Abstract：
:We sequenced the entire tb1 gene in six maize inbreds and its wild relatives (parviglumis, mexicana, perennis and luxurians) to characterize it at molecular level. Hopscotch and Tourist transposable elements were observed in the upstream of tb1 in all maize inbreds, while they were absent in wild relatives. In maize, tb1 consisted of 431-443 bp 5'UTR, 1101 bp coding sequence and 211-219 bp 3'UTR. In promoter region, mutations in the light response element in mexicana (~ 35 bp and ~ 55 bp upstream of TSS) and perennis (at ~ 35 bp upstream of TSS) were found. A 6 bp insertion at 420 bp downstream of the polyA signal site was present among teosinte accessions, while it was not observed in maize. A codominant marker flanking the 6 bp InDel was developed, and it differentiated the teosintes from maize. In Tb1 protein, alanine (12.7-14.6%) was the most abundant amino acid with tryptophan as the rarest (0.5-0.9%). The molecular weight of Tb1 protein was 38757.15 g/mol except 'Palomero Toluqueno' and HKI1128. R and TCP motifs in Tb1 protein were highly conserved across maize, teosinte and orthologues, while TCP domain differed for tb1 paralogue. Tb1 possessed important role in light-, auxin-, stress-response and meristem identity maintenance. Presence of molecular signal suggested its localization in mitochondria, nucleus and nucleolus. Parviglumis and mexicana shared closer relationship with maize than perennis and luxurians. A highly conserved 59-60 amino acids long bHLH region was observed across genotypes. Information generated here assumes significance in evolution of tb1 gene and breeding for enhancement of prolificacy in maize.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Prakash NR,Chhabra R,Zunjare RU,Muthusamy V,Hossain Fdoi
10.1007/s13205-020-2052-6subject
Has Abstractpub_date
2020-02-01 00:00:00pages
77issue
2eissn
2190-572Xissn
2190-5738pii
2052journal_volume
10pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:Genetic polymorphism amid plant species is a crucial factor for plant improvement and maintaining their biodiversity. Evaluation of genetic diversity amongst plant species is significant to deal with the environmental stress conditions and their effective involvement in the breeding programs. Hence, in present study, ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1736-2
更新日期：2019-06-01 00:00:00
abstract：:Cell-free extracts of twenty terrestrial cyanobacteria were evaluated for their antioxidant properties in terms of free-radical scavenging (DPPH and ABTS) and metal chelating activity and deoxyribose protection. Extract of Anabaena constricta was the most prominent antioxidant agent (IC50 for DPPH activity 0.91 mg ml-...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0786-6
更新日期：2017-06-01 00:00:00
abstract：:Farnesyl diphosphate synthase (FPPase) is an enzyme that catalyzes the condensation between one molecule of dimethylallyl diphosphate (DMAPP) and two molecules of isopentenyl diphosphate (IPP) to produce farnesyl diphosphate (FPP). FPP is an important precursor in the isoprenoid synthesis pathway. In this study, the c...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0792-8
更新日期：2017-08-01 00:00:00
abstract：:In this overview, the authors have discussed the potential advantages of the association between mycorrhizae and plants, their mutual accelerated growth under favorable conditions and their role in nutrient supply. In addition, methods for isolating mycorrhizae are described and spore morphologies and their adaptation...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-020-02188-3
更新日期：2020-05-01 00:00:00
abstract：:Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02413-z
更新日期：2020-10-01 00:00:00
abstract：:MicroRNAs-199a-5p (miR-199a-5p) plays critical regulatory roles in various types of human cancers. However, the biological function and regulatory mechanisms of miR-199a-5p in colorectal cancer (CRC) remain unclear. The aim of this study was to investigate the role of miR-199a-5p in CRC and possible mechanisms of its ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02433-9
更新日期：2020-10-01 00:00:00
abstract：:Long-term production in commercial straw biogas plants has been rare in China due to inefficiencies in the logistics stream. Biomass densification could be a potential solution to this issue. Therefore, we conducted a study to evaluate whether biomass densification is a more efficient and sustainable option. We perfor...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0914-3
更新日期：2017-10-01 00:00:00
abstract：:Electroporation parameters can be optimized by coupling RC constant values with the amount of electrical power dissipation in the electroporation medium. Electroporation efficiency increases more steeply with power at low power values. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0105-1
更新日期：2013-08-01 00:00:00
abstract：:The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0682-0
更新日期：2017-05-01 00:00:00
abstract：:The present study was aimed to evaluate the immuno-modulatory effect of Brucella-specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice (n = 6 ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1905-3
更新日期：2019-10-01 00:00:00
abstract：:In this investigation, potential inhibitors of α-amylase, one of the key regulatory enzymes in diabetes were characterized from the methanolic extract of Leucas ciliata Benth. (Lamiaceae), a traditional medicinal plant of the Western Ghats, southern India and the ethyl acetate extract of Streptomyces longisporoflavus ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02547-0
