Abstract：
:Neutrophils are the first immune cells to kill invading microbes at sites of infection using a variety of processes, including the release of proteases, phagocytosis and the production of neutrophil extracellular traps (NETs). NET formation, or NETosis, is a specific and highly efficient process, which is induced by a variety of stimuli leading to expulsion of DNA, proteases and antimicrobial peptides to the extracellular space. However, uncontrolled NETosis may lead to adverse effects and exert tissue damage in pathological conditions. Here, we show that the ATP channel pannexin1 (Panx1) is functionally expressed by bone marrow-derived neutrophils (BMDNs) of wild-type (WT) mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate (PMA). Interestingly, neutrophils isolated from Panx1-/- mice showed reduced and/or delayed induction of NETosis. Brilliant blue FCF dye (BB-FCF), a Panx1 channel inhibitor, decreased NETosis in wild-type neutrophils to the extent observed in Panx1-/- neutrophils. Thus, we demonstrate that ATP and Panx1 channels contribute to NETosis and may represent a therapeutic target.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sofoluwe A,Bacchetta M,Badaoui M,Kwak BR,Chanson Mdoi
10.1038/s41598-019-53058-9subject
Has Abstractpub_date
2019-11-12 00:00:00pages
16556issue
1issn
2045-2322pii
10.1038/s41598-019-53058-9journal_volume
9pub_type
杂志文章abstract：:Affinity maturation is one of the cardinal strategies for improving antibody function using in vitro evolutionary methods; one such well-established method is phage display. To minimise gene deletion, we previously developed an open sandwich (OS) method wherein selection was performed using only phage-displaying VH fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23796-3
更新日期：2018-04-03 00:00:00
abstract：:We developed hybrid slot antenna structures for microbial sensing in the THz frequency range, where silver nanowires (AgNWs) were employed to increase the sensitivity. In order to fabricate the hybrid devices, we partially etched the AgNW in the slot antenna region, where we can expect the field enhancement effect at ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33617-2
更新日期：2018-10-19 00:00:00
abstract：:We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13289
更新日期：2015-08-21 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-33559-9
更新日期：2018-10-23 00:00:00
abstract：:The simplest and most commonly used acoustic levitator is comprised of a transmitter and an opposing reflecting surface. This type of device, however, is only able to levitate objects along one direction, at distances multiple of half of a wavelength. In this work, we show how a customised reflective acoustic metamate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60978-4
更新日期：2020-03-06 00:00:00
abstract：:In the present study rice straw (R, control) was mixed with Cowdung (C), Azolla (A) and cellulolytic fungus Aspergillus terreus (F) in different combinations viz. RC, RA, RF, RCF, RCA, RFA and RCFA and subjected to aerobic composting (Acom) and vermicomposting (Vcom - with Eisenia fetida). It was found that addition o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37880-1
更新日期：2019-02-04 00:00:00
abstract：:There is no treatment for the myelin loss in multiple sclerosis, ultimately resulting in the axonal degeneration that leads to the progressive phase of the disease. We established a multi-tiered platform for the sequential screening of drugs that could be repurposed as remyelinating agents. We screened a library of 2,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45780
更新日期：2017-04-07 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41598-017-18707-x
更新日期：2018-01-15 00:00:00
abstract：:Global lake systems have undergone rapid degradation over the past century. Scientists and managers are struggling to manage the highly degraded lake systems to cope with escalating anthropogenic pressures. Improved knowledge of how lakes and social systems co-evolved up to the present is vital for understanding, mode...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35482-5
更新日期：2018-11-21 00:00:00
abstract：:Lung cancer is the deadliest cancer worldwide. The mutational frequency of EGFR and KRAS genes in lung adenocarcinoma varies worldwide per ethnicity and smoking. The impact of EGFR and KRAS mutations in Brazilian lung cancer remains poorly explored. Thus, we investigated the frequency of EGFR and KRAS mutations in a l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39965-x
更新日期：2019-03-01 00:00:00
abstract：:Multiferroic BiFeO3 exhibits excellent magnetoelectric coupling critical for magnetic information processing with minimal power consumption. However, the degenerate nature of the easy spin axis in the (111) plane presents roadblocks for real world applications. Here, we explore the stabilization and switchability of t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12969
更新日期：2015-08-06 00:00:00
abstract：:Nucleosome not only directly affects cellular processes, such as DNA replication, recombination, and transcription, but also severs as a fundamentally important target of epigenetic modifications. Our previous study indicated that the bending property of DNA is important in nucleosome formation, particularly in predic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27247-x
更新日期：2018-06-11 00:00:00
abstract：:Most Group I Clostridium botulinum strains harbor botulinum neurotoxin (bont) genes on their chromosome, while some carry these genes (including bont/a, bont/b, and bont/f) on large plasmids. Prior work in our laboratory demonstrated that Group I BoNT plasmids were mobilized to C. botulinum recipient strains containin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21342-9
更新日期：2018-02-15 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-42889-1
更新日期：2019-06-10 00:00:00
