Abstract：
:The pathogenesis of acute myeloid leukaemias (AML) frequently requires at least 3 mutations in different cellular pathways. In many cases, mutations in proliferation/survival mechanisms and differentiation pathways are involved. Genetic aberrations explain the pathogenesis of AML, provide prognostic criteria and guide drug design for future therapy. Since the last update of the World Health Organization (WHO) classification of tumours of the hematopoietic and lymphoid tissues in 2008, there have been many discovers regarding the genomic landscape of AML and molecular assays for the detection of minimal residual disease. The identification of unique biomarkers associated with AML, derived from gene expression analysis and next-generation sequencing, may improve significantly the diagnostic criteria. In the new WHO classification published in 2017, several new AML entities are added. Moreover, the prognostic and diagnostic relevance of recently identified molecular features is reviewed and integrated into existing sets of criteria. This article reviews the most common tests and procedures for AML diagnosis, including morphology, immunophenotyping, cytogenetics and molecular genetic testing. The major changes in the new WHO classification for AML are also remarked.
journal_name
Int J Lab Hematoljournal_title
International journal of laboratory hematologyauthors
Merino A,Boldú L,Ermens Adoi
10.1111/ijlh.12831subject
Has Abstractpub_date
2018-05-01 00:00:00pages
109-119eissn
1751-5521issn
1751-553Xjournal_volume
40 Suppl 1pub_type
杂志文章abstract：INTRODUCTION:The investigation of platelet function by aggregometry requires specialist equipment and is labour intensive. We have developed an automated platelet aggregation method on a routine coagulation analyser. METHODS:We used a CS-2000i (Sysmex) with prototype software to perform aggregation in platelet-rich pl...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12161
更新日期：2014-08-01 00:00:00
abstract：INTRODUCTION:The study aims to evaluate the diagnostic utility of thyrogastric immune features in the identification of intrinsic factor antibody negative (IFA -ve) pernicious anaemia (PA) patients. METHODS:Clinico-pathological features of 'intrinsic factor antibody positive (IFA +ve) PA' and 'IFA -ve presumed PA' Chi...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2010.01295.x
更新日期：2011-08-01 00:00:00
abstract：INTRODUCTION:To retrospectively analyze epidemiological, clinical and hematological characteristics of COVID-19 patients. METHODS:The demographic, symptoms, and physiological parameters of 88 patients were collected and analyzed. The performance of complete blood count (CBC) indexes for monitoring and predicting the s...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13291
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:White blood cell (WBC) counts are used to monitor bone marrow function and to screen for infections. The HemoCue WBC DIFF Point-Of-Care (POC) instrument classifies WBCs through cell image recognition. To evaluate its suitability for monitoring cancer patients, we examined its performance in samples from pati...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13158
更新日期：2020-06-01 00:00:00
abstract：INTRODUCTION:Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is ...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12975
更新日期：2019-06-01 00:00:00
abstract：INTRODUCTION:The Hematoflow (Beckman Coulter, USA) is a new automated hematology analyzer, which provides a 16-part white blood cell count (WBC) differential. METHODS:We evaluated the differential WBC count performance of the Hematoflow. 101 blood samples from patients were selected for comparison analysis. RESULTS:T...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2012.01432.x
更新日期：2012-10-01 00:00:00
abstract：INTRODUCTION:In acute myeloid leukemia (AML), it has been shown that AML-derived cells often remain sensitive to autophagy-inducing stimuli, leading to the idea that harnessing the autophagy can be pertinent to AML cytotoxic therapy. Despite this promising notion, to date, there is no comprehensive study addressing aut...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12454
更新日期：2016-04-01 00:00:00
abstract：INTRODUCTION:In order to correctly manage the paediatric patients affected with haemostatic disorders, age-appropriate reference intervals should be used. The purpose of this study was to establish age-specific reference intervals for prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (aPT...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13098
