当前位置： SCI文献检索 > 3 Biotech期刊下所有文献 > An efficient and cost effective method of RNA extraction from mucilage, phenol and secondary metabolite rich bark tissue of tossa jute (C. olitorius L.) actively developing phloem fiber.

An efficient and cost effective method of RNA extraction from mucilage, phenol and secondary metabolite rich bark tissue of tossa jute (C. olitorius L.) actively developing phloem fiber.

Abstract：

:Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to significantly high lignin content. Therefore, understanding genetic factors underlying lignin biosynthesis in tossa jute holds promise for jute based product diversification. The major limiting factor in undertaking such study is unavailability of efficient protocol for RNA extraction at secondary growth active stage of tossa jute. Here we report a simplified, swift and cost effective protocol for isolating fairly good quality RNA from bark tissue of 65-days-old field grown tossa jute plant with active secondary growth. The purity, concentration and integrity of extracted RNA ascertained. To confirm downstream amenability, isolated RNA samples were reverse transcribed and PCR analysis done by using CCoAMT1 primer. Results established that method of RNA extraction presented here is an improvement over the other methods, particularly for bark tissue of field grown tossa jute at advance developmental stage. Therefore, present study will enhance our ability to understand expression pattern of fiber formation and maturation related genes in mature bark tissue that holds key for much talked genetic manipulation of fiber quality via lignin optimisation in the crop.

journal_name

3 Biotech

journal_title

3 Biotech

authors

Choudhary SB,Kumar M,Chowdhury I,Singh RK,Pandey SP,Sharma HK,Karmakar PG

doi

10.1007/s13205-016-0415-9

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

100

issue

1

eissn

2190-572X

issn

2190-5738

pii

10.1007/s13205-016-0415-9

journal_volume

6

pub_type

杂志文章

相关文献

3 Biotech文献大全
  • Biofilm formation in marine bacteria and biocidal sensitivity: interplay between a potent antibiofilm compound (AS162) and quorum-sensing autoinducers.

    abstract：:The capacity of two homoserine lactones to stimulate the marine bacteria Pseudoalteromonas ulvae (TC14 strain) for its capacity to form a biofilm when exposed to a potent antibiofilm compound AS162 is reported. Effective concentrations (EC50) of AS162 at 24 h, 48 h, and 72 h were, respectively, of 4.3, 4.4, and 6.0 µM...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1866-6

    authors： Gozoua E,Koffi-Nevry R,Blache Y

    更新日期：2019-09-01 00:00:00

  • Studies on efficient production of a novel l-asparaginase by a newly isolated Pseudomonas resinovorans IGS-131 and its heterologous expression in Escherichia coli.

    abstract：:In the current study, the production of novel glutaminase free l-asparaginase from a new microbial source (Pseudomonas resinovorans IGS-131) is reported. Optimization of l-asparaginase production using conventional and statistical optimization techniques resulted in an enzyme yield of 37.63 IU/mL, which was 3.45-fold ...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-020-2135-4

    authors： Mihooliya KN,Nandal J,Kumari A,Nanda S,Verma H,Sahoo DK

    更新日期：2020-04-01 00:00:00

  • A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation.

    abstract：:The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-st...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-014-0213-1

    authors： Prajapati VS,Trivedi UB,Patel KC

    更新日期：2015-04-01 00:00:00

  • Complete genome sequence of Bacillus velezensis LPL-K103, an antifungal cyclic lipopeptide bacillomycin L producer from the surface of lemon.

    abstract：:Bacillus velezensis LPL-K103, which shows strong antifungal function, was isolated from the surface of lemon in Beijing, China. The complete genome of B. velezensis LPL-K103 contains a circular chromosome of 3,933,292 bp (46.61% G+C content). According to genomic analysis, 4080 protein-coding genes, 113 RNAs (27 rRNAs...

    journal_title：3 Biotech

    pub_type：

    doi：10.1007/s13205-019-1995-y

    authors： Zhang Y,Wang Y,Qin Y,Li P

    更新日期：2020-01-01 00:00:00

  • Identification and analysis of RNA editing sites in chloroplast transcripts of kenaf (Hibiscus cannabinus L.).

