Abstract:
:At the supramolecular level, the proliferation of invasive ductal carcinoma through breast tissue is beyond the range of standard histopathology identification. Using synchrotron small angle x-ray scattering (SAXS) techniques, determining nanometer scale structural changes in breast tissue has been demonstrated to allow discrimination between different tissue types. From a total of 22 patients undergoing symptomatic investigations, different category breast tissue samples were obtained in use of surgically removed tissue, including non-lesional, benign and malignant tumour. Structural components of the tissues were examined at momentum transfer values between q = 0.2 nm-1 and 1.5 nm-1. From the SAXS patterns, axial d-spacing and diffuse scattering intensity were observed to provide the greatest discrimination between the various tissue types, specifically in regard to the epithelial mesenchymal transition (EMT) structural component in malignant tissue. In non-lesional tissue the axial period of collagen is within the range 63.6-63.7 nm (formalin fixed paraffin embedded (FFPE) dewaxed) and 63.4 (formalin fixed), being 0.9 nm smaller than in EMT cancer-invaded regions. The overall intensity of scattering from cancerous regions is a degree of magnitude greater in cancer-invaded regions. Present work has found that the d-spacing of the EMT positive breast cancer tissue (FFPE (dewaxed)) is within the range 64.5-64.7 nm corresponding to the 9th and 10th order peaks. Of particular note in regard to formalin fixation of samples is that no alteration is observed to occur in the relative differences in collagen d-spacing between non-lesional and malignant tissues. This is a matter of great importance given that preserved-sample and also retrospective study of samples is greatly facilitated by formalin fixation. Present results indicate that as aids in tissue diagnosis SAXS is capable of distinguishing areas of invasion by disease as well as delivering further information at the supramolecular level.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mohd Sobri SN,Abdul Sani SF,Sabtu SN,Looi LM,Chiew SF,Pathmanathan D,Chio-Srichan S,Bradley DAdoi
10.1038/s41598-020-58932-5subject
Has Abstractpub_date
2020-02-06 00:00:00pages
1997issue
1issn
2045-2322pii
10.1038/s41598-020-58932-5journal_volume
10pub_type
杂志文章abstract::Atomistic chemical inhomogeneities are anticipated to induce dissimilarities in surface potentials, which control corrosion initiation of alloys at the atomic scale. Precise understanding of corrosion is therefore hampered by lack of definite information describing how atomistic heterogeneities regulate the process. H...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39525
更新日期:2016-12-21 00:00:00
abstract::Maculinea (=Phengaris) are endangered butterflies that are characterized by a very complex biological cycle. Maculinea larvae behave as obligate parasites whose survival is strictly dependent on both particular food plants and species-specific Myrmica ants. In this interaction, Maculinea caterpillars induce Myrmica wo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44514-7
更新日期:2019-05-29 00:00:00
abstract::To assess the metabolically beneficial effects of fenugreek (Trigonella foenum-graecum), C57BL/6J mice were fed a low- or high-fat diet for 16 weeks with or without 2% (w/w) fenugreek supplementation. Body weight, body composition, energy expenditure, food intake, and insulin/glucose tolerance were measured regularly,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12846-x
更新日期:2017-10-06 00:00:00
abstract::Diabetes is expected to accelerate age-related ganglion cell-inner plexiform layer (GC-IPL) loss, but there is limited information on the rate of reduction in GC-IPL thicknesses. We aimed to evaluate the reduction rate of GC-IPL thickness in diabetic patients, and to compare the rates between patients without and with...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58465-x
更新日期:2020-01-30 00:00:00
abstract::We investigate recovery of multispecies oral biofilms following chlorhexidine gluconate (CHX) and CHX with surface modifiers (CHX-Plus) treatment. Specifically, we examine the percentage of viable bacteria in the biofilms following their exposure to CHX and CHX-Plus for 1, 3, and 10 minutes, respectively. Before antim...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27537
更新日期:2016-06-21 00:00:00
abstract::Somatic copy number alterations (SCNAs) affecting oncogenic drivers have a firmly established role in promoting cancer. However, no agreed-upon standard exists for calling locus-specific amplifications and deletions in each patient sample. Here, we report the correlative analysis of copy number amplitude and length wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19649
更新日期:2016-01-20 00:00:00
