Abstract:
:Cardiac contractility is enhanced by phosphorylation of myosin light chain 2 (MLC2) by cardiac-specific MLC kinase (cMLCK), located at the neck region of myosin heavy chain. In normal mouse and human hearts, the level of phosphorylation is maintained relatively constant, at around 30-40% of total MLC2, likely by well-balanced phosphorylation and phosphatase-dependent dephosphorylation. Overexpression of cMLCK promotes sarcomere organization, while the loss of cMLCK leads to cardiac atrophy in vitro and in vivo. In this study, we showed that cMLCK is predominantly expressed at the Z-disc with additional diffuse cytosolic expression in normal adult mouse and human hearts. cMLCK interacts with the Z-disc protein, α-actinin2, with a high-affinity kinetic value of 13.4 ± 0.1 nM through the N-terminus region of cMLCK unique to cardiac-isoform. cMLCK mutant deficient for interacting with α-actinin2 did not promote sarcomeric organization and reduced cardiomyocyte cell size. In contrast, a cMLCK kinase-deficient mutant showed effects similar to wild-type cMLCK on sarcomeric organization and cardiomyocyte cell size. Our results suggest that cMLCK plays a role in sarcomere organization, likely distinct from its role in phosphorylating MLC2, both of which will contribute to the enhancement of cardiac contractility.
journal_name
Sci Repjournal_title
Scientific reportsauthors
X Cai L,Tanada Y,D Bello G,C Fleming J,F Alkassis F,Ladd T,Golde T,Koh J,Chen S,Kasahara Hdoi
10.1038/s41598-019-48884-wsubject
Has Abstractpub_date
2019-08-29 00:00:00pages
12580issue
1issn
2045-2322pii
10.1038/s41598-019-48884-wjournal_volume
9pub_type
杂志文章abstract::Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07699
更新日期:2015-01-09 00:00:00
abstract::When a dot moves horizontally across a set of tilted lines of alternating orientations, the dot appears to be moving up and down along its trajectory. This perceptual phenomenon, known as the slalom illusion, reveals a mismatch between the veridical motion signals and the subjective percept of the motion trajectory, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64837-0
更新日期:2020-05-08 00:00:00
abstract::We present a highly ordered surface modification layer for polymers based on ambient solution-processed triptycene (Trip) derivatives for high-mobility organic thin-film transistors (OTFTs). The nested packing of Trip molecules results in the formation of 2D hexagonal arrays, which stack one-dimensionally on the surfa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45559-4
更新日期:2019-06-24 00:00:00
abstract::Human induced pluripotent stem cells (hiPSCs) are expected to be both a revolutionary cell source for regenerative medicine and a powerful tool to investigate the molecular mechanisms underlying human cell development in vitro. In the present study, we tried to elucidate the steroidogenic differentiation processes usi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15485-4
更新日期:2017-11-09 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-59766-x
更新日期:2020-02-11 00:00:00
abstract::Attention-deficit/hyperactivity disorder is frequently reported in individuals with Down syndrome, with considerable variation in the expression and severity of the symptoms. Despite growing evidence that gestational age predicts later symptoms of attention-deficit/hyperactivity disorder in the euploid population, thi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77392-5
更新日期:2020-11-23 00:00:00
abstract::Fatal Familial Insomnia (FFI) is a genetic prion disease caused by a point mutation in the prion protein gene (PRNP) characterized by prominent thalamic atrophy, diffuse astrogliosis and moderate deposition of PrPSc in the brain. Here, for the first time, we demonstrate that the olfactory mucosa (OM) of patients with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46269
更新日期:2017-04-07 00:00:00
abstract::Drug repositioning strategies have improved substantially in recent years. At present, two advances are poised to facilitate new strategies. First, the LINCS project can provide rich transcriptome data that reflect the responses of cells upon exposure to various drugs. Second, machine learning algorithms have been app...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07705-8
更新日期:2017-08-02 00:00:00
abstract::The coupling of the surface plasmon near-field into the sensing medium is key to the sensitivity of surface plasmon-based sensing devices. A low-index dielectric is necessary for the sensing medium to support a highly-penetrating surface plasmon evanescent field that extends well into the dielectric medium. The air-li...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38859-2
更新日期:2019-02-19 00:00:00
abstract::Over 80% of perinatal mortality in Japan is due to stillbirths after 22 weeks of gestation, with one in 300 families experiencing fetal loss every year. This study aimed to assess causes and risk factors for singleton stillbirth in Japan. A retrospective cross-sectional study was conducted using the Japan Society of O...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/s41598-018-22546-9
更新日期:2018-03-07 00:00:00
abstract::Phthalates are commonly included as ingredients in personal care products such as cosmetics, shampoos and perfumes. Diethyl phthalate (DEP) has been found to be anti-androgenic and linked with adverse reproductive effects on males, but effects on females are poorly understood. We designed an integrative and translatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63904-w
更新日期:2020-04-27 00:00:00
abstract::Visceral adiposity index (VAI) has been associated with various cardio-metabolic diseases; however, there is limited information about its association with cerebrovascular diseases. In this study, we evaluated the relationship between VAI and silent brain infarct (SBI). We evaluated a consecutive series of healthy vol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74454-6
更新日期:2020-10-14 00:00:00
abstract::Working memory (WM) impairments in ADHD have been consistently reported along with deficits in attentional control. Yet, it is not clear which specific WM processes are affected in this condition. A deficient coupling between attention and WM has been reported. Nevertheless, most studies focus on the capacity to retai...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64678-x
