Abstract：
:We sequenced the entire tb1 gene in six maize inbreds and its wild relatives (parviglumis, mexicana, perennis and luxurians) to characterize it at molecular level. Hopscotch and Tourist transposable elements were observed in the upstream of tb1 in all maize inbreds, while they were absent in wild relatives. In maize, tb1 consisted of 431-443 bp 5'UTR, 1101 bp coding sequence and 211-219 bp 3'UTR. In promoter region, mutations in the light response element in mexicana (~ 35 bp and ~ 55 bp upstream of TSS) and perennis (at ~ 35 bp upstream of TSS) were found. A 6 bp insertion at 420 bp downstream of the polyA signal site was present among teosinte accessions, while it was not observed in maize. A codominant marker flanking the 6 bp InDel was developed, and it differentiated the teosintes from maize. In Tb1 protein, alanine (12.7-14.6%) was the most abundant amino acid with tryptophan as the rarest (0.5-0.9%). The molecular weight of Tb1 protein was 38757.15 g/mol except 'Palomero Toluqueno' and HKI1128. R and TCP motifs in Tb1 protein were highly conserved across maize, teosinte and orthologues, while TCP domain differed for tb1 paralogue. Tb1 possessed important role in light-, auxin-, stress-response and meristem identity maintenance. Presence of molecular signal suggested its localization in mitochondria, nucleus and nucleolus. Parviglumis and mexicana shared closer relationship with maize than perennis and luxurians. A highly conserved 59-60 amino acids long bHLH region was observed across genotypes. Information generated here assumes significance in evolution of tb1 gene and breeding for enhancement of prolificacy in maize.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Prakash NR,Chhabra R,Zunjare RU,Muthusamy V,Hossain Fdoi
10.1007/s13205-020-2052-6subject
Has Abstractpub_date
2020-02-01 00:00:00pages
77issue
2eissn
2190-572Xissn
2190-5738pii
2052journal_volume
10pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0610-3
更新日期：2017-05-01 00:00:00
abstract：:We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0365-2
更新日期：2016-06-01 00:00:00
abstract：:Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved wit...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1315-y
更新日期：2018-07-01 00:00:00
abstract：:In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02397-w
更新日期：2020-09-01 00:00:00
abstract：:Human leukocyte antigen (HLA)-F has been involved in immune regulation of infectious diseases. However, the role of HLA-F polymorphisms in hepatitis B infection outcomes remains unclear. Here, we aimed to determine HLA-F polymorphism implication in chronic HBV. Genotype analysis was performed for three single nucleoti...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1079-9
更新日期：2018-01-01 00:00:00
abstract：:Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0788-4
更新日期：2017-07-01 00:00:00
abstract：:Electroporation parameters can be optimized by coupling RC constant values with the amount of electrical power dissipation in the electroporation medium. Electroporation efficiency increases more steeply with power at low power values. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0105-1
更新日期：2013-08-01 00:00:00
abstract：:The wound healing process is characterized by varied biological and molecular cascades including inflammation, tissue proliferation, and remodeling phase. To augment and maintain these cascades, an all-natural matrix system is proposed. Biocompatible biopolymers, sodium alginate and gelatin, were employed to prepare m...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02453-5
更新日期：2020-11-01 00:00:00
abstract：:The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K3Fe(CN)-6 as redo...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02315-0
更新日期：2020-07-01 00:00:00
abstract：:The molecular physiology of milk production of two important dairy species; Sahiwal cows (Bos indicus) and Murrah buffaloes (Bubalus bubalis) are not fully understood due to constraints in obtaining mammary tissue samples because of sacred and ethical reasons. The present study suggests the use of milk-derived mammary...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1642-7
更新日期：2019-03-01 00:00:00
abstract：:Parasporal crystalline inclusion proteins of some Bacillus spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of Bacillus sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystal...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-019-1849-7
更新日期：2019-09-01 00:00:00
abstract：:Today green synthesis of silver nanoparticles (SNPs) from plants is an utmost emerging filed in nanotechnology. In the present study, we have reported a green method for synthesis of SNPs from aqueous stem bark extract of Syzygium alternifolium, an endemic medicinal plant of South Eastern Ghats. These green-synthesise...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0307-4
更新日期：2015-12-01 00:00:00
abstract：:Cell-free extracts of twenty terrestrial cyanobacteria were evaluated for their antioxidant properties in terms of free-radical scavenging (DPPH and ABTS) and metal chelating activity and deoxyribose protection. Extract of Anabaena constricta was the most prominent antioxidant agent (IC50 for DPPH activity 0.91 mg ml-...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0786-6
更新日期：2017-06-01 00:00:00
abstract：:Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. T...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-016-0506-7
