Method for high frequency tracking and sub-nm sample stabilization in single molecule fluorescence microscopy.

Abstract:

:While fluorescence microscopes and atomic force microscopes are widely used to visualize, track, and manipulate single biomolecules, the resolution of these methods is limited by sample drift. To minimize drift, active feedback methods have recently been used to stabilize single molecule microscopes on the sub-nanometer scale. However, these methods require high intensity lasers which limits their application in single molecule fluorescence measurements. Furthermore, these feedback methods do not track user-defined regions of the sample, but rather monitor the relative displacement of an unknown point on a fiducial marker, which limits their use in biological force measurements. To overcome these limitations, we have developed a novel method to image, track and stabilize a sample using low laser intensities. We demonstrate the capabilities of our approach by tracking a user-chosen point on a fiducial marker at 8.6 kHz and stabilizing it with sub-nanometer resolution. We further showcase the application of our method in single molecule fluorescence microscopy by imaging and stabilizing individual fluorescently-tagged streptavidin proteins under biologically relevant conditions. We anticipate that our method can be easily used to improve the resolution of a wide range of single molecule fluorescence microscopy and integrated force-fluorescence applications.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Schmidt PD,Reichert BH,Lajoie JG,Sivasankar S

doi

10.1038/s41598-018-32012-1

subject

Has Abstract

pub_date

2018-09-17 00:00:00

pages

13912

issue

1

issn

2045-2322

pii

10.1038/s41598-018-32012-1

journal_volume

8

pub_type

杂志文章
  • Subcellular localization of the sigma-1 receptor in retinal neurons - an electron microscopy study.

    abstract::The Sigma-1 receptor (S1R) is known to play a protective role in the central nervous system including the retina. A major barrier for understanding the underlying mechanism is an ambiguity of S1R subcellular localizations. We thus conducted the first electron microscopy (EM) study of S1R subcellular distribution in th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10689

    authors: Mavlyutov TA,Epstein M,Guo LW

    更新日期:2015-06-02 00:00:00

  • Differential photosynthetic and morphological adaptations to low light affect depth distribution of two submersed macrophytes in lakes.

    abstract::To evaluate the relative importance of photosynthetic versus morphological adaptations of submersed macrophytes to low light intensity in lakes, rapid light curves (RLCs), morphological parameters, relative growth rate (RGR), clonal reproduction and abundance of two submersed macrophytes (Potamogeton maackianus and Va...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34028

    authors: Chen J,Cao T,Zhang X,Xi Y,Ni L,Jeppesen E

    更新日期:2016-10-03 00:00:00

  • A Dual-reporter system for real-time monitoring and high-throughput CRISPR/Cas9 library screening of the hepatitis C virus.

    abstract::The hepatitis C virus (HCV) is one of the leading causes of chronic hepatitis, liver cirrhosis and hepatocellular carcinomas and infects approximately 170 million people worldwide. Although several reporter systems have been developed, many shortcomings limit their use in the assessment of HCV infections. Here, we rep...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08865

    authors: Ren Q,Li C,Yuan P,Cai C,Zhang L,Luo GG,Wei W

    更新日期:2015-03-09 00:00:00

  • Microcrack healing in non-ferrous metal tubes through eddy current pulse treatment.

    abstract::This study proposed a novel method to heal microcrack within Mg alloy tubes using high density eddy current pulse treatment (ECPT). Through electromagnetic induction inside a copper coil connected with a high density pulse power source supply, the high density (greater than 5 × 109 A/m2) and short duration eddy curren...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24354-7

    authors: Xu W,Yang C,Yu H,Jin X,Guo B,Shan D

    更新日期:2018-04-16 00:00:00

  • An activation force-based affinity measure for analyzing complex networks.

    abstract::Affinity measure is a key factor that determines the quality of the analysis of a complex network. Here, we introduce a type of statistics, activation forces, to weight the links of a complex network and thereby develop a desired affinity measure. We show that the approach is superior in facilitating the analysis thro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00113

    authors: Guo J,Guo H,Wang Z

    更新日期:2011-01-01 00:00:00

  • A tomato MADS-box protein, SlCMB1, regulates ethylene biosynthesis and carotenoid accumulation during fruit ripening.

    abstract::The MADS-box transcription factors play essential roles in many physiological and biochemical processes of plants, especially in fruit ripening. Here, a tomato MADS-box gene, SlCMB1, was isolated. SlCMB1 expression declined with the fruit ripening from immature green to B + 7 (7 days after Breaker) fruits in the wild ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21672-8

    authors: Zhang J,Hu Z,Yao Q,Guo X,Nguyen V,Li F,Chen G

    更新日期:2018-02-21 00:00:00

  • Experimental realization of controlled quantum teleportation of arbitrary qubit states via cluster states.

