Abstract:
:Within a decade, MERS-CoV emerged with nearly four times higher case fatality rate than an earlier outbreak of SARS-CoV and spread out in 27 countries in short span of time. As an emerging virus, combating it requires an in-depth understanding of its molecular machinery. Therefore, conformational characterization studies of coronavirus proteins are necessary to advance our knowledge of the matter for the development of antiviral therapies. In this study, MERS-CoV papain-like protease (PLpro) was recombinantly expressed and purified. Thermal folding pathway and thermodynamic properties were characterized using dynamic multimode spectroscopy (DMS) and thermal shift assay. DMS study showed that the PLpro undergoes a single thermal transition and follows a pathway of two-state folding with T m and van't Hoff enthalpy values of 54.4 ± 0.1 °C and 317.1 ± 3.9 kJ/mol, respectively. An orthogonal technique based on intrinsic tryptophan fluorescence also showed that MERS-CoV PLpro undergoes a single thermal transition and unfolds via a pathway of two-state folding with a T m value of 51.4 °C. Our findings provide significant understandings of the thermodynamic and structural properties of MERS-CoV PLpro.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Malik A,Alsenaidy MAdoi
10.1007/s13205-017-0744-3subject
Has Abstractpub_date
2017-06-01 00:00:00pages
100issue
2eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-017-0744-3journal_volume
7pub_type
杂志文章相关文献
3 Biotech文献大全abstract::In this study, toxicity of biologically synthesized silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) was compared using zebrafish as a model organism. At 96 h, LC50 of AgNPs and AuNPs was found to be 24.5 µg/L and 41 mg/L, respectively. Following the LC50 determination, half of the LC50 of AgNPs (12.25 µg/L...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1457-y
更新日期:2018-10-01 00:00:00
abstract::Today green synthesis of silver nanoparticles (SNPs) from plants is an utmost emerging filed in nanotechnology. In the present study, we have reported a green method for synthesis of SNPs from aqueous stem bark extract of Syzygium alternifolium, an endemic medicinal plant of South Eastern Ghats. These green-synthesise...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-015-0307-4
更新日期:2015-12-01 00:00:00
abstract::Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of Tribulus terrestris was screened upon chromoge...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1695-7
更新日期:2019-04-01 00:00:00
abstract::Our earlier studies have indicated that GA3, being a growth hormone, increases internodal length, in turn increasing sink strength and improving sucrose accumulation in sugarcane. In this study, transcriptomic level analysis was carried out on internodal samples of a high sugar accumulating variety (CoLk 94184) of sug...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1908-0
更新日期:2019-10-01 00:00:00
abstract::Superoxide dismutases (SODs) play important roles in plant growth, development, and response to abiotic stresses. Despite SOD gene families have been identified in various plant species, little is known in foxtail millet (Setaria italica L.). In this study, a systematic analysis of SOD gene family was performed in fox...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1502-x
更新日期:2018-12-01 00:00:00
abstract::Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoi...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0729-2
更新日期:2017-06-01 00:00:00
abstract::Second-generation bioethanol can be produced from various lignocellulosic biomasses such as wood, agricultural or forest residues. Lignocellulosic biomass is inexpensive, renewable and abundant source for bioethanol production. The conversion of lignocellulosic biomass to bioethanol could be a promising technology tho...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-015-0279-4
更新日期:2015-10-01 00:00:00
abstract::Tossa jute is an important natural fiber crop of Southeast Asian countries including India, Bangladesh, China, Thailand, Myanmar etc. Traditional industrial application of jute fiber is limited to the packaging products like hessians, sacks, etc. and the fiber found unsuitable for textile industries largely due to sig...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0415-9
更新日期:2016-06-01 00:00:00
abstract::Long-term production in commercial straw biogas plants has been rare in China due to inefficiencies in the logistics stream. Biomass densification could be a potential solution to this issue. Therefore, we conducted a study to evaluate whether biomass densification is a more efficient and sustainable option. We perfor...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0914-3
更新日期:2017-10-01 00:00:00
abstract::This study was conducted to examine the role of the transcription factors (TFs), RsMYB1 and mPAP1 together with B-Peru (mPAP1 + B-Peru), in regulating anthocyanin biosynthesis in the ornamental torenia (Torenia fournieri) cultivar Kauai Rose using Agrobacterium-mediated transformation. Expression levels of RsMYB1 were...
