identified in patients with cardiac arrhythmias, mechanisms by which cardiac arrhythmias are generated in such genetic mutation conditions remain unclear. In this study, we identified a novel mechan...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31262

    authors: Bai J,Wang K,Li Q,Yuan Y,Zhang H

    更新日期:2016-08-09 00:00:00

  • Ecological niche modeling of Astragalus membranaceus var. mongholicus medicinal plants in Inner Mongolia, China.

    abstract::Radix Astragali is commonly used in traditional Chinese medicine, and its quality is closely related to ecological factors, such as climate and soil, in the production area. To provide high-quality Radix Astragali to Chinese and foreign markets, we used maximum entropy model and statistical analysis method, combined w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69391-3

    authors: Yang M,Li Z,Liu L,Bo A,Zhang C,Li M

    更新日期:2020-07-27 00:00:00

  • PD-L1 predicts poor prognosis for nasopharyngeal carcinoma irrespective of PD-1 and EBV-DNA load.

    abstract::Programmed death-1 (PD-1) is an immunosuppressive receptor functionally bound with programmed death-ligand 1 (PD-L1), which has been reported in various malignancies. However, only a few studies are available for the clinical significance of PD-1/PD-L1 in nasopharyngeal carcinoma (NPC). In this study, we aim to invest...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43627

    authors: Zhou Y,Shi D,Miao J,Wu H,Chen J,Zhou X,Hu D,Zhao C,Deng W,Xie C

    更新日期:2017-03-03 00:00:00

  • Author Correction: The role of directional interactions in the designability of generalized heteropolymers.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-22649-3

    authors: Cardelli C,Bianco V,Rovigatti L,Nerattini F,Tubiana L,Dellago C,Coluzza I

    更新日期:2018-03-12 00:00:00

  • Phenothiazine derivative-accelerated microbial extracellular electron transfer in bioelectrochemical system.

    abstract::In bioelectrochemical system (BES) the extracellular electron transfer (EET) from bacteria to anode electrode is recognized as a crucial step that governs the anodic reaction efficiency. Here, we report a novel approach to substantially enhance the microbial EET by immobilization of a small active phenothiazine deriva...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01616

    authors: Liu XW,Sun XF,Chen JJ,Huang YX,Xie JF,Li WW,Sheng GP,Zhang YY,Zhao F,Lu R,Yu HQ

    更新日期:2013-01-01 00:00:00