Abstract:
:The ability to bind Congo red (Crb+) is associated with virulence of Shigella flexneri and is encoded by a large, 220-kilobase plasmid. We cloned fragments of this plasmid to isolate the sequences encoding Congo red binding, to determine the degree of conservation of these sequences among S. flexneri strains, and to study the molecular basis for loss of the Crb+ phenotype. At least two separate BamHI fragments cloned into plasmid vectors encode Congo red binding in E. coli or S. flexneri. One Crb+ clone, pTKS2, contains a copy of IS1 adjacent to the crb sequences. IS1 appears to be responsible for deletions leading to loss of Congo red binding in this clone. In addition, this clone was found to integrate into the chromosome at relatively high frequency. Integration resulted in loss of the Crb+ phenotype. A second clone, pTKS15, which has only limited homology to pTKS2, also encodes Congo red binding. The Crb+ phenotype of transformants carrying pTKS15 was detected at 37 degrees C but not at 30 degrees C, and thus it resembles Congo red binding in wild-type S. flexneri. HindIII digests of plasmid DNA from 10 different S. flexneri strains were hybridized to both of these Crb+ clones and to an IS1 probe. More than one fragment hybridized to pTKS2 or pTKS15. In general, the sizes of these fragments were the same in S. flexneri strains of different serotypes, indicating conservation of these sequences. Three of five copies of IS1 were also found on the large S. flexneri plasmids. Two of the copies were on fragments of the same size in each strain. Analysis of Crb- derivatives of the 10 strains indicated that, although IS1 may be closely linked to crb sequences on the 220-kilobase plasmid, it is not responsible for the majority of deletions of this plasmid associated with loss of Congo red binding.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Daskaleros PA,Payne SMdoi
10.1128/IAI.54.2.435-443.1986subject
Has Abstractpub_date
1986-11-01 00:00:00pages
435-43issue
2eissn
0019-9567issn
1098-5522journal_volume
54pub_type
杂志文章abstract::We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter freundii that was found to produce a factor in the culture supernatant that cross-reacted with antibodies to CTB and LTB when...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.10.4222-4228.1997
更新日期:1997-10-01 00:00:00
abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:1991-06-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.2.458-467.1989
更新日期:1989-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.3.582-588.1988
更新日期:1988-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.69.11.7140-7145.2001
更新日期:2001-11-01 00:00:00
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更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.284-285.1974
更新日期:1974-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.480-488.1978
更新日期:1978-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.2.644-652.1996
更新日期:1996-02-01 00:00:00
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doi:10.1128/IAI.62.12.5698-5701.1994
更新日期:1994-12-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.27.1.231-234.1980
更新日期:1980-01-01 00:00:00
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更新日期:2011-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.6.742-749.1970
更新日期:1970-12-01 00:00:00
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更新日期:1997-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4629-4636.1993
更新日期:1993-11-01 00:00:00
abstract::We have investigated the possibility that nitric oxide (NO) synthesis may affect the course of a trypanosome infection via T-cell responses using mice deficient in inducible NO synthase (iNOS). Parasitemia levels increased at the same rate in both iNOS-deficient homozygous and control heterozygous mice, and peak paras...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3334-3338.1999
更新日期:1999-07-01 00:00:00
abstract::Salivary proline-rich proteins (PRPs), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to Actinomyces viscosus type 1 fimbriae. Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum album...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1986-1991.1990
更新日期:1990-06-01 00:00:00