Abstract:
:In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the eGFP expression cassette or promoter in a forward/reverse orientation. After stable transfection into CHO-K1 cells with these vectors, eGFP expression levels were increased significantly relative to that of the control vector (MAR-devoid) when two MARs flanking the expression cassette were incorporated, followed by those at the 5' site (upstream of the promoter). Simultaneously, the percentage of the eGFP-expressing cells was elevated to some extent. The vector with both MARs in forward orientation flanking the expression cassette yielded the highest transgene expression levels (2.5-fold). The orientation (forward or reverse) of the MARs did not present a significant difference when added in the same site. In addition, transgene expression levels were not exclusively dependent on transgene copy numbers. Bioinformatic analysis indicated that some specific transcription factors may contribute to the transcriptional process. In conclusion, two MARs in a forward orientation and flanking the expression cassette comprised the optimal construct for improving the stable transgene expression in the CHO-K1 cells. The effects may be related to specific transcription factors, such as PRDM1 and REL.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Zhang J,Zhang J,Cheng S,Yang W,Li Sdoi
10.1007/s13205-019-1971-6subject
Has Abstractpub_date
2019-11-01 00:00:00pages
435issue
11eissn
2190-572Xissn
2190-5738pii
1971journal_volume
9pub_type
杂志文章相关文献
3 Biotech文献大全abstract::Low conversion efficiency and long-processing time are some of the major problems associated with the use of biocatalysts in industrial processes. In this study, modified magnetic iron oxide nanoparticles bearing tyrosinase (tyrosinase-MNPs) were employed as a magnetic nano-biocatalyst to treat phenol-containing waste...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1445-2
更新日期:2018-10-01 00:00:00
abstract::The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0682-0
更新日期:2017-05-01 00:00:00
abstract::Tomato (Solanum lycopersicum) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and dur...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1665-0
更新日期:2019-04-01 00:00:00
abstract::The tolerance of the pentose-fermenting yeast Meyerozyma guilliermondii to the inhibitors released after the biomass hydrolysis, such as acetic acid and furfural, was surveyed. We first verified the effects of acetic acid and cell concentrations and initial pH on the growth of a M. guilliermondii strain in a semi-synt...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1143-0
更新日期:2018-02-01 00:00:00
abstract::In the presented research the extracellular chitinase of Stenotrophomonas rhizophila G22 was biochemically and molecularly characterized. The studied enzyme was purified from a 72-h bacterial culture about 14 times, with a recovery of 63%. The molecular weight of the purified protein was estimated at 50 kDa by SDS-PAG...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-2007-y
更新日期:2020-01-01 00:00:00
abstract::Metatranscriptomics, a subset of metagenomics, provides valuable information about the whole gene expression profiling of complex microbial communities of an ecosystem. Metagenomic studies mainly focus on the genomic content and identification of microbes present within a community, while metatranscriptomics provides ...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-020-2057-1
更新日期:2020-02-01 00:00:00
abstract::Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0109-x
更新日期:2013-12-01 00:00:00
abstract::The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1489-3
更新日期:2018-11-01 00:00:00
abstract::Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of mult...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0592-6
更新日期:2017-05-01 00:00:00
abstract::In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate fo...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1983-2
更新日期:2019-12-01 00:00:00
abstract::This study was aimed at investigating the phytochemical constituents, antifungal properties and antibiotic-modifying activity of the aqueous crude extract and fractions of Amburana cearensis seeds (CEFAC). The CEFAC were chemically characterized by LC-MS/MS-QTOF. In addition, the antifungal activity was assayed by the...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02551-4
更新日期:2020-12-01 00:00:00
abstract::The possibility of using Aspergillus terreus protease in detergent formulations was investigated. Sodium dodecyl sulfate (SDS) and native polyacrylamide gel electrophoresis indicated that the purified alkaline protease (148.9 U/mg) is a monomeric enzyme with a molecular mass of 16 ± 1 kDa. This was confirmed by liquid...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-014-0200-6
更新日期:2015-02-01 00:00:00
abstract::Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0834-2
更新日期:2017-07-01 00:00:00
abstract::Mistletoes are a group of obligate plant semi-parasites in the order Santalales. These clumps of plants growing on a wide range of host plants have been traditionally regarded as medicinal repositories. However, current scientific discoveries have validated their health potentials like never before. Their extracts con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0124-6
