Abstract:
:The isolation of propiconazole (PCZ) degrading bacterium BBK_9 strain was done from paddy soil, and it was identified as Burkholderia sp. based on the morphological characteristics and biochemical properties combined with 16S rRNA gene sequencing analysis. It has been seen that the factors such as temperature and pH influence the biodegradation process. The role of plasmid was studied in the degradation process by plasmid curing method. The PCZ acts as the sole carbon source and as energy substrate which can be utilized by the strain for its growth in Mineral salt medium and degraded 8.89 µg ml-1 of PCZ at 30 °C and pH 7 within 4 days. During the bioconversion process of PCZ, three metabolite were formed such as 1-(2,4-dichlorophenyl)-2-(1H-1,2,4-triazol-1-yl) ethanone, 1-[2-(4-chlorophenyl) ethyl]-1H-1,2,4-triazole and 1-ethyl-1H-1,2,4-triazole. The LD50 value of BBK_9 strain was determined with acridine orange which resulted in 40 µg ml-1 at cell density of 0.243 at 660 nm. Furthermore, plasmid curing was done using LD50 concentration and from that three plasmids got cured in the sixth generation. It was found that, cured strain was able to degrade 7.37 µg ml-1 of PCZ, indicating the plasmid encoded gene were not responsible for the PCZ degradation. On the source of these outcomes, strain BBK_9 can be used as potential strain for bioremediation of contaminated sites.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Satapute P,Kaliwal Bdoi
10.1007/s13205-016-0429-3subject
Has Abstractpub_date
2016-06-01 00:00:00pages
110issue
1eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-016-0429-3journal_volume
6pub_type
杂志文章相关文献
3 Biotech文献大全abstract::Fungal pathogen, Tilletia indica, the cause of Karnal bunt disease in wheat, is severely affecting the yield and grain quality, worldwide. Thus, strict quarantine regulations by most wheat growing countries have to be followed, leading to trade barriers for wheat export. The conventional methods being used for pathoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02191-8
更新日期:2020-05-01 00:00:00
abstract::In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1971-6
更新日期:2019-11-01 00:00:00
abstract::Paddy soils represent the largest anthropogenic wetlands on earth. Soil drying and rewetting that occurs annually inflict significant stress on soil microbial activities in paddy soils. An incubation experiment of 60 years of paddy soil was conducted to simulate the conditions of paddy fields (25 °C and 75% air humidi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02486-w
更新日期:2020-11-01 00:00:00
abstract::Endophytes, by residing within the specific chemical environment of host plants, form unique group of microorganisms. Microbially unexplored medicinal plants can have diverse and potential microbial association. The rhizome of ginger is very remarkable because of its metabolite richness, but the physiological processe...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0143-3
更新日期:2014-04-01 00:00:00
abstract::RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predict...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1893-3
更新日期:2019-10-01 00:00:00
abstract::The maximum yield of xylanase from Aureobasidium melanogenum PBUAP46 was 5.19 ± 0.08 U ml-1 when cultured in a production medium containing 3.89% (w/v) rice straw and 0.75% (w/v) NaNO3 as carbon and nitrogen sources, respectively, for 72 h. This enzyme catalyzed well and was relatively stable at pH 7.0 and room temper...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1586-y
更新日期:2019-02-01 00:00:00
abstract::Achatina fulica (Lissachatina fulica) is one of the most invasive species found across the globe causing a significant damage to crops, vegetables, and horticultural plants. This terrestrial snail is native to east Africa and spread to different parts of the world by introductions. India, a hot spot for biodiversity o...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0877-4
更新日期:2017-08-01 00:00:00
abstract::Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ12-fatty acid desaturase (FAD)-encoding gene (Δ 12 CiFAD), 1269 bp in size, was cloned from Ch...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1858-6
更新日期:2019-09-01 00:00:00
abstract::Metatranscriptomics, a subset of metagenomics, provides valuable information about the whole gene expression profiling of complex microbial communities of an ecosystem. Metagenomic studies mainly focus on the genomic content and identification of microbes present within a community, while metatranscriptomics provides ...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-020-2057-1
更新日期:2020-02-01 00:00:00
abstract::Pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. Optimization of pectin extraction from muskmelon peel by response surface methodology (RSM) was investigated in this study. Box-Behnken Design (BBD) was used to identify the optimal level of the extraction variable...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0655-3
更新日期:2017-05-01 00:00:00
abstract::Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-020-2067-z
更新日期:2020-02-01 00:00:00