    abstract::Controlled quantum teleportation involves a third party as a controller for the teleportation of state. Here, we present the novel protocols for controlling teleportation of the arbitrary two-qubit and three-qubit states through five-qubit and seven-qubit cluster states respectively. In these schemes, Alice sends the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70446-8

    authors: Kumar A,Haddadi S,Pourkarimi MR,Behera BK,Panigrahi PK

    更新日期:2020-08-12 00:00:00

  • The number of key carcinogenic events can be predicted from cancer incidence.

    abstract::The widely accepted multiple-hit hypothesis of carcinogenesis states that cancers arise after several successive events. However, no consensus has been reached on the quantity and nature of these events, although "driver" mutations or epimutations are considered the most probable candidates. By using the largest publi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12448-7

    authors: Belikov AV

    更新日期:2017-09-22 00:00:00

  • Drivers of solar radiation variability in the McMurdo Dry Valleys, Antarctica.

    abstract::Annually averaged solar radiation in the McMurdo Dry Valleys, Antarctica has varied by over 20 W m-2 during the past three decades; however, the drivers of this variability are unknown. Because small differences in radiation are important to water availability and ecosystem functioning in polar deserts, determining th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23390-7

    authors: Obryk MK,Fountain AG,Doran PT,Lyons WB,Eastman R

    更新日期:2018-03-22 00:00:00

  • Publisher Correction: Antimicrobial spray nanocoating of supramolecular Fe(III)-tannic acid metal-organic coordination complex: applications to shoe insoles and fruits.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-37133-1

    authors: Park JH,Choi S,Moon HC,Seo H,Kim JY,Hong SP,Lee BS,Kang E,Lee J,Ryu DH,Choi IS

    更新日期:2018-12-14 00:00:00

  • Author Correction: Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-63750-w

    authors: Liu J,Huang W,Ren C,Wen Q,Liu W,Yang X,Wang L,Zhu B,Zeng L,Feng X,Zhang C,Chen H,Jia W,Zhang L,Xia X,Chen Y

    更新日期:2020-04-20 00:00:00

  • Comparative transcriptomics and host-specific parasite gene expression profiles inform on drivers of proliferative kidney disease.

    abstract::The myxozoan parasite, Tetracapsuloides bryosalmonae has a two-host life cycle alternating between freshwater bryozoans and salmonid fish. Infected fish can develop Proliferative Kidney Disease, characterised by a gross lymphoid-driven kidney pathology in wild and farmed salmonids. To facilitate an in-depth understand...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77881-7

    authors: Faber M,Shaw S,Yoon S,de Paiva Alves E,Wang B,Qi Z,Okamura B,Hartikainen H,Secombes CJ,Holland JW

    更新日期:2021-01-25 00:00:00

  • Brain volumetric alterations accompanied with loss of striatal medium-sized spiny neurons and cortical parvalbumin expressing interneurons in Brd1+/- mice.

    abstract::Schizophrenia is a common and severe mental disorder arising from complex gene-environment interactions affecting brain development and functioning. While a consensus on the neuroanatomical correlates of schizophrenia is emerging, much of its fundamental pathobiology remains unknown. In this study, we explore brain mo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-34729-5

    authors: Qvist P,Eskildsen SF,Hansen B,Baragji M,Ringgaard S,Roovers J,Paternoster V,Molgaard S,Corydon TJ,Stødkilde-Jørgensen H,Glerup S,Mors O,Wegener G,Nyengaard JR,Børglum AD,Christensen JH

    更新日期:2018-11-07 00:00:00

  • Localization-delocalization transition in spin-orbit-coupled Bose-Einstein condensate.

    abstract::We address the impact of the spin-orbit (SO) coupling on the localization-delocalization-transition (LDT) in a spin-orbit coupled Bose-Einstein condensate in a bichromatic potential. We find that SO coupling significantly alters the threshold depth of the one of sublattices above which the lowest eigenstates transform...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31700

    authors: Li C,Ye F,Kartashov YV,Konotop VV,Chen X

    更新日期:2016-08-17 00:00:00

  • A Clinically Relevant Functional Model of Type-2 Cardio-Renal Syndrome with Paraventricular Changes consequent to Chronic Ischaemic Heart Failure.

