Abstract：
:Proteolipid protein 2 (PLP2), a membrane protein of the endoplasmic reticulum, is related to tumor proliferation and metastasis in some human cancers, but not in gliomas. First, we performed western-blot analysis, real-time quantitative PCR and immunohistochemical stains to detect PLP2 expression in 4 glioma cell lines and human glioma tissues. In addition, we used small interfering RNA (SiPLP2) and short hairpin RNA (shPLP2) to knockdown PLP2 expression in GBM8401 and LN229 glioma cell lines. After then, the alteration of PLP2 suppressed glioma cells behavior were examined by cell proliferation, wound healing, cell invasion, and colonies formation assays. Finally, the possible mechanism of PLP2 was analyzed by detecting the expression of the proteins related to cell-cycle checkpoints, cell-proliferative signaling factors, and cell-matrix interaction. Compared with normal brain cell lysates and mRNA, all glioma cell lines displayed PLP2 protein and mRNA overexpression. Besides, higher PLP2 IHC staining significantly correlated with more advanced tumor grades and poorer prognosis in human gliomas. Both siPLP2 transfected gliomas showed a clear inhibition of glioma cell proliferation, migration, and invasion as well as down-regulating p-p38, p-ERK, MMP-2, and MMP-9 expression. In conclusion, we successfully demonstrated that PLP2 overexpression played an oncogenic role in glioma development and aggressive tumor behavior.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Chen YH,Hueng DY,Tsai WCdoi
10.3390/ijms19113353subject
Has Abstractpub_date
2018-10-26 00:00:00issue
11issn
1422-0067pii
ijms19113353journal_volume
19pub_type
杂志文章abstract：:Fractalkine/CX3C chemokine ligand 1 (CX3CL1) is a chemokine involved in the anticancer function of lymphocytes-mainly NK cells, T cells and dendritic cells. Its increased levels in tumors improve the prognosis for cancer patients, although it is also associated with a poorer prognosis in some types of cancers, such as...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21103723
更新日期：2020-05-25 00:00:00
abstract：:Over the past few decades, metal nanoparticles less than 100 nm in diameter have made a substantial impact across diverse biomedical applications, such as diagnostic and medical devices, for personalized healthcare practice. In particular, silver nanoparticles (AgNPs) have great potential in a broad range of applicati...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2019-02-17 00:00:00
abstract：:Previously, we have found that mildronate [3-(2,2,2-trimethylhydrazinium) propionate dihydrate], a small molecule with charged nitrogen and oxygen atoms, protects mitochondrial metabolism that is altered by inhibitors of complex I and has neuroprotective effects in an azidothymidine-neurotoxicity mouse model. In the p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11114465
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abstract：:Recently, neuromediators such as substance P (SP) have been found to be important factors in tendon homeostasis. Some studies have found SP to be the cause of inflammation and tendinopathy, whereas others have determined it to be a critical component of tendon healing. As demonstrated by these conflicting findings, th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21228633
更新日期：2020-11-16 00:00:00
abstract：:Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous system (CNS) tumors. Overall, 96 human serum samples were obtained...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-07-28 00:00:00
abstract：:Abstract: Cancer heterogeneity and progression are subject to complex interactions between neoplastic cells and their microenvironment, including the immune system. Although glioblastomas (GBMs) are classified as 'cold tumours' with very little lymphocyte infiltration, they can contain up to 30-40% of tumour-associate...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-01-21 00:00:00
abstract：:The present editorial aims to summarize the 17 scientific papers that have contributed to this Special Issue focusing on different aspects of basic and translational research in colorectal cancer. ...
