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High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules.

Abstract：

:Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally.

journal_name

Nat Commun

journal_title

Nature communications

authors

Heath GR,Scheuring S

doi

10.1038/s41467-018-07512-3

subject

Has Abstract

pub_date

2018-11-26 00:00:00

pages

4983

issue

1

issn

2041-1723

pii

10.1038/s41467-018-07512-3

journal_volume

9

pub_type

杂志文章

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