    abstract::Cardiorenal syndrome, de novo renal pathology arising secondary to cardiac insufficiency, is clinically recognised but poorly characterised. This study establishes and characterises a valid model representative of Type 2 cardiorenal syndrome. Extensive permanent left ventricular infarction, induced by ligation of the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58071-x

    authors: Harrison JC,Smart SDG,Besley EMH,Kelly JR,Read MI,Yao Y,Sammut IA

    更新日期:2020-01-27 00:00:00

  • Meta-analysis of soil mercury accumulation by vegetables.

    abstract::Mercury pollution in soil poses serious risks to human health through consumption of contaminated vegetables. We used a meta-analysis to examine the mercury enrichment ability of different vegetables and the main factors affecting mercury uptake. We drew the following conclusions. (1) Plants with a lower bioconcentrat...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-018-19519-3

    authors: Yu H,Li J,Luan Y

    更新日期:2018-01-19 00:00:00

  • Male mice with large inversions or deletions of X-chromosome palindrome arms are fertile and express their associated genes during post-meiosis.

    abstract::Large (>10 kb) palindromic sequences are enriched on mammalian sex chromosomes. In mice, these palindromes harbor gene families (≥2 gene copies) expressed exclusively in post-meiotic testicular germ cells, a time when most single-copy sex-linked genes are transcriptionally repressed. This observation led to the hypoth...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27360-x

    authors: Kruger AN,Ellison Q,Brogley MA,Gerlinger ER,Mueller JL

    更新日期:2018-06-12 00:00:00

  • Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9.

    abstract::Differential vulnerability among motor neuron (MN) subtypes is a fundamental feature of amyotrophic lateral sclerosis (ALS): fast-fatigable (FF) MNs are more vulnerable than fast fatigue-resistant (FR) or slow (S) MNs. The reason for this selective vulnerability remains enigmatic. We report here that the extracellular...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27354

    authors: Morisaki Y,Niikura M,Watanabe M,Onishi K,Tanabe S,Moriwaki Y,Okuda T,Ohara S,Murayama S,Takao M,Uchida S,Yamanaka K,Misawa H

    更新日期:2016-06-06 00:00:00

  • Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures.

    abstract::Plant cell cultures are an attractive platform for the production of recombinant proteins. A major drawback, hindering the establishment of plant cell suspensions as an industrial platform, is the low product yield obtained thus far. Histone acetylation is associated with increased transcription levels, therefore it i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17006-9

    authors: Santos RB,Pires AS,Abranches R

    更新日期:2017-12-01 00:00:00

  • Interplay between TETs and microRNAs in the adult brain for memory formation.

    abstract::5-hydroxymethylation (5-hmC) is an epigenetic modification on DNA that results from the conversion of 5-methylcytosine by Ten-Eleven Translocation (TET) proteins. 5-hmC is widely present in the brain and is subjected to dynamic regulation during development and upon neuronal activity. It was recently shown to be invol...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19806-z

    authors: Kremer EA,Gaur N,Lee MA,Engmann O,Bohacek J,Mansuy IM

    更新日期:2018-01-26 00:00:00

  • Reproductive performance of gilthead seabream (Sparus aurata) broodstock showing different expression of fatty acyl desaturase 2 and fed two dietary fatty acid profiles.

    abstract::Previous studies have shown that it is possible to nutritionally program gilthead seabream offspring through fish oil (FO) replacement by vegetable oils (VO) in the broodstock diet, to improve their ability to grow fast when fed low fish meal (FM) and FO diets during grow-out phase. However, in those studies broodstoc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72166-5

    authors: Ferosekhan S,Xu H,Turkmen S,Gómez A,Afonso JM,Fontanillas R,Rosenlund G,Kaushik S,Izquierdo M

    更新日期:2020-09-23 00:00:00

  • Highly Interactive Brain-Computer Interface Based on Flicker-Free Steady-State Motion Visual Evoked Potential.

    abstract::Visual evoked potential-based brain-computer interfaces (BCIs) have been widely investigated because of their easy system configuration and high information transfer rate (ITR). However, the uncomfortable flicker or brightness modulation of existing methods restricts the practical interactivity of BCI applications. In...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24008-8

    authors: Han C,Xu G,Xie J,Chen C,Zhang S

    更新日期:2018-04-11 00:00:00

  • Scanning tunneling microscopy on cleaved Mn3Sn(0001) surface.

    abstract::We have studied in-situ cleaved (0001) surfaces of the magnetic Weyl semimetal Mn3Sn by low-temperature scanning tunneling microscopy and spectroscopy (STM/S). It was found that freshly cleaved Mn3Sn surfaces are covered with unknown clusters, and the application of voltage pulses in the tunneling condition was needed...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45958-7

    authors: Yang HH,Lee CC,Yoshida Y,Ikhlas M,Tomita T,Nugroho A,Ozaki T,Nakatsuji S,Hasegawa Y

