Abstract:
:In this study, we report the isolation and characterization of the mRNA encoding OPAQUE2 (O2) like TF of finger millet (FM) (Eleusine coracana) (EcO2). Full-length EcO2 mRNA was isolated using conserved primers designed by aligning O2 mRNAs of different cereals followed by 3' and 5' RACE (Rapid Amplification of cDNA Ends). The assembled full-length EcO2 mRNA was found to contain an ORF of 1248-nt coding the 416 amino acids O2 protein. Domain analysis revealed the presence of the BLZ and bZIP-C domains which is a characteristic feature of O2 proteins. Phylogenetic analysis of EcO2 protein with other bZIP proteins identified using finger millet transcriptome data and O2 proteins of other cereals showed that EcO2 shared high sequence similarity with barley BLZ1 protein. Transcripts of EcO2 were detected in root, stem, leaves, and seed development stages. Furthermore, to investigate nitrogen responsiveness and the role of EcO2 in regulating seed storage protein gene expression, the expression profiles of EcO2 along with an α-prolamin gene were studied during the seed development stages of two FM genotypes (GE-3885 and GE-1437) differing in grain protein content (13.8 and 6.2%, respectively) grown under increasing nitrogen inputs. Compared to GE-1437, the EcO2 was relatively highly expressed during the S2 stage of seed development which further increased as nitrogen input was increased. The Ecα-prolamin gene was strongly induced in the high protein genotype (GE-3885) at all nitrogen inputs. These results indicate the presence of nitrogen responsiveness regulatory elements which might play an important role in accumulating protein in FM genotypes through modulating EcO2 expression by sensing plant nitrogen status.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Gaur VS,Kumar L,Gupta S,Jaiswal JP,Pandey D,Kumar Adoi
10.1007/s13205-018-1150-1subject
Has Abstractpub_date
2018-03-01 00:00:00pages
163issue
3eissn
2190-572Xissn
2190-5738pii
1150journal_volume
8pub_type
杂志文章相关文献
3 Biotech文献大全abstract::In fishes, trypsins are adapted to different environmental conditions, and the biochemical and kinetic properties of a broad variety of native isoforms have been studied. Proteolytic enzymes remain in high demand in the detergent, food, and feed industries; however, our analysis of the literature showed that, in the l...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-018-1208-0
更新日期:2018-04-01 00:00:00
abstract::Pseudomonas aeruginosa infections are a leading cause of death in patients suffering from respiratory diseases. The multidrug-resistant nature of Pseudomonas is potentiated by a process known as quorum sensing. The aim of this study was to reveal new inhibitors of a well-validated but quite unexplored target, enoyl-AC...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1567-1
更新日期:2019-02-01 00:00:00
abstract::We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-013-0150-4
更新日期:2014-06-01 00:00:00
abstract::In this study, a novel and stable gene transformation system was developed under control of Maize Proteinase Inhibitor (MPI) as an inducible promoter using the Mesoporous Silica Nanoparticles (MSNs). The functionalized MSNs with a proper particle size were synthesized and attached to a recombinant construct (pDNA) con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02359-2
更新日期:2020-08-01 00:00:00
abstract::In the present study, we have experimentally and theoretically studied the free-radical quenching property of dihydrocanaric acid (DCA) isolated from seedpods of Holarrhena antidysenterica. A modified method was used to estimate the nitric oxide scavenging effect of the DCA (significant activity of 75.22%) along with ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02221-5
更新日期:2020-06-01 00:00:00
abstract::In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1971-6
更新日期:2019-11-01 00:00:00
abstract::A detailed understanding of the fate of xenobiotics introduced into the environment and the mechanisms involved in their biotransformation, biodegradation, and biosorption is essential to improve the efficiency of remediation techniques. Mycoremediation is a form of bioremediation technique that has become increasingl...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02177-6
更新日期:2020-04-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most common non-skin cancer in the world. Tobacco chewing is implicated with most of the cases of HNSCC but this type of cancer is increasing in non-tobacco chewers as well. This study was instigated to provide comprehensive variant and gene-level data in HNSC...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-014-0267-0
更新日期:2015-10-01 00:00:00
abstract::Cytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~ 21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. To dissect the function of miRNAs in soybean CMS, a total...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1543-1
更新日期:2019-01-01 00:00:00
abstract::We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0365-2
更新日期:2016-06-01 00:00:00
abstract::The emerging SARS-CoV-2 viral disease (COVID-19) has caused a global health alert due to its high rate of infection and mortality in individuals with chronic cardiovascular comorbidities, in addition to generating complex clinical conditions. This has forced the scientific community to explore different strategies tha...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-020-02619-1
更新日期:2021-02-01 00:00:00
abstract::Present study mainly aimed to ascertain the distribution characteristics of gene-based microsatellite loci and to develop polymorphic SSR markers from the already available transcriptome data of Elaeis guineensis Jacq, an important oil crop. From this study, we identified the sum of 5791 SSRs across 51,425 unigenes fr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02269-3
更新日期:2020-06-01 00:00:00
abstract::In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 776...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-020-2070-4
