Encapsulation of in vitro Plectranthus amboinicus (Lour.) Spreng. shoot apices for propagation and conservation.

Abstract:

:This investigation demonstrates an efficient method of propagation, short-term conservation, and germplasm exchange for Plectranthus amboinicus (Lour.) Spreng. encapsulated propagules. In vitro-derived shoot apices (shoot tips and nodal segments) which showed 100% survival on MS medium supplemented with 0.4 mg/L 6-benzylaminopurine were selected for encapsulation studies. Shoot apices measuring about 3-5 mm in size showed the ability to break the beads and exhibited 100% survival and regrowth. The combination of 3% (w/v) sodium alginate and 100 mM CaCl2 was found to be ideal for forming uniformally spherical beads, and successive preservation of encapsulated shoot apices into plantlets. The encapsulated shoot tips were relatively more effective than the nodal segments in terms of shoot growth and multiplication. Encapsulated shoot tips retained the ability to regrow (63.3%) for up to 40 days when maintained at 4 °C. Encapsulated shoot tips effectively converted into plantlets on agar medium (78%) and peat moss (58%) under in vitro conditions. Encapsulated shoot tips on agar medium showed a higher shoot regeneration (9.91 ± 0.15 shoots per explant) ability than the peat moss (5.71 ± 0.34 shoots per explant), while the highest rooting (12.16 ± 0.23 roots per explant) was observed on peat moss. Thus, calcium alginate encapsulation holds latent qualities that could be explored to develop a future alternative method of propagation, short-term storage and germplasm distribution for elite genotypes of Plectranthus sp.

journal_name

3 Biotech

journal_title

3 Biotech

authors

Arumugam G,Sinniah UR,Swamy MK,Lynch PT

doi

10.1007/s13205-019-1831-4

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

298

issue

8

eissn

2190-572X

issn

2190-5738

pii

1831

journal_volume

9

pub_type

杂志文章
  • Antinociceptive and anti-inflammatory activity of α-d-mannan from Pseudozyma sp.

    abstract::Pseudozyma sp. are yeasts that are commercially important due to their production of glycolipid biosurfactants, squalene, itaconic acid, and exopolysaccharide. The search for other analgesia inducing drugs, such as opiates and non-steroidal anti-inflammatory drugs (NSAIDs), as alternatives is beneficial. In this study...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-020-02635-1

    authors: Valasques Junior GL,Dos Santos JDG,Chaves PFP,Cordeiro LMC,de Jesus CL,de Lima FO,Boffo EF,de Assis SA

    更新日期:2021-02-01 00:00:00

  • Disease management of tomato through PGPB: current trends and future perspective.

    abstract::Tomato is the world's second most cultivated vegetable. During cultivation or post-harvest storage, it is susceptible to more than 200 diseases caused by an array of pathogenic fungi, nematodes, bacteria, and viruses. Although wide range of chemical pesticides are currently available to manage plant diseases, continuo...

    journal_title:3 Biotech

    pub_type: 杂志文章,评审

    doi:10.1007/s13205-017-0896-1

    authors: Singh VK,Singh AK,Kumar A

    更新日期:2017-08-01 00:00:00

  • miRNomes involved in imparting thermotolerance to crop plants.

    abstract::Thermal stress is one of the challenges to crop plants that negatively impacts crop yield. To overcome this ever-growing problem, utilization of regulatory mechanisms, especially microRNAs (miRNAs), that provide efficient and precise regulation in a targeted manner have been found to play determining roles. Besides th...

    journal_title:3 Biotech

    pub_type: 杂志文章,评审

    doi:10.1007/s13205-018-1521-7

    authors: Gahlaut V,Baranwal VK,Khurana P

    更新日期:2018-12-01 00:00:00

  • Combined effects of Allium sativum and Cuminum cyminum essential oils on planktonic and biofilm forms of Salmonella typhimurium isolates.

    abstract::Salmonella typhimurium (S. typhimurium) represents an important global public health problem and has the ability to survive under desiccation conditions in foods and food processing facilities for years. The aim of this study was to investigate the effects of Allium sativum (A. sativum) and Cuminum cyminum (C. cyminum...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-020-02286-2

    authors: Hakimi Alni R,Ghorban K,Dadmanesh M

    更新日期:2020-07-01 00:00:00

  • Improving agricultural straw preparation logistics stream in bio-methane production: experimental studies and application analysis.

