Abstract:
:Conjugated Linoleic Acid (CLA), a fatty acid with high nutraceutical value is produced in rumen by resident bacterial species, especially Butyrivibrio spp. The present study was undertaken to examine the diversity of indigenous Butyrivibrio spp. from rumen liquor of Indian ruminants. The isolates were screened for their CLA production capability at different level of linoleic acid (LA) (0, 200, 400, 600, 800 μg/ml) at different time intervals (0, 2, 4, 6, 12, and 24 h). A total of more than 300 anaerobic cultures were isolated and 31 of them were identified as Butyrivibrio spp. based on morphological, biochemical and molecular characterization. Further, molecular characterization revealed that a large portion (67.7 %) of isolated Butyrivibrio belonged to Butyrivibrio fibrisolvens (B. fibrisolvens) species which is considered to be the most active bacteria amongst the rumen bacteria populace in terms of CLA production. Bacterial isolate VIII (strain 4a) showed highest CLA production ability (140.77 μg/ml) when incubated at 200 μg/ml LA for 2 h, which is 240 % higher than the isolate XXVII, Butyrivibrio proteoclasticus (B. proteoclasticus) showing lowest CLA production (57.28 μg/ml) amongst the screened isolates. It was evident from the observations recorded during the course of experiments that CLA production ability is strain specific and thus did not follow a single pattern. CLA production also varied with time of incubation and concentration of free linoleic acid supplemented in the growth medium. The results of these findings put forward a strain that is high CLA producer and can be further exploited as an additive for enhancing meat and milk quality in ruminants.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Hussain SKA,Srivastava A,Tyagi A,Shandilya UK,Kumar A,Kumar S,Panwar S,Tyagi AKdoi
10.1007/s13205-016-0401-2subject
Has Abstractpub_date
2016-06-01 00:00:00pages
90issue
1eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-016-0401-2journal_volume
6pub_type
杂志文章相关文献
3 Biotech文献大全abstract::We present here a modified, sonication-assisted transient transformation assay for rapid analysis of cis-regulatory elements. We tested promoter elements from MIR159B locus of Brassica juncea by generating stable transgenic lines and compared the transcriptional activity of GUS reporter with that of the transient assa...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1219-x
更新日期:2018-04-01 00:00:00
abstract::Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0894-3
更新日期:2017-08-01 00:00:00
abstract::Isolation of high-quality RNA from weed plants such as Parthenium hysterophorus is a difficult task due to the hindrance caused by numerous secondary metabolites. Such metabolites not only affect the quality and yield of RNA, but also limit the quality of downstream applications. Therefore, the present study was under...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1003-3
更新日期:2017-12-01 00:00:00
abstract::Selection of the raw material and its efficient utilization are the critical factors in economization of second generation (2G) ethanol production. Fermentation of the released sugars into ethanol by a suitable ethanol producing microorganism using cheap media ingredients is the cornerstone of the overall process. Thi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0098-9
更新日期:2013-10-01 00:00:00
abstract::The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was inves...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0795-5
更新日期:2017-07-01 00:00:00
abstract::Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0109-x
更新日期:2013-12-01 00:00:00
abstract::The purpose of this review is to assist readers in understanding the importance of Lupinus albescens to nature, farmers, and scientists. L. albescens is mostly found in Argentina, Uruguay, Paraguay, and in "Campanha, Litoral and Missões" regions of State of Rio Grande do Sul (Brazil). Therefore, this review presents i...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-018-1474-x
更新日期:2018-10-01 00:00:00
abstract::The emergence of new strains of Magnaporthe oryzae (M. oryzae) is associated with recurrent failure of resistance response mediated by single resistance (R) gene in rice. Therefore, stacking or combining of multiple R genes could improve the durability of resistance against multiple strains of M. oryzae. To achieve th...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1062-5
更新日期:2018-01-01 00:00:00
abstract::New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-011-0010-z
更新日期:2011-09-01 00:00:00
abstract::We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0150-4
更新日期:2014-06-01 00:00:00
abstract::Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2053-5
更新日期:2020-02-01 00:00:00
abstract::To assess the genetic diversity among four canola cultivars (namely, Serw-3, Serw-4, Misser L-16 and Semu 249), random amplified polymorphic DNA (RAPD), simple sequence repeat polymorphism (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed. The data indicated that all of the three molecula...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0165-x
更新日期:2014-08-01 00:00:00
abstract::In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate fo...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1983-2
更新日期:2019-12-01 00:00:00
abstract::Plant growth-promoting fungi play an important role in development of sustainable agriculture. In the current study, 13 fungal strains were isolated from the rhizosphere of healthy Triticum aestivum (wheat) plant and screened for their indolic auxin production potential. Aspergillus flavus strain PGFW, Aspergillus nig...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1428-3
