Abstract:
:The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components influencing the enzyme production were identified using a two-level fractional factorial Plackett-Burman design. Among the various variables screened, starch, ammonium sulphate and calcium chloride were found to be most significant medium components. The optimum levels of these significant parameters were determined employing the response surface Central Composite design which significantly increased the enzyme production with the supplementation of starch 0.01 g, ammonium sulphate 0.2 g and 5 mM calcium chloride in the production medium. Temperature and pH stability of the alpha-amylase suggested its wide application in the food and pharmaceutical industries.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Prajapati VS,Trivedi UB,Patel KCdoi
10.1007/s13205-014-0213-1subject
Has Abstractpub_date
2015-04-01 00:00:00pages
211-220issue
2eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-014-0213-1journal_volume
5pub_type
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