abstract::Selection of the raw material and its efficient utilization are the critical factors in economization of second generation (2G) ethanol production. Fermentation of the released sugars into ethanol by a suitable ethanol producing microorganism using cheap media ingredients is the cornerstone of the overall process. Thi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0098-9
更新日期:2013-10-01 00:00:00
abstract::New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-011-0010-z
更新日期:2011-09-01 00:00:00
abstract::In fishes, trypsins are adapted to different environmental conditions, and the biochemical and kinetic properties of a broad variety of native isoforms have been studied. Proteolytic enzymes remain in high demand in the detergent, food, and feed industries; however, our analysis of the literature showed that, in the l...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-018-1208-0
更新日期:2018-04-01 00:00:00
abstract::The Trichoderma harzianum l-methioninase was purified 7.15-fold with a recovery of 47.9% and the specific activity of 74.4 U/mg of protein. The purified enzyme has an apparent molecular mass of 48 kDa on SDS-PAGE and exhibited maximum activity at pH 8 and 35 °C. The enzyme was catalytically stable below 50 °C and at a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02494-w
更新日期:2020-11-01 00:00:00
abstract::A bacterial strain named XHY-12 was isolated from corn soil samples and identified as Burkholderia sp. based on 16S rDNA sequencing, it displayed high antagonistic activity against 12 fungal pathogens and the common fungal contaminant in grain Aspergillus flavus. Plate experiment showed that XHY-12 fermentation broth ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2112-y
更新日期:2020-04-01 00:00:00
abstract::In this study, analytical profiling of the bevacizumab (BVZ) biosimilars (N = 3) approved in India were evaluated for charge heterogeneity, isoelectric focusing, aggregation and in vitro potency analysis. The charge variants were characterized using high performance cation-exchange chromatography (CEX-HPLC), capillary...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02506-9
更新日期:2020-12-01 00:00:00
abstract::Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1213-3
更新日期:2018-04-01 00:00:00
abstract::Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purificati...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0495-6
更新日期:2016-12-01 00:00:00
abstract::The effects of corn steep liquor (CSL) on hydrocarbon degradation and microbial community structure and function was evaluated in field-moist soil microcosms. Chronically polluted soil treated with CSL (AB4) and an untreated control (3S) was compared over a period of 6 weeks. Gas chromatographic fingerprints of residu...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1580-4
更新日期:2019-02-01 00:00:00
abstract::In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 776...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-020-2070-4
更新日期:2020-02-01 00:00:00
abstract::This is the first study reporting whole genome sequences of two CHIKV strains (KJ679577 and KJ679578) isolated from Eastern Indian patients sera during 2010-2011 outbreak, both of which were of ECSA genotype, but from different subgroups: Indian Ocean outbreak and ECSA subtypes. Furthermore, viral sequences were analy...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1339-3
更新日期:2018-07-01 00:00:00
abstract::One of the most important rapidly emerging mosquito-borne alphavirus is Chikungunya virus (CHIKV). There is a necessity to develop anti-CHIKV therapeutics, as neither antiviral drug nor vaccines have been licensed yet. Several CHIKV proteins are being studied worldwide, but non-structural protein 3 (nsP3) has been les...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1759-8
更新日期:2019-06-01 00:00:00
abstract::The wound healing process is characterized by varied biological and molecular cascades including inflammation, tissue proliferation, and remodeling phase. To augment and maintain these cascades, an all-natural matrix system is proposed. Biocompatible biopolymers, sodium alginate and gelatin, were employed to prepare m...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02453-5
更新日期:2020-11-01 00:00:00
abstract::An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incub...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2097-6
更新日期:2020-03-01 00:00:00
abstract::In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02397-w
更新日期:2020-09-01 00:00:00
abstract::After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0610-3
更新日期:2017-05-01 00:00:00
abstract::The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1489-3
更新日期:2018-11-01 00:00:00
abstract::An efficient regeneration system via a combined pathway of organogenesis and somatic embryogenesis was developed for date palm (Phoenix dactylifera L.) cv. Mejhoul. Adventitious buds were obtained from shoot-tip explants with a frequency of 53.3% after 9 months of culture: 6 months on half-strength Murashige and Skoog...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1235-x
更新日期:2018-04-01 00:00:00
abstract::In this study, we determined using NIRS the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a CNDH population derived from a cross between Cheongcheong and Nagdong rice varieties. The results revealed a higher heritability percentage for the amylose content and comp...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0687-8
更新日期:2017-05-01 00:00:00