Abstract:
:Bioflocculants are secondary metabolites produced by microorganisms during their growth which have received attentions due to their biodegradability, innocuousness and lack of secondary pollution from degradation intermediates. This study reports on a bioflocculant produced by Bacillus specie isolated from Thyume River in South Africa. The bacterial isolate was identified through 16S rDNA sequencing and the BLAST analysis of the nucleotide sequences revealed 99% similarity to Bacillus sp. BCT-7112. The sequence was subsequently deposited in the GenBank as Bacillus sp. AEMREG4 with accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size 4% (v/v) (80%) and starch (81%) as well as yeast extract (82%) as sole carbon and nitrogen sources, respectively. Addition of Ca2+ greatly enhanced the flocculating activity (76%) of crude bioflocculant over a wide range of pH 4-10 and retained high flocculating activity when heated at 100 °C for 1 h. Chemical analyses of the purified bioflocculant revealed carbohydrate (79% w/w) as a predominant component followed by uronic acid (15% w/w) and protein (5% w/w). Fourier transform infrared spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional groups responsible for flocculation and the high flocculation activity achieved portends its industrial applicability.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Ntsangani N,Okaiyeto K,Uchechukwu NU,Olaniran AO,Mabinya LV,Okoh AIdoi
10.1007/s13205-017-0695-8subject
Has Abstractpub_date
2017-05-01 00:00:00pages
78issue
1eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-017-0695-8journal_volume
7pub_type
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