Abstract：
:The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on the reaction velocity of the popular GOx-HRP cascade, particularly in the presence of a competing enzyme (catalase). Herein, we rationalize these experimental results quantitatively. We show that, in general, proximity channeling can enhance reaction velocity in the presence of competing enzymes, but in steady state a significant enhancement can only be achieved for diffusion-limited reactions or at high concentrations of competing enzymes. We provide simple equations to estimate the effect of channeling quantitatively and demonstrate that proximity can have a more pronounced effect under crowding conditions in vivo, particularly that crowding can enhance the overall rates of channeled cascade reactions.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kuzmak A,Carmali S,von Lieres E,Russell AJ,Kondrat Sdoi
10.1038/s41598-018-37034-3subject
Has Abstractpub_date
2019-01-24 00:00:00pages
455issue
1issn
2045-2322pii
10.1038/s41598-018-37034-3journal_volume
9pub_type
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