Abstract：
:The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active proteins in clinical settings is not practical as it is fragile under various conditions. In this study, lysozyme was integrated into chitosan nanoparticles (CS-NPs) by the ionic gelation technique to obtain lysozyme immobilized chitosan nanoparticles (Lys-CS-NPs) and then characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM), which showed a small particle size (243.1 ± 2.1 nm) and positive zeta potential (22.8 ± 0.2 mV). The immobilization significantly enhanced the thermal stability and reusability of lysozyme. In addition, compared with free lysozyme, Lys-CS-NPs exhibited superb antibacterial properties according to the results of killing kinetics in vitro and measurement of the minimum inhibitory concentration (MIC) of CS-NPs and Lys-CS-NPs against Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae), Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus). These results suggest that the integration of lysozyme into CS-NPs will create opportunities for the further potential applications of lysozyme as an anti-bacterium agent.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wang Y,Li S,Jin M,Han Q,Liu S,Chen X,Han Ydoi
10.3390/ijms21051635subject
Has Abstractpub_date
2020-02-27 00:00:00issue
5issn
1422-0067pii
ijms21051635journal_volume
21pub_type
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-06-21 00:00:00
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更新日期：2019-04-05 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,随机对照试验
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更新日期：2020-09-15 00:00:00
