Abstract:
:Eosinophilic colitis (EC) is a common cause of haematochezia in infants and young children. The exact pathomechanism is not understood, and the diagnosis is challenging. The role of microRNAs as key class of regulators of mRNA expression and translation in patients with EC has not been explored. Therefore, the aim of the present study was to explore the miRNA profile in EC with respect to eosinophilic inflammation. Patients enrolled in the study (n = 10) had persistent rectal bleeding, and did not respond to elimination dietary treatment. High-throughput microRNA sequencing was carried out on colonic biopsy specimens of children with EC (EC: n = 4) and controls (C: n = 4) as a preliminary screening of the miRNA profile. Based on the next-generation sequencing (NGS) results and literature data, a potentially relevant panel of miRNAs were selected for further measurements by real-time reverse transcription (RT)-PCR (EC: n = 14, C: n = 10). Validation by RT-PCR resulted in significantly altered expression of miR-21, -31, -99b, -125a, -146a, -184, -221, -223, and -559 compared to controls (p ≤ 0.05). Elevation in miR-21, -99b, -146a, -221, and -223 showed statistically significant correlation to the extent of tissue eosinophilia. Based on our results, we conclude that the dysregulated miRNAs have a potential role in the regulation of apoptosis by targeting Protein kinase B/Mechanistic target of rapamycin (AKT/mTOR)-related pathways in inflammation by modulating Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-related signalling and eosinophil cell recruitment and activation, mainly by regulating the expression of the chemoattractant eotaxin and the adhesion molecule CD44. Our results could serve as a basis for further extended research exploring the pathomechanism of EC.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kiss Z,Béres NJ,Sziksz E,Tél B,Borka K,Arató A,Szabó AJ,Veres Gdoi
10.3390/ijms18051050subject
Has Abstractpub_date
2017-05-12 00:00:00issue
5issn
1422-0067pii
ijms18051050journal_volume
18pub_type
杂志文章abstract::Bile acids are a family of amphipathic compounds predominantly known for their role in solubilizing and absorbing hydrophobic compounds (including liposoluble vitamins) in the intestine. Bile acids also are key signaling molecules and inflammatory agents that activate transcriptional factors and cell signaling pathway...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20081869
更新日期:2019-04-16 00:00:00
abstract::RNA binding protein (RBPs) and microRNAs (miRNAs or miRs) are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer ce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16047112
更新日期:2015-03-30 00:00:00
abstract::Platycodin D (PD), a major saponin (platycoside) in Platycodi radix (balloon flower root), has higher pharmacological activity than the other major platycosides; however, its content in the plant root is only approximately 10% (w/w) and the productivities of PD by several enzymes are still too low for industrial appli...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20163854
更新日期:2019-08-07 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs with a known role as mediators of gene expression in crucial biological processes, which converts them into high potential contenders in the ongoing search for effective therapeutic strategies. However, extracellular RNAs are unstable and rapidly degraded, reducing the poss...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031105
更新日期:2021-01-22 00:00:00
abstract::The Gram-negative Campylobacter jejuni is a major cause of foodborne gastroenteritis in humans worldwide. The cytotoxic effects of Campylobacter have been mainly ascribed to the actions of the cytolethal distending toxin (CDT): it is mandatory to put in evidence risk factors for sequela development, such as reactive a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062207
更新日期:2020-03-23 00:00:00
abstract::Plants undergo several but very precise molecular, physiological, and biochemical modulations in response to biotic stresses. Mitogen-activated protein kinase (MAPK) cascades orchestrate multiple cellular processes including plant growth and development as well as plant responses against abiotic and biotic stresses. H...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123023
更新日期:2019-06-20 00:00:00
abstract::The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160510016
更新日期:2015-05-04 00:00:00
abstract::Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein splicing mechanism of class 3 inteins by determining crystal struc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218367
更新日期:2020-11-07 00:00:00
abstract::2-Bromopropane (2-BP) is used as an alternative to ozone-depleting cleaning solvents. Previously, we reported that 2-BP has cytotoxic effects on mouse blastocysts and is associated with defects in subsequent development. In the present work, we show that 2-BP induces apoptosis in the inner cell mass of mouse blastocys...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12084991
更新日期:2011-01-01 00:00:00
abstract::The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active prot...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051635
更新日期:2020-02-27 00:00:00
abstract::Ethanol abuse is a common issue in individuals with sedentary lifestyles, unbalanced diets, and metabolic syndrome. Both ethanol abuse and metabolic syndrome have negative impacts on the central nervous system, with effects including cognitive impairment and brain oxidative status deterioration. The combined effects o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020807
更新日期:2021-01-15 00:00:00
abstract::Among serotonin receptors, the 5-HT6 subtype is the most controversial and the least known in the field of molecular mechanisms. The 5-HT6R ligands can be pivotal for innovative treatment of cognitive impairment, but none has reached pharmacological market, predominantly, due to insufficient "druglikeness" properties....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20143420
更新日期:2019-07-12 00:00:00
abstract::Congenital dyserythropoietic anemia type II (CDA II) is a hypo-productive anemia defined by ineffective erythropoiesis through maturation arrest of erythroid precursors. CDA II is an autosomal recessive disorder due to loss-of-function mutations in SEC23B. Currently, management of patients with CDA II is based on tran...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21155577
