Molecular cloning and functional analysis of a Δ12-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.

Abstract:

:Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ12-fatty acid desaturase (FAD)-encoding gene (Δ 12 CiFAD), 1269 bp in size, was cloned from Chlamydomonas sp. ICE-L. Bioinformatics analysis showed that Δ 12 CiFAD-encoded protein was homologous to known FADs with conserved histidine motifs, and localized to the chloroplast. Functional analysis of Δ 12 CiFAD indicated that recombinant Synechococcus 6803 expressing Δ12CiFAD could accumulate C18:2, whereas recombinant Saccharomyces cerevisiae expressing this enzyme could not accumulate C18:2 or any other new fatty acids. These results indicate that Δ12CiFAD is a functional enzyme in the chloroplast that can adjust Chlamydomonas sp. ICE-L cell membrane fluidity to adapt to Antarctic extreme low-temperature environments, which give us insights into the frigostable and cold-resistant mechanisms of hypothermic organisms.

journal_name

3 Biotech

journal_title

3 Biotech

authors

He Y,Zheng Z,An M,Chen H,Qu C,Liu F,Wang Y,Miao J,Hou X

doi

10.1007/s13205-019-1858-6

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

328

issue

9

eissn

2190-572X

issn

2190-5738

pii

1858

journal_volume

9

pub_type

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