Abstract：
:Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9) have greatly enabled and accelerated genome editing. By targeting double-strand breaks to user-defined locations, the rates of DNA repair events are greatly enhanced relative to un-catalyzed events at the same sites. However, the underlying biology of each genome-editing nuclease influences the targeting potential, the spectrum of off-target cleavages, the ease-of-use, and the types of recombination events at targeted double-strand breaks. No single genome-editing nuclease is optimized for all possible applications. Here, we focus on the diversity of nuclease domains available for genome editing, highlighting biochemical properties and the potential applications that are best suited to each domain.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Guha TK,Edgell DRdoi
10.3390/ijms18122565subject
Has Abstractpub_date
2017-11-29 00:00:00issue
12issn
1422-0067pii
ijms18122565journal_volume
18pub_type
杂志文章,评审abstract：:The increasing prevalence of inflammatory diseases and the adverse effects associated with the long-term use of current anti-inflammatory therapies prompt the identification of alternative approaches to reestablish immune balance. Apigenin, an abundant dietary flavonoid, is emerging as a potential regulator of inflamm...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17030323
更新日期：2016-03-01 00:00:00
abstract：:Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect CTCs mutations whi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124364
更新日期：2020-06-19 00:00:00
abstract：:Redox active selenium (Se) compounds have gained substantial attention in the last decade as potential cancer therapeutic agents. Several Se compounds have shown high selectivity and sensitivity against malignant cells. The cytotoxic effects are exerted by their biologically active metabolites, with methylselenol (CH₃...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102849
更新日期：2018-09-20 00:00:00
abstract：:Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14023395
更新日期：2013-02-06 00:00:00
abstract：:Nanoscale secondary ion mass spectrometry (NanoSIMS) combined with transmission electron microscopy (TEM) can be a powerful approach to visualize the exact distribution of drugs at the sub-cellular level. In this work, we exploit this approach to identify the distribution and localisation of the organometallic rutheni...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18091869
更新日期：2017-08-29 00:00:00
abstract：:The interaction between the pituitary hormone, adrenocorticotropin (ACTH), and melanocortin-2 receptor (MC2R) orthologs involves the H6 F7 R8 W9 and R/K15 K16 R17 R18 motifs in ACTH making contact with corresponding contact sites on MC2R. Earlier studies have localized the common HFRW binding site of all melanocortin ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174166
更新日期：2019-08-26 00:00:00
abstract：:Adlay (Coix larchryma-jobi L.) was the commonly used Traditional Chinese Medicine (TCM) with high content of seed storage protein. The hydrolyzed bioactive oligopeptides of adlay have been proven to be anti-hypertensive effective components. However, the structures and anti-hypertensive mechanism of bioactive oligopep...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122099
更新日期：2016-12-14 00:00:00
abstract：:This study investigated the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-modified glass ionomer cement (GIC) on shear bond strength (SBS) and remineralisation of artificial "caries-affected" dentine. Human dentine slices were demineralised and allocated to three groups: group 1, conventional G...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081723
更新日期：2017-08-07 00:00:00
abstract：:Growth factors and other agents that could potentially enhance tissue regeneration have been identified, but their therapeutic value in clinical medicine has been limited for reasons such as difficulty to maintain bioactivity of locally applied therapeutics in the protease-rich environment of regenerating tissues. Alt...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161023556
更新日期：2015-09-30 00:00:00
abstract：:Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and M...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11125323
更新日期：2010-01-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most widespread diagnosed cause of dementia in the elderly. It is a progressive neurodegenerative disease that causes memory loss as well as other detrimental symptoms that are ultimately fatal. Due to the urgent nature of this disease, and the current lack of success in treatment and p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010332
更新日期：2020-01-03 00:00:00
abstract：:Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Guiyan1) and Cd-tolerant (cv. Yunyan2) tobacco cultivars to study the ameliorative effects of exogenous glycinebetaine (GB) upon 5 μM Cd stress. The foliar spray of GB markedly reduced Cd concentrations in plants and alleviated Cd-induced soil pl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071612
更新日期：2019-03-31 00:00:00
abstract：:Lung squamous cell carcinoma (LUSC) has a poor prognosis, in part due to poor therapeutic response and limited therapeutic alternatives. Lichens are symbiotic organisms, producing a variety of substances with multiple biological activities. (+)-Usnic acid, an important biologically active metabolite of lichens, has be...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21030876
更新日期：2020-01-29 00:00:00
abstract：:Obesity is an important aspect of the metabolic syndrome and is often associated with chronic inflammation. In this context, inflammation of organs participating in energy homeostasis (such as liver, adipose tissue, muscle and pancreas) leads to the recruitment and activation of macrophages, which secrete pro-inflamma...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010330
更新日期：2020-12-30 00:00:00