    abstract：:RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins and genetic information. To reveal the composition and characteristic of RNA editing of kenaf chloroplast genome, the RNA editing sites in kenaf chloroplast were predict...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1893-3

    authors： Tang D,Wei F,Kashif MH,Munsif F,Zhou R

    更新日期：2019-10-01 00:00:00

  • Diversity and bioactive potentials of culturable heterotrophic bacteria from the surficial sediments of the Arabian Sea.

    abstract：:Marine sediments accommodate plethora of diverse microorganisms with varying ecological functions. In the present study, we isolated bacteria from surficial sediments of south east Arabian Sea (AS) and evaluated their bioactive potentials. A total of 131 isolates belonging to the phylum: γ-Proteobacteria (63%), Bacill...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-016-0556-x

    authors： Anas A,Nilayangod C,Jasmin C,Vinothkumar S,Parameswaran PS,Nair S

    更新日期：2016-12-01 00:00:00

  • Exploration of miRNA-mediated fertility regulation network of cytoplasmic male sterility during flower bud development in soybean.

    abstract：:Cytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~ 21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. To dissect the function of miRNAs in soybean CMS, a total...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-018-1543-1

    authors： Ding X,Zhang H,Ruan H,Li Y,Chen L,Wang T,Jin L,Li X,Yang S,Gai J

    更新日期：2019-01-01 00:00:00

  • Molecular characterization and an 80-bp indel polymorphism within the prolactin receptor (PRLR) gene and its associations with chicken growth and carcass traits.

    abstract：:The prolactin receptor (PRLR), a type I cytokine receptor, must bind prolactin (PRL) to act on target cells to mediate various physiological functions, including reproduction and lactation. This study identified an 80-bp insertion/deletion (indel) polymorphism in the 3'-untranslated region (3'-UTR) of the chicken PRLR...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1827-0

    authors： Liang K,Wang X,Tian X,Geng R,Li W,Jing Z,Han R,Tian Y,Liu X,Kang X,Li Z

    更新日期：2019-08-01 00:00:00

  • ACC deaminase-producing rhizosphere competent Bacillus spp. mitigate salt stress and promote Zea mays growth by modulating ethylene metabolism.

    abstract：:Plant growth-promoting rhizobacteria (PGPR) are known for growth promotion and mitigating environmental stresses. Here, we examined the propitiousness of three indigenous salt-tolerant PGPR, i.e., Bacillus subtilis (NBRI 28B), B. subtilis (NBRI 33 N), and B. safensis (NBRI 12 M) for plant growth promotion and salt str...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-020-2104-y

    authors： Misra S,Chauhan PS

    更新日期：2020-03-01 00:00:00

  • Bioactive characteristics of a semi-hard non-starter culture cheese made from raw or pasteurized sheep's milk.

    abstract：:In this study, the effect of pasteurization and use of starter cultures on physicochemical, microbiological and functional properties of a traditional Iranian semi-hard cheese (Lighvan cheese) was evaluated during stages of ripening (1, 60, 120 days). Profiles of polar metabolites were analyzed by gas-chromatography m...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-020-2075-z

    authors： Saidi V,Sheikh-Zeinoddin M,Kobarfard F,Soleimanian-Zad S

    更新日期：2020-03-01 00:00:00

  • Tobacco habituated and non-habituated subjects exhibit different mutational spectrums in head and neck squamous cell carcinoma.

    abstract：:Head and neck squamous cell carcinoma (HNSCC) is the sixth most common non-skin cancer in the world. Tobacco chewing is implicated with most of the cases of HNSCC but this type of cancer is increasing in non-tobacco chewers as well. This study was instigated to provide comprehensive variant and gene-level data in HNSC...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-014-0267-0

    authors： Rawal RM,Joshi MN,Bhargava P,Shaikh I,Pandit AS,Patel RP,Patel S,Kothari K,Shah M,Saxena A,Bagatharia SB

    更新日期：2015-10-01 00:00:00

  • Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings.

    abstract：:Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of coppe...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-017-0929-9

    authors： Nair PMG,Chung IM

    更新日期：2017-10-01 00:00:00

  • Nanotechnology based therapeutic application in cancer diagnosis and therapy.

    abstract：:Due to the lack of early diagnosis, cancer remains as one of the leading cause of human mortality. Inability to translate research into clinical trials and also inability of chemotherapeutics delivery to targeted tumor sites are major drawbacks in cancer therapeutics. With the emergence of nanomedicine, several nanopr...

