Abstract:
:To decipher an evolutionary lineage between two different but important bacterial groups, i.e., Pseudomonas strain (γ-Proteobacteria) and Frankia strain (actinobacteria) growing in the same ecological niche in and around of an actinorhizal plant Hippophae salicifolia D. Don, genetic diversity and comparative molecular phylogeny have been investigated using 16S rRNA gene sequences and computer-simulated and virtually directed restriction fragment length polymorphism (RFLP) through 10 restriction enzymes. Bayesian and coalescent analyses on the basis of 16S rRNA gene sequences suggested three major groups with close proximity between Pseudomonas and Frankia isolates. This result has been further validated based on the data observed through similarity coefficient value and computational RFLP. Principal component analysis and Mandel h and k statistical analysis also confirmed and strengthen the findings. Approximately 458 aligned sequence of all the taxa were used to decipher nucleotide diversity, polymorphism and gene flow between these taxa. Thus, our results suggest for a possible co-evolution or a heterologous gene transfer of distantly related microbial forms. Further, our study also advocate for the use of computer aided, virtual RFLP analysis as a cost effective and rapid identification tool.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Srivastava M,Kaushik MS,Srivastava A,Singh A,Verma E,Mishra AKdoi
10.1007/s13205-016-0488-5subject
Has Abstractpub_date
2016-12-01 00:00:00pages
178issue
2eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-016-0488-5journal_volume
6pub_type
杂志文章相关文献
3 Biotech文献大全abstract::Hyaluronic acid (HA) is a biopolymer used in several industries. There is increasing global demand. HA is normally produced on a large scale using attenuated strains of group C streptococci that are pathogenic and fastidious. Accordingly, it is of interest to use a "generally recognized as safe" (GRAS) organism such a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1749-x
更新日期:2019-06-01 00:00:00
abstract::Mistletoes are a group of obligate plant semi-parasites in the order Santalales. These clumps of plants growing on a wide range of host plants have been traditionally regarded as medicinal repositories. However, current scientific discoveries have validated their health potentials like never before. Their extracts con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0124-6
更新日期:2014-02-01 00:00:00
abstract::Tomato (Solanum lycopersicum) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and dur...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1665-0
更新日期:2019-04-01 00:00:00
abstract::The present study was aimed to evaluate the immuno-modulatory effect of Brucella-specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice (n = 6 ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1905-3
更新日期:2019-10-01 00:00:00
abstract::In the present study, we have experimentally and theoretically studied the free-radical quenching property of dihydrocanaric acid (DCA) isolated from seedpods of Holarrhena antidysenterica. A modified method was used to estimate the nitric oxide scavenging effect of the DCA (significant activity of 75.22%) along with ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02221-5
更新日期:2020-06-01 00:00:00
abstract::Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0594-4
更新日期:2017-05-01 00:00:00
abstract::Alkaline hydrogen peroxide (AHP) as a pretreatment effectively enhances the increasing enzymatic digestibility of oil palm trunk (OPT) for conversion to biofuels and bioproducts in the biorefinery processes. The effect of hydrogen peroxide concentration (1-5%), temperature (50-90 °C), and time (30-90 min) were studied...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02169-6
更新日期:2020-04-01 00:00:00
abstract::The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was inves...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0795-5
更新日期:2017-07-01 00:00:00
abstract::The purpose of this review is to assist readers in understanding the importance of Lupinus albescens to nature, farmers, and scientists. L. albescens is mostly found in Argentina, Uruguay, Paraguay, and in "Campanha, Litoral and Missões" regions of State of Rio Grande do Sul (Brazil). Therefore, this review presents i...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-018-1474-x
更新日期:2018-10-01 00:00:00
abstract::Bio-inoculants play an important role for sustainable agriculture. Application of nanocompounds in the agriculture sector provides strength and is reported to enhance crop production but the combined effect of nanocompounds and plant growth-promoting rhizobacteria on plants has not been studied much. Therefore, the pr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02561-2
更新日期:2021-01-01 00:00:00
abstract::Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0552-1
更新日期:2016-12-01 00:00:00
abstract::In this study, we examined the responses by the indigenous bacterial communities in salt-marsh sediment microcosms in vitro following treatment with Mississippi Canyon Block 252 oil (MC252). Microcosms were constructed of sediment and seawater collected from Bayou La Batre located in coastal Alabama on the Gulf of Mex...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-014-0233-x
