Complete genome and plasmid sequence of a novel Bacillus sp. BD59S, a parasporal protein synthesizing bacterium.

abstract：:Bacillus velezensis LPL-K103, which shows strong antifungal function, was isolated from the surface of lemon in Beijing, China. The complete genome of B. velezensis LPL-K103 contains a circular chromosome of 3,933,292 bp (46.61% G+C content). According to genomic analysis, 4080 protein-coding genes, 113 RNAs (27 rRNAs...

journal_title：3 Biotech

authors： Zhang Y,Wang Y,Qin Y,Li P

更新日期：2020-01-01 00:00:00

abstract：:A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5-1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In...

journal_title：3 Biotech

authors： Ashwini N,Srividya S

更新日期：2014-04-01 00:00:00

abstract：:In this study, we systemically investigated the positions and orientations of matrix attachment regions (MARs) in expression vectors to fully explore the mechanism for improving transgene expression. We constructed 14 vectors that incorporated human β-globin MARs into pIRES-eGFP backbone vectors. The MARs flanked the ...

journal_title：3 Biotech

authors： Zhang J,Zhang J,Cheng S,Yang W,Li S

更新日期：2019-11-01 00:00:00

abstract：:Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mellonella) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Strains were identified a...

journal_title：3 Biotech

authors： Moghaieb REA,Abdelhadi AA,El-Sadawy HA,Allam NAT,Baiome BA,Soliman MH

更新日期：2017-05-01 00:00:00

abstract：:G-box-binding factors are plant transcription factors, involved in a wide range of biological processes including abiotic stress responses. In this study, we analyzed the expression of OsGBF1 during salt stress in two contrasting Oryza sativa spp. indica genotypes, Rasi and Tellahamsa. Two-day-old seedlings were expos...

journal_title：3 Biotech

authors： Ashwini N,Sajeevan RS,Udayakumar M,Nataraja KN

更新日期：2018-08-01 00:00:00

abstract：:The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K3Fe(CN)-6 as redo...

journal_title：3 Biotech

authors： Saini K,Kaushal A,Gupta S,Kumar D

更新日期：2020-07-01 00:00:00

abstract：:Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1 and PGM2 were foun...

journal_title：3 Biotech

authors： Pan BY,Liu YK,Wu HK,Pang XQ,Wang SG,Tang B,Xu CD

更新日期：2020-02-01 00:00:00

abstract：:The present study was aimed to evaluate the immuno-modulatory effect of Brucella-specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice (n = 6 ...

journal_title：3 Biotech

authors： Minhas P,Sunil Kumar BV,Verma R

更新日期：2019-10-01 00:00:00

abstract：:Novel coronavirus disease 2019 (COVID-19) is a positive-sense single-stranded RNA virus which belongs to the Coronaviridae family. COVID-19 outbreak became evident after the severe acute respiratory syndrome coronavirus and the Middle East respiratory syndrome coronavirus in the twenty-first century as the start of th...

journal_title：3 Biotech

authors： Ratre YK,Kahar N,Bhaskar LVKS,Bhattacharya A,Verma HK

更新日期：2021-02-01 00:00:00

abstract：:A novel cadmium-resistant bacterium, Raoultella sp. strain X13, recently isolated from heavy metal-contaminated soil, and this strain can synthesize CdS quantum dots using cadmium nitrate [Cd(NO4)2] and l-cysteine. Biomineralization of CdS by strain X13 can efficiently remove cadmium from aqueous solution. To illumina...

journal_title：3 Biotech

authors： Xu S,Luo X,Xing Y,Liu S,Huang Q,Chen W

更新日期：2019-04-01 00:00:00

abstract：:Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV-Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs sho...

journal_title：3 Biotech

authors： Anbazhagan S,Azeez S,Morukattu G,Rajan R,Venkatesan K,Thangavelu KP

更新日期：2017-10-01 00:00:00

abstract：:One of the most representative core gene sequence of Araneus ventricosus dragline silk protein partial cDNA monomer (JN857964.2) was selected and multimerized using a "head-to-tail" strategy by compatible but nonregenerable sites at both ends resulting in a concatemer of 16 contiguous monomers. This concatemer was clo...

journal_title：3 Biotech

authors： Liu T,Liang A,Liang Z,Li G,Wang F

更新日期：2018-05-01 00:00:00

abstract：:The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After scr...

journal_title：3 Biotech

authors： Thorat AS,Sonone NA,Choudhari VV,Devarumath RM,Babu KH

更新日期：2017-05-01 00:00:00

abstract：:A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in E. coli was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by E. coli BL21 (pET22b-pelNK93I) was 10,181 U/mL which is the highest level so far. On this basis, to improve ...

journal_title：3 Biotech

authors： Zhen J,Tan M,Fu X,Shu W,Zhao X,Yang S,Xu J,Ma Y,Zheng H,Song H

更新日期：2020-02-01 00:00:00

abstract：:In this study, we determined using NIRS the heritability percentage of amylose, protein, and moisture content in polished and unpolished rice in a CNDH population derived from a cross between Cheongcheong and Nagdong rice varieties. The results revealed a higher heritability percentage for the amylose content and comp...

journal_title：3 Biotech

authors： Bruno E,Choi YS,Chung IK,Kim KM

更新日期：2017-05-01 00:00:00

abstract：:In fishes, trypsins are adapted to different environmental conditions, and the biochemical and kinetic properties of a broad variety of native isoforms have been studied. Proteolytic enzymes remain in high demand in the detergent, food, and feed industries; however, our analysis of the literature showed that, in the l...

journal_title：3 Biotech

authors： Jesús-de la Cruz K,Álvarez-González CA,Peña E,Morales-Contreras JA,Ávila-Fernández Á