更新日期:2020-02-01 00:00:00
abstract::Production of tannase from B. gottheilii M2S2 was studied under solid-state fermentation with an optimized medium consisting of polyurethane foam matrix of dimension 40 × 40 × 5 mm, impregnated with a liquid medium comprising (w/v): 4% tannic acid; 2% NH4NO3; 0.1% KH2PO4; 0.2% MgSO4; 0.1% NaCl and 0.05% CaCl2·2H2O in ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0909-0
更新日期:2017-10-01 00:00:00
abstract::Environmental conditions during the in vitro stages of a micropropagation process can further affect the ex vitro plant performance. In this work, the behaviour of four blue cultivars of statice (Limonium sinuatum) was studied with respect to four distances (18.0, 12.5, 8.0, or 2.0 cm) between culture tubes and the li...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-018-1367-z
更新日期:2018-08-01 00:00:00
abstract::Electroporation parameters can be optimized by coupling RC constant values with the amount of electrical power dissipation in the electroporation medium. Electroporation efficiency increases more steeply with power at low power values. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-012-0105-1
更新日期:2013-08-01 00:00:00
abstract::Understanding the molecular mechanism underlying photoperiod sensitivity will play a crucial role in extending the cropping area of Cajanus cajan, a photoperiod sensitive major grain legume of India and Africa. In flowering plants, Flowering locus T (FT) gene is involved in the production of florigen molecule which is...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02180-x
更新日期:2020-05-01 00:00:00
abstract::We sequenced the entire tb1 gene in six maize inbreds and its wild relatives (parviglumis, mexicana, perennis and luxurians) to characterize it at molecular level. Hopscotch and Tourist transposable elements were observed in the upstream of tb1 in all maize inbreds, while they were absent in wild relatives. In maize, ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-2052-6
更新日期:2020-02-01 00:00:00
abstract::Bioflocculants are secondary metabolites produced by microorganisms during their growth which have received attentions due to their biodegradability, innocuousness and lack of secondary pollution from degradation intermediates. This study reports on a bioflocculant produced by Bacillus specie isolated from Thyume Rive...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0695-8
更新日期:2017-05-01 00:00:00
abstract::Statistical experimental designs were used to optimize conditions for recombinant Burkholderia pyrrocinia feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal con...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02198-1
更新日期:2020-05-01 00:00:00
abstract::Genome sequence of Paenibacillus polymyxa ND25 isolated from cow rumen is reported for being a potential candidate in hydrolysis of lignocellulosic plant biomass. Draft genome sequence generated 5.73 Mb data containing 4922 putative protein coding genes, of which 140 are annotated for glycoside hydrolases. P. polymyxa...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1274-3
更新日期:2018-05-01 00:00:00
abstract::Thermophilic bacterial communities generate thick biofilm on carbon steel API 5LX and produce extracellular metabolic products to accelerate the corrosion process in oil reservoirs. In the present study, nine thermophilic biocorrosive bacterial strains belonging to Bacillus and Geobacillus were isolated from the crude...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1604-0
更新日期:2019-03-01 00:00:00
abstract::The action of FTO on myoblasts proliferation and differentiation and molecular mechanism underlying it were investigated by transfecting with FTO lentiviral overexpression vector and gene expression profile sequencing. Compared with the control group, myoblasts with FTO transfection was significantly enhanced prolifer...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02386-z
更新日期:2020-09-01 00:00:00
abstract::We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars 'Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both culti...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1408-7
更新日期:2018-09-01 00:00:00
abstract::Phylogenetic relationship of 22 FLPs was revealed on the basis of polymorphism in three genes namely 16S rDNA, Pseudomonas-specific and rpoD gene regions. The primers for 16S rDNA, Pseudomonas-specific region and rpoD gene region were amplifying a region of 1492, 990 and 760 bp, respectively, from all the isolates inv...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0386-x
更新日期:2016-06-01 00:00:00
abstract::Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0788-4
更新日期:2017-07-01 00:00:00
abstract::Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1213-3
更新日期:2018-04-01 00:00:00
abstract::Farnesyl diphosphate synthase (FPPase) is an enzyme that catalyzes the condensation between one molecule of dimethylallyl diphosphate (DMAPP) and two molecules of isopentenyl diphosphate (IPP) to produce farnesyl diphosphate (FPP). FPP is an important precursor in the isoprenoid synthesis pathway. In this study, the c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0792-8
更新日期:2017-08-01 00:00:00
abstract::Several proteins and peptides of microbial origin are reported for their elicitor properties, which play a vital role in the development of local and systemic resistances in plants. In this study, the efficacy of total crude proteins (TCP) extracted from six different Trichoderma spp. (T. asperellum, T. harzianum, T. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0816-4
更新日期:2017-07-01 00:00:00
abstract::Plant growth promoting rhizobacteria are key to soil and plant health maintenance. In the present study, two PGPR strains which were identified as Bacillus spp. (accession number KX650178 and KX650179) with nanozeolite (50 ppm) were applied to the seeds in different combinations and tested on growth profile of maize c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1142-1
更新日期:2018-03-01 00:00:00