Abstract：
:In the present study, an effort has been made to optimize various culture conditions for enhanced production of anthocyanin. Nutrient content of MS medium (ammonium to potassium nitrate ratio and phosphate concentration) had a profound influence on the cell biomass and anthocyanin accumulation in cell suspension cultures of Daucus carota. Suspension cultures were carried out in shake flasks for 18 days and examined for cell growth, anthocyanin synthesis, anthocyanin yield and development of pigmented cells in relation to the uptake of total sugar, extracellular phosphate, nitrate and ammonia. The addition of NH4NO3 to KNO3 ratio (20.0 mM: 37.6 mM) in the suspension culture media resulted in a 2.85-fold increase in anthocyanin content at day 3. Similarly, a lower concentration of KH2PO4 (0.45 mM) in the MS medium resulted in 1.63-fold increase in anthocyanin content at day 9. The total sugar uptake was closely associated with a significant increase in anthocyanin accumulation. Total sugar and nitrate were consumed until 9-12 days, while ammonia and phosphate were completely consumed within 3 days after inoculation. After 9 days, cell lysis was observed and resulted in the leakage of intracellular substances. These observations suggest that anthocyanin was synthesized only by viable pigmented cells and degraded rapidly after cell death and lysis. This study signifies the utility of D. carota suspension culture for further up-scaling studies of anthocyanin.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Saad KR,Parvatam G,Shetty NPdoi
10.1007/s13205-018-1146-xsubject
Has Abstractpub_date
2018-03-01 00:00:00pages
134issue
3eissn
2190-572Xissn
2190-5738pii
1146journal_volume
8pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5-1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0134-4
更新日期：2014-04-01 00:00:00
abstract：:One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was clo...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1285-0
更新日期：2018-05-01 00:00:00
abstract：:Plant growth-promoting fungi play an important role in development of sustainable agriculture. In the current study, 13 fungal strains were isolated from the rhizosphere of healthy Triticum aestivum (wheat) plant and screened for their indolic auxin production potential. Aspergillus flavus strain PGFW, Aspergillus nig...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1428-3
更新日期：2018-10-01 00:00:00
abstract：:Within a decade, MERS-CoV emerged with nearly four times higher case fatality rate than an earlier outbreak of SARS-CoV and spread out in 27 countries in short span of time. As an emerging virus, combating it requires an in-depth understanding of its molecular machinery. Therefore, conformational characterization stud...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0744-3
更新日期：2017-06-01 00:00:00
abstract：:The isolation of propiconazole (PCZ) degrading bacterium BBK_9 strain was done from paddy soil, and it was identified as Burkholderia sp. based on the morphological characteristics and biochemical properties combined with 16S rRNA gene sequencing analysis. It has been seen that the factors such as temperature and pH i...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0429-3
更新日期：2016-06-01 00:00:00
abstract：:After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0610-3
更新日期：2017-05-01 00:00:00
abstract：:Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of Tribulus terrestris was screened upon chromoge...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1695-7
更新日期：2019-04-01 00:00:00
abstract：:Biomass and lipid yield under nutrient depleted and supplemented conditions of N and Mg in two freshwater isolates, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 from Meghalaya were investigated for biodiesel production. Both the strains, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 are oleaginous in nature h...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1248-5
更新日期：2018-05-01 00:00:00
abstract：:This study assesses the potential for the lipid production by the oleaginous yeast Cystobasidium oligophagum JRC1 using dairy industry waste cheese whey as a substrate. Cheese whey was used either untreated (UCW) or deproteinized (DCW) at different concentrations (25-100%) to serve as the carbon and energy source. Bot...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1701-0
更新日期：2019-05-01 00:00:00
abstract：:In the present work, bioethanol was produced by sugar fermentation obtained from water hyacinth using a novelty hybrid method composed of steam explosion and enzymatic hydrolysis, using hydrolytic enzymes produced by solid-state fermentation and water hyacinth as substrate. The highest activity, 42 U for xylanase and ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02426-8
更新日期：2020-10-01 00:00:00
abstract：:Present study mainly aimed to ascertain the distribution characteristics of gene-based microsatellite loci and to develop polymorphic SSR markers from the already available transcriptome data of Elaeis guineensis Jacq, an important oil crop. From this study, we identified the sum of 5791 SSRs across 51,425 unigenes fr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02269-3
更新日期：2020-06-01 00:00:00
abstract：:Our earlier studies have indicated that GA3, being a growth hormone, increases internodal length, in turn increasing sink strength and improving sucrose accumulation in sugarcane. In this study, transcriptomic level analysis was carried out on internodal samples of a high sugar accumulating variety (CoLk 94184) of sug...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1908-0
更新日期：2019-10-01 00:00:00
abstract：:MicroRNAs-199a-5p (miR-199a-5p) plays critical regulatory roles in various types of human cancers. However, the biological function and regulatory mechanisms of miR-199a-5p in colorectal cancer (CRC) remain unclear. The aim of this study was to investigate the role of miR-199a-5p in CRC and possible mechanisms of its ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02433-9
