Abstract:
:The emergence of multidrug-resistant Klebsiella pneumoniae is a worldwide problem. K. pneumoniae possesses numerous resistant genes in its genome. We isolated mutants resistant to various antimicrobials in vitro and investigated the importance of intrinsic genes in acquired resistance. The isolation frequency of the mutants was 10-7-10-9. Of the multidrug-resistant mutants, hyper-multidrug-resistant mutants (EB256-1, EB256-2, Nov1-8, Nov2-2, and OX128) were identified, and accelerated efflux activity of ethidium from the inside to the outside of the cells was observed in these mutants. Therefore, we hypothesized that the multidrug efflux pump, especially RND-type efflux pump, would be related to changes of the phenotype. We cloned all RND-type multidrug efflux pumps from the K. pneumoniae genome and characterized them. KexEF and KexC were powerful multidrug efflux pumps, in addition to AcrAB, KexD, OqxAB, and EefABC, which were reported previously. It was revealed that the expression of eefA was increased in EB256-1 and EB256-2: the expression of oqxA was increased in OX128; the expression of kexF was increased in Nov2-2. It was found that a region of 1,485 bp upstream of kexF, was deleted in the genome of Nov2-2. K. pneumoniae possesses more potent RND-multidrug efflux systems than E. coli. However, we revealed that most of them did not contribute to the drug resistance of our strain at basic levels of expression. On the other hand, it was also noted that the overexpression of these pumps could lead to multidrug resistance based on exposure to antimicrobial chemicals. We conclude that these pumps may have a role to maintain the intrinsic resistance of K. pneumoniae when they are overexpressed. The antimicrobial chemicals selected many resistant mutants at the same minimum inhibitory concentration (MIC) or a concentration slightly higher than the MIC. These results support the importance of using antibiotics at appropriate concentrations at clinical sites.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ni RT,Onishi M,Mizusawa M,Kitagawa R,Kishino T,Matsubara F,Tsuchiya T,Kuroda T,Ogawa Wdoi
10.1038/s41598-020-67820-xsubject
Has Abstractpub_date
2020-07-02 00:00:00pages
10876issue
1issn
2045-2322pii
10.1038/s41598-020-67820-xjournal_volume
10pub_type
杂志文章abstract::A photovoltaic reactor was designed for artificial photosynthesis, based on the reactions involved in high energy hydrogen atoms, which were produced from water electrolysis. Water and CO2, under the conditions studied, were converted to oxalate (H2C2O4) and a polymer. This was the first time that the oxalates and oxa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03572
更新日期:2014-01-06 00:00:00
abstract::Crops expressing Bacillus thuringiensis (Bt)-derived insecticidal protein genes have been commercially available for over 15 years and are providing significant value to growers. However, there remains the need for alternative insecticidal actives due to emerging insect resistance to certain Bt proteins. A screen of b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03544-9
更新日期:2017-06-08 00:00:00
abstract::The application of a lattice structure in the lightweight design of compressor impellers can reduce their mass and moment of inertia, hence improving the effective carrying of aircraft and reducing the start and braking moments of the impeller. The feasibility of a processing-lattice compressor impeller is the prerequ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57131-1
更新日期:2020-01-17 00:00:00
abstract::Green microalgae contain many active pigments such as carotenoids having antioxidant and protective activity on human cells. Here we investigate the biological activity of an ethanol/water extract of the marine green microalga Tetraselmis suecica containing high levels of carotenoids such as the xanthophylls lutein, v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41215
更新日期:2017-01-24 00:00:00
abstract::Expression of the mismatch repair gene MutL homolog 1 (MLH1) is silenced in a clinically important subgroup of sporadic colorectal cancers. These cancers exhibit hypermutability with microsatellite instability (MSI) and differ from microsatellite-stable (MSS) colorectal cancers in both prognosis and response to therap...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-019-49952-x
更新日期:2019-09-17 00:00:00
abstract::Chondrichthyes - sharks, rays, skates, and chimeras, are among the most threatened and data deficient vertebrate species. Global demand for shark and ray derived products, drives unregulated and exploitative fishing practices, which are in turn facilitated by the lack of ecological data required for effective conserva...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40940-9
更新日期:2019-03-14 00:00:00
