Abstract：
:The yeasts transmitted from seeds to sprouts might be used as probiotics for host plants. To investigate the inheritable yeasts of rice plants for probiotics, the fungal internal transcribed spacer (ITS) regions (ITS1 and ITS2) in rice sprouts were analyzed by Illumina-based sequencing. The fungal genera Candida, Mortierella, Alternaria, Penicillium, and Tomentella were revealed by both ITS1 and ITS2 sequence analysis. The endophytic yeasts were isolated from rice sprouts by yeast selective medium. Compared with the negative controls, inoculation of isolate Y3 released 2.2 folds higher concentration of free phosphate in soybean meal broth. Most of the phytase activities were located in the yeast cell interiors. The shoot lengths, shoot fresh weights, and root fresh weights of inoculated seedlings increased by 35%, 80%, and 60% compared with the control seedlings, respectively. The results suggested that the rice sprouts contained diverse phytase-producing yeasts transmitted from seeds. These yeasts might be adopted as prospective probiotics to improve rice growth by increasing phosphate utilization efficacy.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Zhu A,Tan H,Cao Ldoi
10.1007/s13205-019-1746-0subject
Has Abstractpub_date
2019-06-01 00:00:00pages
216issue
6eissn
2190-572Xissn
2190-5738pii
1746journal_volume
9pub_type
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