Abstract:
:There is a growing need for low-cost, portable technologies for the detection of threats to the environment and human health. Here we propose a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-phenoxybenzoic acid (3-PBA), a biomarker for monitoring human exposure to synthetic pyrethroid insecticides. The biosensor functions like a competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as the detection element. When the engineered cells are mixed with 3-PBA-protein conjugate crosslinking that can be visually detected occurs. Free 3-PBA in samples competes with these crosslinks, leading to a detectable change in the output. The assay performance was improved by coloring the cells via expression of the purple-blue amilCP chromoprotein and the VHH expression level was reduced to obtain a limit of detection of 3 ng/mL. The optimized biosensor exhibited robust function in complex sample backgrounds such as synthetic urine and plasma. Furthermore, lyophilization enabled storage of biosensor cells for at least 90 days without loss of functionality. Our whole-cell biosensor is simple and low-cost and therefore has potential to be further developed as a screening tool for monitoring exposure to pyrethroids in low-resource environments.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Riangrungroj P,Bever CS,Hammock BD,Polizzi KMdoi
10.1038/s41598-019-48907-6subject
Has Abstractpub_date
2019-08-28 00:00:00pages
12466issue
1issn
2045-2322pii
10.1038/s41598-019-48907-6journal_volume
9pub_type
杂志文章abstract::Although macrophages are important players in the injury/repair processes in animal models of acute kidney injury (AKI), their roles in human AKI remains uncertain owing to a paucity of human biopsy studies. We investigated the role of macrophages in 72 cases of biopsy-proven acute tubular necrosis (ATN) and six cases...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58725-w
更新日期:2020-02-07 00:00:00
abstract::Tumor oxygenation has been correlated with treatment outcome for radiotherapy. In this work, the dependence of tumor oxygenation on tumor vascularity and blood oxygenation was determined quantitatively in a 4D stochastic computational model of head and neck squamous cell carcinoma (HNSCC) tumor growth and angiogenesis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11444-1
更新日期:2017-09-08 00:00:00
abstract::Proper structural organization of spiral ganglion (SG) innervation is crucial for normal hearing function. However, molecular mechanisms underlying the developmental formation of this precise organization remain not well understood. Here, we report in the developing mouse cochlea that deleted in colorectal cancer (Dcc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23799
更新日期:2016-04-04 00:00:00
abstract::Understanding the drivers of animal movement is significant for ecology and biology. Yet researchers have so far been unable to fully understand these drivers, largely due to low data resolution. In this study, we analyse a high-frequency movement dataset for a group of grazing cattle and investigate their spatiotempo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31967
更新日期:2016-08-24 00:00:00
abstract::We demonstrate a comprehensive quantitative analysis of vector beam states (VBSs) by using a vector field reconstruction (VFR) technique integrating interferometry and imaging polarimetry, where the analysis is given by a cylindrically polarized Laguerre-Gaussian (LG) mode expansion of VBSs. From test examples of cyli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46390-7
更新日期:2019-07-10 00:00:00
abstract::Paeonia ostii, a member of tree peony, is an emerging oil crop with important medical and oil uses and widely cultivated in China. Dissolving the genetic diversity and domestication history of this species is important for further genetic improvements and deployments. We firstly selected 29 simple sequence repeats (SS...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04744-z
更新日期:2017-07-13 00:00:00
abstract::Loss of olfactomedin 4 (OLFM4) gene expression is associated with the progression of human prostate cancer, but its role and the molecular mechanisms involved in this process have not been completely understood. In this study, we found that Olfm4-knockout mice developed prostatic intraepithelial neoplasia and prostati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16974
更新日期:2015-11-19 00:00:00
abstract::While the feasibility of transpapillary drug delivery has previously been established, localized transport via the mammary ducts may be improved with tailored drug delivery formulations. The objective of this study was to investigate the impact of nanoemulsion encapsulation on transpapillary delivery in vitro. Nanoemu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48144-x
更新日期:2019-08-14 00:00:00
abstract::Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36448
更新日期:2016-11-02 00:00:00
abstract::PDA is a major cause of US cancer-related deaths. Oncogenic Kras presents in 90% of human PDAs. Kras mutations occur early in pre-neoplastic lesions but are insufficient to cause PDA. Other contributing factors early in disease progression include chronic pancreatitis, alterations in epigenetic regulators, and tumor s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77373-8
更新日期:2020-11-26 00:00:00
abstract::Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41868-w
更新日期:2019-04-02 00:00:00
abstract::Bovine milk is vital for infant nutrition and is a major component of the human diet. Bovine mastitis is a common inflammatory disease of mammary gland in cattle. It alters the immune profile of the animal and lowers the quality and yield of milk causing huge economic losses to dairy industry. The incidence of sub-cli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66211-6
更新日期:2020-06-09 00:00:00
abstract::Cell therapy using intramuscular injections of autologous bone-marrow mononuclear cells (BM-MNCs) improves clinical symptoms and can prevent limb amputation in atherosclerotic peripheral arterial disease (PAD) patients with critical limb ischemia (CLI). The purpose of this study was to evaluate the effects of the numb...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76886-6
更新日期:2020-11-16 00:00:00