更新日期：2021-02-01 00:00:00
abstract：:A one-step formic acid-catalyzed organosolv process using a low-boiling point acid-solvent system was studied for fractionation of sugarcane bagasse. Compared to H2SO4, the use of formic acid as a promoter resulted in higher efficiency and selectivity on removals of hemicellulose and lignin with increased enzymatic di...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1244-9
更新日期：2018-05-01 00:00:00
abstract：:Infectious diseases caused by antimicrobial-resistant microbes (ARMs) and the treatment are the serious problems in the field of medical science today world over. The development of alternative drug line to treat such infectious diseases is urgently required. Researches on ARMs revealed the presence of membrane protei...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0180-y
更新日期：2014-10-01 00:00:00
abstract：:Emblica officinalis Gaertn. syn. Phyllanthus emblica L., universally known as 'Amla' or 'Aonla' or 'Indian gooseberry', is a popular fruit tree belonging to the family Euphorbiaceae and order Geraniales. It is said to be the very first tree that originated on earth, as claimed by age-old Indian mythology. Almost all p...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-020-02615-5
更新日期：2021-02-01 00:00:00
abstract：:Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0393-y
更新日期：2016-06-01 00:00:00
abstract：:Due to the lack of early diagnosis, cancer remains as one of the leading cause of human mortality. Inability to translate research into clinical trials and also inability of chemotherapeutics delivery to targeted tumor sites are major drawbacks in cancer therapeutics. With the emergence of nanomedicine, several nanopr...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-019-1940-0
更新日期：2019-11-01 00:00:00
abstract：:Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved wit...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1315-y
更新日期：2018-07-01 00:00:00
abstract：:Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoi...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0729-2
更新日期：2017-06-01 00:00:00
abstract：:Environmental conditions during the in vitro stages of a micropropagation process can further affect the ex vitro plant performance. In this work, the behaviour of four blue cultivars of statice (Limonium sinuatum) was studied with respect to four distances (18.0, 12.5, 8.0, or 2.0 cm) between culture tubes and the li...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-018-1367-z
更新日期：2018-08-01 00:00:00
abstract：:The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-st...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0213-1
更新日期：2015-04-01 00:00:00
abstract：:Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purificati...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0495-6
更新日期：2016-12-01 00:00:00
abstract：:A bacterial strain named XHY-12 was isolated from corn soil samples and identified as Burkholderia sp. based on 16S rDNA sequencing, it displayed high antagonistic activity against 12 fungal pathogens and the common fungal contaminant in grain Aspergillus flavus. Plate experiment showed that XHY-12 fermentation broth ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2112-y
更新日期：2020-04-01 00:00:00
abstract：:Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2053-5
更新日期：2020-02-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is the sixth most common non-skin cancer in the world. Tobacco chewing is implicated with most of the cases of HNSCC but this type of cancer is increasing in non-tobacco chewers as well. This study was instigated to provide comprehensive variant and gene-level data in HNSC...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0267-0
更新日期：2015-10-01 00:00:00
abstract：:An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incub...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2097-6
更新日期：2020-03-01 00:00:00
abstract：:Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of coppe...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0929-9
更新日期：2017-10-01 00:00:00
abstract：:Cassava is an important food for millions of people around the world. However, cassava is deficient in protein, iron, zinc, pro-vitamin A and vitamin E. Cassava biofortified with pro-vitamin A can help reduce Vitamin A Deficiency among the undernourished communities that rely upon it for sustenance. BioCassava Plus pr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0243-8
更新日期：2015-08-01 00:00:00
abstract：:Banana bunchy top virus (BBTV) is a circular single-stranded DNA virus with multi-components. The knowledge about interaction between viral proteins and pathogenesis mechanism of BBTV remains unclear. In this study, the coat protein gene (CP, ORF 516 bp) and nuclear shuttle protein gene (NSP, ORF 465 bp) from BBTV B2 ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1656-1
更新日期：2019-04-01 00:00:00
abstract：:Parthenium hysterophorus is a noxious weed in America, Asia, Africa and Australia. This weed is considered to be a cause of allergic respiratory problems, contact dermatitis, mutagenicity in human and livestock. Crop production is drastically reduced owing to its allelopathy. Also aggressive dominance of this weed thr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0007-7
更新日期：2011-07-01 00:00:00
abstract：:Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1213-3
更新日期：2018-04-01 00:00:00
