Abstract：
:Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted protein in Phaeodactylum tricornutum, designated 'highly abundant secreted protein 1' (HASP1), and characterised the activities of its promoter and signal peptide using green fluorescent protein (GFP) as a reporter. The HASP1 promoter strongly drove GFP expression during all growth phases of P. tricornutum in culture, in contrast to the commonly used fcpA promoter, which is less active during the stationary phase. The HASP1 signal peptide was also sufficient for facilitating efficient secretion of GFP by P. tricornutum. Our findings suggest that both the promoter and the signal peptide of HASP1 can be utilized as novel tools for the overexpression and secretion of recombinant proteins in P. tricornutum.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Erdene-Ochir E,Shin BK,Kwon B,Jung C,Pan CHdoi
10.1038/s41598-019-45786-9subject
Has Abstractpub_date
2019-07-09 00:00:00pages
9941issue
1issn
2045-2322pii
10.1038/s41598-019-45786-9journal_volume
9pub_type
杂志文章abstract：:Parkinson's Disease can be understood as a disorder of motor habits. A prediction of this theory is that early stage Parkinson's patients will display fewer errors caused by interference from previously over-learned behaviours. We test this prediction in the domain of skilled typing, where actions are easy to record a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39294-z
更新日期：2019-03-04 00:00:00
abstract：:The objective of this meta-analysis is to compare hybrid surgery (HS) and cervical discectomy and fusion (ACDF) for multilevel cervical degenerative disc diseases (DDD). Systematic searches of all published studies through March 2015 were identified from Cochrane Library, Medline, PubMed, Embase, ScienceDirect, CNKI, ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep13454
更新日期：2015-08-26 00:00:00
abstract：:In this paper, a novel explanation on high directive emission of Fabry-Perot resonator antenna with subwavelength metasurface is proposed. Based on image theory and effective constitutive parameter retrieval, the whole Fabry-Perot resonant cavity structure composed of a single-layer metasurface with square ring elemen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11141-z
更新日期：2017-09-13 00:00:00
abstract：:Oligomannose-type glycans on HIV-1 gp120 form a patch that is targeted by several broadly neutralizing antibodies (bnAbs) and that therefore is of interest to vaccine design. However, attempts to elicit similar oligomannose-specific bnAbs by immunizing with oligomannosidic glycoconjugates have only been modestly succe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64500-8
更新日期：2020-05-05 00:00:00
abstract：:Calibration of quantum computers is essential to the effective utilisation of their quantum resources. Specifically, the performance of quantum annealers is likely to be significantly impaired by noise in their programmable parameters, effectively misspecification of the computational problem to be solved, often resul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18628
更新日期：2016-01-19 00:00:00
abstract：:Colorectal cancer (CRC) is a common complex disease caused by the combination of genetic variants and environmental factors. Genome-wide association studies (GWAS) have been performed and reported some novel CRC susceptibility variants. However, the potential genetic mechanisms for newly identified CRC susceptibility ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25347
更新日期：2016-05-05 00:00:00
abstract：:The combustion of candles exhibits a variety of dynamical behaviors. Binding several candles together will result in flickering of candle flames, which is generally described as a nonlinear oscillator. The impact on the frequency of the flame by several factors, such as the arrangement, the number and the asymmetry of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36754-w
更新日期：2019-01-23 00:00:00
abstract：:Synthesis of homogenous glycans in quantitative yields represents a major bottleneck to the production of molecular tools for glycoscience, such as glycan microarrays, affinity resins, and reference standards. Here, we describe a combined biological/enzymatic synthesis that is capable of efficiently converting microbi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15891-8
更新日期：2017-11-21 00:00:00
abstract：:Various important topological properties of healthy brain connectome have recently been identified. However, the manner in which brain lesion changes the functional network topology is unknown. We examined how critical specific brain areas are in the maintenance of network topology using multivariate support vector re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17886-x
更新日期：2017-12-20 00:00:00
abstract：:Serratia marcescens is one of the important nosocomial pathogens which rely on quorum sensing (QS) to regulate the production of biofilm and several virulence factors. Hence, blocking of QS has become a promising approach to quench the virulence of S. marcescens. For the first time, QS inhibitory (QSI) and antibiofilm...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16507-x
更新日期：2017-11-27 00:00:00
abstract：:Association of a high-serum ferritin with poor outcome showed that iron might play a detrimental role in the brain after intracerebral hemorrhage (ICH). Here, we investigated changes in serum iron, ferritin, transferrin (Tf) and ceruloplasmin (CP) in patients with ICH (n = 100) at day 1 (admission), 3, 7, 14 and 21 an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21970
更新日期：2016-02-22 00:00:00
abstract：:Calcium plays a role of universal cellular regulator in the living cell and one of the crucial regulators of proper fetal development during gestation. Mitochondria are important for intracellular calcium handling and signaling. Mitochondrial calcium uniporter (mtCU) is a multiprotein complex of the mitochondrial inne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41996-3
更新日期：2019-04-02 00:00:00
abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in thousands of deaths in the world. Information about prediction model of prognosis of SARS-CoV-2 infection is scarce. We used machine learning for processing laboratory findings of 110 patients with SARS-CoV-2 pneumonia (including 51 non-s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71114-7
