Abstract：
:One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was cloned into pET-28a(+) expression vector and transformed into Escherichia coli. A 52.6 kDa silk protein was successfully expressed and detected by SDS-PAGE and confirmed by Western blotting. A maximum yield of the silk protein was expressed with 7.06 mM IPTG after 5 h incubation. This is the first report on the construction and overexpression of a A. ventricosus dragline silk multimeric gene construct and the results from our study will provide a reference point for further exploration and development of large-scale production of spider silk protein.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Liu T,Liang A,Liang Z,Li G,Wang Fdoi
10.1007/s13205-018-1285-0subject
Has Abstractpub_date
2018-05-01 00:00:00pages
252issue
5eissn
2190-572Xissn
2190-5738pii
1285journal_volume
8pub_type
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