journal_title:3 Biotech
pub_type:
doi:10.1007/s13205-018-1505-7
更新日期:2018-11-01 00:00:00
abstract::Screening for diabetic kidney disease (DKD) remains a challenge; however, there has been an ongoing research to investigate the diagnostic value of different biomarkers to identify DKD. The aim of this study was to assess the diagnostic value of both N-acetyl-beta-d-glucosaminidase (NAG) and neutrophil gelatinase-asso...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1593-z
更新日期:2019-03-01 00:00:00
abstract::The current study aimed at exploring the diversity of bacterial lactase genes in the intestinal mucosa of mice with dysbacterial diarrhea induced by antibiotics and to provide experimental basis for antibiotics-induced diarrhea. Mice model of dysbacterial diarrhea was established by gastric perfusion with mixture of c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1191-5
更新日期:2018-03-01 00:00:00
abstract::The purpose of this study was to determine the effect of ionized calcium on bacteria cross contamination on chicken carcass and meat during the slaughter process. Compared to the control group, colony of E. coli was not observed on medium containing 0.5% ionized calcium. Cross contamination of bacteria on carcass surf...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1489-3
更新日期:2018-11-01 00:00:00
abstract::This investigation demonstrates an efficient method of propagation, short-term conservation, and germplasm exchange for Plectranthus amboinicus (Lour.) Spreng. encapsulated propagules. In vitro-derived shoot apices (shoot tips and nodal segments) which showed 100% survival on MS medium supplemented with 0.4 mg/L 6-ben...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1831-4
更新日期:2019-08-01 00:00:00
abstract::In the present study, we have experimentally and theoretically studied the free-radical quenching property of dihydrocanaric acid (DCA) isolated from seedpods of Holarrhena antidysenterica. A modified method was used to estimate the nitric oxide scavenging effect of the DCA (significant activity of 75.22%) along with ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02221-5
更新日期:2020-06-01 00:00:00
abstract::Xanthine oxidase is a key enzyme responsible for hyperuricemia, a pre-disposing factor for Gout and oxidative stress-related diseases. Only two clinically approved xanthine oxidase inhibitors Allopurinol and Febuxostat are currently used for treatment of hyperuricemia. However, owing to their side effects there is a n...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0569-5
更新日期:2016-12-01 00:00:00
abstract::Eight exopolysaccharide (EPS) producing metal-removing marine bacteria were screened for mercury (Hg) sorption. Bacillus licheniformis with the highest MIC values and Hg sorption ability was selected for further study. Biosorption of Hg from aqueous solution by Bacillus licheniformis was studied with respect to the me...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0958-4
更新日期:2017-10-01 00:00:00
abstract::High-throughput small RNA sequencing and degradome analysis were used in this study to thoroughly investigate the role of miRNA-mediated regulatory network in tuberous root development of radish. Samples from the early seedling stage (RE) and the cortex splitting stage (RL) were used for the construction of six small ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1330-z
更新日期:2018-07-01 00:00:00
abstract::We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0365-2
更新日期:2016-06-01 00:00:00
abstract::Genome sequence of Paenibacillus polymyxa ND25 isolated from cow rumen is reported for being a potential candidate in hydrolysis of lignocellulosic plant biomass. Draft genome sequence generated 5.73 Mb data containing 4922 putative protein coding genes, of which 140 are annotated for glycoside hydrolases. P. polymyxa...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1274-3
更新日期:2018-05-01 00:00:00
abstract::Parthenium hysterophorus is a noxious weed in America, Asia, Africa and Australia. This weed is considered to be a cause of allergic respiratory problems, contact dermatitis, mutagenicity in human and livestock. Crop production is drastically reduced owing to its allelopathy. Also aggressive dominance of this weed thr...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-011-0007-7
更新日期:2011-07-01 00:00:00
abstract::This study was aimed at investigating the phytochemical constituents, antifungal properties and antibiotic-modifying activity of the aqueous crude extract and fractions of Amburana cearensis seeds (CEFAC). The CEFAC were chemically characterized by LC-MS/MS-QTOF. In addition, the antifungal activity was assayed by the...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02551-4
更新日期:2020-12-01 00:00:00
abstract::Several proteins and peptides of microbial origin are reported for their elicitor properties, which play a vital role in the development of local and systemic resistances in plants. In this study, the efficacy of total crude proteins (TCP) extracted from six different Trichoderma spp. (T. asperellum, T. harzianum, T. ...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-017-0816-4
更新日期:2017-07-01 00:00:00
abstract::Today, the role of nanotechnology in human life is undeniable as a broad range of industries, particularly food and medicine sectors, have been dramatically influenced. Nanomaterials can contribute to food safety by forming new nano-sized ingredients with modified physicochemical characteristics. Nanotechnologies can ...
journal_title:3 Biotech
pub_type: 杂志文章,评审
doi:10.1007/s13205-020-02409-9
更新日期:2020-10-01 00:00:00
abstract::Low conversion efficiency and long-processing time are some of the major problems associated with the use of biocatalysts in industrial processes. In this study, modified magnetic iron oxide nanoparticles bearing tyrosinase (tyrosinase-MNPs) were employed as a magnetic nano-biocatalyst to treat phenol-containing waste...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-018-1445-2
更新日期:2018-10-01 00:00:00
abstract::One of the most important rapidly emerging mosquito-borne alphavirus is Chikungunya virus (CHIKV). There is a necessity to develop anti-CHIKV therapeutics, as neither antiviral drug nor vaccines have been licensed yet. Several CHIKV proteins are being studied worldwide, but non-structural protein 3 (nsP3) has been les...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1759-8
更新日期:2019-06-01 00:00:00
abstract::Adenosine triphosphate (ATP) is produced at the early stage of seed germination and provides the energy for metabolism. The source of ATP in seeds may be Perl's pathway, but this has not yet been confirmed. In this study, using germinating seeds of poplar as the experimental materials, the transcript levels of genes r...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02413-z
更新日期:2020-10-01 00:00:00
abstract::Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and c...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-016-0552-1
更新日期:2016-12-01 00:00:00
abstract::The Trichoderma harzianum l-methioninase was purified 7.15-fold with a recovery of 47.9% and the specific activity of 74.4 U/mg of protein. The purified enzyme has an apparent molecular mass of 48 kDa on SDS-PAGE and exhibited maximum activity at pH 8 and 35 °C. The enzyme was catalytically stable below 50 °C and at a...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-020-02494-w
更新日期:2020-11-01 00:00:00
abstract::Uridine diphosphate (UDP)-glycosyltransferases (UGTs) involved in many metabolic processes are ubiquitous in plants, animals, microorganisms and other organisms and are essential for their growth and development. Upland cotton contains a large number of UGT genes. In this study, we aimed to identify UGT family members...
journal_title:3 Biotech
pub_type: 杂志文章
doi:10.1007/s13205-019-1984-1
更新日期:2019-12-01 00:00:00