    abstract::Long-term production in commercial straw biogas plants has been rare in China due to inefficiencies in the logistics stream. Biomass densification could be a potential solution to this issue. Therefore, we conducted a study to evaluate whether biomass densification is a more efficient and sustainable option. We perfor...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-017-0914-3

    authors: Tao L,Junting P,Xi M,Hailong H,Yan L,Xia X,Ruyi H,Zili M

    更新日期:2017-10-01 00:00:00

  • Recent biotechnological advances as potential intervention strategies against COVID-19.

    abstract::The emerging SARS-CoV-2 viral disease (COVID-19) has caused a global health alert due to its high rate of infection and mortality in individuals with chronic cardiovascular comorbidities, in addition to generating complex clinical conditions. This has forced the scientific community to explore different strategies tha...

    journal_title:3 Biotech

    pub_type: 杂志文章,评审

    doi:10.1007/s13205-020-02619-1

    authors: Lobo-Galo N,Gálvez-Ruíz JC,Balderrama-Carmona AP,Silva-Beltrán NP,Ruiz-Bustos E

    更新日期:2021-02-01 00:00:00

  • Fish trypsins: potential applications in biomedicine and prospects for production.

    abstract::In fishes, trypsins are adapted to different environmental conditions, and the biochemical and kinetic properties of a broad variety of native isoforms have been studied. Proteolytic enzymes remain in high demand in the detergent, food, and feed industries; however, our analysis of the literature showed that, in the l...

    journal_title:3 Biotech

    pub_type: 杂志文章,评审

    doi:10.1007/s13205-018-1208-0

    authors: Jesús-de la Cruz K,Álvarez-González CA,Peña E,Morales-Contreras JA,Ávila-Fernández Á

    更新日期:2018-04-01 00:00:00

  • MicroRNA-199a-5p suppresses the cell growth of colorectal cancer by targeting oncogene Caprin1.

    abstract::MicroRNAs-199a-5p (miR-199a-5p) plays critical regulatory roles in various types of human cancers. However, the biological function and regulatory mechanisms of miR-199a-5p in colorectal cancer (CRC) remain unclear. The aim of this study was to investigate the role of miR-199a-5p in CRC and possible mechanisms of its ...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-020-02433-9

    authors: Yu X,He Y,Wang Y,Tang Y,Guo Y,Yuan J,Bai J,Yao T,Wu X

    更新日期:2020-10-01 00:00:00

  • Chemical constituents, antioxidant and cytotoxicity properties of Leonotis leonurus used in the folklore management of neurological disorders in the Eastern Cape, South Africa.

    abstract::In the present study, we evaluated the phytochemical compounds and antioxidant properties of chloroform, ethanol and acetone extracts for leaves and flowers of Leonutusleonurus (L.leonurus) alongside with their cytotoxic effects on human cervical carcinoma (HeLa) cell lines. The phytochemical compounds present in the ...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-020-2126-5

    authors: Tonisi S,Okaiyeto K,Hoppe H,Mabinya LV,Nwodo UU,Okoh AI

    更新日期:2020-03-01 00:00:00

  • The influence of selenium on expression levels of the rbcL gene in Chlorella vulgaris.

    abstract::In this study, the effects of selenium on the microalgae Chlorella vulgaris were examined. Four groups of C. vulgaris were cultivated using Bristol medium: group I (control), no sodium selenite (Se); group II, 1 µM Se; group III, 10 µM Se; and group IV, 100 µM Se. Algal biomass samples were collected for biochemical e...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1212-4

    authors: Ozakman G,Yayman SG,Sezer Zhmurov C,Serdaroglu Kasikci E,Catal T

    更新日期:2018-04-01 00:00:00

  • Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean.

    abstract::The present work demonstrates the participation of polyamines (PAs) to improve direct regeneration and Agrobacterium-mediated transformation in soybean half-seeds. The inclusion of PAs to culture medium along with optimal plant growth regulators (PGRs) enhanced shoot induction [98.3 %; 4.44 µM N6-benzyladenine (BA) an...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-016-0448-0

    authors: Arun M,Chinnathambi A,Subramanyam K,Karthik S,Sivanandhan G,Theboral J,Alharbi SA,Kim CK,Ganapathi A

    更新日期:2016-12-01 00:00:00

  • A combined chloroplast atpB-rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India.

    abstract::Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by pl...

