Abstract：
:After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl acetate extract of strain MCCB 248 and stained with Hoechst 33342 showed clear signs of apoptosis, including shrinkage of the cell nucleus, DNA fragmentation and chromatin condensation. Further to this treated cells showed indications of early apoptotic cell death, including a significant proportion of Annexin V positive staining and evidence of DNA damage as observed in the TUNEL assay. Amplified PKS 1 and NRPS genes involved in secondary metabolite production showed only 82% similarity to known biosynthetic genes of Streptomyces, indicating the likely production of a novel secondary metabolite in this extract. Additionally, chemical dereplication efforts using LC-MS/MS molecular networking suggested the presence of a series of undescribed tetraene polyols. Taken together, these results revealed that this Arctic S. artemisiae strain MCCB 248 is a promising candidate for natural products drug discovery and genome mining for potential anticancer agents.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Dhaneesha M,Benjamin Naman C,Krishnan KP,Sinha RK,Jayesh P,Joseph V,Bright Singh IS,Gerwick WH,Sajeevan TPdoi
10.1007/s13205-017-0610-3subject
Has Abstractpub_date
2017-05-01 00:00:00pages
32issue
1eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-017-0610-3journal_volume
7pub_type
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