Abstract：
:Lipid droplet (LD)-associated hydrolase (LDAH) is a newly identified LD protein abundantly expressed in tissues that predominantly store triacylglycerol (TAG). However, how LDAH regulates TAG metabolism remains unknown. We found that upon oleic acid loading LDAH translocalizes from the ER to newly formed LDs, and induces LD coalescence in a tubulin-dependent manner. LDAH overexpression and downregulation in HEK293 cells increase and decrease, respectively, TAG levels. Pulse and chase experiments show that LDAH enhances TAG biogenesis, but also decreases TAG turnover and fatty acid release from cells. Mutations in predicted catalytic and acyltransferase motifs do not influence TAG levels, suggesting that the effect is independent of LDAH's enzymatic activity. However, a LDAH alternative-splicing variant missing 90 amino acids at C-terminus does not promote LD fusion or TAG accumulation, while it still localizes to LDs. Interestingly, LDAH enhances polyubiquitination and proteasomal degradation of adipose triglyceride lipase (ATGL), a rate limiting enzyme of TAG hydrolysis. Co-expression of ATGL reverses the changes in LD phenotype induced by LDAH, and both proteins counterbalance their effects on TAG stores. Together, these studies support that under conditions of TAG storage in LDs LDAH plays a primarily lipogenic role, inducing LD growth and enhancing degradation of ATGL.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Goo YH,Son SH,Paul Adoi
10.1038/s41598-017-02963-ysubject
Has Abstractpub_date
2017-06-02 00:00:00pages
2743issue
1issn
2045-2322pii
10.1038/s41598-017-02963-yjournal_volume
7pub_type
杂志文章abstract：:The purpose of the study was to assess both anatomic and functional outcomes between short-pulse continuous wavelength and infrared micropulse lasers in the treatment of DME. This was a prospective interventional study from tertiary care eye hospital-King Khaled Eye Specialist Hospital (Riyadh, Saudi Arabia). Patients...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79699-9
更新日期：2021-01-08 00:00:00
abstract：:Excessive activation of inflammation and the accompanying lung vascular endothelial barrier disruption are primary pathogenic features of acute lung injury (ALI). Microtubule-associated protein 4 (MAP4), a tubulin assembly-promoting protein, is important for maintaining the microtubule (MT) cytoskeleton and cell-cell ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08895
更新日期：2015-03-09 00:00:00
abstract：:Social projection is the tendency to project one's own characteristics onto others. This phenomenon can potentially explain cooperation in prisoner's dilemma experiments and other social dilemmas. The social projection hypothesis has recently been formalized for symmetric games as co-action equilibrium and for general...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-19085-0
更新日期：2018-01-15 00:00:00
abstract：:Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni-Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19742
更新日期：2016-01-28 00:00:00
abstract：:State of the art X-ray Free Electron Laser facilities currently provide the brightest X-ray pulses available, typically with mJ energy and several hundred femtosecond duration. Here we present one- and two-dimensional Particle-in-Cell simulations, utilising the process of stimulated Raman amplification, showing that t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16755
更新日期：2015-11-16 00:00:00
abstract：:Immune checkpoint inhibitors (ICIs) elicit antitumour effects by activating the host immunity and cause immune-related adverse events (irAEs). ICI-related interstitial lung disease (ICI-ILD) is a fatal irAE that is difficult to treat; moreover, its incidence is relatively higher in patients with lung cancer. Therefore...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70743-2
更新日期：2020-08-13 00:00:00
abstract：:The nanoscale molecular assembly of mammalian viruses during their infectious life cycle remains poorly understood. Their small dimensions, generally bellow the 300nm diffraction limit of light microscopes, has limited most imaging studies to electron microscopy. The recent development of super-resolution (SR) light m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29132
更新日期：2016-07-04 00:00:00
abstract：:Many plant essential oils and their terpenoid constituents possess bioactivities including insecticidal activity, and they sometimes act synergistically when mixed. Although several hypotheses for this have been proposed, the underlying mechanism has not been fully elucidated thus far. In the present study, we report ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42432
更新日期：2017-02-09 00:00:00
abstract：:Langerhans cells (LCs) are able to orchestrate adaptive immune responses in the skin by interpreting the microenvironmental context in which they encounter foreign substances, but the regulatory basis for this has not been established. Utilising systems immunology approaches combining in silico modelling of a reconstr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00651-5
更新日期：2017-04-06 00:00:00
abstract：:Basic leucine zippers (bZIPs) form a large plant transcription factor family. C and S1 bZIP groups can heterodimerize, fulfilling crucial roles in seed development and stress response. S1 sequences also harbor a unique regulatory mechanism, termed Sucrose-Induced Repression of Translation (SIRT). The conservation of b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30444
更新日期：2016-07-26 00:00:00
abstract：:Metamaterial absorbers can provide good solutions for radar-cross-section (RCS) reduction. In spite of their attractive features of thinness, lightness, and low cost, resonant metamaterial absorbers have a drawback of narrow bandwidth. For practical radar applications, wideband absorbers are necessary. In this paper, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31823
更新日期：2016-08-22 00:00:00
abstract：:Rubber dams are widely used for landscaping in urban rivers and they retain large amounts of sediments. The sediments are rich in phosphorus (P) which can cause river eutrophication. Little is known about P release in rubber dams. We investigated the potential of sediment P release by isotherm experiment in an urban r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59493-3
更新日期：2020-02-17 00:00:00
abstract：:Optical resolution photoacoustic microscopy (OR-PAM) is a non-invasive, label-free method of in vivo imaging with microscopic resolution and high optical contrast. Based on intrinsic contrasts, OR-PAM has expanded to include in vivo vessel imaging, flow cytometry, physiological parameter analysis, and single-cell char...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13224-3
