Abstract：
:The possibility of using Aspergillus terreus protease in detergent formulations was investigated. Sodium dodecyl sulfate (SDS) and native polyacrylamide gel electrophoresis indicated that the purified alkaline protease (148.9 U/mg) is a monomeric enzyme with a molecular mass of 16 ± 1 kDa. This was confirmed by liquid chromatography-mass spectrometry. The active enzyme degraded the co-polymerized gelatin. The protease demonstrated excellent stability at pH range 8.0-12.0 with optimum at pH 11.0. It was almost 100 % stable at 50 °C for 24 h, enhanced by Ca2+ and Mg2+, but inhibited by Hg2+, and strongly inhibited by phenylmethyl sulfonyl fluoride. It showed maximum activity against casein followed by gelatin; its Vmax was 12.8 U/ml with its corresponding KM of 5.4 mg/ml. The proteolytic activity was activated by Tween-80, Triton-100 and SDS, and remained unaltered in the presence of H2O2 and NaClO. The enzyme exhibited higher storage stability at 4, 28 and -20 °C. It was stable and compatible to the desired level in the local detergents. The addition of the protease to the Super wheel improved its blood stain removal. The isolated protease can thus be a choice option in detergent industry.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Niyonzima FN,More SSdoi
10.1007/s13205-014-0200-6subject
Has Abstractpub_date
2015-02-01 00:00:00pages
61-70issue
1eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-014-0200-6journal_volume
5pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:The Trichoderma harzianum l-methioninase was purified 7.15-fold with a recovery of 47.9% and the specific activity of 74.4 U/mg of protein. The purified enzyme has an apparent molecular mass of 48 kDa on SDS-PAGE and exhibited maximum activity at pH 8 and 35 °C. The enzyme was catalytically stable below 50 °C and at a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02494-w
更新日期：2020-11-01 00:00:00
abstract：:This bibliometric-based review analyses the trends in 1010 published articles (1990 to mid-2018) on the high-value application of Chlorella, and illustrates the evolution and latest tendencies at a global level by the number of publications and their distribution, issuing institutions and countries or regions, the sou...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2102-0
更新日期：2020-03-01 00:00:00
abstract：:The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0438-2
更新日期：2016-12-01 00:00:00
abstract：:This paper investigated the effects of parameters, like inoculum size (15, 10 and 5% of the working volume of the reactor), gas velocities (0.0027, 0.00342 and 0.0068 m/s), bed heights (0.3, 0.6 and 0.9 m), static bed heights (4.85 and 2.43 cm), sizes of solid media particles (12, 4 mm), and the height to diameter rat...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1059-0
更新日期：2018-02-01 00:00:00
abstract：:The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K3Fe(CN)-6 as redo...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02315-0
更新日期：2020-07-01 00:00:00
abstract：:Bacillus velezensis LPL-K103, which shows strong antifungal function, was isolated from the surface of lemon in Beijing, China. The complete genome of B. velezensis LPL-K103 contains a circular chromosome of 3,933,292 bp (46.61% G+C content). According to genomic analysis, 4080 protein-coding genes, 113 RNAs (27 rRNAs...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-019-1995-y
更新日期：2020-01-01 00:00:00
abstract：:We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0150-4
更新日期：2014-06-01 00:00:00
abstract：:The prolactin receptor (PRLR), a type I cytokine receptor, must bind prolactin (PRL) to act on target cells to mediate various physiological functions, including reproduction and lactation. This study identified an 80-bp insertion/deletion (indel) polymorphism in the 3'-untranslated region (3'-UTR) of the chicken PRLR...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1827-0
更新日期：2019-08-01 00:00:00
abstract：:In the present study, we fabricated epigallocatechin-3-gallate (EGCG) loaded albumin nanoparticles (Alb-NP-EGCG) to enhance bioavailability and improve pharmacokinetic parameters of EGCG. The physicochemical properties of the Alb-NP-EGCG were studied using scanning electron microscopy, differential scanning calorimetr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1772-y
更新日期：2019-06-01 00:00:00
abstract：:Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. T...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-016-0506-7
更新日期：2016-12-01 00:00:00
abstract：:Hyaluronic acid (HA) is a biopolymer used in several industries. There is increasing global demand. HA is normally produced on a large scale using attenuated strains of group C streptococci that are pathogenic and fastidious. Accordingly, it is of interest to use a "generally recognized as safe" (GRAS) organism such a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1749-x
更新日期：2019-06-01 00:00:00
abstract：:Salmonella typhimurium (S. typhimurium) represents an important global public health problem and has the ability to survive under desiccation conditions in foods and food processing facilities for years. The aim of this study was to investigate the effects of Allium sativum (A. sativum) and Cuminum cyminum (C. cyminum...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02286-2
更新日期：2020-07-01 00:00:00
abstract：:Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0788-4
更新日期：2017-07-01 00:00:00