    journal_title:3 Biotech

    pub_type:

    doi:10.1007/s13205-016-0574-8

    authors: Shajitha PP,Dhanesh NR,Ebin PJ,Laly J,Aneesha D,Reshma J,Augustine J,Linu M

    更新日期:2016-12-01 00:00:00

  • Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01.

    abstract::Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-017-0755-0

    authors: Aggarwal NK,Goyal V,Saini A,Yadav A,Gupta R

    更新日期:2017-07-01 00:00:00

  • Enzymatic hydrolysis of tropical weed xylans using xylanase from Aureobasidium melanogenum PBUAP46 for xylooligosaccharide production.

    abstract::The maximum yield of xylanase from Aureobasidium melanogenum PBUAP46 was 5.19 ± 0.08 U ml-1 when cultured in a production medium containing 3.89% (w/v) rice straw and 0.75% (w/v) NaNO3 as carbon and nitrogen sources, respectively, for 72 h. This enzyme catalyzed well and was relatively stable at pH 7.0 and room temper...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-019-1586-y

    authors: Patipong T,Lotrakul P,Padungros P,Punnapayak H,Bankeeree W,Prasongsuk S

    更新日期:2019-02-01 00:00:00

  • Single and combined effects of acetic acid, furfural, and sugars on the growth of the pentose-fermenting yeast Meyerozyma guilliermondii.

    abstract::The tolerance of the pentose-fermenting yeast Meyerozyma guilliermondii to the inhibitors released after the biomass hydrolysis, such as acetic acid and furfural, was surveyed. We first verified the effects of acetic acid and cell concentrations and initial pH on the growth of a M. guilliermondii strain in a semi-synt...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1143-0

    authors: Perna MDSC,Bastos RG,Ceccato-Antonini SR

    更新日期:2018-02-01 00:00:00

  • Biocontrol potential of three novel Trichoderma strains: isolation, evaluation and formulation.

    abstract::We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-013-0150-4

    authors: Mukherjee AK,Sampath Kumar A,Kranthi S,Mukherjee PK

    更新日期:2014-06-01 00:00:00

  • High-throughput sequencing analysis of Eugenia uniflora: insights into repetitive DNA, gene content and potential biotechnological applications.

    abstract::In this study, the Ion Torrent PGM platform was employed to conduct a whole-genome sequencing analysis of Eugenia uniflora L., aiming to generate new genomic information for this non-model species. The whole-genome set of assembled sequences (WGSAS) generated 2601 contigs, with a cumulative length of 3.15 Mbp. About 2...

    journal_title:3 Biotech

    pub_type:

    doi:10.1007/s13205-019-1729-1

    authors: Stefenon VM,Sarzi DS,Roesch LFW

    更新日期:2019-05-01 00:00:00

  • Physiologic impact of 2-chlorophenol on denitrification process in mixture with different electron sources.

    abstract::The aim of this study was to evaluate the physiologic behavior of sludge in the absence and presence of 2-chlorophenol (2-CP) with different electron donors (phenol, glucose, and acetate) during denitrification process. In batch assays with phenol in the presence of 2-CP, a significant decrease of phenol consumption e...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-019-1723-7

    authors: Martínez-Gutiérrez E,Cuervo-López FM,Texier AC,Gómez J

    更新日期:2019-05-01 00:00:00

  • Role of phosphoglucomutase in regulating trehalose metabolism in Nilaparvata lugens.

    abstract::Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-020-2053-5

    authors: Pan BY,Liu YK,Wu HK,Pang XQ,Wang SG,Tang B,Xu CD

    更新日期:2020-02-01 00:00:00

  • Bioactive profile, free-radical scavenging potential, DNA damage protection activity, and mycochemicals in Aspergillus awamori (MTCC 548) extracts: a novel report on filamentous fungi.

    abstract::Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and ...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-017-0834-2

    authors: Salar RK,Purewal SS,Sandhu KS

    更新日期:2017-07-01 00:00:00

  • Medium composition potentially regulates the anthocyanin production from suspension culture of Daucus carota.

    abstract::In the present study, an effort has been made to optimize various culture conditions for enhanced production of anthocyanin. Nutrient content of MS medium (ammonium to potassium nitrate ratio and phosphate concentration) had a profound influence on the cell biomass and anthocyanin accumulation in cell suspension cultu...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1146-x

    authors: Saad KR,Parvatam G,Shetty NP

    更新日期:2018-03-01 00:00:00

  • Bacterial laccase: recent update on production, properties and industrial applications.