    更新日期:2019-07-04 00:00:00

  • Antifibrotic effect of novel neutrophil gelatinase-associated lipocalin inhibitors in cardiac and renal disease models.

    abstract::Neutrophil gelatinase-associated lipocalin (NGAL) is involved in cardiovascular and renal diseases. Gene inactivation of NGAL blunts the pathophysiological consequences of cardiovascular and renal damage. We aimed to design chemical NGAL inhibitors and investigate its effects in experimental models of myocardial infar...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-82279-0

    authors: Bonnard B,Martínez-Martínez E,Fernández-Celis A,Pieronne-Deperrois M,Do QT,Ramos I,Rossignol P,Zannad F,Mulder P,Ouvrard-Pascaud A,López-Andrés N,Jaisser F

    更新日期:2021-01-28 00:00:00

  • Demonstration of surface transport in a hybrid Bi2Se3/Bi2Te3 heterostructure.

    abstract::In spite of much work on topological insulators (TIs), systematic experiments for TI/TI heterostructures remain absent. We grow a high quality heterostructure containing single quintuple layer (QL) of Bi2Se3 on 19 QLs of Bi2Te3 and compare its transport properties with 20 QLs Bi2Se3 and 20 QLs Bi2Te3. All three films ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03060

    authors: Zhao Y,Chang CZ,Jiang Y,DaSilva A,Sun Y,Wang H,Xing Y,Wang Y,He K,Ma X,Xue QK,Wang J

    更新日期:2013-10-28 00:00:00

  • All semiconductor enhanced high-harmonic generation from a single nanostructured cone.

    abstract::The enhancement and control of non-linear phenomena at a nanometer scale has a wide range of applications in science and in industry. Among these phenomena, high-harmonic generation in solids is a recent focus of research to realize next generation petahertz optoelectronic devices or compact all solid state EUV source...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41642-y

    authors: Franz D,Kaassamani S,Gauthier D,Nicolas R,Kholodtsova M,Douillard L,Gomes JT,Lavoute L,Gaponov D,Ducros N,Février S,Biegert J,Shi L,Kovacev M,Boutu W,Merdji H

    更新日期:2019-04-05 00:00:00

  • Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe.

    abstract::In this study we describe a new methodology to physically probe individual complexes formed between proteins and DNA. By combining nanoscale, high speed physical force measurement with sensitive fluorescence imaging we investigate the complex formed between the prokaryotic DNA repair protein UvrA2 and DNA. This approa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep18486

    authors: Simons M,Pollard MR,Hughes CD,Ward AD,Van Houten B,Towrie M,Botchway SW,Parker AW,Kad NM

    更新日期:2015-12-22 00:00:00

  • Characterization of a novel reassortant influenza A virus (H2N2) from a domestic duck in Eastern China.

    abstract::While H2N2 viruses have been sporadically isolated from wild and domestic birds, H2N2 viruses have not been detected among human populations since 1968. Should H2N2 viruses adapt to domestic poultry they may pose a risk of infection to people, as most anyone born after 1968 would likely be susceptible to their infecti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07588

    authors: Ma MJ,Yang XX,Qian YH,Zhao SY,Hua S,Wang TC,Chen SH,Ma GY,Sang XY,Liu LN,Wu AP,Jiang TJ,Gao YW,Gray GC,Zhao T,Ling X,Wang JL,Lu B,Qian J,Cao WC

    更新日期:2014-12-23 00:00:00

  • Synergistic effects of tung oil and heat treatment on physicochemical properties of bamboo materials.

    abstract::The search for green and sustainable modification method to produce durable bamboo materials remains a challenge in industry. Here, heat treatment in tung oil at 100-200 °C was employed to modify bamboo materials. Oil permeation and distribution in the structure of bamboo samples during heat treatment were explored. T...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49240-8

    authors: Tang T,Zhang B,Liu X,Wang W,Chen X,Fei B

    更新日期:2019-09-06 00:00:00

  • Fragment-based screening identifies inhibitors of ATPase activity and of hexamer formation of Cagα from the Helicobacter pylori type IV secretion system.

    abstract::Type IV secretion systems are multiprotein complexes that mediate the translocation of macromolecules across the bacterial cell envelope. In Helicobacter pylori a type IV secretion system encoded by the cag pathogenicity island encodes 27 proteins and most are essential for virulence. We here present the identificatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42876-6

    authors: Arya T,Oudouhou F,Casu B,Bessette B,Sygusch J,Baron C

    更新日期:2019-04-24 00:00:00