Transcription factor YcjW controls the emergency H2S production in E. coli.

Abstract:

:Prokaryotes and eukaryotes alike endogenously generate the gaseous molecule hydrogen sulfide (H2S). Bacterial H2S acts as a cytoprotectant against antibiotics-induced stress and promotes redox homeostasis. In E. coli, endogenous H2S production is primarily dependent on 3-mercaptopyruvate sulfurtransferase (3MST), encoded by mstA. Here, we show that cells lacking 3MST acquire a phenotypic suppressor mutation resulting in compensatory H2S production and tolerance to antibiotics and oxidative stress. Using whole genome sequencing, we identified a non-synonymous mutation within an uncharacterized LacI-type transcription factor, ycjW. We then mapped regulatory targets of YcjW and discovered it controls the expression of carbohydrate metabolic genes and thiosulfate sulfurtransferase PspE. Induction of pspE expression in the suppressor strain provides an alternative mechanism for H2S biosynthesis. Our results reveal a complex interaction between carbohydrate metabolism and H2S production in bacteria and the role, a hitherto uncharacterized transcription factor, YcjW, plays in linking the two.

journal_name

Nat Commun

journal_title

Nature communications

authors

Luhachack L,Rasouly A,Shamovsky I,Nudler E

doi

10.1038/s41467-019-10785-x

subject

Has Abstract

pub_date

2019-06-28 00:00:00

pages

2868

issue

1

issn

2041-1723

pii

10.1038/s41467-019-10785-x

journal_volume

10

pub_type

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