Abstract:
:The natural estrogen 17β-estradiol (17β-E2) is a major endocrine disruptor. Accordingly, due to their frequent presence in global surface waters, prolonged exposure to estrogen-contaminated water may disrupt sexual development in animals. It has adverse effects on wildlife and humans. To date, the most effective strategy for estrogen removal from the environment is biodegradation using microorganisms. To this end, we isolated a strain of Lysinibacillus sphaericus, namely DH-B01, from a contraceptive factory in Beijing. The experimental results revealed that the bacterium has a high capacity to degrade estrogen, with a 17β-E2 degradation rate of about 97%, and produces the secondary metabolite estrone. In addition, a series of genes involved in steroid metabolism and stress response in L. sphaericus sp. DH-B01 were predicted, and several key genes with high similarity to those of other strains were subjected to sequence alignment to find their conserved regions. This is the first study of the ability of L. sphaericus strains to degrade estrogens and the degradation mechanism involved. This work advances the genomic study of estrogen-degrading strains and the study of bacterial estrogen degradation mechanisms. In this paper, a novel bacterial strain capable of degrading 17β-E2 was studied. L. sphaericus sp. DH-B01 can effectively degrade 17β-E2. During the degradation process, 17β-E2 can be gradually metabolized to a substance without estrogen activity. By analyzing the enzymatic reactions in the metabolic process, we found genes with high similarity to reported 17β-HSD. L. sphaericus sp. DH-B01 was found to degrade 17β-E2. There are many types of bacteria that are currently being studied for the degradation of estrogen, but L. sphaericus sp. DH-B01 is the only strain of L. sphaericus that has been shown to degrade estrogen. This work advances the genomic study of estrogen-degrading bacterial strains and the study of bacterial estrogen degradation mechanisms. Additionally, it explores the correlation between different L. sphaericus strains. The differences play an important role and further enrich the functionality and diversity of L. sphaericus strains. In subsequent studies, the specificity of L. sphaericus sp. DH-B01 can be applied to different environments for future environmental restoration.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Wang Y,Zhao X,Tian K,Meng F,Zhou D,Xu X,Zhang H,Huo Hdoi
10.1007/s13205-020-2155-0subject
Has Abstractpub_date
2020-04-01 00:00:00pages
166issue
4eissn
2190-572Xissn
2190-5738pii
2155journal_volume
10pub_type
相关文献
3 Biotech文献大全abstract::An efficient regeneration system via a combined pathway of organogenesis and somatic embryogenesis was developed for date palm (Phoenix dactylifera L.) cv. Mejhoul. Adventitious buds were obtained from shoot-tip explants with a frequency of 53.3% after 9 months of culture: 6 months on half-strength Murashige and Skoog...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1235-x
更新日期:2018-04-01 00:00:00
abstract::Statistical methodologies, including Plackett-Burman design and Box-Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for max...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1015-z
更新日期:2017-12-01 00:00:00
abstract::Bio-inoculants play an important role for sustainable agriculture. Application of nanocompounds in the agriculture sector provides strength and is reported to enhance crop production but the combined effect of nanocompounds and plant growth-promoting rhizobacteria on plants has not been studied much. Therefore, the pr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02561-2
更新日期:2021-01-01 00:00:00
abstract::Advancement in nanotechnology has improved ways for large-scale production and characterization of nanoparticles of physiologically important metals. The current study explores the impact of Zinc Oxide Nanoparticles (ZnO-NP) and Chitosan-Zinc oxide nano-bioformulation (CH-ZnO) in tissue culture raised callus of Nicoti...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02469-x
更新日期:2020-11-01 00:00:00
abstract::This paper has studied the success of implantation for 16 commercial active dry yeasts (ADYs) during industrial fermentation (30) and the impact of these yeasts during spontaneous fermentations (19) in 10 wineries from the Denomination of Origin "Vinos de Madrid" over two consecutive years. Yeasts strains were identif...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1913-3
更新日期:2019-11-01 00:00:00
abstract::The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After scr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0579-3
更新日期:2017-05-01 00:00:00
abstract::New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-011-0010-z
更新日期:2011-09-01 00:00:00
abstract::Energy efficiency ratio is significant in completely estimating lignocellulosic biomass pretreatment. In this work, rice straw (RS) was pretreated by ultra-high pressure (UHP), ionic liquid microemulsion (ILM), and a combination of UHP and ILM (ILM + UHP) at mild temperature. The chemical composition, crystalline stru...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1216-0
更新日期:2018-04-01 00:00:00
abstract::One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was clo...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1285-0
更新日期:2018-05-01 00:00:00
abstract::The wound healing process is characterized by varied biological and molecular cascades including inflammation, tissue proliferation, and remodeling phase. To augment and maintain these cascades, an all-natural matrix system is proposed. Biocompatible biopolymers, sodium alginate and gelatin, were employed to prepare m...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02453-5
更新日期:2020-11-01 00:00:00
abstract::Forty-three bacterial isolates in five genera were recovered from naturally infected green pepper fruits (38 samples) showing dark brown, irregular-shaped splotches. The pathogenicity test was performed on healthy green pepper fruits and red colonies were from inoculated fruits showing the same symptoms and the infect...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0136-2
