Abstract：
:To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and their mRNA expression profiles were evaluated using quantitative real-time PCR. Flavour metabolite quantification by HPLC analysis confirmed 10, 170 and 500 µg/g 2-hydroxy-4-methoxy benzaldehyde and 4, 20 and 40 µg/g vanillin in first- (3-month-old plant), second- (18-month-old plant) and third-stage tubers (60-month-old matured plant), respectively. The expression of all four genes phenylalanine ammonia lyase (DhPAL), cinnamate-4-hydroxylase (DhC4H), caffeic acid-O-methyltransferase (DhCOMT) and vanillin synthase (DhVAN) increased with flavour development from first stage to second stage. A decrease in expression from 1.9-folds to 0.1-folds and 19.2-folds to 5.2-folds for DhCOMT and DhVAN was recorded for second stage to third stage, respectively. However, a gradual increase in expression of DhPAL (up to 26.4-folds) and a constant expression pattern for DhC4H (up to 7.1-folds) was evident from second stage to third stage of flavour development. The decrease in the expression levels of DhCOMT and DhVAN in third stage shows that the second-stage tubers are more transcriptionally active towards flavour biosynthesis.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Kiran K,Sonbarse PP,Veeresh L,Shetty NP,Parvatam Gdoi
10.1007/s13205-018-1388-7subject
Has Abstractpub_date
2018-08-01 00:00:00pages
365issue
8eissn
2190-572Xissn
2190-5738pii
1388journal_volume
8pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:The yeasts transmitted from seeds to sprouts might be used as probiotics for host plants. To investigate the inheritable yeasts of rice plants for probiotics, the fungal internal transcribed spacer (ITS) regions (ITS1 and ITS2) in rice sprouts were analyzed by Illumina-based sequencing. The fungal genera Candida, Mort...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1746-0
更新日期：2019-06-01 00:00:00
abstract：:Pythium aphanidermatum is one of the common causal pathogen of damping-off disease of beans (Phaseolus vulgaris L.) grown in Manipur. A total of 110 indigenous Trichoderma isolates obtained from North east India were screened for their biocontrol activity which can inhibit the mycelial growth of P. aphanidermatum, the...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0027-3
更新日期：2011-12-01 00:00:00
abstract：:Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by pl...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-016-0574-8
更新日期：2016-12-01 00:00:00
abstract：:Statistical experimental designs were used to optimize conditions for recombinant Burkholderia pyrrocinia feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal con...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02198-1
更新日期：2020-05-01 00:00:00
abstract：:Bacterial strains associated with entomopathogenic nematodes (EPNs) Rhabditis (Oscheius) spp. were isolated from infected cadavers of Galleria mellonella. The obtained 18 isolates were subdivided into nine phylogenetically different genera based on comparative sequence analysis of their 16S rRNA genes. The isolates we...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0326-1
更新日期：2016-06-01 00:00:00
abstract：:The action of FTO on myoblasts proliferation and differentiation and molecular mechanism underlying it were investigated by transfecting with FTO lentiviral overexpression vector and gene expression profile sequencing. Compared with the control group, myoblasts with FTO transfection was significantly enhanced prolifer...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02386-z
更新日期：2020-09-01 00:00:00
abstract：:The present study was aimed to evaluate the immuno-modulatory effect of Brucella-specific recombinant HSP40 (rDnaJ) when co-immunized with Brucella rOmp22 in mice. For this, dnaJ of Brucella abortus was cloned, expressed in E. coli, and purified to homogeneity using Ni-NTA agarose columns. Three groups of mice (n = 6 ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1905-3
更新日期：2019-10-01 00:00:00
abstract：:The emergence of new strains of Magnaporthe oryzae (M. oryzae) is associated with recurrent failure of resistance response mediated by single resistance (R) gene in rice. Therefore, stacking or combining of multiple R genes could improve the durability of resistance against multiple strains of M. oryzae. To achieve th...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1062-5
更新日期：2018-01-01 00:00:00
abstract：:Infectious diseases caused by antimicrobial-resistant microbes (ARMs) and the treatment are the serious problems in the field of medical science today world over. The development of alternative drug line to treat such infectious diseases is urgently required. Researches on ARMs revealed the presence of membrane protei...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0180-y
更新日期：2014-10-01 00:00:00
abstract：:Somatic embryogenesis is an important and wonderful biotechnological tool used to develop whole plant from a single or a group of somatic cells. The differentiated somatic cells become totipotent stem cells by drastic reprogramming of a wide range of cellular activities, leading to the acquisition of embryogenic compe...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-021-02649-3
更新日期：2021-02-01 00:00:00
abstract：:Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0393-y
更新日期：2016-06-01 00:00:00
abstract：:Only scanty reports are available on endophytic fungal associations in Achyranthes aspera Linn. Hence in this study a total of 504 isolates belonging to ten different species of fungi were isolated from asymptomatic, surface sterilised segments of leaf, stem and root of A. aspera collected from different locations of ...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-017-0748-z
更新日期：2017-06-01 00:00:00
