Abstract:
:The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K3Fe(CN)-6 as redox indicators. The selectivity of the developed biosensor was demonstrated using complementary and mismatch oligonucleotide sequences. The sensitivity (S) of the developed sensor was recorded as 3461 (μA/cm2)/ng and limit of detection (LOD) was found to be 82 fg/6 µl with the regression coefficient (R2) 0.941 using CV. The sensor was characterized by field emission scanning electron microscopy (FE-SEM). The electrode was found to be stable for six months, with only 10% loss in the initial CV current.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Saini K,Kaushal A,Gupta S,Kumar Ddoi
10.1007/s13205-020-02315-0subject
Has Abstractpub_date
2020-07-01 00:00:00pages
327issue
7eissn
2190-572Xissn
2190-5738pii
2315journal_volume
10pub_type
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