    journal_title：3 Biotech

    pub_type： 杂志文章,评审

    doi：10.1007/s13205-019-1940-0

    authors： Singh R

    更新日期：2019-11-01 00:00:00

  • Molecular mechanism, diagnosis, and potential treatment for novel coronavirus (COVID-19): a current literature review and perspective.

    abstract：:Novel coronavirus disease 2019 (COVID-19) is a positive-sense single-stranded RNA virus which belongs to the Coronaviridae family. COVID-19 outbreak became evident after the severe acute respiratory syndrome coronavirus and the Middle East respiratory syndrome coronavirus in the twenty-first century as the start of th...

    journal_title：3 Biotech

    pub_type： 杂志文章,评审

    doi：10.1007/s13205-021-02657-3

    authors： Ratre YK,Kahar N,Bhaskar LVKS,Bhattacharya A,Verma HK

    更新日期：2021-02-01 00:00:00

  • Comparative study on the antileishmanial activities of chemically and biologically synthesized silver nanoparticles (AgNPs).

    abstract：:The present study was conducted to investigate the antileishmanial activity of biogenic silver nanoparticles (AgNPs) compared to chemically synthesized AgNPs. A nano dimension size (10-15 nm) biogenic AgNPs was produced and characterized by UV-Vis spectroscopy and X-rays diffraction. The chemically synthesized AgNPs w...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-018-1121-6

    authors： Ullah I,Cosar G,Abamor ES,Bagirova M,Shinwari ZK,Allahverdiyev AM

    更新日期：2018-02-01 00:00:00

  • Emerging roles of mistletoes in malignancy management.

    abstract：:Mistletoes are a group of obligate plant semi-parasites in the order Santalales. These clumps of plants growing on a wide range of host plants have been traditionally regarded as medicinal repositories. However, current scientific discoveries have validated their health potentials like never before. Their extracts con...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-013-0124-6

    authors： Patel S,Panda S

    更新日期：2014-02-01 00:00:00

  • Genome-wide characterization of the UDP-glycosyltransferase gene family in upland cotton.

    abstract：:Uridine diphosphate (UDP)-glycosyltransferases (UGTs) involved in many metabolic processes are ubiquitous in plants, animals, microorganisms and other organisms and are essential for their growth and development. Upland cotton contains a large number of UGT genes. In this study, we aimed to identify UGT family members...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1984-1

    authors： Xiao X,Lu Q,Liu R,Gong J,Gong W,Liu A,Ge Q,Li J,Shang H,Li P,Deng X,Li S,Zhang Q,Niu D,Chen Q,Shi Y,Zhang H,Yuan Y

    更新日期：2019-12-01 00:00:00

  • Isolation and characterization of bacterial endophytes of Curcuma longa L.

    abstract：:Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-016-0393-y

    authors： Kumar A,Singh R,Yadav A,Giri DD,Singh PK,Pandey KD

    更新日期：2016-06-01 00:00:00

  • Diversity of bacterial endophytes in Panax ginseng and their protective effects against pathogens.

    abstract：:Although endophytic bacteria are known to colonize Panax ginseng, little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total...

    journal_title：3 Biotech

    pub_type：

    doi：10.1007/s13205-018-1417-6

    authors： Hong CE,Kim JU,Lee JW,Lee SW,Jo IH

    更新日期：2018-09-01 00:00:00

  • Saccharification of water hyacinth biomass by a combination of steam explosion with enzymatic technologies for bioethanol production.

    abstract：:In the present work, bioethanol was produced by sugar fermentation obtained from water hyacinth using a novelty hybrid method composed of steam explosion and enzymatic hydrolysis, using hydrolytic enzymes produced by solid-state fermentation and water hyacinth as substrate. The highest activity, 42 U for xylanase and ...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-020-02426-8

    authors： Figueroa-Torres LA,Lizardi-Jiménez MA,López-Ramírez N,Varela-Santos EC,Hernández-Rosas F,Favela-Torres E,Hernández-Martínez R

    更新日期：2020-10-01 00:00:00

  • Encapsulation of epigallocatechin-3-gallate into albumin nanoparticles improves pharmacokinetic and bioavailability in rat model.