更新日期:2020-08-04 00:00:00
abstract::Lysin-motif receptor-like kinase PsK1 is involved in symbiosis initiation and the maintenance of infection thread (IT) growth and bacterial release in pea. We verified PsK1 specificity in relation to the Nod factor structure using k1 and rhizobial mutants. Inoculation with nodO and nodE nodO mutants significantly redu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20071624
更新日期:2019-04-01 00:00:00
abstract::Neurodegenerative diseases are characterized by adverse cellular environments and pathological alterations causing neurodegeneration in distinct brain regions. This development is triggered or facilitated by conditions such as hypoxia, ischemia or inflammation and is associated with disruptions of fundamental cellular...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020582
更新日期:2021-01-08 00:00:00
abstract::There is a growing body of evidence showing the importance of physical activity against acute ischemic events in various organs. Ischemia/reperfusion injury (I/R) is characterized by tissue damage as a result of restriction and subsequent restoration of blood supply to an organ. Oxidative stress due to increased react...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21249382
更新日期:2020-12-09 00:00:00
abstract::Histone deacetylases (HDACs) play important roles in transcriptional regulation in eukaryotic cells. Class I deacetylase HDAC1/2 often associates with repressor complexes, such as Sin3 (Switch Independent 3), NuRD (Nucleosome remodeling and deacetylase) and CoREST (Corepressor of RE1 silencing transcription factor) co...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228460
更新日期:2020-11-11 00:00:00
abstract::Postmenopausal females display a chronic inflammatory phenotype with higher levels of circulating pro-inflammatory cytokines. Furthermore, the inflammatory response to injury may be altered under estrogen-deficiency, because it was shown previously that estrogen-deficient mice displayed increased levels of the inflamm...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072070
更新日期:2018-07-16 00:00:00
abstract::Sulforaphane (SFN) is a phytocompound belonging to the isothiocyanate family. Although it was also found in seeds and mature plants, SFN is mainly present in sprouts of many cruciferous vegetables, including cabbage, broccoli, cauliflower, and Brussels sprouts. SFN is produced by the conversion of glucoraphanin throug...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228637
更新日期:2020-11-16 00:00:00
abstract::Wound healing is a multistep process with four overlapping but distinct stages: hemostasis, inflammation, proliferation, and remodeling. An alteration at any stage may lead to the development of chronic non-healing wounds or excessive scar formation. Impaired wound healing presents a significant health and economic bu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19103217
更新日期:2018-10-18 00:00:00
abstract::The biofilm component poly-N-acetylglucosamine (PNAG) is an important virulence determinant in medical-device-related infections caused by ESKAPE group pathogens including Gram-positive Staphylococcus aureus and Gram-negative Acinetobacter baumannii. PNAG presentation on bacterial cell surfaces and its accessibility f...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072465
更新日期:2020-04-02 00:00:00
abstract::Point mutations in genes encoding isoforms of skeletal muscle tropomyosin may cause nemaline myopathy, cap myopathy (Cap), congenital fiber-type disproportion (CFTD), and distal arthrogryposis. The molecular mechanisms of muscle dysfunction in these diseases remain unclear. We studied the effect of the E173A, R90P, E1...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123975
更新日期:2018-12-10 00:00:00
abstract::In the scenario of personalized medicine, targeted therapies are currently the focus of cancer drug development. These drugs can block the growth and spread of tumor cells by interfering with key molecules involved in malignancy, such as receptor tyrosine kinases (RTKs). MET and Recepteur d'Origine Nantais (RON), whic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030722
更新日期:2020-01-22 00:00:00
abstract::The relationships among 55 wheat accessions (47 accessions collected from Iran and eight accessions provided by the Institute of Plant Biology of the University of Zurich, Switzerland) belonging to eight species carrying A genome (Triticum monococcum L., T. boeoticum Boiss., T. urartu Tumanian ex Gandilyan, T. durum D...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11114309
更新日期:2010-11-02 00:00:00
abstract::Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18122565
更新日期:2017-11-29 00:00:00
abstract::Leishmania is a protozoan parasite that causes a wide range of different clinical manifestations in mammalian hosts. It is a major public health risk on different continents and represents one of the most important neglected diseases. Due to the high toxicity of the drugs currently used, and in the light of increasing...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17081270
更新日期:2016-08-19 00:00:00
abstract::Prostate cancer is widely observed to be biologically heterogeneous. Its heterogeneity is manifested histologically as multifocal prostate cancer, which is observed more frequently than unifocal prostate cancer. The clinical and prognostic significance of either focal cancer type is not fully established. To investiga...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140611816
更新日期:2013-06-03 00:00:00
abstract::Yeonsan Ogye is a rare Korean domestic chicken breed whose entire body, including feathers and skin, has a unique black coloring. Although some protein-coding genes related to this unique feature have been examined, non-coding elements have not been widely investigated. Thus, we evaluated coding and non-coding transcr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082359
更新日期:2018-08-10 00:00:00
abstract::This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150915244
更新日期:2014-08-28 00:00:00
abstract::Among popular crops, plum (Prunus domestica L.) has received special attention due to its health-promoting properties. The seasonality of this fruit makes it impossible to consume it throughout the year, so new products in a powder form may offer an alternative to fresh consumption and may be used as high-quality natu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18010176
更新日期:2017-01-17 00:00:00