In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia-Telangiectasia patients.

Abstract:

:Short term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia-Telangiectasia (A-T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are unclear. We aimed at evaluating the effect of dexamethasone on the induction of alternative ATM transcripts (ATMdexa1). We showed that dexamethasone cannot induce an alternative ATM transcript in control and A-T lymphoblasts and primary fibroblasts, or in an ATM-knockout HeLa cell line. We also demonstrated that some of the reported readouts associated with ATMdexa1 are due to cellular artifacts and the direct induction of γH2AX by dexamethasone via DNA-PK. Finally, we suggest caution in interpreting dexamethasone effects in vitro for the results to be translated into a rational use of the drug in A-T patients.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Pozzi E,Giorgio E,Mancini C,Lo Buono N,Augeri S,Ferrero M,Di Gregorio E,Riberi E,Vinciguerra M,Nanetti L,Bianchi FT,Sassi MP,Costanzo V,Mariotti C,Funaro A,Cavalieri S,Brusco A

doi

10.1038/s41598-020-77352-z

subject

Has Abstract

pub_date

2020-11-19 00:00:00

pages

20182

issue

1

issn

2045-2322

pii

10.1038/s41598-020-77352-z

journal_volume

10

pub_type

杂志文章
  • Predictors of impending acute chest syndrome in patients with sickle cell anaemia.

    abstract::Acute chest syndrome (ACS) is a major complication of sickle cell anaemia (SCA) and a leading cause for hospital admissions and death. We aimed to study the spectrum of clinical and laboratory features of ACS and to assess the predisposing factors and predictors of severity. A retrospective case-control cohort was stu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59258-y

    authors: Alkindi S,Al-Busaidi I,Al-Salami B,Raniga S,Pathare A,Ballas SK

    更新日期:2020-02-12 00:00:00

  • Calcyphosine-like (CAPSL) is regulated in Multiple Symmetric Lipomatosis and is involved in Adipogenesis.

    abstract::Little is known on the causes and pathogenesis of the adipose tissue disorder (familial) Multiple Symmetric Lipomatosis (MSL). In a four-generation MSL-family, we performed whole exome sequencing (WES) in 3 affected individuals and 1 obligate carrier and identified Calcyphosine-like (CAPSL) as the most promising candi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44382-1

    authors: Lindner A,Marbach F,Tschernitz S,Ortner C,Berneburg M,Felthaus O,Prantl L,Kye MJ,Rappl G,Altmüller J,Thiele H,Schreml S,Schreml J

    更新日期:2019-06-11 00:00:00

  • Two-dimensional β-MnO₂ nanowire network with enhanced electrochemical capacitance.

    abstract::Conventional crystalline β-MnO2 usually exhibits poor electrochemical activities due to the narrow tunnels in its rutile-type structure. In this study, we synthesized a novel 2D β-MnO2 network with long-range order assembled by β-MnO2 nanowires and demonstrated that the novel 2D β-MnO2 network exhibits enhanced electr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02193

    authors: Wei C,Pang H,Zhang B,Lu Q,Liang S,Gao F

    更新日期:2013-01-01 00:00:00

  • A jumping shape memory alloy under heat.

    abstract::Shape memory alloys are typical temperature-sensitive metallic functional materials due to superelasticity and shape recovery characteristics. The conventional shape memory effect involves the formation and deformation of thermally induced martensite and its reverse transformation. The shape recovery process usually t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21754

    authors: Yang S,Omori T,Wang C,Liu Y,Nagasako M,Ruan J,Kainuma R,Ishida K,Liu X

    更新日期:2016-02-16 00:00:00

  • Novelty seeking is related to individual risk preference and brain activation associated with risk prediction during decision making.

    abstract::Novelty seeking (NS) is a personality trait reflecting excitement in response to novel stimuli. High NS is usually a predictor of risky behaviour such as drug abuse. However, the relationships between NS and risk-related cognitive processes, including individual risk preference and the brain activation associated with...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/srep10534

    authors: Wang Y,Liu Y,Yang L,Gu F,Li X,Zha R,Wei Z,Pei Y,Zhang P,Zhou Y,Zhang X

    更新日期:2015-06-11 00:00:00

  • Compact Shielding of Graphene Monolayer Leads to Extraordinary SERS-Active Substrate with Large-Area Uniformity and Long-Term Stability.

