Abstract：
:Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes related to Perl's pathway were determined by real-time PCR. The activities of enzymes in Perl's pathway were also determined. The results were verified by comparison with RNA-Seq and metabolomics data. The results showed that there were high transcript levels of some genes encoding malate dehydrogenase (MDH), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), and pyruvate kinase (PK) at the early stage of germination (0.75 h). The enzymes MDH, PEPCK, PK, PDC, and ADH showed peaks in activity at around 0.75 h and 6 h during germination. The oxaloacetate concentration was high in poplar seeds at the early stage of germination. This study provides experimental data showing that Perl's pathway participates in supplying energy during the early stages of poplar seed germination, and lays the foundation for further studies on the complex metabolic processes that function during seed germination.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Qu C,Zhang S,Zhao H,Chen J,Zuo Z,Sun X,Cheng Y,Xu Z,Liu Gdoi
10.1007/s13205-020-02413-zsubject
Has Abstractpub_date
2020-10-01 00:00:00pages
418issue
10eissn
2190-572Xissn
2190-5738pii
2413journal_volume
10pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:The aim of this study was to explore the neuroprotective effect and the underlying mechanism of erythropoietin (EPO) on the cortical neuronal cells insulted with oxygen and glucose deprivation (OGD). Different concentrations of EPO were used to determine the anti-apoptosis effect of EPO. In addition, PI3K inhibitor LY...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1667-y
更新日期：2019-04-01 00:00:00
abstract：:DNA has been recognized as a promising natural medium for information storage. The expensive DNA synthesis process makes it an important challenge to utilize DNA nucleotides optimally and increase the storage density. Thus, a novel scheme is proposed for the storage of digital information in synthetic DNA with high st...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1868-4
更新日期：2019-09-01 00:00:00
abstract：:The natural estrogen 17β-estradiol (17β-E2) is a major endocrine disruptor. Accordingly, due to their frequent presence in global surface waters, prolonged exposure to estrogen-contaminated water may disrupt sexual development in animals. It has adverse effects on wildlife and humans. To date, the most effective strat...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-020-2155-0
更新日期：2020-04-01 00:00:00
abstract：:The present study was designed to isolate an array of zinc solubilizing bacteria (ZSB) and to characterize them for plant growth promotion (PGP) attributes with respect to Capsicum annuum L. For this purpose, seventy bacteria were procured from cow dung and screened for zinc solubilization (ZnO and ZnCO3). Where, isol...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-2033-9
更新日期：2020-02-01 00:00:00
abstract：:We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0150-4
更新日期：2014-06-01 00:00:00
abstract：:In the present study, we investigated radiation mitigating activities of Psidium guajava L. (P. guajava) against whole-body X- ray induced damages in albino Wistar rat model. The animals were orally administered with 200 mg/kg bodyweight of hydroalcoholic leaf extract of P. guajava for five consecutive days and on the...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02484-y
更新日期：2020-12-01 00:00:00
abstract：:2,4-dinitrotoluene (2,4-DNT) is a priority environmental xenobiotic pollutant which has toxic, mutagenic, and carcinogenic properties. Thus, its biodegradation by applying recent approaches such as taking advantage of plant-bacteria interactions is crucial. In this work, the genes from Burkholderia sp. R34, necessary ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1933-z
更新日期：2019-11-01 00:00:00
abstract：:Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different ferme...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0694-9
更新日期：2017-05-01 00:00:00
abstract：:In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) con...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02359-2
更新日期：2020-08-01 00:00:00
abstract：:The maximum yield of xylanase from Aureobasidium melanogenum PBUAP46 was 5.19 ± 0.08 U ml-1 when cultured in a production medium containing 3.89% (w/v) rice straw and 0.75% (w/v) NaNO3 as carbon and nitrogen sources, respectively, for 72 h. This enzyme catalyzed well and was relatively stable at pH 7.0 and room temper...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1586-y
更新日期：2019-02-01 00:00:00
abstract：:Anthocyanins are major water-soluble and dynamic colouring plant pigment present in plant tissues with the high antioxidant properties. The role of ammonium and potassium nitrate in the culture medium on anthocyanin augmentation is probed thoroughly, but the mechanism of its biosynthesis continues to be unclear. Hence...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1447-0
更新日期：2018-10-01 00:00:00
abstract：:In this investigation, potential inhibitors of α-amylase, one of the key regulatory enzymes in diabetes were characterized from the methanolic extract of Leucas ciliata Benth. (Lamiaceae), a traditional medicinal plant of the Western Ghats, southern India and the ethyl acetate extract of Streptomyces longisporoflavus ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02547-0
