Abstract:
:Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Seuring C,Ayyer K,Filippaki E,Barthelmess M,Longchamp JN,Ringler P,Pardini T,Wojtas DH,Coleman MA,Dörner K,Fuglerud S,Hammarin G,Habenstein B,Langkilde AE,Loquet A,Meents A,Riek R,Stahlberg H,Boutet S,Hunter MS,Kodoi
10.1038/s41467-018-04116-9subject
Has Abstractpub_date
2018-05-09 00:00:00pages
1836issue
1issn
2041-1723pii
10.1038/s41467-018-04116-9journal_volume
9pub_type
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