Abstract：
:Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca(2+) and cAMP levels of live embryos expressing dual FRET sensors can be monitored simultaneously at microscopic resolution. The method is compatible with a broad range of FRET sensors currently available for probing various cellular biochemical functions. It opens the door to imaging complex cellular circuitries in whole live organisms.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhao M,Wan X,Li Y,Zhou W,Peng Ldoi
10.1038/srep13991subject
Has Abstractpub_date
2015-09-21 00:00:00pages
13991issn
2045-2322pii
srep13991journal_volume
5pub_type
杂志文章abstract：:Enterovirus A71 (EV-A71), which belongs to the family Picornaviridae, can invade the central nervous system (CNS) and cause severe CNS complications or death. The EV-A71 antigen has been detected in the neurons in the brains of humans who died from EV-A71 infection. However, the effect of EV-A71 infection on human neu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71970-3
更新日期：2020-09-17 00:00:00
abstract：:Systemic lupus erythematosus (SLE) patients are vulnerable to infections. We aim to explore the approach to differentiate active infection from disease activity in pediatric SLE patients. Fifty pediatric SLE patients presenting with 185 clinical visits were collected. The associations between both clinical and laborat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76789-6
更新日期：2020-11-16 00:00:00
abstract：:We developed a new concept of X- and γ-ray radiation semiconductor detectors based on a large area graphene/semi-insulating single crystal CdTe Schottky-type heterojunction. These two terminal electronic devices can be easily fabricated by forming a Van der Waals contact between large area chemical vapor deposited gra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37637-w
更新日期：2019-01-31 00:00:00
abstract：:The removal of macroalgal biomass is critical to the health of coral reef ecosystems. Previous studies on relatively intact reefs with diverse and abundant fish communities have quantified rapid removal of macroalgae by herbivorous fishes, yet how these findings relate to degraded reef systems where fish diversity and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08873-3
更新日期：2017-08-21 00:00:00
abstract：:The focus of this study is to evaluate the efficacy of Machine Learning (ML) algorithms in the long-lead prediction of El Niño (La Niña) Modoki (ENSO Modoki) index (EMI). We evaluated two widely used non-linear ML algorithms namely Support Vector Regression (SVR) and Random Forest (RF) to forecast the EMI at various l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57183-3
更新日期：2020-01-15 00:00:00
abstract：:We proposed a simple model for generation of controllable ultraslow optical solitons of a weak probe laser light in a degenerated two-level atomic medium under electromagnetically induced transparency assisted by a magnetic field. It is shown that bright and dark optical solitons can be formed from a probe light with ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72256-4
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abstract：:Due to the high CO2-footprint of ordinary Portland cement (OPC), the search for alternative binders is now in a full swing. Rankinite-which is a hydraulically inactive material and low in calcium, is a real alternative to OPC, as it absorbs the harmful greenhouse gas from the air through carbonation hardening. Neverth...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-09-02 00:00:00
abstract：:Enhancement of endogenous neurogenesis after ischemic stroke may improve functional recovery. We previously demonstrated that medium B, which is a combination with epidermal growth factor (EGF) and fibronectin, can promote neural stem/progenitor cell (NSPC) proliferation and migration. Here, we showed that medium B pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63598-0
更新日期：2020-04-20 00:00:00
abstract：:The effect of hypoglycemia on the progression of atherosclerosis in patients with type 2 diabetes mellitus (T2DM) remains largely unknown. This is a post hoc analysis of a randomized trial to investigate the relationship between hypoglycemic episodes and changes in carotid intima-media thickness (IMT). Among 274 study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39965
更新日期：2017-01-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00893-3
更新日期：2017-04-13 00:00:00
abstract：:Energy storage devices that provide high specific power without compromising on specific energy are highly desirable for many electric-powered applications. Here, we demonstrate that polymer organic radical gel materials support fast bulk-redox charge storage, commensurate to surface double layer ion exchange at carbo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22194
更新日期：2016-02-26 00:00:00
abstract：:The association between blood urea nitrogen to creatinine ratio (UCR) and survival is uncertain in hemodialysis patients. We examined the influence of UCR on mortality and morbidity in hemodialysis patients. A total of 3,401 hemodialysis patients were prospectively followed for 4 years. The association between UCR wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14205-2
更新日期：2017-11-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06622
更新日期：2014-10-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32371
更新日期：2016-09-01 00:00:00
abstract：:The etiology of insulin resistance in Type 1 Diabetes (T1D) is unknown, however it affects approximately 20% of T1D patients. Intramyocellular lipids (IMCL) have been identified as a mechanism of insulin resistance. We examined skeletal muscle of T1D rats to determine if alterations in lipid metabolism were evident an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26379
更新日期：2016-05-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08884
更新日期：2015-03-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32547
更新日期：2016-09-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27470
更新日期：2016-06-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30643-y
更新日期：2018-08-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48461-1
更新日期：2019-08-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54587-z
更新日期：2019-12-04 00:00:00
abstract：:Anaerobic ammonium oxidation (anammox) is known to autotrophically convert ammonium to dinitrogen gas with nitrite as the electron acceptor, but little is known about their released microbial products and how these are relative to heterotrophic growth in anammox system. In this work, we applied a mathematical model to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27576
更新日期：2016-06-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44384
更新日期：2017-03-14 00:00:00
abstract：:Pre-clinical monitoring of tumor growth and identification of distal metastasis requires a balance between accuracy and expediency. Bioluminescence imaging (BLI) is often used to track tumor growth but is primarily limited to planar 2-dimensional (2D) imaging. Consistent subject placement within a standard top-mounted...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21668-4
更新日期：2018-02-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58932-5
更新日期：2020-02-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27455-5
更新日期：2018-06-14 00:00:00
abstract：:It is our contention that for multiscale modelling of infectious disease systems to evolve and expand in scope, it needs to be founded on a theory. Such a theory would improve our ability to describe infectious disease systems in terms of their scales and levels of organization, and their inter-relationships. In this ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52820-3
更新日期：2019-11-08 00:00:00
abstract：:The role of changes in dopamine neuronal activity during the development of symptoms in affective disorders remains controversial. Here, we show that inactivation of NMDA receptors on dopaminergic neurons in adult mice led to the development of affective disorder-like symptoms. The loss of NMDA receptors altered activ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37171
更新日期：2016-11-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42682-0
更新日期：2019-04-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29160-9
更新日期：2018-07-23 00:00:00