更新日期:2014-02-01 00:00:00
abstract::In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02359-2
更新日期:2020-08-01 00:00:00
abstract::Fusarium wilt is one of the main limiting factors for tomato production in Mexico. One thousand and fifty isolates were obtained from vascular tissues tomato plants showing wilt and yellowing symptoms in Sinaloa, Mexico. The pathogenic isolates were evaluated through phylogenetic analysis of the TEF-1α gene and ITS re...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1808-3
更新日期:2019-07-01 00:00:00
abstract::Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved wit...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1315-y
更新日期:2018-07-01 00:00:00
abstract::Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was select...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0764-z
更新日期:2017-06-01 00:00:00
abstract::A novel cadmium-resistant bacterium, Raoultella sp. strain X13, recently isolated from heavy metal-contaminated soil, and this strain can synthesize CdS quantum dots using cadmium nitrate [Cd(NO4)2] and l-cysteine. Biomineralization of CdS by strain X13 can efficiently remove cadmium from aqueous solution. To illumina...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1649-0
更新日期:2019-04-01 00:00:00
abstract::A one-step formic acid-catalyzed organosolv process using a low-boiling point acid-solvent system was studied for fractionation of sugarcane bagasse. Compared to H2SO4, the use of formic acid as a promoter resulted in higher efficiency and selectivity on removals of hemicellulose and lignin with increased enzymatic di...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1244-9
更新日期:2018-05-01 00:00:00
abstract::Viral diseases affecting sweetpotato are the most devastating and cause up to 98 % yield loss. In this paper, we report, meristem culture, graft transmission and virus indexing for management of viral pathogens in seven elite sweetpotato cultivars. Plantlets were developed in vitro from the apical meristematic dome wi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0080-6
更新日期:2013-04-01 00:00:00
abstract::The yeasts transmitted from seeds to sprouts might be used as probiotics for host plants. To investigate the inheritable yeasts of rice plants for probiotics, the fungal internal transcribed spacer (ITS) regions (ITS1 and ITS2) in rice sprouts were analyzed by Illumina-based sequencing. The fungal genera Candida, Mort...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1746-0
更新日期:2019-06-01 00:00:00
abstract::Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02413-z
更新日期:2020-10-01 00:00:00
abstract::The present study illustrates optimization and synergetic potential of alga Chlorella pyrenoidosa for lipid production and remediation of Dairy industry wastewater (DIWW) through response surface methodology (RSM). Maximum lipid productivity of 34.41% was obtained under 50% DIWW supplemented with 0 mg L-1 nitrate (NO3...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1342-8
更新日期:2018-08-01 00:00:00
abstract::Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purificati...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0495-6
更新日期:2016-12-01 00:00:00
abstract::High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1330-z
更新日期:2018-07-01 00:00:00
abstract::Bacillus paralicheniformis strain KMS 80 (MTCC No. 12704) is an isolate from the root tissues of rice (Oryza sativa L.) that displays biological nitrogen fixation and plant growth promoting abilities. Here, we report the complete genome sequence of this strain, which contains 4,566,040 bp, 4424 protein-coding genes, 8...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1242-y
更新日期:2018-05-01 00:00:00
abstract::A detailed understanding of the fate of xenobiotics introduced into the environment and the mechanisms involved in their biotransformation, biodegradation, and biosorption is essential to improve the efficiency of remediation techniques. Mycoremediation is a form of bioremediation technique that has become increasingl...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02177-6
更新日期:2020-04-01 00:00:00
abstract::Present study mainly aimed to ascertain the distribution characteristics of gene-based microsatellite loci and to develop polymorphic SSR markers from the already available transcriptome data of Elaeis guineensis Jacq, an important oil crop. From this study, we identified the sum of 5791 SSRs across 51,425 unigenes fr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02269-3
更新日期:2020-06-01 00:00:00
abstract::Eight exopolysaccharide (EPS) producing metal-removing marine bacteria were screened for mercury (Hg) sorption. Bacillus licheniformis with the highest MIC values and Hg sorption ability was selected for further study. Biosorption of Hg from aqueous solution by Bacillus licheniformis was studied with respect to the me...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0958-4
更新日期:2017-10-01 00:00:00