更新日期：2020-08-20 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is an important risk factor for the development of type 2 diabetes mellitus. Interferon gamma-induced protein 10 (IP-10), a proinflammatory chemokine, plays a crucial role in inflammatory diseases. This cross-sectional pilot study investigated whether circulating IP-10 is assoc...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/srep10096
更新日期：2015-05-11 00:00:00
abstract：:A high-performance and novel photocatalyst of BiVO4-reduced Graphene Oxide (BiVO4-rGO) nanocomposite was prepared by a facile hydrothermal method. The photocatalyst was characterized by X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electronic microscopy, UV-Vis diffusi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07342-1
更新日期：2017-08-11 00:00:00
abstract：:Urbanization is associated with dramatic increases in noise and light pollution, which affect animal behaviour, physiology and fitness. However, few studies have examined these stressors simultaneously. Moreover, effects of urbanization during early-life may be detrimental but are largely unknown. In developing great ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02940-5
更新日期：2017-06-05 00:00:00
abstract：:Fluid overload (FO) has been shown to adversely affect multiple organs and survival in critically ill patients. Liver transplantation (LT) carries the risk of massive transfusion, which frequently results in FO. We investigated the association of postoperative weight gain with graft failure, early allograft dysfunctio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56543-3
更新日期：2019-12-27 00:00:00
abstract：:An increase of nucleolar number and size has made nucleoli essential markers for cytology and tumour development. However, the underlying basis for their structural integrity and abundance remains unclear. Protein phosphatase PPM1D was found to be up-regulated in different carcinomas including breast cancers. Here, we...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33272
更新日期：2016-09-13 00:00:00
abstract：:SARS-CoV-2 can transmit efficiently in humans, but it is less clear which other mammals are at risk of being infected. SARS-CoV-2 encodes a Spike (S) protein that binds to human ACE2 receptor to mediate cell entry. A species with a human-like ACE2 receptor could therefore be at risk of being infected by SARS-CoV-2. We...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80573-x
更新日期：2021-01-18 00:00:00
abstract：:With the reduction of oil and gas reserves and the increase of mining difficulty in Northern China, the carbonate rocks in Southern North China Basin are becoming a significant exploration target for carbonate reservoirs. However, the development characteristics, formation stages, formation environments and mechanisms...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80878-x
更新日期：2021-01-12 00:00:00
abstract：:Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13991
更新日期：2015-09-21 00:00:00
abstract：:Physical activity (PA) is known to influence cognitive function. However, the impact of PA on patients with cerebrovascular disease (CVD) has not yet been elucidated. PA and cognitive function have not been measured simultaneously over time, which makes it difficult to evaluate their relationship. The purpose of this ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40460-6
更新日期：2019-03-07 00:00:00
abstract：:The presence of a disease continuum in inflammatory arthritis (IA) is a recognised concept, with distinct stages from at-risk stage (presence of anti citrullinated-peptide autoantibody) to diagnosis of rheumatoid arthritis (RA), including therapy-induced remission. Despite T-cell dysregulation being a key feature of R...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/s41598-020-60314-w
更新日期：2020-02-28 00:00:00
abstract：:By using highly aligned carbon nanotube (CNT) sheets of excellent optical transmittance and mechanical stretchability as both the current collector and active electrode, high-performance transparent and stretchable all-solid supercapacitors with a good stability were developed. A transmittance up to 75% at the wavelen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03612
更新日期：2014-01-09 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-67129-9
更新日期：2020-06-17 00:00:00
abstract：:The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: di...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42121
更新日期：2017-02-07 00:00:00
abstract：:Noise-induced hearing loss (NIHL) is a common health concern with significant social, psychological, and cognitive implications. Moderate levels of acoustic overstimulation associated with tinnitus and impaired speech perception cause cochlear synaptopathy, characterized physiologically by reduction in wave I of the s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75058-w
更新日期：2020-10-22 00:00:00
abstract：:The cuticle of the beetle Chrysina chrysargyrea reflects left-handed polarized light in the broad spectral range from 340 to 1000 nm. Interference oscillations in the experimental Mueller-matrix spectroscopic ellipsometry data reveal that transparent materials comprise the cuticle. A spectral analysis of the interfere...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24761-w
更新日期：2018-04-24 00:00:00
abstract：:In the central nervous system of vertebrates, cell bodies of neurons are often assembled as nuclei or cellular layers that play specific roles as functional units. The purpose of this work was to selectively highlight such cell assemblies by magnetic resonance imaging using signals from water protons that are associat...
journal_title：Scientific reports
pub_type： 杂志文章,评审
doi：10.1038/s41598-019-41587-2
更新日期：2019-03-25 00:00:00
abstract：:The biological function of multiple repetitions of single amino acids, or homo-repeats, is largely unknown, but their occurrence in proteins has been associated with more than 20 hereditary diseases. Analysing 122 bacterial and eukaryotic genomes, we observed that the number of proteins containing homo-repeats is sign...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26941
更新日期：2016-06-03 00:00:00