更新日期:2014-08-01 00:00:00
abstract::Tomato (Solanum lycopersicum) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and dur...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1665-0
更新日期:2019-04-01 00:00:00
abstract::Hydrogen production by biological route is a potentially sustainable alternative. Nowadays, energy production from sustainable sources has become urgent for several countries as well as for international policies. In this perspective, hydrogen has gained substantial global attention as clean, sustainable, and versatil...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1676-x
更新日期:2019-04-01 00:00:00
abstract::Genetic polymorphism amid plant species is a crucial factor for plant improvement and maintaining their biodiversity. Evaluation of genetic diversity amongst plant species is significant to deal with the environmental stress conditions and their effective involvement in the breeding programs. Hence, in present study, ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1736-2
更新日期:2019-06-01 00:00:00
abstract::We present here a modified, sonication-assisted transient transformation assay for rapid analysis of cis-regulatory elements. We tested promoter elements from MIR159B locus of Brassica juncea by generating stable transgenic lines and compared the transcriptional activity of GUS reporter with that of the transient assa...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1219-x
更新日期:2018-04-01 00:00:00
abstract::The aim of this study was to explore the neuroprotective effect and the underlying mechanism of erythropoietin (EPO) on the cortical neuronal cells insulted with oxygen and glucose deprivation (OGD). Different concentrations of EPO were used to determine the anti-apoptosis effect of EPO. In addition, PI3K inhibitor LY...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1667-y
更新日期:2019-04-01 00:00:00
abstract::Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0788-4
更新日期:2017-07-01 00:00:00
abstract::Uridine diphosphate (UDP)-glycosyltransferases (UGTs) involved in many metabolic processes are ubiquitous in plants, animals, microorganisms and other organisms and are essential for their growth and development. Upland cotton contains a large number of UGT genes. In this study, we aimed to identify UGT family members...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1984-1
更新日期:2019-12-01 00:00:00
abstract::A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5-1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0134-4
更新日期:2014-04-01 00:00:00
abstract::Novel coronavirus disease 2019 (COVID-19) is a positive-sense single-stranded RNA virus which belongs to the Coronaviridae family. COVID-19 outbreak became evident after the severe acute respiratory syndrome coronavirus and the Middle East respiratory syndrome coronavirus in the twenty-first century as the start of th...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-021-02657-3
更新日期:2021-02-01 00:00:00
abstract::In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. R...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1477-7
更新日期:2018-11-01 00:00:00
abstract::The present study was conducted to investigate the antileishmanial activity of biogenic silver nanoparticles (AgNPs) compared to chemically synthesized AgNPs. A nano dimension size (10-15 nm) biogenic AgNPs was produced and characterized by UV-Vis spectroscopy and X-rays diffraction. The chemically synthesized AgNPs w...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1121-6
更新日期:2018-02-01 00:00:00
abstract::Temperate and dry deciduous forest covers major portion of terrestrial ecosystem in India. The two forest types with different dominant tree species differ in litter quality and root exudates, thereby exerting species-specific impact on soil properties and microbial activity. This study aims to examine the influence o...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0510-y
更新日期:2016-12-01 00:00:00
abstract::The present study was evaluated to test the potential of plant growth-promoting actinobacteria in increasing seed mineral density of chickpea under field conditions. Among the 19 isolates of actinobacteria tested, significant (p < 0.05) increase of minerals over the uninoculated control treatments was noticed on all t...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0458-y
更新日期:2016-12-01 00:00:00
abstract::The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1489-3
更新日期:2018-11-01 00:00:00
abstract::Several actinomycetes strains were isolated from different marine sponges collected from the Red Sea shore in Egypt. The efficiency of their crude extracts to inhibit histone deacetylase (HDAC) enzyme was investigated in the nuclear extract of Hela cell line. The crude extract corresponding to Streptomyces sp. SP9 iso...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1304-1
更新日期:2018-06-01 00:00:00
abstract::Salmonella typhimurium (S. typhimurium) represents an important global public health problem and has the ability to survive under desiccation conditions in foods and food processing facilities for years. The aim of this study was to investigate the effects of Allium sativum (A. sativum) and Cuminum cyminum (C. cyminum...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02286-2
更新日期:2020-07-01 00:00:00
abstract::Although endophytic bacteria are known to colonize Panax ginseng, little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-018-1417-6
更新日期:2018-09-01 00:00:00
abstract::Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of Tribulus terrestris was screened upon chromoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1695-7
更新日期:2019-04-01 00:00:00
abstract::Electroporation parameters can be optimized by coupling RC constant values with the amount of electrical power dissipation in the electroporation medium. Electroporation efficiency increases more steeply with power at low power values. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0105-1
更新日期:2013-08-01 00:00:00