更新日期：2020-10-01 00:00:00
abstract：:Actinomycetes strain was isolated from leaf litter soil sample and was identified as Streptomyces sp. by conventional and molecular approaches. The biologically active compound responsible for antimicrobial and anticancer activity of the strain JAR6 was elucidated by solid state fermentation followed by subsequent chr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1044-7
更新日期：2018-01-01 00:00:00
abstract：:Anthocyanins are major water-soluble and dynamic colouring plant pigment present in plant tissues with the high antioxidant properties. The role of ammonium and potassium nitrate in the culture medium on anthocyanin augmentation is probed thoroughly, but the mechanism of its biosynthesis continues to be unclear. Hence...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1447-0
更新日期：2018-10-01 00:00:00
abstract：:Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purificati...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0495-6
更新日期：2016-12-01 00:00:00
abstract：:Genetic polymorphism amid plant species is a crucial factor for plant improvement and maintaining their biodiversity. Evaluation of genetic diversity amongst plant species is significant to deal with the environmental stress conditions and their effective involvement in the breeding programs. Hence, in present study, ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1736-2
更新日期：2019-06-01 00:00:00
abstract：:Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation. Plasmid loss during bacterial cell divisions and structural changes in the plasmid DNA can dramatically reduce the...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0070-8
更新日期：2013-02-01 00:00:00
abstract：:Significant efforts are being directed towards the improvement of rice using genetic manipulations. A good and reproducible system for recovering fertile Indica rice plants is imperative. The aim of this study was to study the influence of initial cell density on growth dynamics of suspension cultures. The cultures we...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1213-3
更新日期：2018-04-01 00:00:00
abstract：:The complex nature of marine biodiversity is partially responsible for the lack of studies in Indian ascidian species, which often target a small number of novel biomolecules. We performed untargeted metabolomics using gas chromatography-mass spectrometry (GC-MS) in two invasive ascidian species to investigate the int...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1273-4
更新日期：2018-05-01 00:00:00
abstract：:The present study was designed to isolate an array of zinc solubilizing bacteria (ZSB) and to characterize them for plant growth promotion (PGP) attributes with respect to Capsicum annuum L. For this purpose, seventy bacteria were procured from cow dung and screened for zinc solubilization (ZnO and ZnCO3). Where, isol...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-2033-9
更新日期：2020-02-01 00:00:00
abstract：:In this study, we determined using NIRS the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a CNDH population derived from a cross between Cheongcheong and Nagdong rice varieties. The results revealed a higher heritability percentage for the amylose content and comp...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0687-8
更新日期：2017-05-01 00:00:00
abstract：:Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0109-x
更新日期：2013-12-01 00:00:00
abstract：:Escherichia coli is a faecal indicator and certain virotypes are known as pathogens. Therefore, detection and prevention of E. coli in food is very important. The existing rapid methods concentrate on detecting the pathogenic E. coli instead of total E. coli population. Present study evaluates the use of two molecular...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0784-8
更新日期：2017-06-01 00:00:00
abstract：:The emerging SARS-CoV-2 viral disease (COVID-19) has caused a global health alert due to its high rate of infection and mortality in individuals with chronic cardiovascular comorbidities, in addition to generating complex clinical conditions. This has forced the scientific community to explore different strategies tha...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-020-02619-1
更新日期：2021-02-01 00:00:00
abstract：:In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02397-w
更新日期：2020-09-01 00:00:00
abstract：:Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-020-2067-z
更新日期：2020-02-01 00:00:00
abstract：:Thermophilic bacterial communities generate thick biofilm on carbon steel API 5LX and produce extracellular metabolic products to accelerate the corrosion process in oil reservoirs. In the present study, nine thermophilic biocorrosive bacterial strains belonging to Bacillus and Geobacillus were isolated from the crude...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1604-0
更新日期：2019-03-01 00:00:00
abstract：:Cytoplasmic male sterility (CMS) plays an important role in the production of soybean hybrid seeds. MicroRNAs (miRNAs) are a class of non-coding endogenous ~ 21 nt small RNAs that play crucial roles in flower and pollen development by targeting genes in plants. To dissect the function of miRNAs in soybean CMS, a total...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1543-1
更新日期：2019-01-01 00:00:00
abstract：:Selection of the raw material and its efficient utilization are the critical factors in economization of second generation (2G) ethanol production. Fermentation of the released sugars into ethanol by a suitable ethanol producing microorganism using cheap media ingredients is the cornerstone of the overall process. Thi...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0098-9
更新日期：2013-10-01 00:00:00
