Abstract:
:Metatranscriptomics, a subset of metagenomics, provides valuable information about the whole gene expression profiling of complex microbial communities of an ecosystem. Metagenomic studies mainly focus on the genomic content and identification of microbes present within a community, while metatranscriptomics provides the diversity of the active genes within such community, their expression profile and how these levels change due to change in environmental conditions. Metatranscriptomics has been applied to different types of environments, from the study of human microbiomes, to those found in plants, animals, within soils and in aquatic systems. Metatranscriptomics, based on the utilization of mRNA isolated from environmental samples, is a suitable approach to mine the eukaryotic gene pool for genes of biotechnological relevance. Also, it is imperative to develop different bioinformatic pipelines to analyse the data obtained from metatranscriptomic analysis. In the present review, we summarise the metatranscriptomics applied to soil environments to study the functional diversity, and discuss approaches for isolating the genes involved in organic matter degradation and providing tolerance to toxic metals, role of metatranscriptomics in microbiome research, various bioinformatics pipelines used in data analysis and technical challenges for gaining biologically meaningful insight of this approach.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Mukherjee A,Reddy MSdoi
10.1007/s13205-020-2057-1subject
Has Abstractpub_date
2020-02-01 00:00:00pages
71issue
2eissn
2190-572Xissn
2190-5738pii
2057journal_volume
10pub_type
杂志文章,评审相关文献
3 Biotech文献大全abstract::Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0894-3
更新日期:2017-08-01 00:00:00
abstract::Advancement in nanotechnology has improved ways for large-scale production and characterization of nanoparticles of physiologically important metals. The current study explores the impact of Zinc Oxide Nanoparticles (ZnO-NP) and Chitosan-Zinc oxide nano-bioformulation (CH-ZnO) in tissue culture raised callus of Nicoti...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02469-x
更新日期:2020-11-01 00:00:00
abstract::The study investigates the ability of methanolic extract of Andrographis paniculata (MAP) to supplement polyvalent anti-snake venom (ASV) in inhibiting neurotoxic enzyme acetylcholinesterase (AChE) and 'spreading factor' hyaluronidase from Naja naja (N.N) venom. AChE and hyaluronidase activity were measured in 100 or ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02462-4
更新日期:2020-11-01 00:00:00
abstract::Kalopanax septemlobus is a medicinal woody species of the family Araliaceae, and the pharmaceutical properties of saponins obtained from K. septemlobus suggest that K. septemlobus has the potential to be a crude drug and dietary health supplement. In this study, we established cell suspension culture of K. septemlobus...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1090-9
更新日期:2018-01-01 00:00:00
abstract::A novel polypyrrole nanowires coated by graphene oxide (PPy-NWs/GO) has been successfully synthesized by one-step electrochemical method, whose structure was different from previously reported PPy/GO composites. The microbial fuel cell equipped with PPy-NWs/GO as anode was fabricated and compared with PPy-NWs ones. Th...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1321-0
更新日期:2018-08-01 00:00:00
abstract::Plant growth-promoting fungi play an important role in development of sustainable agriculture. In the current study, 13 fungal strains were isolated from the rhizosphere of healthy Triticum aestivum (wheat) plant and screened for their indolic auxin production potential. Aspergillus flavus strain PGFW, Aspergillus nig...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1428-3
更新日期:2018-10-01 00:00:00
abstract::In the presented research the extracellular chitinase of Stenotrophomonas rhizophila G22 was biochemically and molecularly characterized. The studied enzyme was purified from a 72-h bacterial culture about 14 times, with a recovery of 63%. The molecular weight of the purified protein was estimated at 50 kDa by SDS-PAG...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-2007-y
更新日期:2020-01-01 00:00:00
abstract::2,4-dinitrotoluene (2,4-DNT) is a priority environmental xenobiotic pollutant which has toxic, mutagenic, and carcinogenic properties. Thus, its biodegradation by applying recent approaches such as taking advantage of plant-bacteria interactions is crucial. In this work, the genes from Burkholderia sp. R34, necessary ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1933-z
更新日期:2019-11-01 00:00:00
abstract::Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0729-2
更新日期:2017-06-01 00:00:00
