Abstract：
:We investigated if fermentation with probiotic cultures could improve the production of health-promoting biological compounds in Astragalus membranaceus. We tested the probiotics Enterococcus faecium, Lactobacillus plantarum and Enterococcus faecium + Lactobacillus plantarum and applied PacBio single molecule, real-time sequencing technology (SMRT) to evaluate the quality of Astragalus fermentation. We found that the production rates of acetic acid, methylacetic acid, aethyl acetic acid and lactic acid using E. faecium + L. plantarum were 1866.24 mg/kg on day 15, 203.80 mg/kg on day 30, 996.04 mg/kg on day 15, and 3081.99 mg/kg on day 20, respectively. Other production rates were: polysaccharides, 9.43%, 8.51%, and 7.59% on day 10; saponins, 19.6912 mg/g, 21.6630 mg/g and 20.2084 mg/g on day 15; and flavonoids, 1.9032 mg/g, 2.0835 mg/g, and 1.7086 mg/g on day 20 using E. faecium, L. plantarum and E. faecium + L. plantarum, respectively. SMRT was used to analyze microbial composition, and we found that E. faecium and L. plantarum were the most prevalent species after fermentation for 3 days. E. faecium + L. plantarum gave more positive effects than single strains in the Astragalus solid state fermentation process. Our data demonstrated that the SMRT sequencing platform is applicable to quality assessment of Astragalus fermentation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Qiao H,Zhang X,Shi H,Song Y,Bian C,Guo Adoi
10.1038/s41598-018-30288-xsubject
Has Abstractpub_date
2018-08-08 00:00:00pages
11862issue
1issn
2045-2322pii
10.1038/s41598-018-30288-xjournal_volume
8pub_type
杂志文章abstract：:Biofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies were efficient in controlling...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81977-z
更新日期：2021-01-27 00:00:00
abstract：:Prestin in the lateral membrane of outer hair cells, is responsible for electromotility (EM) and a corresponding nonlinear capacitance (NLC). Prestin's voltage sensitivity is influenced by intracellular chloride. A regulator of intracellular chloride is a stretch-sensitive, non-selective conductance within the lateral...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46619
更新日期：2017-04-19 00:00:00
abstract：:Fuelled by a desire for greater connectivity, networked systems now pervade our society at an unprecedented level that will affect it in ways we do not yet understand. In contrast, nature has already developed efficient networks that can instigate rapid response and consensus when key elements are stimulated. We prese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53942-4
更新日期：2019-11-26 00:00:00
abstract：:Impedance, or oscillometry, measurements of the respiratory system can generate information about the function of the respiratory system not possible with traditional spirometry. There are currently several instruments on the market using different perturbations. We have compared a new respiratory oscillometry instrum...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48039-x
更新日期：2019-08-12 00:00:00
abstract：:The Casimir force, originating from vacuum zero-point energy, is one of the most intriguing purely quantum effects. It has attracted renewed interests in current field of nanomechanics, due to the rapid size decrease of on-chip devices. Here we study the optomechanically-induced transparency (OMIT) with a tunable Casi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27102
更新日期：2016-06-03 00:00:00
abstract：:Liquid biopsy can provide a strong basis for precision medicine. We aimed to identify novel single-nucleotide variants (SNVs) in circulating tumor DNA (ctDNA) in patients with hepatocellular carcinoma (HCC). Deep sequencing of plasma-derived ctDNA from 59 patients with HCC was performed using a panel of 2924 SNVs in 6...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74494-y
更新日期：2020-10-20 00:00:00
abstract：:Cellular therapies for liver diseases and in vitro models for drug testing both require functional human hepatocytes (Hum-H), which have unfortunately been limited due to the paucity of donor liver tissues. Human pluripotent stem cells (hPSCs) represent a promising and potentially unlimited cell source to derive Hum-H...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16884
更新日期：2015-11-23 00:00:00
abstract：:There is an urgent need to quantify anthropogenic influence on forest carbon stocks. Using satellite-based radar imagery for such purposes has been challenged by the apparent loss of signal sensitivity to changes in forest aboveground volume (AGV) above a certain 'saturation' point. The causes of saturation are debate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03469-3
更新日期：2017-06-14 00:00:00
abstract：:Hypoxia-induced retinal neovascularization is a major pathological condition in many vision-threatening diseases. In the present study, we determined whether interleukin (IL)-12, a cytokine that regulates angiogenesis, plays a role in the neovascularization in a mouse model of oxygen-induced retinopathy (OIR). We foun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28140
更新日期：2016-06-17 00:00:00
abstract：:Ncb2, the β subunit of NC2 complex, a heterodimeric regulator of transcription was earlier shown to be involved in the activated transcription of CDR1 gene in azole resistant isolate (AR) of Candida albicans. This study examines its genome-wide role by profiling Ncb2 occupancy between genetically matched pair of azole...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46084
更新日期：2017-04-06 00:00:00
abstract：:Repeated social defeat stress (R-SDS) induces multiple behavioral changes in mice. However, the relationships between these behavioral changes were not fully understood. In the first experiment, to examine how the social avoidance is related to R-SDS-impaired behavioral flexibility, 10-week-old male C57BL/6N mice rece...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28803-1
更新日期：2018-07-11 00:00:00
abstract：:Cancer dissemination is initiated by the movement of cells into the vasculature which has been reported to be triggered by EMT (epithelial to mesenchymal transition). Cellular dissemination also requires proteases that remodel the extracellular matrix. The protease, plasmin is a prominent player in matrix remodeling a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32433-y
更新日期：2018-09-20 00:00:00
abstract：:Magnetic skyrmions are promising candidates for the next generation of spintronic devices due to their small size and topologically protected structure. One challenge for using these magnetic states in applications lies on controlling the nucleation process and stabilization that usually requires an external force. He...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40705-4