abstract：:The molecular physiology of milk production of two important dairy species; Sahiwal cows (Bos indicus) and Murrah buffaloes (Bubalus bubalis) are not fully understood due to constraints in obtaining mammary tissue samples because of sacred and ethical reasons. The present study suggests the use of milk-derived mammary...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1642-7
更新日期：2019-03-01 00:00:00
abstract：:Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV-Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs sho...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0961-9
更新日期：2017-10-01 00:00:00
abstract：:Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast trans...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0315-4
更新日期：2016-06-01 00:00:00
abstract：:Emblica officinalis Gaertn. syn. Phyllanthus emblica L., universally known as 'Amla' or 'Aonla' or 'Indian gooseberry', is a popular fruit tree belonging to the family Euphorbiaceae and order Geraniales. It is said to be the very first tree that originated on earth, as claimed by age-old Indian mythology. Almost all p...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-020-02615-5
更新日期：2021-02-01 00:00:00
abstract：:This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechan...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02237-x
更新日期：2020-06-01 00:00:00
abstract：:Parthenium hysterophorus is a noxious weed in America, Asia, Africa and Australia. This weed is considered to be a cause of allergic respiratory problems, contact dermatitis, mutagenicity in human and livestock. Crop production is drastically reduced owing to its allelopathy. Also aggressive dominance of this weed thr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0007-7
更新日期：2011-07-01 00:00:00
abstract：:A 35 kDa rabbit erythrocyte agglutinating lectin from the seeds of Cicer arietinum was purified and designated as CAL. The lectin was inhibited by fetuin and N-acetyl-d-galactosamine at a concentration of 20 and 50 mM respectively, but not by simple mono or oligosaccharides. CAL is active between pH 5 and 10 presented...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1272-5
更新日期：2018-06-01 00:00:00
abstract：:In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic a...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02397-w
更新日期：2020-09-01 00:00:00
abstract：:Due to the lack of early diagnosis, cancer remains as one of the leading cause of human mortality. Inability to translate research into clinical trials and also inability of chemotherapeutics delivery to targeted tumor sites are major drawbacks in cancer therapeutics. With the emergence of nanomedicine, several nanopr...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-019-1940-0
更新日期：2019-11-01 00:00:00
abstract：:Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation. Plasmid loss during bacterial cell divisions and structural changes in the plasmid DNA can dramatically reduce the...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0070-8
更新日期：2013-02-01 00:00:00
abstract：:Nine (9) different date palm (Phoenix dactylifera L.) cultivars from UAE, which differ in their flower timings were selected to determine the polymorphism and genetic relationship between these cultivars. Hereditary differences and interrelationships were assessed utilizing inter-simple sequence repeat (ISSR) and dire...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1108-3
更新日期：2018-02-01 00:00:00
abstract：:Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0894-3
更新日期：2017-08-01 00:00:00
abstract：:Pseudozyma sp. are yeasts that are commercially important due to their production of glycolipid biosurfactants, squalene, itaconic acid, and exopolysaccharide. The search for other analgesia inducing drugs, such as opiates and non-steroidal anti-inflammatory drugs (NSAIDs), as alternatives is beneficial. In this study...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02635-1
更新日期：2021-02-01 00:00:00
abstract：:Although endophytic bacteria are known to colonize Panax ginseng, little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in P. ginseng plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-018-1417-6
更新日期：2018-09-01 00:00:00
abstract：:Achatina fulica (Lissachatina fulica) is one of the most invasive species found across the globe causing a significant damage to crops, vegetables, and horticultural plants. This terrestrial snail is native to east Africa and spread to different parts of the world by introductions. India, a hot spot for biodiversity o...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0877-4
更新日期：2017-08-01 00:00:00
abstract：:The effect of microwave (MW)-assisted acid or alkali pretreatment (300 W, 7 min) followed by saccharification with a triple enzyme cocktail (Cellic, Optimash BG and Stargen) with or without detoxification mix on ethanol production from three cassava residues (stems, leaves and peels) by Saccharomyces cerevisiae was in...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1095-4
更新日期：2018-01-01 00:00:00
abstract：:In the current study, aerial parts (leaves, stem and shoots) of C. album were extracted with methanol and subjected to phytochemical and HPLC analysis. Agar well diffusion method was used for anti-bacterial activity against Gram-negative strains Escherichia coli, Salmonella typhi, Klebsiella, Pseudomonas aeruginosa an...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02436-6
更新日期：2020-10-01 00:00:00
abstract：:The ability of gellan gum-immobilised cells of the heavy metal-tolerant bacterium Alcaligenes sp. AQ05-001 to utilise both heavy metal-free and heavy metal-polluted feathers (HMPFs) as substrates to produce keratinase enzyme was studied. Optimisation of the media pH, incubation temperature and immobilisation parameter...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1555-x
更新日期：2019-01-01 00:00:00