abstract：:Gastric cancer is one of the most common cancers in modern societies. Previous studies have shown that the use of nanoparticle complexes is effective in the treatment of cancer. The aim of this study was to investigate the cytotoxicity and anticancer properties of cobalt oxide (Co3O4) nanoparticles (NPs) functionalize...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02230-4
更新日期：2020-05-01 00:00:00
abstract：:Parthenium hysterophorus is a noxious weed in America, Asia, Africa and Australia. This weed is considered to be a cause of allergic respiratory problems, contact dermatitis, mutagenicity in human and livestock. Crop production is drastically reduced owing to its allelopathy. Also aggressive dominance of this weed thr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0007-7
更新日期：2011-07-01 00:00:00
abstract：:Graminaceous plants secrete hydroxylated phytosiderophores encoded by the genes iron-deficiency-specific clone 2 (Ids2) and iron-deficiency-specific clone 3 (Ids3). An effort was made to identify a putative ortholog of Hodeum vulgare Ids3 gene in hexaploid wheat. The protein structure of TaIDS3 was modeled using homol...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1230-2
更新日期：2018-04-01 00:00:00
abstract：:Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was select...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0764-z
更新日期：2017-06-01 00:00:00
abstract：:The present study was conducted to investigate the antileishmanial activity of biogenic silver nanoparticles (AgNPs) compared to chemically synthesized AgNPs. A nano dimension size (10-15 nm) biogenic AgNPs was produced and characterized by UV-Vis spectroscopy and X-rays diffraction. The chemically synthesized AgNPs w...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1121-6
更新日期：2018-02-01 00:00:00
abstract：:In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) con...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02359-2
更新日期：2020-08-01 00:00:00
abstract：:The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-st...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0213-1
更新日期：2015-04-01 00:00:00
abstract：:We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars 'Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both culti...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1408-7
更新日期：2018-09-01 00:00:00
abstract：:Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02413-z
更新日期：2020-10-01 00:00:00
abstract：:In this work, Thauera sp. DO isolated from sludge and sediment utilized p-chlorocresol and some related compounds as the sole carbon and energy sources under both aerobic and anaerobic conditions. The pathways for p-chlorocresol in the isolate under each condition were different. Under the aerobic condition, p-chloroc...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-2025-9
更新日期：2020-02-01 00:00:00
abstract：:The wound healing process is characterized by varied biological and molecular cascades including inflammation, tissue proliferation, and remodeling phase. To augment and maintain these cascades, an all-natural matrix system is proposed. Biocompatible biopolymers, sodium alginate and gelatin, were employed to prepare m...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02453-5
更新日期：2020-11-01 00:00:00
abstract：:The aim of this study was to explore the neuroprotective effect and the underlying mechanism of erythropoietin (EPO) on the cortical neuronal cells insulted with oxygen and glucose deprivation (OGD). Different concentrations of EPO were used to determine the anti-apoptosis effect of EPO. In addition, PI3K inhibitor LY...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1667-y
更新日期：2019-04-01 00:00:00
abstract：:The effects of corn steep liquor (CSL) on hydrocarbon degradation and microbial community structure and function was evaluated in field-moist soil microcosms. Chronically polluted soil treated with CSL (AB4) and an untreated control (3S) was compared over a period of 6 weeks. Gas chromatographic fingerprints of residu...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1580-4
更新日期：2019-02-01 00:00:00
abstract：:Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoi...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0729-2
更新日期：2017-06-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is the sixth most common non-skin cancer in the world. Tobacco chewing is implicated with most of the cases of HNSCC but this type of cancer is increasing in non-tobacco chewers as well. This study was instigated to provide comprehensive variant and gene-level data in HNSC...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0267-0
更新日期：2015-10-01 00:00:00
abstract：:Metatranscriptomics, a subset of metagenomics, provides valuable information about the whole gene expression profiling of complex microbial communities of an ecosystem. Metagenomic studies mainly focus on the genomic content and identification of microbes present within a community, while metatranscriptomics provides ...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-020-2057-1
更新日期：2020-02-01 00:00:00
abstract：:Biological methods of nanoparticles synthesis are ecologically sound and sustainable alternative to the conventional methods. On the basis of aforesaid premise, the present study deals with the optimization and fabrication of gold nanoparticles (Au-NPs) using easily available bio-resource, Delonix regia leaf extract. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0432-8
更新日期：2016-12-01 00:00:00