    abstract::Surface-enhanced Raman scattering (SERS) can significantly boost the inherently weak Raman scattering signal and provide detailed structural information and binding nature of the molecules on the surface. Despite the long history of this technology, SERS has yet to become a sophisticated analytical tool in practical a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17167

    authors: Liu X,Wang J,Wu Y,Fan T,Xu Y,Tang L,Ying Y

    更新日期:2015-11-30 00:00:00

  • A comparative study of sleep and diurnal patterns in house mouse (Mus musculus) and Spiny mouse (Acomys cahirinus).

    abstract::Most published sleep studies use three species: human, house mouse, or Norway rat. The degree to which data from these species captures variability in mammalian sleep remains unclear. To gain insight into mammalian sleep diversity, we examined sleep architecture in the spiny basal murid rodent Acomys cahirinus. First,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67859-w

    authors: Wang C,Guerriero LE,Huffman DM,Ajwad AA,Brooks TC,Sunderam S,Seifert AW,O'Hara BF

    更新日期:2020-07-02 00:00:00

  • Cystoid edema, neovascularization and inflammatory processes in the murine Norrin-deficient retina.

    abstract::Mutations in the Norrin (NDP) gene cause severe developmental blood vessel defects in the retina leading to congenital blindness. In the retina of Ndph-knockout mice only the superficial capillary network develops. Here, a detailed characterization of this mouse model at late stages of the disease using in vivo retina...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24476-y

    authors: Beck SC,Karlstetter M,Garcia Garrido M,Feng Y,Dannhausen K,Mühlfriedel R,Sothilingam V,Seebauer B,Berger W,Hammes HP,Seeliger MW,Langmann T

    更新日期:2018-04-13 00:00:00

  • MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a.

    abstract::Nonalcoholic fibrosing steatohepatitis is a uniform process throughout nonalcoholic fatty liver disease (NAFLD). MicroRNAs (miRNAs) have been suggested to modulate cellular processes in liver diseases. However, the functional role of miRNAs in nonalcoholic fibrosing steatohepatitis is largely unclear. In this study, w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16163

    authors: Du J,Niu X,Wang Y,Kong L,Wang R,Zhang Y,Zhao S,Nan Y

    更新日期:2015-11-05 00:00:00

  • Retinal involvement and ocular findings in COVID-19 pneumonia patients.

    abstract::Changes in immune and coagulation systems and possible viral spread through the blood-brain barrier have been described in SARS-CoV-2 infection. In this study, we evaluated the possible retinal involvement and ocular findings in severe COVID-19 pneumonia patients. A cross-sectional study was conducted on 46 patients a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74446-6

    authors: Pirraglia MP,Ceccarelli G,Cerini A,Visioli G,d'Ettorre G,Mastroianni CM,Pugliese F,Lambiase A,Gharbiya M

    更新日期:2020-10-15 00:00:00

  • Elucidating the Mechanism of Weissella-dependent Lifespan Extension in Caenorhabditis elegans.

    abstract::The mechanism whereby lactic acid bacteria extend the lifespan of Caenorhabditis elegans has previously been elucidated. However, the role of Weissella species has yet not been studied. We show that Weissella koreensis and Weissella cibaria significantly (p < 0.05) extend the lifespan of C. elegans compared with Esche...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17128

    authors: Lee J,Kwon G,Lim YH

    更新日期:2015-11-25 00:00:00

  • Surface and Bulk Carbide Transformations in High-Speed Steel.

    abstract::We have studied the transformation of carbides in AISI M42 high-speed steels in the temperature window used for forging. The annealing was found to result in the partial transformation of the large, metastable M2C carbides into small, more stable grains of M6C, with an associated change in the crystal orientation. In ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16202

    authors: Godec M,Večko Pirtovšek T,Šetina Batič B,McGuiness P,Burja J,Podgornik B

    更新日期:2015-11-05 00:00:00

  • Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor.

    abstract::Hydrogen recovery through solar-driven biomass conversion by photo-fermentative bacteria (PFB) has been regarded as a promising way for sustainable energy production. However, a considerable fraction of organic substrate was consumed for the growth of PFB as biocatalysts, furthermore, these PFB were continuously washe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16174

    authors: Xie GJ,Liu BF,Wang RQ,Ding J,Ren HY,Zhou X,Ren NQ

    更新日期:2015-11-05 00:00:00

  • Wild chimpanzees show group differences in selection of agricultural crops.