    abstract::Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are multi-copper enzymes which catalyze the oxidation of a wide range of phenolic and non-phenolic aromatic compounds in the presence or absence of a mediator. Till date, laccases have mostly been isolated from fungi and plants, whereas laccase from bacteria h...

    journal_title:3 Biotech

    pub_type: 杂志文章,评审

    doi:10.1007/s13205-017-0955-7

    authors: Chauhan PS,Goradia B,Saxena A

    更新日期:2017-10-01 00:00:00

  • A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation.

    abstract::The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-st...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-014-0213-1

    authors: Prajapati VS,Trivedi UB,Patel KC

    更新日期:2015-04-01 00:00:00

  • Molecular cloning and functional analysis of a Δ12-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.

    abstract::Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ12-fatty acid desaturase (FAD)-encoding gene (Δ 12 CiFAD), 1269 bp in size, was cloned from Ch...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-019-1858-6

    authors: He Y,Zheng Z,An M,Chen H,Qu C,Liu F,Wang Y,Miao J,Hou X

    更新日期:2019-09-01 00:00:00

  • Biotransformation of phenol in synthetic wastewater using the functionalized magnetic nano-biocatalyst particles carrying tyrosinase.

    abstract::Low conversion efficiency and long-processing time are some of the major problems associated with the use of biocatalysts in industrial processes. In this study, modified magnetic iron oxide nanoparticles bearing tyrosinase (tyrosinase-MNPs) were employed as a magnetic nano-biocatalyst to treat phenol-containing waste...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1445-2

    authors: Abdollahi K,Yazdani F,Panahi R,Mokhtarani B

    更新日期:2018-10-01 00:00:00

  • Genetic diversity and population structure of watermelon (Citrullus sp.) genotypes.

    abstract::Genetic polymorphism amid plant species is a crucial factor for plant improvement and maintaining their biodiversity. Evaluation of genetic diversity amongst plant species is significant to deal with the environmental stress conditions and their effective involvement in the breeding programs. Hence, in present study, ...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-019-1736-2

    authors: Pandey A,Khan MK,Isik R,Turkmen O,Acar R,Seymen M,Hakki EE

    更新日期:2019-06-01 00:00:00

  • Studies on growth dynamics of embryogenic cell suspension cultures of commercially important Indica rice cultivars ASD16 and Pusa basmati.

    abstract::Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1213-3

    authors: Sathish S,Venkatesh R,Safia N,Sathishkumar R

    更新日期:2018-04-01 00:00:00

  • Plant growth-promoting actinobacteria on chickpea seed mineral density: an upcoming complementary tool for sustainable biofortification strategy.

    abstract::The present study was evaluated to test the potential of plant growth-promoting actinobacteria in increasing seed mineral density of chickpea under field conditions. Among the 19 isolates of actinobacteria tested, significant (p < 0.05) increase of minerals over the uninoculated control treatments was noticed on all t...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-016-0458-y

    authors: Sathya A,Vijayabharathi R,Srinivas V,Gopalakrishnan S

    更新日期:2016-12-01 00:00:00

  • Exploration of miRNA-mediated fertility regulation network of cytoplasmic male sterility during flower bud development in soybean.

    abstract::Cytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~ 21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. To dissect the function of miRNAs in soybean CMS, a total...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1543-1

    authors: Ding X,Zhang H,Ruan H,Li Y,Chen L,Wang T,Jin L,Li X,Yang S,Gai J

    更新日期:2019-01-01 00:00:00

  • Heliomycin and tetracinomycin D: anthraquinone derivatives with histone deacetylase inhibitory activity from marine sponge-associated Streptomyces sp. SP9.

    abstract::Several actinomycetes strains were isolated from different marine sponges collected from the Red Sea shore in Egypt. The efficiency of their crude extracts to inhibit histone deacetylase (HDAC) enzyme was investigated in the nuclear extract of Hela cell line. The crude extract corresponding to Streptomyces sp. SP9 iso...

    journal_title:3 Biotech

    pub_type: 杂志文章

    doi:10.1007/s13205-018-1304-1

    authors: Abdelfattah MS,Elmallah MIY,Faraag AHI,Hebishy AMS,Ali NH

    更新日期:2018-06-01 00:00:00