更新日期：2019-03-11 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-49306-7
更新日期：2019-09-03 00:00:00
abstract：:Channelrhodopsin-2 (ChR2)-based optogenetic technique has been increasingly applied to cardiovascular research. However, the potential effects of ChR2 protein overexpression on cardiomyocytes are not completely understood. The present work aimed to examine how the doxycycline-inducible lentiviral-mediated ChR2 express...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01723-2
更新日期：2017-05-08 00:00:00
abstract：:Zika virus infection and dengue and chikungunya fevers are emerging viral diseases that have become public health threats. Their aetiologic agents are transmitted by the bite of genus Aedes mosquitoes. Without effective therapies or vaccines, vector control is the main strategy for preventing the spread of these disea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39666-5
更新日期：2019-03-11 00:00:00
abstract：:Recent advances in next-generation sequencing technologies have paved the path for a considerable amount of sequencing data at a relatively low cost. This has revolutionized the genomics and transcriptomics studies. However, different challenges are now created in handling such data with available bioinformatics platf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75270-8
更新日期：2020-10-26 00:00:00
abstract：:A versatile method based on Raman microscopy was developed to follow the degradation of iron carboxylate Metal Organic Framework (MOF) nano- or micro-particles in simulated body fluid (phosphate buffer). The analysis of both the morphology and chemical composition of individual particles, including observation at diff...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13323-1
更新日期：2017-10-13 00:00:00
abstract：:The Spike protein of the novel coronavirus SARS-CoV2 contains an insertion 680SPRRAR↓SV687 forming a cleavage motif RxxR for furin-like enzymes at the boundary of S1/S2 subunits. Cleavage at S1/S2 is important for efficient viral entry into target cells. The insertion is absent in other CoV-s of the same clade, includ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74101-0
更新日期：2020-10-09 00:00:00
abstract：:Earth's orbital variations on timescales of 104-105 years, known as Milankovitch cycles, have played a critical role in pacing climate change and ecosystem dynamics, through glacial and/or monsoon dynamics. However, the climatic and biotic consequences of these cycles on much longer (~ 107 years) timescales remain unc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68542-w
更新日期：2020-07-23 00:00:00
abstract：:Urbanization is associated with dramatic increases in noise and light pollution, which affect animal behaviour, physiology and fitness. However, few studies have examined these stressors simultaneously. Moreover, effects of urbanization during early-life may be detrimental but are largely unknown. In developing great ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02940-5
更新日期：2017-06-05 00:00:00
abstract：:Enterococcus faecium is a commensal of the mammalian gastrointestinal tract, but is also found in non-enteric environments where it can grow between 10 °C and 45 °C. E. faecium has recently emerged as a multi-drug resistant nosocomial pathogen. We hypothesized that genes involved in the colonization and infection of m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18255
更新日期：2015-12-17 00:00:00
abstract：:Dendritic cell (DC)-derived exosomes (Dexo) contain the machinery necessary to activate potent antigen-specific immune responses. As promising cell-free immunogens, Dexo have been tested in previous clinical trials for cancer vaccine immunotherapy, yet resulted in limited therapeutic benefit. Here, we explore a novel ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17622
更新日期：2015-12-03 00:00:00
abstract：:HMG domain transcription factor, Sox2, is a critical gene for the development of cochlear hair cells, the receptor cells for hearing, but this has been ascribed to expansion of the progenitors that become hair cells. Here, we show that Sox2 activated Atoh1, a transcription factor important for hair cell differentiatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23293
更新日期：2016-03-18 00:00:00
abstract：:In June 2016, a disease characterised by intestinal haemorrhage with a mortality rate of approximately 5% was observed in a duck farm in Shandong province, China. Here, we report the isolation and characterisation of a reovirus from duck tissue samples by inoculating duck embryos and duck embryo fibroblasts (DEF). The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44178-3
更新日期：2019-05-23 00:00:00
abstract：:Vitamin D and parathyroid hormone (PTH) regulate mineral metabolism and are required to maintain calcium levels. Vitamin D deficiency is common, particularly during pregnancy, and has been associated with hypertensive disorders of pregnancy. We sought to determine whether maternal 25(OH)D, PTH and calcium concentratio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37600-9
更新日期：2019-02-04 00:00:00
abstract：:Biomimetic scaffolds are of great interest to tissue engineering (TE) and tissue repair as they support important cell functions. Scaffold coating with soluble collagen-I has been used to achieve better tissue integration in orthopaedy, however, as collagen persistence was only temporary such efforts were limited. Ade...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27760-z
更新日期：2018-06-20 00:00:00
abstract：:Our study aimed to compare the ability of anthropometric obesity indices to predict MetS and to determine the sex-specific optimal cut-off values for MetS among Malaysian vegetarians. Body weight, height, waist circumference (WC), blood pressure (BP), fasting venous blood sample were collected from 273 vegetarians in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78035-5
更新日期：2020-11-30 00:00:00
abstract：:Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic resolution, electron beam induced specimen damage precludes high resolution imaging of sensitive materials, such as single proteins or polymers. Here, we use simulat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01841-x
更新日期：2017-05-10 00:00:00
abstract：:Archeological researches have proposed arguments for human mobility and long-distance trading over the Eurasia before the Silk Roads. Here we utilize biologically available strontium isotope analysis to assess the extent of pre-Silk Road population movements and cultural communications across the Asian interior. From ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35162
更新日期：2016-10-20 00:00:00