更新日期：2017-10-17 00:00:00
abstract：:Nasopharyngeal carcinoma (NPC) is a highly metastatic cancer that is consistently associated with Epstein-Barr virus (EBV) infection. In this study, we identify for the first time a role for monoamine oxidase A (MAOA) in NPC. MAOA is a mitochondrial enzyme that catalyzes oxidative deamination of neurotransmitters and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63150-0
更新日期：2020-04-09 00:00:00
abstract：:RNaseIII proteins are dsRNA-specific endonucleases involved in many important biological processes, such as small RNA processing and maturation in eukaryotes. Various small RNAs have been identified in a protozoan parasite Entamoeba histolytica. EhRNaseIII is the only RNaseIII endonuclease domain (RIIID)-containing pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44832
更新日期：2017-03-20 00:00:00
abstract：:Bioactive nanosilicates are emerging prominent next generation biomaterials due to their intrinsic functional properties such as advanced biochemical and biophysical cues. Recent studies show interesting dose-dependent effect of fluoride ions on the stem cells. Despite of interesting properties of fluoride ions as wel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37327-7
更新日期：2019-01-29 00:00:00
abstract：:The molecular bases of the Frank-Starling law of the heart and of its cellular counterpart, the length dependent activation (LDA), are largely unknown. However, the recent discovery of the thick filament activation, a second pathway beside the well-known calcium mediated thin filament activation, is promising for eluc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36706-4
更新日期：2019-02-04 00:00:00
abstract：:Pediatric obstructive sleep-disordered breathing is associated with growth retardation, but also with obesity that has a tendency to persist following treatment. We investigated the effect of upper airways obstruction (AO) and of obstruction removal (OR) in juvenile rats on gut-derived ghrelin and related hypothalamic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02843-5
更新日期：2017-06-02 00:00:00
abstract：:High-throughput technology can now provide rich information on a person's biological makeup and environmental surroundings. Important discoveries have been made by relating these data to various health outcomes in fields such as genomics, proteomics, and medical imaging. However, cross-investigations between several h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36076
更新日期：2016-10-26 00:00:00
abstract：:Mammalian target of rapamycin (mTOR) is a central regulator of cellular metabolism. The importance of mTORC1 signaling in neuronal development and functions has been highlighted by its strong relationship with many neurological and neuropsychiatric diseases. Previous studies demonstrated that hyperactivation of mTORC1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38730-4
更新日期：2019-02-26 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-54657-2
更新日期：2019-11-25 00:00:00
abstract：:We aimed to investigate the diagnostic accuracy of contrast-enhanced ultrasound (CEUS) in evaluating blunt abdominal trauma for patients presenting to the emergency department. Electronic search of Scopus and Pubmed was performed from inception to September 2016. Human studies investigating the diagnostic accuracy of ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-04779-2
更新日期：2017-06-30 00:00:00
abstract：:Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite. In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace pores, suggesting that...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29376
更新日期：2016-07-20 00:00:00
abstract：:Designer nucleases, like zinc-finger nucleases (ZFNs), represent valuable tools for targeted genome editing. Here, we took advantage of the gamma-retroviral life cycle and produced vectors to transfer ZFNs in the form of protein, mRNA and episomal DNA. Transfer efficacy and ZFN activity were assessed in quantitative p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04656
更新日期：2014-04-11 00:00:00
abstract：:The effect of hypoglycemia on the progression of atherosclerosis in patients with type 2 diabetes mellitus (T2DM) remains largely unknown. This is a post hoc analysis of a randomized trial to investigate the relationship between hypoglycemic episodes and changes in carotid intima-media thickness (IMT). Among 274 study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39965
更新日期：2017-01-09 00:00:00
abstract：:We report a method to generate a 3D motor neuron model with segregated and directed axonal outgrowth. iPSC-derived motor neurons are cultured in extracellular matrix gel in a microfluidic platform. Neurons extend their axons into an adjacent layer of gel, whereas dendrites and soma remain predominantly in the somal co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81335-z
更新日期：2021-01-22 00:00:00
abstract：:The nucleotides guanosine tetraphosphate and pentaphosphate (together known as (p)ppGpp or magic spot) are produced in plant plastids from GDP/GTP and ATP by RelA-SpoT homologue (RSH) enzymes. In the model plant Arabidopsis (p)ppGpp regulates chloroplast transcription and translation to affect growth, and is also impl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54207-w
更新日期：2019-11-27 00:00:00
abstract：:Triboelectric charging is common in desert sandstorms and dust devils on Earth; however, it remains poorly understood. Here we show a charging mechanism of sands with the adsorbed water on micro-porous surface in wind-blown sand based on the fact that water content is universal but usually a minor component in most pa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01337
更新日期：2013-01-01 00:00:00
abstract：:There is a huge request for the development of low dielectric constant polymeric materials for microelectronic applications. In thisstudy, polymer blends based on PVA:POZ with low dielectric constant has been fabricated. The results of XRD indicate that crystalline domain is enhanced at higher POZ concentration. Brill...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49715-8
更新日期：2019-09-11 00:00:00
abstract：:Motility dysfunction is present not only during bowel obstruction (BO), but after obstruction is resolved. Previous studies found that lumen distension associated mechano-transcription of COX-2 and production of PGE2 in gut smooth muscle cells (SMC) account for motility dysfunction during obstruction. We hypothesized ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27230-6
更新日期：2018-06-11 00:00:00