abstract::The outbreak of multidrug-resistant pathogenic bacteria made the discovery of novel control strategies necessary. Phages have regained attention for their specific lytic activity against pathogenic bacterium. A newly isolated phage infecting the clinical Escherichia coli isolates, including several multidrug-resistant...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26004-4
更新日期:2018-05-24 00:00:00
abstract::Macro-fungi play important roles in the soil elemental cycle in terrestrial ecosystems. Many researchers have focused on the interactions between mycorrhizal fungi and host plants, whilst comparatively few studies aim to characterise the relationships between macro-fungi and bacteria in situ. In this study, we detecte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30120-6
更新日期:2018-08-03 00:00:00
abstract::Mountain glaciers form landscapes with U-shaped valleys, roche moutonées and overdeepenings through bedrock erosion. However, little evidence for active glacial carving has been provided particularly for areas above the Equilibrium Line Altitude (ELA) where glaciers originate. This is mainly due to our lack of informa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43527-6
更新日期:2019-05-06 00:00:00
abstract::It has been recently ascribed to several inflammatory cytokines (i.e. TGF-β3, TNF-α, and IL-1) a functional role in regulating Sertoli cell blood-testis barrier (BTB) dynamics. In the testis, IL-6 inhibits meiotic DNA synthesis during the seminiferous epithelium cycle, reduces sperm motility and influences the secreti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04260
更新日期:2014-03-03 00:00:00
abstract::Reliable P wave detection is necessary for accurate and automatic electrocardiogram (ECG) analysis. Currently, methods for P wave detection in physiological conditions are well-described and efficient. However, methods for P wave detection during pathology are not generally found in the literature, or their performanc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55323-3
更新日期:2019-12-13 00:00:00
abstract::Egg oil from Portunus trituberculatus (Pt-egg oil) can overcome insulin resistance resulting from abundant bioactive lipids. However, its effects on obesity and gut microbiota were unclear. Here, we evaluated whether Pt-egg oil could improve obesity and gut microbiota or not in high-fat diet feeding mice. Results exhi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65199-3
更新日期:2020-05-21 00:00:00
abstract::Drought stress and pathogen infection simultaneously occur in the field. In this study, the interaction of these two stresses with chickpea, their individual and combined effect and the net impact on plant growth and yield traits were systematically assessed under field and confined pot experiments. The field experime...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41463-z
更新日期:2019-04-03 00:00:00
abstract::Herbicides are important constituents of modern integrated weed management system. However, the continuous use of a single herbicide leads to the frequent evolution of resistant weeds which further challenges their management. To overcome this situation, alternating use of multiple herbicides along with conventional w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29554-9
更新日期:2018-08-02 00:00:00
abstract::Increasing life expectancy and a growing share of older people around the world spotlight the issue of health during additional years of life. Research on trends of proportions of older people with activity limitations for low and middle income countries is sparse. We use data from the World Health Survey and the UN W...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67166-4
更新日期:2020-06-26 00:00:00
abstract::We have designed a new type of composite materials which not only has a Young's modulus much larger than the Voigt limit, but also is always nearly isotropic. Moreover, its Poisson's ratio can be designed at a desired value, e.g. positive, or negative, or zero. We have also demonstrated that structural hierarchy can h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14103
更新日期:2015-09-11 00:00:00
abstract::A single bout of electroacupuncture results in muscle contractions and increased whole body glucose uptake in women with polycystic ovary syndrome (PCOS). Women with PCOS have transcriptional and epigenetic alterations in the adipose tissue and we hypothesized that electroacupuncture induces epigenetic and transcripti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17919-5
更新日期:2018-01-30 00:00:00
abstract::This study was to investigate effects of progesterone vaginal sustained-release gel and intramuscular injection of progesterone on frozen-thawed embryos transfer in 3013 patients receiving vaginal progesterone sustained-release gel and progesterone injection in artificial cycle for frozen-thawed embryo transfer. All p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51717-5
更新日期:2019-10-24 00:00:00