更新日期:2020-05-08 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG), a green tea-derived polyphenol, exhibits antitumor activities. An EGCG nanoemulsion (nano-EGCG) was prepared to improve the stability and reduce the side effects of EGCG for treatment of human lung cancer cells, and the antitumor effects were studied. The possible molecular mechanism...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62136-2
更新日期:2020-03-20 00:00:00
abstract::The bald eagle (Haliaeetus leucocephalus) once experienced near-extinction but has since rebounded. For decades, bald eagles near the Wisconsin River, USA, have experienced a lethal syndrome with characteristic clinical and pathological features but unknown etiology. Here, we describe a novel hepacivirus-like virus (F...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50580-8
更新日期:2019-10-18 00:00:00
abstract::Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted prot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45786-9
更新日期:2019-07-09 00:00:00
abstract::The emergence of multidrug-resistant Klebsiella pneumoniae is a worldwide problem. K. pneumoniae possesses numerous resistant genes in its genome. We isolated mutants resistant to various antimicrobials in vitro and investigated the importance of intrinsic genes in acquired resistance. The isolation frequency of the m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67820-x
更新日期:2020-07-02 00:00:00
abstract::Thaumatin is an intensely sweet-tasting protein that elicits sweet taste at a concentration of 50 nM, a value 100,000 times larger than that of sucrose on a molar basis. Here we attempted to produce a protein with enhanced sweetness by removing negative charges on the interacting side of thaumatin with the taste recep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20255
更新日期:2016-02-03 00:00:00
abstract::The microgravity environment during space flight imposes numerous adverse effects on animal and microbial physiology. It is unclear, however, how microgravity impacts those cellular interactions between mutualistic microbes and their hosts. Here, we used the symbiosis between the host squid Euprymna scolopes and its l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01340
更新日期:2013-01-01 00:00:00
abstract::Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these anim...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21143-0
更新日期:2018-02-12 00:00:00
abstract::While fluorescence microscopes and atomic force microscopes are widely used to visualize, track, and manipulate single biomolecules, the resolution of these methods is limited by sample drift. To minimize drift, active feedback methods have recently been used to stabilize single molecule microscopes on the sub-nanomet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32012-1
更新日期:2018-09-17 00:00:00
abstract::In higher plants, L-galactono-1,4-lactone dehydrogenase (GLDH) plays important roles in ascorbic acid (AsA) biosynthesis and assembly of respiration complex I. Here we report three homoeologous genes (TaGLDH-A1, -B1 and -D1) encoding common wheat GLDH isozymes and a unique allelic variant (TaGLDH-A1b) associated with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30177
更新日期:2016-07-22 00:00:00
abstract::PCTAIRE kinase 3 (PCTK3) is a member of the cyclin dependent kinase family, but its physiological function remains unknown. We previously reported that PCTK3-knockdown HEK293T cells showed actin accumulation at the leading edge, suggesting that PCTK3 is involved in the regulation of actin reorganization. In this study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45545
更新日期:2017-03-31 00:00:00
abstract::Carbon nanofibers were widely utilized to improve microwave absorption properties since they are a promising lightweight candidate. Adjustable conductive nanostructures of carbon nanofibers were synthesized by electrospinning technique. The conductive network is controlled by the polyvinyl pyrrolidone (PVP) content du...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38899-8
更新日期:2019-03-12 00:00:00
abstract::Autophagy is the major intracellular degradation system by which cytoplasmic materials are delivered to and degraded in the vacuole/lysosome in eukaryotic cells. MoAtg14 in M. oryzae, a hitherto uncharacterized protein, is the highly divergent homolog of the yeast Atg14 and the mammal BARKOR. The MoATG14 deletion muta...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40018
更新日期:2017-01-09 00:00:00
abstract::The C1q complement/TNF-related protein superfamily (CTRPs) displays differential effects on the regulation of metabolic homeostasis, governing cardiovascular function. However, whether and how they may serve as predictor/pro-diagnosis factors for assessing the risks of coronary artery disease (CAD) remains controversi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57877-z
更新日期:2020-01-21 00:00:00
abstract::Miscanthus is a promising lignocellulosic bioenergy crop for bioethanol production. To identify candidate genes and regulation networks involved in secondary cell wall (SCW) development in Miscanthus, we performed de novo transcriptome analysis of a developing internode. According to the histological and in-situ histo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08690-8
更新日期:2017-08-22 00:00:00
abstract::The Brazilian Urban Mobility Policy integrates urban transport with traffic planning, establishing appropriate public policies that indicate the need for a safe and accessible public transport system. The major challenge is the inclusion of the elderly and people with disabilities. In this paper, we quantify the impac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80906-w
更新日期:2021-01-12 00:00:00
abstract::We report the high susceptibility of several clinical isolates of Propionibacterium acnes from different sources (skin, bone, wound exudates, abscess or blood contamination) to the head-to-tail cyclized bacteriocin AS-48. This peptide is a feasible candidate for further pharmacological development against this bacteri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29580-7
更新日期:2018-08-06 00:00:00
abstract::The elemental two-dimensional (2D) materials such as graphene, silicene, germanene, and black phosphorus have attracted considerable attention due to their fascinating physical properties. Structurally they possess the honeycomb or distorted honeycomb lattices, which are composed of six-atom rings. Here we find a new ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25423
更新日期:2016-05-06 00:00:00