abstract::The nanoscale molecular assembly of mammalian viruses during their infectious life cycle remains poorly understood. Their small dimensions, generally bellow the 300nm diffraction limit of light microscopes, has limited most imaging studies to electron microscopy. The recent development of super-resolution (SR) light m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29132
更新日期:2016-07-04 00:00:00
abstract::Basal-like breast cancer (BLBC) is associated with a poor clinical outcome due to the few treatment options and absence of effective targeted agents. Here, we show that malic enzyme 1 (ME1) is dramatically upregulated in BLBC due to ME1 copy number amplification. ME1 expression increases glucose uptake and lactate pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35106-y
更新日期:2018-11-13 00:00:00
abstract::Ionizing radiation (IR)-induced intestinal damage is characterized by a loss of intestinal crypt cells, intestinal barrier disruption and translocation of intestinal microflora resulting in sepsis-mediated lethality. We have shown that mice lacking C/EBPδ display IR-induced intestinal and hematopoietic injury and leth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49437-x
更新日期:2019-09-27 00:00:00
abstract::Several cobalt-based Heusler alloys have been predicted to exhibit Weyl Semimetal behavior due to time reversal symmetry breaking. Co2TiGe is one of the predicted ferromagnetic Weyl semimetals. In this work, we report weak localization and small anomalous Hall conductivity in half-metallic Co2TiGe thin films grown by ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39037-0
更新日期:2019-03-04 00:00:00
abstract::Emergence of multiple drug resistant strains of pathogenic bacteria calls for new initiatives to combat infectious diseases. Gold nanoparticles (AuNPs), because of their non-toxic nature and size/shape dependent optical properties, offer interesting possibility. Here we report the antibacterial efficacy of AuNPs of di...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09357-0
更新日期:2017-08-29 00:00:00
abstract::Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07699
更新日期:2015-01-09 00:00:00
abstract::Despite recent advances in melanoma treatment, metastasis and resistance to therapy remain serious clinical challenges. NME1 is a metastasis suppressor, a class of proteins which inhibits metastatic spread of cancer cells without impact on growth of the primary tumor. We have identified a rare subpopulation of cells w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58996-3
更新日期:2020-02-06 00:00:00
abstract::Osteoporosis medication in fragility fracture patients is associated with better outcomes. However, limited studies have investigated whether fracture types affect outcomes among patients undergoing treatment. We performed a secondary data analysis on participants from a fracture liaison service and an osteoporosis me...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46315-4
更新日期:2019-07-12 00:00:00
abstract::Methylphenidate (MP) is the most prescribed psychostimulant for ADHD patients, with clinically demonstrated detrimental effects on bone quality, potentially leading to early onset osteoporosis and higher fracture risk. The underlying mechanism for the effects of MP on bone remains elusive. This study demonstrates that...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19894-x
更新日期:2018-01-24 00:00:00
abstract::Given the rapidly expanding library of disease biomarkers and targeting agents, the number of unique targeted nanoparticles is growing exponentially. The high variability and expense of animal testing often makes it unfeasible to examine this large number of nanoparticles in vivo. This often leads to the investigation...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05840
更新日期:2014-07-28 00:00:00
abstract::Tropical Pacific variability (TPV) heavily influences global climate, but much is still unknown about its drivers. We examine the impact of South Pacific variability on the modes of TPV: the El Niño-Southern Oscillation (ENSO) and the Interdecadal Pacific Oscillation (IPO). We conduct idealised coupled experiments in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52805-2
更新日期:2019-12-04 00:00:00
abstract::The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in position 2 of the stem peptide plays a decisive role in the polymerization of cell wall building blocks. S. aureus mutants with a reduced degree of amidation are less viable and show increased susceptibility to methicil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31098-x
更新日期:2018-08-28 00:00:00
abstract::We report an observation of rapid (exceeding 2,000 K/s) heating of polydimethylsiloxane (PDMS), one of the most popular microchannel materials, under cyclic loadings at high (~MHz) frequencies. A microheater was developed based on the finding. The heating mechanism utilized vibration damping in PDMS induced by sound w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11851
更新日期:2015-07-03 00:00:00
abstract::Although early diagnosis and treatment reduce the risk of blindness from glaucoma, the decision on whether or not to begin treatment in patients with suspected glaucoma is often a dilemma because the majority of patients never develop definite glaucoma. A growing body of evidences suggests that posterior bowing of the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17843-8
更新日期:2018-01-15 00:00:00
abstract::This paper proposes new ways to assess handwriting, a critical skill in any child's school journey. Traditionally, a pen and paper test called the BHK test (Concise Evaluation Scale for Children's Handwriting) is used to assess children's handwriting in French-speaking countries. Any child with a BHK score above a cer...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60011-8
更新日期:2020-02-21 00:00:00
abstract::Osteopontin (OPN) is involved in cell proliferation, migration, inflammation, and tumor progression in various tissues. OPN induces stemness by interacting with CD44, but the functional relevance of OPN-mediated interferon (IFN) signaling and hepatitis C virus (HCV) replication in stem cell populations remains unclear...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31421-6
更新日期:2018-09-03 00:00:00
abstract::Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 hea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01487-9
更新日期:2017-05-02 00:00:00