Abstract：
:Mesenchymal stromal cells (MSC) are used in cell therapies, however cellular senescence increases heterogeneity of cell populations and leads to uncertainty in therapies' outcomes. The determination of cellular senescence is time consuming and logistically intensive. Here, we propose the use of endogenous autofluorescence as real-time quantification of cellular senescence in human MSC, based on label-free flow cytometry analysis. We correlated cell autofluorescence to senescence using senescence-associated beta-galactosidase assay (SA-β-Gal) with chromogenic (X-GAL) and fluorescent (C12FDG) substrates, gene expression of senescence markers (such as p16INK4A, p18INK4C, CCND2 and CDCA7) and telomere length. Autofluorescence was further correlated to MSC differentiation assays (adipogenesis, chondrogenesis and osteogenesis), MSC stemness markers (CD90/CD106) and cytokine secretion (IL-6 and MCP-1). Increased cell autofluorescence significantly correlated with increased SA-β-Gal signal (both X-GAL and C12FDG substrates), cell volume and cell granularity, IL-6/MCP-1 secretion and with increased p16INK4A and CCND2 gene expression. Increased cell autofluorescence was negatively associated with the expression of the CD90/CD106 markers, osteogenic and chondrogenic differentiation potentials and p18INK4C and CDCA7 gene expression. Cell autofluorescence correlated neither with telomere length nor with adipogenic differentiation potential. We conclude that autofluorescence can be used as fast and non-invasive senescence assay for comparing MSC populations under controlled culture conditions.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Bertolo A,Baur M,Guerrero J,Pötzel T,Stoyanov Jdoi
10.1038/s41598-019-38546-2subject
Has Abstractpub_date
2019-02-14 00:00:00pages
2074issue
1issn
2045-2322pii
10.1038/s41598-019-38546-2journal_volume
9pub_type
杂志文章abstract：:Baited remote underwater video cameras were deployed in the Eastern Canadian Arctic, for the purpose of estimating local densities of the long-lived Greenland shark within five deep-water, data-poor regions of interest for fisheries development and marine conservation in Nunavut, Canada. A total of 31 camera deploymen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-19115-x
更新日期：2018-01-17 00:00:00
abstract：:Magnetic skyrmions are hailed as a potential technology for data storage and other data processing devices. However, their stability against thermal fluctuations is an open question that must be answered before skyrmion-based devices can be designed. In this work, we study paths in the energy landscape via which the t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03391-8
更新日期：2017-06-22 00:00:00
abstract：:Ethiopian mustard (Brassica carinata A. Braun) is an emerging sustainable source of vegetable oil, in particular for the biofuel industry. The present study exploited genome assemblies of the Brassica diploids, Brassica nigra and Brassica oleracea, to discover over 10,000 genome-wide SNPs using genotype by sequencing ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69255-w
更新日期：2020-07-28 00:00:00
abstract：:This study demonstrated the usefulness of radiomic features based on the Hessian index of differential topology for the prediction of prognosis prior to treatment in head-and-neck (HN) cancer patients. The Hessian index, which can indicate tumor heterogeneity with convex, concave, and other points (saddle points), was...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78338-7
更新日期：2020-12-04 00:00:00
abstract：:This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy-Weinberg equilibrium, n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81425-y
更新日期：2021-01-21 00:00:00
abstract：:The Hauraki Gulf, a shallow embayment in north-eastern New Zealand, provides an interesting environment for ecological soundscape research. It is situated on a tectonic plate boundary, contains one of the busiest ports in the southern hemisphere and is home to a diverse range of soniferous animals. The underwater soun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06347-0
更新日期：2017-07-18 00:00:00
abstract：:Chromosome changes are one of the hallmarks of human malignancies. Chromosomal rearrangement is frequent in human cancers. One of the consequences of chromosomal rearrangement is gene fusions in the cancer genome. We have previously identified a panel of fusion genes in aggressive prostate cancers. In this study, we s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38550-6
更新日期：2019-01-31 00:00:00
abstract：:Pseudomonas aeruginosa is a highly invasive human pathogen in spite of the absence of classical host specific virulence factors. Virulence factors regulated by quorum sensing (QS) in P. aeruginosa cause acute infections to shift to chronic diseases. Several small regulatory RNAs (sRNAs) mediate fine-tuning of bacteria...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36488-9
更新日期：2019-01-23 00:00:00
abstract：:The DEAD-box RNA helicase DDX3 plays divergent roles in tumorigenesis, however, its function in mitosis is unclear. Immunofluorescence indicated that DDX3 localized to centrosome throughout the cell cycle and colocalized with centrosome-associated p53 during mitosis in HCT116 and U2OS cells. DDX3 depletion promoted ch...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09779-w
更新日期：2017-08-25 00:00:00
abstract：:Here we provide, at least to our knowledge, the first evidence that aripiprazole (APPZ) in the water blunts the stress response of exposed fish in a concentration ten times lower than the concentration detected in the environment. Although the mechanism of APPZ in the neuroendocrine axis is not yet determined, our res...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37612
更新日期：2016-11-22 00:00:00
abstract：:Bacterial β-glucuronidase (GUS) enzymes cause drug toxicity by reversing Phase II glucuronidation in the gastrointestinal tract. While many human gut microbial GUS enzymes have been examined with model glucuronide substrates like p-nitrophenol-β-D-glucuronide (pNPG), the GUS orthologs that are most efficient at proces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36069-w
更新日期：2019-01-29 00:00:00
abstract：:Cassava is an important staple crop in tropical and sub-tropical areas. As a common farming practice, cassava is usually cultivated intercropping with other crops and subjected to various degrees of shading, which causes reduced productivity. Herein, a comparative transcriptomic analysis was performed on a series of d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31673
更新日期：2016-08-19 00:00:00
