Abstract：
:Injectable hydrogels based on extracellular matrix-derived polymers show much promise in the field of tissue engineering and regenerative medicine. However, the hydrogels reported to date have at least one characteristic that limits their potential for clinical use, such as excessive swelling, complicated and potentially toxic cross-linking process, or lack of shear thinning and self-healing properties. We hypothesized that a collagen hydrogel cross-linked using thiol-Michael addition click reaction would be able to overcome these limitations. To this end, collagen was modified to introduce thiol groups, and hydrogels were prepared by cross-linking with 8-arm polyethylene glycol-maleimide. Rheological measurements on the hydrogels revealed excellent shear-thinning and self-healing properties. Additionally, only minimal swelling (6%) was observed over a period of 1 month in an aqueous buffer solution. Finally, tests using mesenchymal stromal cells and endothelial cells showed that the hydrogels are cell-compatible and suitable for cell encapsulation and delivery. Thus, the reported thiolated-collagen hydrogel cross-linked using thiol-Michael addition click reaction overcomes most of the challenges in the injectable hydrogel design and is an excellent candidate for cell delivery in regenerative medicine and tissue engineering applications. The hydrogel reported here is the first example of a self-healing hydrogel containing covalent cross-links.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Pupkaite J,Rosenquist J,Hilborn J,Samanta Adoi
10.1021/acs.biomac.9b00769subject
Has Abstractpub_date
2019-09-09 00:00:00pages
3475-3484issue
9eissn
1525-7797issn
1526-4602journal_volume
20pub_type
杂志文章abstract：:Polymer conjugation increases an enzyme's circulation time and stability for use as a therapeutic agent, but this attachment indubitably affects its properties. Covalent attachment of multiple polyethylene glycol chains with sizes of either 2, 5, 10, or 20 kDa increases the molecular weight and hydrodynamic radius of ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1006954
更新日期：2010-12-13 00:00:00
abstract：:Receptor-mediated, cell-specific delivery of siRNA enables silencing of target genes in specific tissues, opening the door to powerful therapeutic options for a multitude of diseases. However, the development of delivery systems capable of targeted and effective siRNA delivery typically requires multiple steps and the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200485b
更新日期：2011-07-11 00:00:00
abstract：:The conformational behavior of a long single-chain double-stranded DNA in solutions of free silver ions and silver nanoparticles generated via the reduction of AgNO3 by NaBH4 was monitored by fluorescence and electron microscopies and UV spectroscopy. The interaction of monovalent silver ions with DNA induces shrinkin...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800235j
更新日期：2008-07-01 00:00:00
abstract：:Hybrid agarose hydrogels embedded with pH-responsive diblock copolymers micelles were developed to achieve functional hydrogels capable of stimulus-triggered drug release. Specifically, a well-defined poly(ethylene oxide) (PEO)-based diblock copolymer, PEO-b-poly(2-(N,N-diisopropylamino)ethyl methacrylate) (PEO(113)-b...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4005639
更新日期：2013-08-12 00:00:00
abstract：:The gold standard for implant metals is titanium, and coatings such as collagen-I, RGD-peptide, chondroitin sulfate, and calcium phosphate have been used to modify its biocompatibility. We investigated how titanium coated with pectins, adaptable bioactive plant polysaccharides with anti-inflammatory effects, supports ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800356b
更新日期：2008-09-01 00:00:00
abstract：:This study clarifies the contribution to overall osmotic kinetics of colloid osmotic pressure (Pi) and the interaction of synthetic colloids with the membrane. Solutions (6%) of dextran with weight average molecular weight (MW(w)) 68 800 (DEX 70), dextran with MW(w) 40 000 (DEX 40), hydroxyethyl starch with MW(w) 70 0...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0500143
更新日期：2005-05-01 00:00:00
abstract：:Synthetic and natural mucoadhesive biomaterials in optimized galenical formulations are potentially useful for the transmucosal delivery of active ingredients to improve their localized and prolonged effects. Chitosans (CS) have potent mucoadhesive characteristics, but the exact mechanisms underpinning such interactio...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5007954
更新日期：2014-10-13 00:00:00
abstract：:As a programmable biopolymer, DNA has shown great potential in the fabrication and construction of nanometer-scale assemblies and devices. In this report, we described a strategy for efficient manipulation of gold nanoparticle-bound DNA using restriction endonuclease. The digestion efficiency of this restriction enzym...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060517o
更新日期：2006-11-01 00:00:00
abstract：:Oriented immobilization of proteins is an important step in creating protein-based functional materials. In this study, a method was developed to orient proteins on hydroxyapatite (HA) surfaces, a widely used bone implant material, to improve protein bioactivity by employing enhanced green fluorescent protein (EGFP) a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201865r
更新日期：2012-06-11 00:00:00
abstract：:By monitoring the thermally driven displacements of imbedded polystyrene microspheres via video fluorescence microscopy, we quantified the microstructural and micromechanical heterogeneities of wheat gliadin suspensions. We found that the degree of heterogeneity of the suspensions, as measured by the width and skewnes...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015586b
更新日期：2002-01-01 00:00:00
abstract：:Plastics ending up in soil or landfill environment will eventually be degraded. At the same time a slow migration of additives from the plastics will occur. The additives and the degradation products of these and the polymer are potential compounds for bioconversion. To predict the rate of the migration of antioxidant...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0000283
更新日期：2000-10-01 00:00:00
abstract：:The leading cause of death in the United States is cardiovascular disease. The majority of these cases result from heart failure post-myocardial infarction (MI). We present data providing evidence for use of acetalated dextran (AcDex) microparticles as a delivery vehicle for therapeutics to the heart post-MI. We harne...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401050j
更新日期：2013-11-11 00:00:00