journal_title：International journal of molecular sciences
pub_type： 社论
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更新日期：2019-06-25 00:00:00
abstract：:Sericin is a major constituent of silk produced by silkworms. We previously found that the instillation of sericin enhanced the proliferation of corneal epithelial cells, and acted to promote corneal wound healing in both normal and diabetic model rats. However, the mechanisms by which sericin promotes the proliferati...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041123
更新日期：2018-04-09 00:00:00
abstract：:Much of the experimental data, especially in life sciences, is considered to be useless if it demonstrates a large standard deviation from the mean value. The Renaissance distribution, as presented in this study, allows one to extract true values from such statistical data with large noise. To obtain proof of the Rena...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20133250
更新日期：2019-07-02 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13033022
更新日期：2012-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17091393
更新日期：2016-08-26 00:00:00
abstract：:The interaction between programmed cell death protein (PD-1) and its ligand (PD-L1) is one of the main pathways used by some tumors to escape the immune response. In recent years, immunotherapies based on the use of antibodies against PD-1/PD-L1 have been postulated as a great promise for cancer treatment, increasing ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2019-05-09 00:00:00
abstract：:Pea (Pisum sativum L.) is a model plant that has been used in classical genetics and organ development studies. However, its large and complex genome has hindered research investigations in pea. Here, we generated transcriptomes from different tissues or organs of three pea accessions using next-generation sequencing ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18122710
更新日期：2017-12-20 00:00:00
abstract：:Neurodegenerative proteinopathies are complex diseases that share some pathogenetic processes. One of these is the failure of the proteostasis network (PN), which includes all components involved in the synthesis, folding, and degradation of proteins, thus leading to the aberrant accumulation of toxic protein aggregat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176405
更新日期：2020-09-03 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19051390
更新日期：2018-05-07 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20112774
更新日期：2019-06-06 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160716216
更新日期：2015-07-17 00:00:00
abstract：:Peripheral blood stem cells from healthy donors mobilized by granulocyte colony-stimulating factor (G-CSF) and harvested by leukapheresis are commonly used for allogeneic stem cell transplantation. The frequency of severe graft versus host disease is similar for patients receiving peripheral blood and bone marrow allo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17071158
更新日期：2016-07-19 00:00:00
abstract：:Many nutrients are absorbed via Na+ cotransport systems, and therefore it is predicted that nutrient absorption mechanisms require a large amount of luminal Na+. It is thought that Na+ diffuses back into the lumen via paracellular pathways to support Na+ cotransport absorption. However, direct experimental evidence in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020376
更新日期：2020-01-07 00:00:00
abstract：:A distinct G-protein coupled receptor that senses changes in extracellular Zn2+, ZnR/GPR39, was found in cells from tissues in which Zn2+ plays a physiological role. Most prominently, ZnR/GPR39 activity was described in prostate cancer, skin keratinocytes, and colon epithelial cells, where zinc is essential for cell g...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2018-02-01 00:00:00
abstract：:The tumor targetability and stimulus responsivity of drug delivery systems are crucial in cancer diagnosis and treatment. In this study, hepatoma-targeting mixed micelles composed of a hyaluronic acid-glycyrrhetinic acid conjugate and a hyaluronic acid-l-histidine conjugate (HA-GA/HA-His) were prepared through ultraso...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17040364
更新日期：2016-03-30 00:00:00
abstract：:A correlation between gastrointestinal (GI) inflammation and gut hormones has reported that inflammatory stimuli including bacterial endotoxins, lipopolysaccharides (LPS), TNFα, IL-1β, and IL-6 induces high levels of incretin hormone leading to glucose dysregulation. Although incretin hormones are immediately secreted...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20102537
更新日期：2019-05-23 00:00:00
abstract：:In the elderly, whole-body health largely relies on healthy skeletal muscle, which controls body stability, locomotion, and metabolic homeostasis. Age-related skeletal muscle structural/functional deterioration is associated with a higher risk of severe comorbid conditions and poorer outcomes, demanding major socioeco...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21031010
更新日期：2020-02-04 00:00:00
abstract：:Short-term abiotic stress treatment before harvest can enhance the quality of horticultural crops cultivated in controlled environments. Here, we investigated the effects of combined chilling and UV-A treatment on the accumulation of phenolic compounds in kale (Brassica oleracea var. acephala). Five-week-old plants we...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20194950
更新日期：2019-10-08 00:00:00
abstract：:Oenothein B is a unique macrocyclic ellagitannin dimer that has been found in various medicinal plants belonging to Onagraceae, Lythraceae, and Myrtaceae, with diverse biological activities. The immunological effects of tannins in terms of cytokine-release from macrophages and monocytes have been discussed, while the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14010046
更新日期：2012-12-20 00:00:00
abstract：:When Clostridium tetani was discovered and identified as a Gram-positive anaerobic bacterium of the genus Clostridium, the possibility of turning its toxin into a valuable biological carrier to ameliorate neurodegenerative processes was inconceivable. However, the non-toxic carboxy-terminal fragment of the tetanus tox...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms13066883
更新日期：2012-01-01 00:00:00
abstract：:Eosinophilic colitis (EC) is a common cause of haematochezia in infants and young children. The exact pathomechanism is not understood, and the diagnosis is challenging. The role of microRNAs as key class of regulators of mRNA expression and translation in patients with EC has not been explored. Therefore, the aim of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051050
更新日期：2017-05-12 00:00:00
abstract：:Maize ZmC1 and ZmR transcription factors belong to the MYB-type and bHLH families, respectively, and control anthocyanin biosynthesis. In this study, Agrobacterium-mediated transformation was used to generate transgenic wheat plants that overexpress ZmC1 and ZmR or both, with the objective of developing anthocyanin-en...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20225806
更新日期：2019-11-19 00:00:00
abstract：:The application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation. In the present study, we measured the effects of supplying 5 mM nitrate on the growth of nodules, primary root, and lateral roots under light at 28 °C or dark at 18 °...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15034464
更新日期：2014-03-13 00:00:00
abstract：:The circadian transcriptional network is based on a competition between transcriptional activator and repressor complexes regulating the rhythmic expression of clock-controlled genes. We show here that the MYC-associated factor X, MAX, plays a repressive role in this network and operates through a MYC-independent bind...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072294
更新日期：2020-03-26 00:00:00