更新日期：2021-02-01 00:00:00
abstract：:After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0610-3
更新日期：2017-05-01 00:00:00
abstract：:Pythium aphanidermatum is one of the common causal pathogen of damping-off disease of beans (Phaseolus vulgaris L.) grown in Manipur. A total of 110 indigenous Trichoderma isolates obtained from North east India were screened for their biocontrol activity which can inhibit the mycelial growth of P. aphanidermatum, the...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0027-3
更新日期：2011-12-01 00:00:00
abstract：:In this study, we report the isolation and characterization of the mRNA encoding OPAQUE2 (O2) like TF of finger millet (FM) (Eleusine coracana) (EcO2). Full-length EcO2 mRNA was isolated using conserved primers designed by aligning O2 mRNAs of different cereals followed by 3' and 5' RACE (Rapid Amplification of cDNA E...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1150-1
更新日期：2018-03-01 00:00:00
abstract：:Forty-three bacterial isolates in five genera were recovered from naturally infected green pepper fruits (38 samples) showing dark brown, irregular-shaped splotches. The pathogenicity test was performed on healthy green pepper fruits and red colonies were from inoculated fruits showing the same symptoms and the infect...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0136-2
更新日期：2014-08-01 00:00:00
abstract：:Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to sig...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0415-9
更新日期：2016-06-01 00:00:00
abstract：:This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechan...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02237-x
更新日期：2020-06-01 00:00:00
abstract：:The domestic yak (Bos grunniens) from the Qinghai-Tibet Plateau is an important animal model in high-altitude adaptation studies. Here, we performed the genome-wide selective sweep analysis to identify the candidate copy number variation (CNV) for the high-altitude adaptation of yaks. A total of 531 autosomal CNVs wer...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-020-02254-w
更新日期：2020-06-01 00:00:00
abstract：:Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2053-5
更新日期：2020-02-01 00:00:00
abstract：:The purpose of this study was to examine if fermentation of purple sweet potato (Ipomoea batatas L.) powder (PSP) by Lactobacillus brevis under green, red, blue, white light-emitting diode (LED) illumination or sunlight might yield functionalized products with good antibacterial, antioxidant activity, and/or cytotoxic...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1327-7
更新日期：2018-07-01 00:00:00
abstract：:The present study illustrates optimization and synergetic potential of alga Chlorella pyrenoidosa for lipid production and remediation of Dairy industry wastewater (DIWW) through response surface methodology (RSM). Maximum lipid productivity of 34.41% was obtained under 50% DIWW supplemented with 0 mg L-1 nitrate (NO3...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1342-8
更新日期：2018-08-01 00:00:00
abstract：:Bio-inoculants play an important role for sustainable agriculture. Application of nanocompounds in the agriculture sector provides strength and is reported to enhance crop production but the combined effect of nanocompounds and plant growth-promoting rhizobacteria on plants has not been studied much. Therefore, the pr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02561-2
更新日期：2021-01-01 00:00:00
abstract：:Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0755-0
更新日期：2017-07-01 00:00:00
abstract：:This paper investigated the effects of parameters, like inoculum size (15, 10 and 5% of the working volume of the reactor), gas velocities (0.0027, 0.00342 and 0.0068 m/s), bed heights (0.3, 0.6 and 0.9 m), static bed heights (4.85 and 2.43 cm), sizes of solid media particles (12, 4 mm), and the height to diameter rat...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1059-0
更新日期：2018-02-01 00:00:00
abstract：:The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1489-3
更新日期：2018-11-01 00:00:00
abstract：:Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast trans...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0315-4
更新日期：2016-06-01 00:00:00
abstract：:Human papilloma virus (HPV) is the primary etiological agent responsible for cervical cancer in women. Although in total 16 high-risk HPV strains have been identified so far. Currently available commercial vaccines are designed by targeting mainly HPV16 and HPV18 viral strains as these are the most common strains asso...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0352-z
更新日期：2016-06-01 00:00:00
abstract：:A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in E. coli was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by E. coli BL21 (pET22b-pelNK93I) was 10,181 U/mL which is the highest level so far. On this basis, to improve ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-2022-z
更新日期：2020-02-01 00:00:00
abstract：:Bacterial strains associated with entomopathogenic nematodes (EPNs) Rhabditis (Oscheius) spp. were isolated from infected cadavers of Galleria mellonella. The obtained 18 isolates were subdivided into nine phylogenetically different genera based on comparative sequence analysis of their 16S rRNA genes. The isolates we...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0326-1
更新日期：2016-06-01 00:00:00