更新日期：2018-04-01 00:00:00

abstract：:Biological methods of nanoparticles synthesis are ecologically sound and sustainable alternative to the conventional methods. On the basis of aforesaid premise, the present study deals with the optimization and fabrication of gold nanoparticles (Au-NPs) using easily available bio-resource, Delonix regia leaf extract. ...

journal_title：3 Biotech

authors： Dauthal P,Mukhopadhyay M

更新日期：2016-12-01 00:00:00

abstract：:Lysine-rich (Lys-rich) proteins encoded by AGP17, AGP18, and AGP19 genes are cell wall-associated glycopeptides related to sexual reproduction in flowering plants. This subclass belongs to classical arabinogalactan proteins (AGPs) widely studied in model plants like Arabidopsis. In this study, we identified the CaAGP1...

journal_title：3 Biotech

authors： Verdugo-Perales M,Velez-de la Rocha R,León-Félix J,Osuna-Enciso T,Heredia JB,Osuna-Castro JA,Islas-Osuna MA,Sañudo-Barajas JA

更新日期：2018-01-01 00:00:00

abstract：:Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in...

journal_title：3 Biotech

authors： Aggarwal NK,Goyal V,Saini A,Yadav A,Gupta R

更新日期：2017-07-01 00:00:00

abstract：:A detailed understanding of the fate of xenobiotics introduced into the environment and the mechanisms involved in their biotransformation, biodegradation, and biosorption is essential to improve the efficiency of remediation techniques. Mycoremediation is a form of bioremediation technique that has become increasingl...

journal_title：3 Biotech

authors： Kryczyk-Poprawa A,Piotrowska J,Żmudzki P,Opoka W,Muszyńska B

更新日期：2020-04-01 00:00:00

abstract：:In this study, the endophytic capacity of B. bassiana was determined for two barley (Hordeum vulgare) varieties, Josefa and Esmeralda, inoculated with a seedling immersion at three different concentrations (1 × 106, 1 × 107 and 1 × 108 conidia/mL). Seedling length and chlorophyll content were found to be not affected ...

journal_title：3 Biotech

authors： Veloz-Badillo GM,Riveros-Ramírez J,Angel-Cuapio A,Arce-Cervantes O,Flores-Chávez B,Espitia-López J,Loera O,Garza-López PM

更新日期：2019-01-01 00:00:00

abstract：:We sequenced the entire tb1 gene in six maize inbreds and its wild relatives (parviglumis, mexicana, perennis and luxurians) to characterize it at molecular level. Hopscotch and Tourist transposable elements were observed in the upstream of tb1 in all maize inbreds, while they were absent in wild relatives. In maize, ...

journal_title：3 Biotech

authors： Prakash NR,Chhabra R,Zunjare RU,Muthusamy V,Hossain F

更新日期：2020-02-01 00:00:00

abstract：:One of the most important rapidly emerging mosquito-borne alphavirus is Chikungunya virus (CHIKV). There is a necessity to develop anti-CHIKV therapeutics, as neither antiviral drug nor vaccines have been licensed yet. Several CHIKV proteins are being studied worldwide, but non-structural protein 3 (nsP3) has been les...

journal_title：3 Biotech

authors： Nandi I,Gupta A,Chaudhary VK,Gupta V,Gabrani R,Gupta S

更新日期：2019-06-01 00:00:00

abstract：:The wound healing process is characterized by varied biological and molecular cascades including inflammation, tissue proliferation, and remodeling phase. To augment and maintain these cascades, an all-natural matrix system is proposed. Biocompatible biopolymers, sodium alginate and gelatin, were employed to prepare m...

journal_title：3 Biotech

authors： Sharma A,Mittal A,Puri V,Kumar P,Singh I

更新日期：2020-11-01 00:00:00

abstract：:In this study, the effect of pasteurization and use of starter cultures on physicochemical, microbiological and functional properties of a traditional Iranian semi-hard cheese (Lighvan cheese) was evaluated during stages of ripening (1, 60, 120 days). Profiles of polar metabolites were analyzed by gas-chromatography m...

journal_title：3 Biotech

authors： Saidi V,Sheikh-Zeinoddin M,Kobarfard F,Soleimanian-Zad S

更新日期：2020-03-01 00:00:00

abstract：:Within a decade, MERS-CoV emerged with nearly four times higher case fatality rate than an earlier outbreak of SARS-CoV and spread out in 27 countries in short span of time. As an emerging virus, combating it requires an in-depth understanding of its molecular machinery. Therefore, conformational characterization stud...

journal_title：3 Biotech

authors： Malik A,Alsenaidy MA

更新日期：2017-06-01 00:00:00

abstract：:The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was inves...

journal_title：3 Biotech

authors： Ghimire BK,Son NY,Kim SH,Yu CY,Chung IM

更新日期：2017-07-01 00:00:00

abstract：:We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid...

journal_title：3 Biotech

authors： Shaikh SS,Wani SJ,Sayyed RZ

更新日期：2016-06-01 00:00:00

abstract：:Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of mult...

journal_title：3 Biotech

authors： Mittal J,Sharma MM

更新日期：2017-05-01 00:00:00

abstract：:Phylogenetic relationship of 22 FLPs was revealed on the basis of polymorphism in three genes namely 16S rDNA, Pseudomonas-specific and rpoD gene regions. The primers for 16S rDNA, Pseudomonas-specific region and rpoD gene region were amplifying a region of 1492, 990 and 760 bp, respectively, from all the isolates inv...

journal_title：3 Biotech

authors： Rajwar A,Sahgal M

更新日期：2016-06-01 00:00:00