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Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13.

Abstract：

:Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% Na2HPO4, pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud's dextrose medium. Results indicate the potential of A. flavipes HN4-13 in the large-scale production of questin through fermentation.

journal_name

3 Biotech

journal_title

3 Biotech

authors

Guo L,Wang L,Li X,Xu X,Guo J,Wang X,Yang W,Xu F,Li F

doi

10.1007/s13205-020-2067-z

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

54

issue

2

eissn

2190-572X

issn

2190-5738

pii

2067

journal_volume

10

pub_type

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