abstract：:The FGFR4-388Arg variant has been related to poor prognosis in several types of cancer, including lung cancer. The mechanism underlying this association has not been addressed in detail in patients with this pathology. Here, we report that this FGFR4 variant induces MAPK and STAT3 activation and causes pro-oncogenic e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20570-3
更新日期：2018-02-05 00:00:00
abstract：:15-lipoxygenase is involved in the generation of specialized pro-resolving lipid mediators that play essential roles in resolution and inflammatory responses. Here, we investigated anti-inflammatory role of Alox15 in skin homeostasis. We demonstrated that knockout (KO) of Alox15 led to hair loss and disrupted the stru...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27221-7
更新日期：2018-06-11 00:00:00
abstract：:To investigate the safety and efficiency of using robotic staplers for intracorporeal gastroduodenostomy in reduced-port robotic gastrectomy for gastric adenocarcinoma. We retrospectively reviewed patients who underwent totally robotic and laparoscopic gastrectomy with intracorporeal gastroduodenostomy. Gastroduodenos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71807-z
更新日期：2020-09-07 00:00:00
abstract：:We demonstrate numerically and experimentally the enhancement of Surface Plasmon Resonance (SPR) sensing via dispersion engineering of the plasmonic response using plasmonic nanograting. Following their design and optimization, the plasmonic nanograting structures are fabricated using e-beam lithography and lift-off p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27023-x
更新日期：2018-06-13 00:00:00
abstract：:The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and to the accumulation of lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation of translational activity with a decrease in cell size, proliferat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42005-3
更新日期：2019-04-04 00:00:00
abstract：:The intensification of marine aquaculture raises multiple sustainability issues, namely the handling of nutrient-rich effluents that can adversely impact ecosystems. As integrated multi-trophic aquaculture (IMTA) gains momentum, the use of halophyte plants to phytoremediate aquaculture effluents has received growing a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66093-8
更新日期：2020-06-22 00:00:00
abstract：:The development of efficient green light-emitting diodes (LEDs) is of paramount importance for the realization of colour-mixing white LEDs with a high luminous efficiency. While the insertion of an InGaN/GaN superlattice (SL) with a lower In content before the growth of InGaN/GaN multiple quantum wells (MQWs) is known...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29440-4
更新日期：2018-07-23 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-017-15019-y
更新日期：2017-11-10 00:00:00
abstract：:It has been shown that long-term stimulant consumption alters the biological and microbiological status of the oral cavity. We present a pilot study describing stimulant-specific oral immunomodulation in the oral cavity. Changes in salivary cytokine levels in response to long-term alcohol, tobacco and caffeine were id...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35094-z
更新日期：2018-11-12 00:00:00
abstract：:Active immunotherapy targeting β-amyloid (Aβ) is the most promising strategy to prevent or treat Alzheimer's disease (AD). Based on pre-clinical studies and clinical trials, a safe and effective AD vaccine requires a delicate balance between providing therapeutically adequate anti-Aβ antibodies and eliminating or supp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07771
更新日期：2015-01-14 00:00:00
abstract：:Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities of thermophilic Geobacillus s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72866-y
更新日期：2020-10-12 00:00:00
abstract：:Traditionally, dynamic atomic force microscopy (AFM) techniques are based on the analysis of the quasi-steady state response of the cantilever deflection in terms of Fourier analysis. Here we describe a technique that instead exploits the often disregarded transient response of the cantilever through a relatively mode...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49104-1
更新日期：2019-09-03 00:00:00
abstract：:This study aimed to investigate the predictive efficacy of positron emission tomography/computed tomography (PET/CT) and magnetic resonance imaging (MRI) for the pathological response of advanced breast cancer to neoadjuvant chemotherapy (NAC). The breast PET/MRI image deep learning model was introduced and compared w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77875-5
更新日期：2020-12-03 00:00:00
abstract：:Maximal rate of heart rate (HR) increase (rHRI) as a measure of HR acceleration during the transition from rest to exercise, or during an increase in workload, tracks exercise performance. rHRI assessed at relative rather than absolute workloads may track performance better, and a field test would increase applicabili...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71597-4
更新日期：2020-09-03 00:00:00
abstract：:Magnetite (Fe3O4) is an iron ore mineral that is globally mined especially for steel production. It is denser (5.15 g/cm3) than Earth's crust (~2.7 g/cm3) and is expected to accumulate at the bottom of melt-rich magma reservoirs. However, recent studies revealed heterogeneous fluid bubble nucleation on oxide minerals ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40376-1
更新日期：2019-03-07 00:00:00
abstract：:This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake). We used an in vitro human colonic microbiota model based on batch fermentation s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18877-8
更新日期：2018-01-11 00:00:00
abstract：:Systemic lupus erythematosus (SLE) patients are vulnerable to infections. We aim to explore the approach to differentiate active infection from disease activity in pediatric SLE patients. Fifty pediatric SLE patients presenting with 185 clinical visits were collected. The associations between both clinical and laborat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76789-6
更新日期：2020-11-16 00:00:00
abstract：:Pre-operative discrimination of malignant masses is crucial for accurate diagnosis and prompt referral to a gynae oncology centre for optimal surgical intervention. HGSOC progression is correlated with local and systemic inflammation. We hypothesised that inclusion of inflammatory biomarkers in sera may improve diagno...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59009-z
更新日期：2020-02-10 00:00:00