abstract：:Paired box gene 3 (Pax3) and cAMP responsive element-binding protein (CREB) directly interact with the cis-acting elements on the promoter of microphthalmia-associated transcription factor isoform M (MITF-M) for transcriptional activation in the melanogenic process. Tyrosinase (Tyro) is a target gene of MITF-M, and fu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249631
更新日期：2020-12-17 00:00:00
abstract：:Small interfering RNA (siRNA) has been recognized as a powerful gene-silencing tool. For therapeutic application, chemical modification is often required to improve the properties of siRNA, including its nuclease resistance, activity, off-target effects, and tissue distribution. Careful siRNA guide strand selection in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155218
更新日期：2020-07-23 00:00:00
abstract：:Autoimmunity and hematological malignancies are often concomitant in patients. A causal bidirectional relationship exists between them. Loss of immunological tolerance with inappropriate activation of the immune system, likely due to environmental and genetic factors, can represent a breeding ground for the appearance...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103139
更新日期：2018-10-12 00:00:00
abstract：:Osteochondrosis is a failure of the endochondral ossification that affects developing joints in humans and several animal species. It is a localized idiopathic joint disorder characterized by focal chondronecrosis and growing cartilage retention, which can lead to the formation of fissures, subchondral bone cysts, or ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246179
更新日期：2019-12-07 00:00:00
abstract：:Inherited bone marrow failure syndromes (IBMFS) are a group of cancer-prone genetic diseases characterized by hypocellular bone marrow with impairment in one or more hematopoietic lineages. The pathogenesis of IBMFS involves mutations in several genes which encode for proteins involved in DNA repair, telomere biology ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21134672
更新日期：2020-06-30 00:00:00
abstract：:Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene that belongs to the ELOVL family and regulates the synthesis of very-long-chain fatty acids (VLCFAs) and sphingolipids, from yeast to mammals. Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell metabolism...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160716440
更新日期：2015-07-20 00:00:00
abstract：BACKGROUND:Left atrial appendage closure (LAAC) represents the interventional alternative to oral anticoagulation for stroke prevention in atrial fibrillation (AF). The metabolism of acylcarnitines was shown to affect cardiovascular diseases. This study evaluates the influence of successful LAAC on the metabolism of ac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020500
更新日期：2018-02-07 00:00:00
abstract：:The development of atherosclerosis is a multi-step process, at least in part controlled by the vascular endothelium function. Observations in humans and experimental models of atherosclerosis have identified monocyte recruitment as an early event in atherogenesis. Chronic inflammation is associated with ageing and its...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140612696
更新日期：2013-06-17 00:00:00
abstract：:Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20122866
更新日期：2019-06-12 00:00:00
abstract：:Aronia melanocarpa (Michx.) Ell. belongs to the Rosaceae family. The purpose of this study is to explore the gastroprotective effect of the Aronia melanocarpa hydro-alcoholic extract (AMHAE) against ethanol-induced gastric ulcer in a rat model. Different concentrations (50, 100, and 200 mg/kg) of AMHAE, or 30 mg/kg of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061195
更新日期：2017-06-05 00:00:00
abstract：:Nuclear β-dystroglycan (β-DG) is involved in the maintenance of nuclear architecture and function. Nonetheless, its relevance in defined nuclear processes remains to be determined. In this study we generated a C2C12 cell-based DG-null model using CRISPR-Cas9 technology to provide insights into the role of β-DG on nucl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21144961
更新日期：2020-07-14 00:00:00
abstract：:The purpose of this study was to investigate the inhibitory activities of ethanolic extracts from Antrodia cinnamomea (EEAC) on lung cancer. Cell proliferation and cell cycle distribution were analyzed using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and flow cytometry, respectively. Wo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030791
更新日期：2018-03-09 00:00:00
abstract：:A review of the data on the modulatory action of adenosine 5'-triphosphate (ATP), the main co-transmitter with acetylcholine, and adenosine, the final ATP metabolite in the synaptic cleft, on neuromuscular transmission is presented. The effects of these endogenous modulators on pre- and post-synaptic processes are dis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176423
更新日期：2020-09-03 00:00:00
abstract：:This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BS...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140714185
更新日期：2013-07-09 00:00:00
abstract：:Studies, including ours, have shown that pro-oxidative stressors, such as chemotherapeutic agents, generate oxidized lipids with agonistic platelet-activating factor (PAF) activity. Importantly, recent reports have implicated that these PAF-agonists are transported extracellularly via microvesicle particles (MVPs). Wh...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010032
更新日期：2018-12-21 00:00:00
abstract：:Fingertip response to trauma represents a fascinating example of tissue regeneration. Regeneration derives from proliferative mesenchymal cells (blastema) that subsequently differentiate into soft and skeletal tissues. Clinically, conservative treatment of the amputated fingertip under occlusive dressing can shift the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051054
更新日期：2017-05-13 00:00:00