更新日期:2018-10-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most common non-skin cancer in the world. Tobacco chewing is implicated with most of the cases of HNSCC but this type of cancer is increasing in non-tobacco chewers as well. This study was instigated to provide comprehensive variant and gene-level data in HNSC...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-014-0267-0
更新日期:2015-10-01 00:00:00
abstract::The different developmental stage-associated microbiota of the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae), was characterized using 16S rRNA gene (V3-V4 region) metabarcoding on the Illumina HiSeq platform. Taxonomically, at 97% similarity, there were total 16 bacterial phyla, comprising of 24 classes, 5...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02381-4
更新日期:2020-09-01 00:00:00
abstract::Bio-inoculants play an important role for sustainable agriculture. Application of nanocompounds in the agriculture sector provides strength and is reported to enhance crop production but the combined effect of nanocompounds and plant growth-promoting rhizobacteria on plants has not been studied much. Therefore, the pr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02561-2
更新日期:2021-01-01 00:00:00
abstract::2,4-dinitrotoluene (2,4-DNT) is a priority environmental xenobiotic pollutant which has toxic, mutagenic, and carcinogenic properties. Thus, its biodegradation by applying recent approaches such as taking advantage of plant-bacteria interactions is crucial. In this work, the genes from Burkholderia sp. R34, necessary ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1933-z
更新日期:2019-11-01 00:00:00
abstract::The aim of the present study was to assess the arsenic (As) transformation potential of endophytic bacteria isolated from roots of Pteris vittata plant. The endophytic bacterium was tested for minimal inhibitory concentration (MIC) against As. The endophytic strain RPT exhibited the highest resistance to As(V) (400 mg...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-017-0901-8
更新日期:2017-08-01 00:00:00
abstract::Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0552-1
更新日期:2016-12-01 00:00:00
abstract::In the present study, we have experimentally and theoretically studied the free-radical quenching property of dihydrocanaric acid (DCA) isolated from seedpods of Holarrhena antidysenterica. A modified method was used to estimate the nitric oxide scavenging effect of the DCA (significant activity of 75.22%) along with ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02221-5
更新日期:2020-06-01 00:00:00
abstract::Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast trans...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-015-0315-4
更新日期:2016-06-01 00:00:00
abstract::In this study, the Ion Torrent PGM platform was employed to conduct a whole-genome sequencing analysis of Eugenia uniflora L., aiming to generate new genomic information for this non-model species. The whole-genome set of assembled sequences (WGSAS) generated 2601 contigs, with a cumulative length of 3.15 Mbp. About 2...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-019-1729-1
更新日期:2019-05-01 00:00:00
abstract::RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predict...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1893-3
更新日期:2019-10-01 00:00:00
abstract::Actinomycetes strain was isolated from leaf litter soil sample and was identified as Streptomyces sp. by conventional and molecular approaches. The biologically active compound responsible for antimicrobial and anticancer activity of the strain JAR6 was elucidated by solid state fermentation followed by subsequent chr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-1044-7
更新日期:2018-01-01 00:00:00
abstract::Nine (9) different date palm (Phoenix dactylifera L.) cultivars from UAE, which differ in their flower timings were selected to determine the polymorphism and genetic relationship between these cultivars. Hereditary differences and interrelationships were assessed utilizing inter-simple sequence repeat (ISSR) and dire...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1108-3
更新日期:2018-02-01 00:00:00
abstract::The tolerance of the pentose-fermenting yeast Meyerozyma guilliermondii to the inhibitors released after the biomass hydrolysis, such as acetic acid and furfural, was surveyed. We first verified the effects of acetic acid and cell concentrations and initial pH on the growth of a M. guilliermondii strain in a semi-synt...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1143-0
更新日期:2018-02-01 00:00:00
abstract::Glycoprotein D (gD) of Herpes Simplex Virus-2 is used as an antigen in various anti-herpes subunit vaccines owing to its involvement in binding the host cell receptors for host infectivity. However, most of these monomeric protein based candidates have shown low immunogenicity in animal models. To enhance the immunoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02452-6
更新日期:2020-11-01 00:00:00
abstract::We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0365-2
更新日期:2016-06-01 00:00:00
abstract::Viral diseases affecting sweetpotato are the most devastating and cause up to 98 % yield loss. In this paper, we report, meristem culture, graft transmission and virus indexing for management of viral pathogens in seven elite sweetpotato cultivars. Plantlets were developed in vitro from the apical meristematic dome wi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0080-6
更新日期:2013-04-01 00:00:00