abstract::Decarboxylation of fatty acids over non-noble metal catalysts without added hydrogen was studied. Ni/C catalysts were prepared and exhibited excellent activity and maintenance for decarboxylation. Thereafter, the effects of nickel loading, catalyst loading, temperature, and carbon number on the decarboxylation of fatt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27820
更新日期:2016-06-13 00:00:00
abstract::For many malignancies, inflammation-based scores correlate with survival. The neutrophil-to-lymphocyte ratio (NLR) and prognostic nutritional index (PNI) are immunonutritional indices associated with postoperative outcomes in patients with hepatocellular carcinoma (HCC). We evaluated whether a combined preoperative NL...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13239-w
更新日期:2017-10-24 00:00:00
abstract::Arctic amplification of global warming has led to increased summer sea ice retreat, which influences gas exchange between the Arctic Ocean and the atmosphere where sea ice previously acted as a physical barrier. Indeed, recently observed enhanced atmospheric methane concentrations in Arctic regions with fractional sea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16179
更新日期:2015-11-10 00:00:00
abstract::Drug addiction remains a prevalent and fatal disease worldwide that carries significant social and economic impacts. Recent reports suggest illicit pregabalin (Lyrica) use may be increasing among youth, however the addictive potential of pregabalin has not been well established. Drug seeking behavior and chronic drug ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51556-4
更新日期:2019-10-22 00:00:00
abstract::We report the results of an experiment on radio-tracking of individual grey field slugs in an arable field and associated data modelling designed to investigate the effect of slug population density in their movement. Slugs were collected in a commercial winter wheat field in which a 5x6 trapping grid had been establi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74643-3
更新日期:2020-10-21 00:00:00
abstract::Adaptive evolution by serial subcultivation of co-cultured Bacillus thuringiensis and Ketogulonicigenium vulgare significantly enhanced the productivity of 2-keto-L-gulonic acid in two-step vitamin C production. The adaptation mechanism in K. vulgare-B. thuringiensis consortium was investigated in this study based on ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46759
更新日期:2017-04-25 00:00:00
abstract::This study aimed to identify single nucleotide polymorphisms (SNPs) associated with milk cholesterol (CHL) content via a genome wide association study (GWAS). Milk CHL content was determined by gas chromatography and expressed as mg of CHL in 100 g of fat (CHL_fat) or in 100 mg of milk (CHL_milk). GWAS was performed w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31427-0
更新日期:2018-09-05 00:00:00
abstract::A novel label-free electrochemiluminescence (ECL) immunosensor based on luminol functional-Au NPs@polypyrrole has been developed for the detection of carcinoembryonic antigen (CEA). In this work, polypyrrole prepared by chemical polymerization provided a large surface area to load amounts of gold nanoparticles (Au NPs...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24599
更新日期:2016-04-19 00:00:00
abstract::Protein aggregation is a common biological phenomenon, observed in different physiological and pathological conditions. Decreased protein solubility and a tendency to aggregate is also observed during physiological aging but the causes are currently unknown. Herein we performed a biophysical separation of aging-relate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19311
更新日期:2016-01-18 00:00:00
abstract::Pertuzumab is an antihuman HER2 antibody developed for HER2 positive breast cancer. Glycosylation profiles are always the important issue for antibody based therapy. Previous findings have suggested the impact of glycosylation profiles on the function of antibodies, like pharmacodynamics, antibody-dependent cellular c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46347
更新日期:2017-04-11 00:00:00
abstract::The estrogenicity of parabens at human exposure levels has become a focus of concern due to the debate over whether the estrogenicity of parabens is strong enough to play a role in the increased incidence of breast cancer. In this study, the uterotrophic activities of methylparaben (MP) and ethylparaben (EP) at doses ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25173
更新日期:2016-04-28 00:00:00
abstract::Charge density wave (CDW), the periodic modulation of the electronic charge density, will open a gap on the Fermi surface that commonly leads to decreased or vanishing conductivity. On the other hand superconductivity, a commonly believed competing order, features a Fermi surface gap that results in infinite conductiv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26974