更新日期：2016-12-01 00:00:00
abstract：:The purpose of this study was to examine if fermentation of purple sweet potato (Ipomoea batatas L.) powder (PSP) by Lactobacillus brevis under green, red, blue, white light-emitting diode (LED) illumination or sunlight might yield functionalized products with good antibacterial, antioxidant activity, and/or cytotoxic...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1327-7
更新日期：2018-07-01 00:00:00
abstract：:Advancement in nanotechnology has improved ways for large-scale production and characterization of nanoparticles of physiologically important metals. The current study explores the impact of Zinc Oxide Nanoparticles (ZnO-NP) and Chitosan-Zinc oxide nano-bioformulation (CH-ZnO) in tissue culture raised callus of Nicoti...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02469-x
更新日期：2020-11-01 00:00:00
abstract：:Thermal stress is one of the challenges to crop plants that negatively impacts crop yield. To overcome this ever-growing problem, utilization of regulatory mechanisms, especially microRNAs (miRNAs), that provide efficient and precise regulation in a targeted manner have been found to play determining roles. Besides th...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-018-1521-7
更新日期：2018-12-01 00:00:00
abstract：:The Trichoderma harzianum l-methioninase was purified 7.15-fold with a recovery of 47.9% and the specific activity of 74.4 U/mg of protein. The purified enzyme has an apparent molecular mass of 48 kDa on SDS-PAGE and exhibited maximum activity at pH 8 and 35 °C. The enzyme was catalytically stable below 50 °C and at a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02494-w
更新日期：2020-11-01 00:00:00
abstract：:This bibliometric-based review analyses the trends in 1010 published articles (1990 to mid-2018) on the high-value application of Chlorella, and illustrates the evolution and latest tendencies at a global level by the number of publications and their distribution, issuing institutions and countries or regions, the sou...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2102-0
更新日期：2020-03-01 00:00:00
abstract：:Kalopanax septemlobus is a medicinal woody species of the family Araliaceae, and the pharmaceutical properties of saponins obtained from K. septemlobus suggest that K. septemlobus has the potential to be a crude drug and dietary health supplement. In this study, we established cell suspension culture of K. septemlobus...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1090-9
更新日期：2018-01-01 00:00:00
abstract：:In this study, a DNA-based nanosensor using specific NH2 labeled single standard probe was developed against stn gene of Salmonella enterica in milk samples. The single-stranded DNA probe was immobilized on carboxylated multiwalled carbon nanotube and gold nanoparticle (c-MWCNT/AuNP) electrode using 1-Ethyl-3-(3-dimet...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1957-4
更新日期：2019-11-01 00:00:00
abstract：:In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate fo...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1983-2
更新日期：2019-12-01 00:00:00
abstract：:The aim of this study was to explore the neuroprotective effect and the underlying mechanism of erythropoietin (EPO) on the cortical neuronal cells insulted with oxygen and glucose deprivation (OGD). Different concentrations of EPO were used to determine the anti-apoptosis effect of EPO. In addition, PI3K inhibitor LY...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1667-y
更新日期：2019-04-01 00:00:00
abstract：:An efficient regeneration system via a combined pathway of organogenesis and somatic embryogenesis was developed for date palm (Phoenix dactylifera L.) cv. Mejhoul. Adventitious buds were obtained from shoot-tip explants with a frequency of 53.3% after 9 months of culture: 6 months on half-strength Murashige and Skoog...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1235-x
更新日期：2018-04-01 00:00:00
abstract：:An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incub...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2097-6
更新日期：2020-03-01 00:00:00
abstract：:In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. R...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1477-7
更新日期：2018-11-01 00:00:00
abstract：:The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was inves...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0795-5
更新日期：2017-07-01 00:00:00
abstract：:Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1213-3
更新日期：2018-04-01 00:00:00
abstract：:A novel polypyrrole nanowires coated by graphene oxide (PPy-NWs/GO) has been successfully synthesized by one-step electrochemical method, whose structure was different from previously reported PPy/GO composites. The microbial fuel cell equipped with PPy-NWs/GO as anode was fabricated and compared with PPy-NWs ones. Th...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1321-0
更新日期：2018-08-01 00:00:00
abstract：:The immobilization of enzymes improves their stability in non-conventional media such as organic solvents. In this work, the effects of solvents (DMSO, methanol, ethanol, and n-propanol) on the endoglucanase Cel9A activity and stability were studied. Then, the enzymes were stabilized by its immobilization on chitosan ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1794-5
更新日期：2019-07-01 00:00:00