    abstract::The ability of wild animals to respond flexibly to anthropogenic environmental changes, including agriculture, is critical to survival in human-impacted habitats. Understanding use of human foods by wildlife can shed light on the acquisition of novel feeding habits and how animals respond to human-driven land-use chan...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05956

    authors: McLennan MR,Hockings KJ

    更新日期:2014-08-05 00:00:00

  • Circulating microRNAs correlated with the level of coronary artery calcification in symptomatic patients.

    abstract::The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with the severity of coronary artery calcification (CAC), and testify whether the selected miRNAs could reflect the obstructive coronary artery disease in symptomatic patients. Patients with chest pain and moderated risk for coronary a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16099

    authors: Liu W,Ling S,Sun W,Liu T,Li Y,Zhong G,Zhao D,Zhang P,Song J,Jin X,Xu Z,Song H,Li Q,Liu S,Chai M,Dai Q,He Y,Fan Z,Zhou YJ,Li Y

    更新日期:2015-11-05 00:00:00

  • Comparative studies of alignment, alignment-free and SVM based approaches for predicting the hosts of viruses based on viral sequences.

    abstract::Predicting the hosts of newly discovered viruses is important for pandemic surveillance of infectious diseases. We investigated the use of alignment-based and alignment-free methods and support vector machine using mononucleotide frequency and dinucleotide bias to predict the hosts of viruses, and applied these approa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28308-x

    authors: Li H,Sun F

    更新日期:2018-07-03 00:00:00

  • Iodine accumulation of the liver in patients treated with amiodarone can be unmasked using material decomposition from multiphase spectral-detector CT.

    abstract::Amiodarone accumulates in the liver, where it increases x-ray attenuation due to its iodine content. We evaluated liver attenuation in patients treated and not treated with amiodarone using true-non-contrast (TNC) and virtual-non-contrast (VNC) images acquired with spectral-detector-CT (SDCT). 142 patients, of which 2...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64002-7

    authors: Laukamp KR,Lennartz S,Hashmi A,Obmann M,Ho V,Große Hokamp N,Graner FP,Gilkeson R,Persigehl T,Gupta A,Ramaiya N

    更新日期:2020-04-24 00:00:00

  • Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations.

    abstract::The synaptic protein SHANK3 encodes a multidomain scaffold protein expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified de novo SHANK3 mutations in patients with autism spectrum disorders (ASD) and showed that SHANK3 represents one of the major genes for ASD. Here, we analyze...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36993-x

    authors: Gouder L,Vitrac A,Goubran-Botros H,Danckaert A,Tinevez JY,André-Leroux G,Atanasova E,Lemière N,Biton A,Leblond CS,Poulet A,Boland A,Deleuze JF,Benchoua A,Delorme R,Bourgeron T,Cloëz-Tayarani I

    更新日期:2019-01-14 00:00:00

  • Dislocation-twin boundary interactions induced nanocrystalline via SPD processing in bulk metals.

    abstract::This report investigated dislocation-twin boundary (TB) interactions that cause the TB to disappear and turn into a high-angle grain boundary (GB). The evolution of the microstructural characteristics of Hadfield steel was shown as a function of severe plastic deformation processing time. Sessile Frank partial disloca...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08981

    authors: Zhang F,Feng X,Yang Z,Kang J,Wang T

    更新日期:2015-03-11 00:00:00

  • Identification of ancestry proportions in admixed groups across the Americas using clinical pharmacogenomic SNP panels.

    abstract::We evaluated the performance of three PGx panels to estimate biogeographical ancestry: the DMET panel, and the VIP and Preemptive PGx panels described in the literature. Our analysis indicate that the three panels capture quite well the individual variation in admixture proportions observed in recently admixed populat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80389-9

    authors: Debortoli G,de Araujo GS,Fortes-Lima C,Parra EJ,Suarez-Kurtz G

    更新日期:2021-01-13 00:00:00

  • Dike intrusions during rifting episodes obey scaling relationships similar to earthquakes.

    abstract::As continental rifts evolve towards mid-ocean ridges, strain is accommodated by repeated episodes of faulting and magmatism. Discrete rifting episodes have been observed along two subaerial divergent plate boundaries, the Krafla segment of the Northern Volcanic Rift Zone in Iceland and the Manda-Hararo segment of the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03886

    authors: Passarelli L,Rivalta E,Shuler A

    更新日期:2014-01-28 00:00:00

  • Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2.

