Role of Cys73 in the thermostability of farnesyl diphosphate synthase from Geobacillus stearothermophilus.

abstract：:Phylogenetic relationship of 22 FLPs was revealed on the basis of polymorphism in three genes namely 16S rDNA, Pseudomonas-specific and rpoD gene regions. The primers for 16S rDNA, Pseudomonas-specific region and rpoD gene region were amplifying a region of 1492, 990 and 760 bp, respectively, from all the isolates inv...

journal_title：3 Biotech

authors： Rajwar A,Sahgal M

更新日期：2016-06-01 00:00:00

abstract：:The immobilization of enzymes improves their stability in non-conventional media such as organic solvents. In this work, the effects of solvents (DMSO, methanol, ethanol, and n-propanol) on the endoglucanase Cel9A activity and stability were studied. Then, the enzymes were stabilized by its immobilization on chitosan ...

journal_title：3 Biotech

authors： Mohammadi M,Najavand S,Pazhang M

更新日期：2019-07-01 00:00:00

abstract：:Biomass and lipid yield under nutrient depleted and supplemented conditions of N and Mg in two freshwater isolates, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 from Meghalaya were investigated for biodiesel production. Both the strains, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 are oleaginous in nature h...

journal_title：3 Biotech

authors： Mandal MK,Chaurasia N

更新日期：2018-05-01 00:00:00

abstract：:Cytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~ 21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. To dissect the function of miRNAs in soybean CMS, a total...

journal_title：3 Biotech

authors： Ding X,Zhang H,Ruan H,Li Y,Chen L,Wang T,Jin L,Li X,Yang S,Gai J

更新日期：2019-01-01 00:00:00

abstract：:Second-generation bioethanol can be produced from various lignocellulosic biomasses such as wood, agricultural or forest residues. Lignocellulosic biomass is inexpensive, renewable and abundant source for bioethanol production. The conversion of lignocellulosic biomass to bioethanol could be a promising technology tho...

journal_title：3 Biotech

authors： Maurya DP,Singla A,Negi S

更新日期：2015-10-01 00:00:00

abstract：:2,4-dinitrotoluene (2,4-DNT) is a priority environmental xenobiotic pollutant which has toxic, mutagenic, and carcinogenic properties. Thus, its biodegradation by applying recent approaches such as taking advantage of plant-bacteria interactions is crucial. In this work, the genes from Burkholderia sp. R34, necessary ...

journal_title：3 Biotech

authors： Akkaya Ö,Arslan E

更新日期：2019-11-01 00:00:00

abstract：:Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and ...

journal_title：3 Biotech

authors： Salar RK,Purewal SS,Sandhu KS

更新日期：2017-07-01 00:00:00

abstract：:Hydrogen production by biological route is a potentially sustainable alternative. Nowadays, energy production from sustainable sources has become urgent for several countries as well as for international policies. In this perspective, hydrogen has gained substantial global attention as clean, sustainable, and versatil...

journal_title：3 Biotech

authors： Silva FTM,Bessa LP,Vieira LM,Moreira FS,de Souza Ferreira J,Batista FRX,Cardoso VL

更新日期：2019-04-01 00:00:00

abstract：:The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may a...

journal_title：3 Biotech

authors： Sharma C,Gulati S,Thakur N,Singh BP,Gupta S,Kaur S,Mishra SK,Puniya AK,Gill JPS,Panwar H

更新日期：2017-05-01 00:00:00

abstract：:Antibiotic resistance is a global public health issue and it is even more daunting in developing countries. The main objective of present study was to investigate molecular responses of antibiotic-resistant bacteria. The 48 bacterial strains, which were previously isolated and identified were subjected to disc diffusi...

journal_title：3 Biotech

authors： Saima S,Fiaz M,Manzoor M,Zafar R,Ahmed I,Nawaz U,Arshad M

更新日期：2020-09-01 00:00:00

abstract：:Although endophytic bacteria are known to colonize Panax ginseng, little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total...

journal_title：3 Biotech

authors： Hong CE,Kim JU,Lee JW,Lee SW,Jo IH

更新日期：2018-09-01 00:00:00

abstract：:Thermal stress is one of the challenges to crop plants that negatively impacts crop yield. To overcome this ever-growing problem, utilization of regulatory mechanisms, especially microRNAs (miRNAs), that provide efficient and precise regulation in a targeted manner have been found to play determining roles. Besides th...

journal_title：3 Biotech

authors： Gahlaut V,Baranwal VK,Khurana P

更新日期：2018-12-01 00:00:00

abstract：:The possibility of using Aspergillus terreus protease in detergent formulations was investigated. Sodium dodecyl sulfate (SDS) and native polyacrylamide gel electrophoresis indicated that the purified alkaline protease (148.9 U/mg) is a monomeric enzyme with a molecular mass of 16 ± 1 kDa. This was confirmed by liquid...

journal_title：3 Biotech

authors： Niyonzima FN,More SS

更新日期：2015-02-01 00:00:00

abstract：:Paddy soils represent the largest anthropogenic wetlands on earth. Soil drying and rewetting that occurs annually inflict significant stress on soil microbial activities in paddy soils. An incubation experiment of 60 years of paddy soil was conducted to simulate the conditions of paddy fields (25 °C and 75% air humidi...