abstract::Metal nitrides have been proposed to replace noble metals in plasmonics for some specific applications. In particular, while titanium nitride (TiN) and zirconium nitride (ZrN) possess localized plasmon resonances very similar to gold in magnitude and wavelength, they benefit from a much higher sustainability to temper...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38647
更新日期:2016-12-09 00:00:00
abstract::Red mud as a waste material is produced in large quantities by the aluminum industry. Heat activation has been used to enhance sorption capacity of red mud for its beneficial reuse as an effective sorbent. In this study, heat-activated red mud (HARM) was investigated for its Cd(II) sorption capacity under various proc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31967-5
更新日期:2018-09-10 00:00:00
abstract::Although the Guangxi region accounts for 10% of all HIV-1 cases new reported in 2011 in China, the sources of the transmitted HIV-1 strains are virtually unknown. To determine the extent to which recent HIV infections were derived from already circulating local strains as opposed to recently introduced strains, we per...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29201-3
更新日期:2018-08-27 00:00:00
abstract::Magnetic vortex is one of the simplest topologically non-trivial textures in condensed matter physics. It is the ground state of submicron magnetic elements (dots) of different shapes: cylindrical, square etc. So far, the vast majority of the vortex dynamics studies were focused on thin dots with thickness 5-50 nm and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13881
更新日期:2015-09-10 00:00:00
abstract::The numerous species that make up the oral microbiome are now understood to play a key role in establishment and maintenance of oral health. The ability to taxonomically identify community members at the species level is important to elucidating its diversity and association to health and disease. We report the overal...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep43344
更新日期:2017-02-27 00:00:00
abstract::The accumulation of fat, especially in visceral sites, is a significant risk factor for several chronic diseases with altered cardiometabolic homeostasis. We studied how intensive long-term weight loss and subsequent weight regain affect physiological changes, by longitudinally interrogating the lipid metabolism and w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40107-6
更新日期:2019-03-05 00:00:00
abstract::In East Greenland, a dramatic increase of tuberculosis (TB) incidence has been observed in recent years. Classical genotyping suggests a genetically similar Mycobacterium tuberculosis (Mtb) strain population as cause, however, precise transmission patterns are unclear. We performed whole genome sequencing (WGS) of Mtb...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33180
更新日期:2016-09-12 00:00:00
abstract::Nanocarrier systems for the encapsulation of agrochemicals can contribute to sustainable agriculture, but few nanosystems have been developed for plant growth regulators (PGRs). The present study evaluated the effects of seed priming using alginate/chitosan (nanoALG/CS) and chitosan/tripolyphosphate (nanoCS/TPP) conta...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43494-y
更新日期:2019-05-09 00:00:00
abstract::The relationship between biodiversity and ecological functioning is a central issue in freshwater ecology, but how this relationship is influenced by hydrological connectivity stress is still unknown. In this study we analyzed the dynamic of the phytoplankton alpha diversity indices and their relationships with trophi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78300-7
更新日期:2020-12-07 00:00:00
abstract::As whales recover from commercial exploitation, they are increasing in abundance in habitats that they have been absent from for decades. However, studying the recovery and habitat use patterns of whales, particularly in remote and inaccessible regions, frequently poses logistical and economic challenges. Here we tria...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69887-y
更新日期:2020-07-31 00:00:00
abstract::Epilepsy is a chronic neurological disease with a variable therapeutic response. To design effective treatment strategies for epilepsy, it is important to understand treatment responses and predictive factors. However, limited data are available in Africa, including Ethiopia. The aim of this study was therefore to ass...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52574-y
更新日期:2019-11-07 00:00:00
abstract::Simulation models in respiratory research are increasingly used for medical product development and testing, especially because in-vivo models are coupled with a high degree of complexity and ethical concerns. This work introduces a respiratory simulation system, which is bridging the gap between the complex, real ana...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56176-6
更新日期:2019-12-24 00:00:00