Abstract：
:Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Botka T,Pantůček R,Mašlaňová I,Benešík M,Petráš P,Růžičková V,Havlíčková P,Varga M,Žemličková H,Koláčková I,Florianová M,Jakubů V,Karpíšková R,Doškař Jdoi
10.1038/s41598-019-41868-wsubject
Has Abstractpub_date
2019-04-02 00:00:00pages
5475issue
1issn
2045-2322pii
10.1038/s41598-019-41868-wjournal_volume
9pub_type
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