更新日期：2019-10-01 00:00:00
abstract：:Cell counter-based formulae have been used in the differential diagnosis of microcytic anemia. The measurement of new red cell parameters is now available on the Sysmex XE 5000 analyzer. Derived from the percentages of microcytic and hypochromic red cells, the authors describe the new formula %microcytic-%hypochromic,...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2010.01237.x
更新日期：2011-02-01 00:00:00
abstract：INTRODUCTION:Venous blood (VB) sampling for complete blood count (CBC) via venipuncture is the basic method for the daily evaluation of hematological patients. However, several issues during this process, such as venipuncture difficulty and repetitive attempts, may cause pain, phlebitis, hematomas, inadequate sampling,...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13237
更新日期：2020-10-01 00:00:00
abstract：:The paucity of clinical reports in the world literature suggests that, as a disease entity, haemoglobin SE compound heterozygosity is of negligible importance. In view of the significant community prevalence of this haemoglobinopathy in the Sultanate of Oman where it is the second most prevalent sickling disorder, a h...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2257.2006.00886.x
更新日期：2007-08-01 00:00:00
abstract：INTRODUCTION:We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS:We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12774
更新日期：2018-06-01 00:00:00
abstract：:Advances in lab-on-a-chip technologies enabled programmable, reconfigurable, and scalable manipulation of a variety of laboratory procedures. Samples, reagents, and fluids can be precisely controlled; buffer temperature, pH, and concentration control systems as well as a variety of detection systems can be integrated ...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章,评审
doi：10.1111/ijlh.12054
更新日期：2013-10-01 00:00:00
abstract：INTRODUCTION:New generation osmotic gradient ektacytometry has become a powerful procedure for measuring red blood cell deformability and therefore for the diagnosis of red blood cell membrane disorders. In this study, we aim to provide further support to the usefulness of osmotic gradient ektacytometry for the differe...
journal_title：International journal of laboratory hematology
pub_type： 临床试验,杂志文章
doi：10.1111/ijlh.12746
更新日期：2018-02-01 00:00:00
abstract：INTRODUCTION:Lupus anticoagulant (LAC) testing is a multistep procedure including screening, mixing, and confirmation tests. STA Coag Expert is a software module for STA R Max and STA Compact Max analyzers which includes an on-demand LAC algorithm, based on ISTH guidelines, for automatic interpretation, calculation, an...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13001
更新日期：2019-06-01 00:00:00
abstract：:This 22-year-old women presented to the ante-natal clinic of this hospital for prenatal screening for beta-thalassemia. Cation exchange high performance liquid chromatography (HPLC) using 'Beta Thalassemia Short Program' on Bio-Rad 'Variant' system revealed HbC value of 81.6%. The CBC showed microcytic hypochromic ane...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1365-2257.2006.00850.x
更新日期：2007-10-01 00:00:00
abstract：INTRODUCTION:D-dimer (DD) assays are effective for the exclusion of deep-vein thrombosis (DVT), but point-of-care (POC) DD assays have not been fully evaluated. METHODS:We have compared five POC DD assays (Pathfast, Cardiac, Vidas, Stratus and NycoCard) with our routine DD method (Tinaquant), testing 60 samples from p...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2012.01421.x
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Lnc-IRF2-3 and Lnc-ZNF667-AS1 were recently studied as a positive biomarker for many tumor cells. However, experimental studies found that they are associated with worse outcomes in B-CLL. METHODS:A prospective case study was conducted on 135 B-CLL patients that were compared to thirty healthy controls. The...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13167
更新日期：2020-06-01 00:00:00
abstract：INTRODUCTION:Presence of schistocytes in peripheral blood smear supporting haemolysis is important for diagnosis and decision-making in paediatric haemolytic uraemic syndrome (HUS). High observer dependency and requirement of expertise for peripheral smear evaluation propels us to think of other modalities to overcome ...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13324
更新日期：2021-02-01 00:00:00