abstract：:Identification of nodal metastasis and tumor extranodal extension (ENE) is crucial for head and neck cancer management, but currently only can be diagnosed via postoperative pathology. Pretreatment, radiographic identification of ENE, in particular, has proven extremely difficult for clinicians, but would be greatly i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32441-y
更新日期：2018-09-19 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide. Most of studies have focused on molecular and pathogenic characterization of PEDV, whereas there were limited studies in understanding the role of gut microbiota (GM) in viral-associated diarrhea. Here, using the Illumina MiSe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17830-z
更新日期：2017-12-12 00:00:00
abstract：:If a cultural feature is transmitted over generations and exposed to stochastic selection when spreading in a population, its evolution may be governed by statistical laws and be partly predictable, as in the case of genetic evolution. Music exhibits steady changes of styles over time, with new characteristics develop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52380-6
更新日期：2019-11-05 00:00:00
abstract：:Oil palm (Elaeis guinensis Jacquin) is the most important source of vegetable oil and fat. Several linkage maps had been constructed using dominant and co-dominant markers to facilitate mapping of QTL. However, dominant markers are not easily transferable among different laboratories. We constructed a consensus linkag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08232
更新日期：2015-02-04 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep23458. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/srep46934
更新日期：2018-01-08 00:00:00
abstract：:The overlap between pain and reward processing pathways leds researchers to hypothesize that there are interactions between them in the human brain. Two hypotheses have been proposed. The "competition hypothesis" posits that reward can reduce pain-related neural activity and vice versa. The "salience hypothesis" sugge...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26426
更新日期：2016-05-19 00:00:00
abstract：:Partner of sld five 1 (PSF1) is an evolutionary conserved DNA replication factor involved in DNA replication in lower species, which is strongly expressed in normal stem cell populations and progenitor cell populations. Recently, we have investigated PSF1 functions in cancer cells and found that PSF1 plays a significa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11605-2
更新日期：2017-09-11 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-54946-w
更新日期：2019-12-02 00:00:00
abstract：:The cortisol stress response and the molecular programming of the corticoid axis were characterized for the first time during early ontogeny in a Mediterranean marine teleost, the European sea bass (Dicentrarchus labrax). Sea bass embryos, pre-larvae and larvae at specific points of development were exposed to acute s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05525
更新日期：2014-07-02 00:00:00
abstract：:This work provides new insights into the dynamics of silica nanoparticle-based removal of organic fluids (here oil) from naturally occurring porous media. We have used 4D (time-resolved 3D) imaging at pore-scale using X-ray computed micro-tomography (μCT) technique. The captured 3D tomographic time-series data reveal ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29569-2
更新日期：2018-07-24 00:00:00
abstract：:Antimicrobial peptides (AMPs) are peptide antibiotics with a broad spectrum of antimicrobial activities. Activity prediction of AMPs from their amino acid sequences is of great therapeutic importance but imposes challenges on prediction methods due to label interactions. In this paper we propose a novel multi-label le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01986-9
更新日期：2017-05-19 00:00:00
abstract：:Birds have a highly specialized and efficient digestive system, but when this system originated remains uncertain. Here we report six gastric pellets attributable to the recently discovered 160-million-year-old troodontid dinosaur Anchiornis, which is among the key taxa for understanding the transition to birds. The g...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32202-x
更新日期：2018-09-21 00:00:00
abstract：:The Killari Earthquake (Moment magnitude 6.1) of September 30, 1993, occurred in the state of Maharashtra, India, has an epicenter (18°03' N, 76°33' E) located at ~ 40 km SSW of Killari Town. The ~ 125 km long basin of Tirna River, close to the Killari Town, currently occupies the area that has witnessed episodic intr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77542-9
更新日期：2020-11-25 00:00:00
abstract：:Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery. As human pluripotent stem cell-derived cardiomyocytes remain functionally fetal-type, close monitoring of electrophysiological maturation is critical for their furthe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43210
更新日期：2017-03-07 00:00:00
abstract：:Employing theranostic nanoparticles, which combine both therapeutic and diagnostic capabilities in one dose, has promise to propel the biomedical field toward personalized medicine. Here we investigate the theranostic properties of topological insulator bismuth selenide (Bi2Se3) in in vivo and in vitro system for the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01998
更新日期：2013-01-01 00:00:00
abstract：:During anaerobic digestion (AD) process, process parameters e.g., VFA, pH, COD removal … and kinetic parameters e.g., hydrolysis rate, lag phase and methane production potential… are the important indicator for illustrating AD process performance, however, the AD process performance based on these parameters remains p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09977-6
更新日期：2017-08-24 00:00:00