    abstract：:In the present study, we fabricated epigallocatechin-3-gallate (EGCG) loaded albumin nanoparticles (Alb-NP-EGCG) to enhance bioavailability and improve pharmacokinetic parameters of EGCG. The physicochemical properties of the Alb-NP-EGCG were studied using scanning electron microscopy, differential scanning calorimetr...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1772-y

    authors： Ramesh N,Mandal AKA

    更新日期：2019-06-01 00:00:00

  • Construction of a synthetic Araneus ventricosus dragline silk gene multimer and its expression in Escherichia coli.

    abstract：:One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was clo...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-018-1285-0

    authors： Liu T,Liang A,Liang Z,Li G,Wang F

    更新日期：2018-05-01 00:00:00

  • Transgenic tomatoes for abiotic stress tolerance: status and way ahead.

    abstract：:Tomato (Solanum lycopersicum) is one of the most important vegetable crops; its production, productivity and quality are adversely affected by abiotic stresses. Abiotic stresses such as drought, extreme temperature and high salinity affect almost every stage of tomato life cycle. Depending upon the plant stage and dur...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1665-0

    authors： Krishna R,Karkute SG,Ansari WA,Jaiswal DK,Verma JP,Singh M

    更新日期：2019-04-01 00:00:00

  • Synthesis, characterization and antimicrobial properties of green-synthesised silver nanoparticles from stem bark extract of Syzygium alternifolium (Wt.) Walp.

    abstract：:Today green synthesis of silver nanoparticles (SNPs) from plants is an utmost emerging filed in nanotechnology. In the present study, we have reported a green method for synthesis of SNPs from aqueous stem bark extract of Syzygium alternifolium, an endemic medicinal plant of South Eastern Ghats. These green-synthesise...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-015-0307-4

    authors： Yugandhar P,Haribabu R,Savithramma N

    更新日期：2015-12-01 00:00:00

  • Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi.

    abstract：:Statistical methodologies, including Plackett-Burman design and Box-Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for max...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-017-1015-z

    authors： Guo L,Wang C

    更新日期：2017-12-01 00:00:00

  • Isolation and in silico characterization of novel esterase gene with β-lactamase fold isolated from metagenome of north western Himalayas.

    abstract：:An esterase-producing clone Aph2 was isolated from the Apharwat soil metagenomic library, a mountain peak in NW Himalayas. ORF 2 (Est Ac) of clone Aph2 corresponds to 271 aa protein and showed 26 % sequence similarity to carboxylesterase gene of Synechococcus sp. JA-2-3B. Est Ac contains nucleophilic Ser in S68-X-X-K7...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-014-0254-5

    authors： Sudan AK,Vakhlu J

    更新日期：2015-08-01 00:00:00

  • An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol.

    abstract：:Second-generation bioethanol can be produced from various lignocellulosic biomasses such as wood, agricultural or forest residues. Lignocellulosic biomass is inexpensive, renewable and abundant source for bioethanol production. The conversion of lignocellulosic biomass to bioethanol could be a promising technology tho...

    journal_title：3 Biotech

    pub_type： 杂志文章,评审

    doi：10.1007/s13205-015-0279-4

    authors： Maurya DP,Singla A,Negi S

    更新日期：2015-10-01 00:00:00

  • Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies.

    abstract：:The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of digestives enzymes; amylase, protease and lipase on to Zn-ferrite (ZnFe2O4). The metal ferrite was synthesized via a sol-gel technique and characterized with scanning ...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-017-0859-6

    authors： Adeogun AI,Kareeem SO,Adebayo OS,Balogun SA

    更新日期：2017-07-01 00:00:00

  • Progress toward sourcing plants for new bioconjugation tools: a screening evaluation of a model peptide ligase using a synthetic precursor.

    abstract：:In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate fo...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-019-1983-2

    authors： Mahatmanto T,Azizah I,Buchberger A,Stephanopoulos N

    更新日期：2019-12-01 00:00:00

  • Pectin from muskmelon (Cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties.

    abstract：:Pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. Optimization of pectin extraction from muskmelon peel by response surface methodology (RSM) was investigated in this study. Box-Behnken Design (BBD) was used to identify the optimal level of the extraction variable...

    journal_title：3 Biotech

    pub_type： 杂志文章

    doi：10.1007/s13205-017-0655-3

    authors： Muthukumaran C,Banupriya L,Harinee S,Sivaranjani S,Sharmila G,Rajasekar V,Kumar NM

    更新日期：2017-05-01 00:00:00