journal_title：3 Biotech

authors： Bagheri-Novair S,Mirseyed Hosseini H,Etesami H,Razavipour T,Asgari Lajayer B,Astatkie T

更新日期：2020-11-01 00:00:00

abstract：:Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...

journal_title：3 Biotech

authors： Qu C,Zhang S,Zhao H,Chen J,Zuo Z,Sun X,Cheng Y,Xu Z,Liu G

更新日期：2020-10-01 00:00:00

abstract：:In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate fo...

journal_title：3 Biotech

authors： Mahatmanto T,Azizah I,Buchberger A,Stephanopoulos N

更新日期：2019-12-01 00:00:00

abstract：:In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the ...

journal_title：3 Biotech

authors： Zhang J,Zhang J,Cheng S,Yang W,Li S

更新日期：2019-11-01 00:00:00

abstract：:The complex starter culture for artificial pit mud (APMSC) hosts a wide variety of microbial communities that play a crucial role in Chinese strong-flavor Baijiu production. Based on its organoleptic properties, the quality of APMSC can be divided into normal and inferior quality grades. However, the relationship betw...

journal_title：3 Biotech

authors： Liu MK,Tang YM,Zhao K,Liu Y,Guo XJ,Tian XH,Ren DQ,Yao WC

更新日期：2019-03-01 00:00:00

abstract：:Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and c...

journal_title：3 Biotech

authors： Vishwanathan AS,Aiyer KS,Chunduri LA,Venkataramaniah K,Siva Sankara Sai S,Rao G

更新日期：2016-12-01 00:00:00

abstract：:Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron ...

journal_title：3 Biotech

authors： Jhansi K,Jayarambabu N,Reddy KP,Reddy NM,Suvarna RP,Rao KV,Kumar VR,Rajendar V

更新日期：2017-08-01 00:00:00

abstract：:Tomato is the world's second most cultivated vegetable. During cultivation or post-harvest storage, it is susceptible to more than 200 diseases caused by an array of pathogenic fungi, nematodes, bacteria, and viruses. Although wide range of chemical pesticides are currently available to manage plant diseases, continuo...

journal_title：3 Biotech

authors： Singh VK,Singh AK,Kumar A

更新日期：2017-08-01 00:00:00

abstract：:RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predict...

journal_title：3 Biotech

authors： Tang D,Wei F,Kashif MH,Munsif F,Zhou R

更新日期：2019-10-01 00:00:00

abstract：:Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to sig...

journal_title：3 Biotech

authors： Choudhary SB,Kumar M,Chowdhury I,Singh RK,Pandey SP,Sharma HK,Karmakar PG

更新日期：2016-06-01 00:00:00

abstract：:Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved wit...

journal_title：3 Biotech

authors： Yardimci GO,Cekmecelioglu D

更新日期：2018-07-01 00:00:00

abstract：:Our earlier studies have indicated that GA3, being a growth hormone, increases internodal length, in turn increasing sink strength and improving sucrose accumulation in sugarcane. In this study, transcriptomic level analysis was carried out on internodal samples of a high sugar accumulating variety (CoLk 94184) of sug...

journal_title：3 Biotech

authors： Chandra A,Roopendra K,Verma I

更新日期：2019-10-01 00:00:00

abstract：:In the current study, the production of novel glutaminase free l-asparaginase from a new microbial source (Pseudomonas resinovorans IGS-131) is reported. Optimization of l-asparaginase production using conventional and statistical optimization techniques resulted in an enzyme yield of 37.63 IU/mL, which was 3.45-fold ...

journal_title：3 Biotech

authors： Mihooliya KN,Nandal J,Kumari A,Nanda S,Verma H,Sahoo DK

更新日期：2020-04-01 00:00:00

abstract：:In this study, we determined using NIRS the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a CNDH population derived from a cross between Cheongcheong and Nagdong rice varieties. The results revealed a higher heritability percentage for the amylose content and comp...

journal_title：3 Biotech

authors： Bruno E,Choi YS,Chung IK,Kim KM

更新日期：2017-05-01 00:00:00

abstract：:We investigated the expression of anthocyanin structural genes and transcription factors (TFs) associated with varying anthocyanin content during different developmental stages (S1-S4) of the gerbera cultivars 'Nathasha' and 'Rosalin'. Accumulation of anthocyanin started at S1 and reached a maximum at S3 in both culti...

journal_title：3 Biotech

authors： Naing AH,Park DY,Park KI,Kim CK

更新日期：2018-09-01 00:00:00

abstract：:In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. R...

journal_title：3 Biotech

authors： Panichikkal AF,Prakasan P,Kizhakkepowathial Nair U,Kulangara Valappil M

更新日期：2018-11-01 00:00:00

abstract：:To decipher an evolutionary lineage between two different but important bacterial groups, i.e., Pseudomonas strain (γ-Proteobacteria) and Frankia strain (actinobacteria) growing in the same ecological niche in and around of an actinorhizal plant Hippophae salicifolia D. Don, genetic diversity and comparative molecular...

journal_title：3 Biotech

authors： Srivastava M,Kaushik MS,Srivastava A,Singh A,Verma E,Mishra AK

更新日期：2016-12-01 00:00:00