abstract::Fungal pathogen, Tilletia indica, the cause of Karnal bunt disease in wheat, is severely affecting the yield and grain quality, worldwide. Thus, strict quarantine regulations by most wheat growing countries have to be followed, leading to trade barriers for wheat export. The conventional methods being used for pathoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02191-8
更新日期:2020-05-01 00:00:00
abstract::Coccinia mosaic Virdhunagar virus (KY860899), Tomato leaf curl New Delhi virus (KY860898) and Tomato leaf curl Virdhunagar alphasatellite (KY848691) were found to be associated with leaf curl disease in Momordica charantia (bitter gourd). The complete nucleotide sequence of Coccinia mosaic Virdhunagar virus showed 82%...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-019-1775-8
更新日期:2019-06-01 00:00:00
abstract::In the current study, the production of novel glutaminase free l-asparaginase from a new microbial source (Pseudomonas resinovorans IGS-131) is reported. Optimization of l-asparaginase production using conventional and statistical optimization techniques resulted in an enzyme yield of 37.63 IU/mL, which was 3.45-fold ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2135-4
更新日期:2020-04-01 00:00:00
abstract::To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and their mRNA expression profiles were evaluated using quantitative real-time...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1388-7
更新日期:2018-08-01 00:00:00
abstract::Novel coronavirus disease 2019 (COVID-19) is a positive-sense single-stranded RNA virus which belongs to the Coronaviridae family. COVID-19 outbreak became evident after the severe acute respiratory syndrome coronavirus and the Middle East respiratory syndrome coronavirus in the twenty-first century as the start of th...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-021-02657-3
更新日期:2021-02-01 00:00:00
abstract::Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0552-1
更新日期:2016-12-01 00:00:00
abstract::New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-011-0010-z
更新日期:2011-09-01 00:00:00
abstract::One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was clo...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1285-0
更新日期:2018-05-01 00:00:00
abstract::Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02413-z
更新日期:2020-10-01 00:00:00
abstract::The molecular physiology of milk production of two important dairy species; Sahiwal cows (Bos indicus) and Murrah buffaloes (Bubalus bubalis) are not fully understood due to constraints in obtaining mammary tissue samples because of sacred and ethical reasons. The present study suggests the use of milk-derived mammary...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1642-7
更新日期:2019-03-01 00:00:00
abstract::We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0150-4
更新日期:2014-06-01 00:00:00
abstract::The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1489-3
更新日期:2018-11-01 00:00:00
abstract::In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 776...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-020-2070-4
更新日期:2020-02-01 00:00:00
abstract::A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5-1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0134-4
更新日期:2014-04-01 00:00:00
abstract::Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0594-4
更新日期:2017-05-01 00:00:00
abstract::The emergence of new strains of Magnaporthe oryzae (M. oryzae) is associated with recurrent failure of resistance response mediated by single resistance (R) gene in rice. Therefore, stacking or combining of multiple R genes could improve the durability of resistance against multiple strains of M. oryzae. To achieve th...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1062-5
更新日期:2018-01-01 00:00:00
abstract::Although endophytic bacteria are known to colonize Panax ginseng, little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-018-1417-6
更新日期:2018-09-01 00:00:00
abstract::Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of Tribulus terrestris was screened upon chromoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1695-7
更新日期:2019-04-01 00:00:00
abstract::Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1213-3
更新日期:2018-04-01 00:00:00
abstract::Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0109-x
更新日期:2013-12-01 00:00:00
abstract::Hyaluronic acid (HA) is a biopolymer used in several industries. There is increasing global demand. HA is normally produced on a large scale using attenuated strains of group C streptococci that are pathogenic and fastidious. Accordingly, it is of interest to use a "generally recognized as safe" (GRAS) organism such a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1749-x
更新日期:2019-06-01 00:00:00