abstract：INTRODUCTION:The blood count provides qualitative and quantitative essential information on bloodlines. Reference hematologic parameters have been established in children and neonates, but few data are available regarding the premature population during the first month of life. The main objective of this study was to e...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12629
更新日期：2017-06-01 00:00:00
abstract：:In the 8 years since the publication of the 4th edition of the WHO Classification of Hematopoietic and Lymphoid Tumours in 2008, there has been rapid accumulation of knowledge in the molecular genetics, biology, clinical behavior of many hematologic malignancies. Concepts and models have advanced to the point at which...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章,评审
doi：10.1111/ijlh.12650
更新日期：2017-05-01 00:00:00
abstract：INTRODUCTION:FVIII inhibitors consist of a polyclonal population of antibodies. Previous studies have demonstrated different distribution of IgG subclasses. IgG4 was associated to high level of FVIII inhibitors and failure of immune tolerance induction (ITI) treatment. This study monitored the relative distribution of ...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12696
更新日期：2017-12-01 00:00:00
abstract：:The progressive shortening of telomeres and the activation of telomerase are considered to be one of the important mechanisms in cellular immortalization and disease progression. Bone marrow samples were collected from 148 patients with acute leukemia (AL). Based on the stage of the disease, patients were divided into...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2009.01178.x
更新日期：2010-04-01 00:00:00
abstract：INTRODUCTION:Thrombocytopenia-absent radius (TAR) syndrome is a rare autosomal recessive disease. Patients are compound heterozygotes for a loss-of-function allele, which in most cases is a large genomic deletion on chromosome 1q21.1 containing the RBM8A gene, and a noncoding variant located in the 5'UTR (rs139428292) ...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.12516
更新日期：2016-08-01 00:00:00
abstract：:Successful adoptive immunotherapy for leukemia depends on the generation of T cells that can specifically react with malignant cells. Dendritic cells (DCs) are important antigen-presenting cells in the development of antileukemia T-cell responses. Mononuclear cells (MNC) were isolated from peripheral blood or bone mar...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2007.00986.x
更新日期：2008-10-01 00:00:00
abstract：:The foundation of laboratory hematologic diagnosis is the complete blood count and review of the peripheral smear. In patients with anemia, the peripheral smear permits interpretation of diagnostically significant red blood cell (RBC) findings. These include assessment of RBC shape, size, color, inclusions, and arrang...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章,评审
doi：10.1111/ijlh.12082
更新日期：2013-06-01 00:00:00
abstract：:Blood coagulation factor XIII (FXIII) plays a key role in the protection of fibrin clot against fibrinolysis, in the cross-linking of fibrin and its mechanical strength. The role of the FXIII-A subunit Val34Leu polymorphism with fatal primary intracerebral hemorrhages (PICH) has not been studied. We evaluated retrospe...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2012.01465.x
更新日期：2013-02-01 00:00:00
abstract：:The PENTRA 60C(+) hematology analyzer provides a complete blood cell (CBC) count, including a five-part differential (5-DIFF) count and two leukocyte subpopulations, i.e. large immature cells (LIC's) and atypical lymphocytes (ALY's). We evaluated its analytical performance and assessed agreement with the ADVIA 2120, i...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/j.1751-553X.2007.01011.x
更新日期：2009-04-01 00:00:00
abstract：INTRODUCTION:Clinical reference intervals represent the normal range of clinical parameters for distinguishing healthy and sick individuals, and they show some variation among different populations. Many reference intervals are still lacking for the pediatric population in China. Thus, the aim of this study was to esta...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13150
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:Hematological patients, receiving intensive chemotherapy (predominantly acute leukemia patients), have repeated postchemotherapy periods with severe bone marrow suppression. As a result, these patients require regular monitoring of the complete blood counts (CBC) for optimal patient care. To reduce the strai...
journal_title：International journal of laboratory hematology
pub_type： 杂志文章
doi：10.1111/ijlh.13330
更新日期：2021-02-01 00:00:00