    abstract::Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptide designed to maximize antim...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17780-6

    authors: Mandell JB,Deslouches B,Montelaro RC,Shanks RMQ,Doi Y,Urish KL

    更新日期:2017-12-22 00:00:00

  • Bisacylphosphane oxides as photo-latent cytotoxic agents and potential photo-latent anticancer drugs.

    abstract::Bisacylphosphane oxides (BAPOs) are established as photoinitiators for industrial applications. Light irradiation leads to their photolysis, producing radicals. Radical species induce oxidative stress in cells and may cause cell death. Hence, BAPOs may be suitable as photolatent cytotoxic agents, but such applications...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42026-y

    authors: Beil A,Steudel FA,Bräuchle C,Grützmacher H,Möckl L

    更新日期:2019-04-12 00:00:00

  • Empirical Models of Shear-Wave Radiation Pattern Derived from Large Datasets of Ground-Shaking Observations.

    abstract::Shear-waves are the most energetic body-waves radiated from an earthquake, and are responsible for the destruction of engineered structures. In both short-term emergency response and long-term risk forecasting of disaster-resilient built environment, it is critical to predict spatially accurate distribution of shear-w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37524-4

    authors: Kotha SR,Cotton F,Bindi D

    更新日期:2019-01-30 00:00:00

  • PP2A(Cdc55)'s role in reductional chromosome segregation during achiasmate meiosis in budding yeast is independent of its FEAR function.

    abstract::PP2A(Cdc55) is a highly conserved serine-threonine protein phosphatase that is involved in diverse cellular processes. In budding yeast, meiotic cells lacking PP2A(Cdc55) activity undergo a premature exit from meiosis I which results in a failure to form bipolar spindles and divide nuclei. This defect is largely due t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30397

    authors: Kerr GW,Wong JH,Arumugam P

    更新日期:2016-07-26 00:00:00

  • Dendritic cells and macrophages neurally hard-wired in the lymph node.

    abstract::The neural hard-wired pathways in which the lymphoid organs are innervated by the nervous system is of special interest with respect to suggested afferent and sensory systems informing the central nervous system about the status of the immune system. Until today efferent also like afferent innervation seem to be unspe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16866

    authors: Wülfing C,Günther HS

    更新日期:2015-11-19 00:00:00

  • Phylogenomic analysis of MKKs and MAPKs from 16 legumes and detection of interacting pairs in chickpea divulge MAPK signalling modules.

    abstract::The mitogen-activated protein kinase (MAPK)-mediated phosphorylation cascade is a vital component of plant cellular signalling. Despite this, MAPK signalling cascade is less characterized in crop legumes. To fill this void, we present here a comprehensive phylogeny of MAPK kinases (MKKs) and MAPKs identified from 16 l...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04913-0

    authors: Purayannur S,Kumar K,Kaladhar VC,Verma PK

    更新日期:2017-07-10 00:00:00

  • Rottlerin-mediated inhibition of Toxoplasma gondii growth in BeWo trophoblast-like cells.

    abstract::Autophagy is a crucial and physiological process for cell survival from yeast to mammals, including protozoan parasites. Toxoplasma gondii, an intracellular parasite, typically exploits autophagic machinery of host cell; however host cell upregulates autophagy to combat the infection. Herein we tested the efficacy of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01525-6

    authors: Ietta F,Maioli E,Daveri E,Gonzaga Oliveira J,da Silva RJ,Romagnoli R,Cresti L,Maria Avanzati A,Paulesu L,Barbosa BF,Gomes AO,Roberto Mineo J,Ferro EAV

    更新日期:2017-04-28 00:00:00

  • A salivary metabolite signature that reflects gingival host-microbe interactions: instability predicts gingivitis susceptibility.

    abstract::Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59988-z

    authors: Fernandez-Gutierrez MM,Imangaliyev S,Prodan A,Loos BG,Keijser BJF,Kleerebezem M

    更新日期:2020-02-20 00:00:00

  • The effect of high glucose on lipid metabolism in the human placenta.

    abstract::Diabetes mellitus (DM) during pregnancy can result in fetal overgrowth, likely due to placental dysfunction, which has health consequences for the infant. Here we test our prediction from previous work using a placental cell line that high glucose concentrations affect placental lipid metabolism. Placentas from women ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50626-x

    authors: Hulme CH,Nicolaou A,Murphy SA,Heazell AEP,Myers JE,Westwood M

    更新日期:2019-10-01 00:00:00