更新日期:2015-08-01 00:00:00
abstract::The tolerance of the pentose-fermenting yeast Meyerozyma guilliermondii to the inhibitors released after the biomass hydrolysis, such as acetic acid and furfural, was surveyed. We first verified the effects of acetic acid and cell concentrations and initial pH on the growth of a M. guilliermondii strain in a semi-synt...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1143-0
更新日期:2018-02-01 00:00:00
abstract::We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars 'Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both culti...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1408-7
更新日期:2018-09-01 00:00:00
abstract::In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1971-6
更新日期:2019-11-01 00:00:00
abstract::Plant growth-promoting fungi play an important role in development of sustainable agriculture. In the current study, 13 fungal strains were isolated from the rhizosphere of healthy Triticum aestivum (wheat) plant and screened for their indolic auxin production potential. Aspergillus flavus strain PGFW, Aspergillus nig...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1428-3
更新日期:2018-10-01 00:00:00
abstract::RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predict...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1893-3
更新日期:2019-10-01 00:00:00
abstract::High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1330-z
更新日期:2018-07-01 00:00:00
abstract::The study investigates the ability of methanolic extract of Andrographis paniculata (MAP) to supplement polyvalent anti-snake venom (ASV) in inhibiting neurotoxic enzyme acetylcholinesterase (AChE) and 'spreading factor' hyaluronidase from Naja naja (N.N) venom. AChE and hyaluronidase activity were measured in 100 or ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02462-4
更新日期:2020-11-01 00:00:00
abstract::Of the two commercially cultivated coffee (Coffea) species, C. arabica (arabica) is highly susceptible and C. canephora (robusta) is highly resistant to the insect pest Xylotrechus quadripes (Coleoptera: Cerambycidae), commonly known as coffee white stem borer (CWSB). We constructed a forward-subtracted cDNA library b...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0715-8
更新日期:2017-05-01 00:00:00
abstract::In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02359-2
更新日期:2020-08-01 00:00:00
abstract::The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0682-0
更新日期:2017-05-01 00:00:00
abstract::The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-st...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-014-0213-1
更新日期:2015-04-01 00:00:00
abstract::Human leukocyte antigen (HLA)-F has been involved in immune regulation of infectious diseases. However, the role of HLA-F polymorphisms in hepatitis B infection outcomes remains unclear. Here, we aimed to determine HLA-F polymorphism implication in chronic HBV. Genotype analysis was performed for three single nucleoti...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1079-9
更新日期:2018-01-01 00:00:00
abstract::Thermal stress is one of the challenges to crop plants that negatively impacts crop yield. To overcome this ever-growing problem, utilization of regulatory mechanisms, especially microRNAs (miRNAs), that provide efficient and precise regulation in a targeted manner have been found to play determining roles. Besides th...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-018-1521-7
更新日期:2018-12-01 00:00:00
abstract::Graminaceous plants secrete hydroxylated phytosiderophores encoded by the genes iron-deficiency-specific clone 2 (Ids2) and iron-deficiency-specific clone 3 (Ids3). An effort was made to identify a putative ortholog of Hodeum vulgare Ids3 gene in hexaploid wheat. The protein structure of TaIDS3 was modeled using homol...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1230-2
更新日期:2018-04-01 00:00:00
abstract::The natural estrogen 17β-estradiol (17β-E2) is a major endocrine disruptor. Accordingly, due to their frequent presence in global surface waters, prolonged exposure to estrogen-contaminated water may disrupt sexual development in animals. It has adverse effects on wildlife and humans. To date, the most effective strat...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-020-2155-0
更新日期:2020-04-01 00:00:00
abstract::This bibliometric-based review analyses the trends in 1010 published articles (1990 to mid-2018) on the high-value application of Chlorella, and illustrates the evolution and latest tendencies at a global level by the number of publications and their distribution, issuing institutions and countries or regions, the sou...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2102-0
更新日期:2020-03-01 00:00:00
abstract::Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2053-5
更新日期:2020-02-01 00:00:00
abstract::This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechan...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02237-x
更新日期:2020-06-01 00:00:00