更新日期:2016-06-02 00:00:00
abstract::Podocyte injury has been suggested to induce phenotypic alteration of glomerular podocytes and accelerate the detachment of podocytes from the glomeruli resulting in podocyturia. However, it is not clear whether podocyte phenotypic alteration occurs in the urine of women with preeclampsia (PE). Seventy-seven and 116 p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24258
更新日期:2016-04-07 00:00:00
abstract::The relative and combined effects of sleep apnea with diabetes mellitus (DM) on cardiovascular outcomes in patients undergoing coronary artery bypass grafting (CABG) remain unknown. In this secondary analysis of data from the SABOT study, 1007 patients were reclassified into four groups based on their sleep apnea and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78700-9
更新日期:2020-12-10 00:00:00
abstract::Amphibian oocytes have been extensively used for heterologous expression of membrane proteins for studying their biochemical and biophysical properties. So far, Xenopus laevis is the main amphibian used as oocytes source to express aquaglyceroporins in order to assess water and solutes permeability. However, this well...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37069-6
更新日期:2019-01-10 00:00:00
abstract::Drug discovery efforts against the pathogen Mycobacterium tuberculosis (Mtb) have been advanced through phenotypic screens of extensive compound libraries. Such a screen revealed sulfolane 1 and indoline-5-sulfonamides 2 and 3 as potent inhibitors of mycobacterial growth. Optimization in the sulfolane series led to co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09642-y
更新日期:2017-08-25 00:00:00
abstract::Aiming to promote low-cost production of protein-rich forage rice and resource recycling from wastewater treatment plants, a pot experiment was conducted to assess the possibility to substitute mineral fertilizers with composted sewage sludge (CSS) with/without top-dressing with treated municipal wastewater (TWW). Res...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67233-w
更新日期:2020-06-23 00:00:00
abstract::A considerable body of evidence links together mitochondrial dysfunctions, toxic action of metalloid oxyanions, and system and neurodegenerative disorders. In this study we have used the model yeast Saccharomyces cerevisiae to investigate the genetic determinants associated with tellurite resistance/sensitivity. Nitro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30479-6
更新日期:2018-08-13 00:00:00
abstract::Human protein tyrosine phosphatase non-receptor type 4 (PTPN4) has been shown to prevent cell death. The active form of human PTPN4 consists of two globular domains, a PDZ (PSD-95/Dlg/ZO-1) domain and a phosphatase domain, tethered by a flexible linker. Targeting its PDZ domain abrogates this protection and triggers a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08193-6
更新日期:2017-08-11 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-56284-3
更新日期:2019-12-16 00:00:00
abstract::The small intestine plays an essential role in the health and well-being of animals. Previous studies have shown that Lactobacillus has a protective effect on intestinal morphology, intestinal epithelium integrity and appropriate maturation of gut-associated tissues. Here, gene expression in jejunum tissue of weaned p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16158-y
更新日期:2017-11-17 00:00:00
abstract::Non-availability of an ideal alternative for autografts in treating critical-size bone defects is a major challenge in orthopedics. Phytocompounds have been proven to enhance osteogenesis via various osteogenic signaling pathways, but its decreased bioavailability and increased renal clearance limit its application. I...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48429-1
更新日期:2019-08-15 00:00:00
abstract::Both CC and CXC-class chemokines drive inflammatory disease. Tick salivary chemokine-binding proteins (CKBPs), or evasins, specifically bind subsets of CC- or CXC-chemokines, and could precisely target disease-relevant chemokines. Here we have used yeast surface display to identify two tick evasins: a CC-CKBP, P1243 f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24568-9
更新日期:2018-04-20 00:00:00
abstract::The d-band center model of Hammer and Nørskov is widely used in understanding and predicting catalytic activity on transition metal (TM) surfaces. Here, we demonstrate that this model is inadequate for capturing the complete catalytic activity of the magnetically polarized TM surfaces and propose its generalization. W...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35916
更新日期:2016-11-03 00:00:00