abstract：:Bovine hemoglobin (Hb) was encapsulated inside polymer vesicles (polymersomes) to form polymersome encapsulated Hb (PEH) dispersions. PEH particles are 100% surface PEGylated with longer PEG chains and possess thicker hydrophobic membranes as compared to conventional liposomes. Polymersomes were self-assembled from po...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0501454
更新日期：2005-07-01 00:00:00
abstract：:A new 320-member polymer library of end-modified poly(β-amino ester)s was synthesized. This library was chosen such that small differences to the structures of component backbone, side-chain, and end-group monomers could be systematically and simultaneously evaluated. The in vitro transfection efficacy and cytotoxicit...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200807s
更新日期：2011-10-10 00:00:00
abstract：:The electrospinning of polymer melts can offer an advantage over solution electrospinning, in the development of layered tissue constructs for tissue engineering. Melt electrospinning does not require a solvent, of which many are cytotoxic in nature, and the use of nonwater soluble polymers allows the collection of fi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050777q
更新日期：2006-03-01 00:00:00
abstract：:Fibrin sealants are a type of soft tissue adhesive that employs biochemical reactions from the late stages of the blood coagulation cascade. Intrinsic to these adhesives are a structural protein and a transglutaminase crosslinking enzyme. We are investigating an alternative biomimetic adhesive based on gelatin and a c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034529a
更新日期：2004-07-01 00:00:00
abstract：:The growing importance of biodegradable conducting polymers (CPs) have fueled the rapid development of this unique class of polymeric materials in recent years. Possessing both the electrical conductivity approaching those of metallic conductors and the biodegradability of biocompatible polymers, biodegradable CPs are...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.8b00275
更新日期：2018-06-11 00:00:00
abstract：:Reversible addition-fragmentation chain transfer (RAFT) radical polymerization, mediated by 4-cyanopentanoic acid dithiobenzoate and 4,4'-azobis(4-cyanovaleric acid) (V-501) in water at 70 degrees C, of biocompatible 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) yields a macro-chain transfer agent (CTA) that was em...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8014945
更新日期：2009-04-13 00:00:00
abstract：:Owing to their diverse functions and tunable physicochemical properties, peptides are promising alternatives to the conventional gene delivery tools that are available for plant systems. However, peptide-mediated gene delivery is limited by low transfection efficiency in plants because of the insufficient cytosolic tr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00481
更新日期：2020-07-13 00:00:00
abstract：:Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modificati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00470
更新日期：2015-07-13 00:00:00
abstract：:meso-Tetra(N-methyl-4-pyridyl) porphine tetra tosylate (TMP) is a photosensitizer that can be used in photodynamic therapy (PDT) to induce cell death through generation of reactive oxygen species in targeted tumor cells. However, TMP is highly hydrophilic, and therefore, its ability to accumulate intracellularly is li...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301858a
更新日期：2013-02-11 00:00:00
abstract：:A novel aptamer modified thermosensitive liposome was designed as an efficient magnetic resonance imaging probe. In this paper, Gd-DTPA was encapsulated into an optimized thermosensitive liposome (TSL) formulation, followed by conjugation with AS1411 for specific targeting against tumor cells that overexpress nucleoli...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00250
更新日期：2015-09-14 00:00:00
abstract：:First generation, photocrosslinkable dendrimers consisting of natural metabolites (i.e., succinic acid, glycerol, and beta-alanine) and nonimmunogenic poly(ethylene glycol) (PEG) were synthesized divergently in high yields using ester and carbamate forming reactions. Aqueous solutions of these dendrimers were photocro...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800658x
更新日期：2008-10-01 00:00:00
abstract：:Chitosan fibers showing narrow diameter distribution with a mean of 42 nm were produced by electrospinning and utilized for the sorption of Fe(III), Cu(II), Ag(I), and Cd(II) ions from aqueous solutions. The ion concentrations in the supernatant solutions were determined using inductively coupled plasma-mass spectrome...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100755x
更新日期：2010-12-13 00:00:00
abstract：:The dynamics of bacterial colonies is complex in nature because it correlates the behavior of numerous individual cells in space and time and is characterized by emergent properties such as virulence or antibiotics resistance. Because there is no clear-cut evidence that periodic swarming of P. mirabilis colonies is ru...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm061181l
更新日期：2007-04-01 00:00:00
abstract：:Chemo-photodynamic combined therapy is promising in cancer treatment, although low tissue penetration of visible light for activating photosensitizers (e.g., chlorin e6, Ce6) limited its broad applications. Combination of upcoverting nanoparticles (UCNPs) with the photosensitizers endows us with the possibility to uti...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01211
更新日期：2019-10-14 00:00:00
abstract：:Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm070062i
更新日期：2007-09-01 00:00:00
abstract：:G-quadruplex is a type of DNA secondary structure formed by specific guanine-rich sequences. Because of their enrichment in functional genomic regions and their biological significance, G-quadruplexes are recognized as significant drug targets for cancer and other diseases. Here, we tested the precipitation efficiency...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100862k
更新日期：2010-12-13 00:00:00
abstract：:We report the facile preparation of gels from the hydrothermal treatment of suspensions of cellulose nanocrystals (CNCs). The properties of the hydrogels have been investigated by rheology, electron microscopy, and spectroscopy with respect to variation in the temperature, time, and CNC concentration used in preparati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00906
更新日期：2016-08-08 00:00:00
abstract：:Because of their association with debilitating diseases and their potential applications in developing novel bionanomaterials, highly ordered amyloid fibrils have recently received considerable attention. While many studies have thus far focused on amyloid fibrils made with short peptides containing just one steric zi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00218
更新日期：2019-05-13 00:00:00
