Acute myeloid leukaemia: How to combine multiple tools.


:The pathogenesis of acute myeloid leukaemias (AML) frequently requires at least 3 mutations in different cellular pathways. In many cases, mutations in proliferation/survival mechanisms and differentiation pathways are involved. Genetic aberrations explain the pathogenesis of AML, provide prognostic criteria and guide drug design for future therapy. Since the last update of the World Health Organization (WHO) classification of tumours of the hematopoietic and lymphoid tissues in 2008, there have been many discovers regarding the genomic landscape of AML and molecular assays for the detection of minimal residual disease. The identification of unique biomarkers associated with AML, derived from gene expression analysis and next-generation sequencing, may improve significantly the diagnostic criteria. In the new WHO classification published in 2017, several new AML entities are added. Moreover, the prognostic and diagnostic relevance of recently identified molecular features is reviewed and integrated into existing sets of criteria. This article reviews the most common tests and procedures for AML diagnosis, including morphology, immunophenotyping, cytogenetics and molecular genetic testing. The major changes in the new WHO classification for AML are also remarked.


Int J Lab Hematol


Merino A,Boldú L,Ermens A




Has Abstract


2018-05-01 00:00:00








40 Suppl 1


  • Telomerase activity and telomere length in acute leukemia: correlations with disease progression, subtypes and overall survival.

    abstract::The progressive shortening of telomeres and the activation of telomerase are considered to be one of the important mechanisms in cellular immortalization and disease progression. Bone marrow samples were collected from 148 patients with acute leukemia (AL). Based on the stage of the disease, patients were divided into...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Wang Y,Fang M,Sun X,Sun J

    更新日期:2010-04-01 00:00:00

  • Hemostasis critical values among Iranian clinical laboratories "National Survey of 157 Clinical Laboratories".

    abstract:BACKGROUND:Immediate reporting of critical values or test results significantly outside the normal range has a growing role in the management of patients, especially in life-threatening conditions. Due to the lack of international consensus, diverse approaches are used for determination of thresholds, reporting, docume...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Dorgalaleh A,Shiravand Y,Dabbagh A,Tabibian S,Hosseini MS,Mansouri Tourghabeh H,Rashidpanah J,Narouei F,Shams M,Gholami MS,Saneei Moghaddam E,Shahreki H

    更新日期:2019-12-01 00:00:00

  • Osmotic gradient ektacytometry: A valuable screening test for hereditary spherocytosis and other red blood cell membrane disorders.

    abstract:INTRODUCTION:New generation osmotic gradient ektacytometry has become a powerful procedure for measuring red blood cell deformability and therefore for the diagnosis of red blood cell membrane disorders. In this study, we aim to provide further support to the usefulness of osmotic gradient ektacytometry for the differe...

    journal_title:International journal of laboratory hematology

    pub_type: 临床试验,杂志文章


    authors: Llaudet-Planas E,Vives-Corrons JL,Rizzuto V,Gómez-Ramírez P,Sevilla Navarro J,Coll Sibina MT,García-Bernal M,Ruiz Llobet A,Badell I,Velasco-Puyó P,Dapena JL,Mañú-Pereira MM

    更新日期:2018-02-01 00:00:00

  • Guidance for storing blood samples in laboratories performing complete blood count with differential.

    abstract:INTRODUCTION:The complete blood count (CBC) with differential leukocyte count (DIFF) is an important part of clinical laboratory analyses and provides crucial data for clinicians. Delivery time after blood collection and conditions of storage is known to affect the reliability of results of some hematologic parameters....

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Cornet E,Behier C,Troussard X

    更新日期:2012-12-01 00:00:00

  • Three novel alternative splicing mutations in BCR-ABL1 detected in CML patients with resistance to kinase inhibitors.

    abstract:INTRODUCTION:Multiple types of mutations in the BCR-ABL1 kinase domain have been reported. We previously reported a common alternatively spliced BCR-ABL mRNA with a 35-nucleotide insertion (35INS). We report three novel alternative splicing mutants expressed as the dominant transcripts in patient with chronic myelogeno...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Ma W,Giles F,Zhang X,Wang X,Zhang Z,Lee TS,Yeh CH,Albitar M

    更新日期:2011-06-01 00:00:00

  • 2016 WHO Classification update-What's new in lymphoid neoplasms.

    abstract::In the 8 years since the publication of the 4th edition of the WHO Classification of Hematopoietic and Lymphoid Tumours in 2008, there has been rapid accumulation of knowledge in the molecular genetics, biology, clinical behavior of many hematologic malignancies. Concepts and models have advanced to the point at which...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Hsi ED

    更新日期:2017-05-01 00:00:00

  • Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing.

    abstract:INTRODUCTION:Clinical reference intervals represent the normal range of clinical parameters for distinguishing healthy and sick individuals, and they show some variation among different populations. Many reference intervals are still lacking for the pediatric population in China. Thus, the aim of this study was to esta...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Sun Q,Yue Y,Tian Y,Wang Q

    更新日期:2020-10-01 00:00:00

  • The impact of long-haul air travel on variables of the athlete's biological passport.

    abstract:INTRODUCTION:Dehydration, fluid shifts or changes in coagulation occurring during air travel can trigger distinct reactions in the haematological system. Athletes are concerned that these effects might impair sporting performance, increase the risk of thrombosis or cause abnormalities in blood values that might be mist...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Schumacher YO,Klodt F,Nonis D,Pottgiesser T,Alsayrafi M,Bourdon PC,Voss SC

    更新日期:2012-12-01 00:00:00

  • Evaluating the analytical performance of five new coagulation assays for the measurement of prothrombin time and activated thromboplastin time.

    abstract:INTRODUCTION:New methods for coagulation tests require careful assessment before routine use. We evaluated the analytical performance of five new coagulation assays for measuring prothrombin time (PT) and activated partial thromboplastin time (aPTT). METHODS:PT Rec, PT Owren, aPTT, aPTT Lupus and aPTT Screen assays (R...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Kitchen S,Geisen U,Kappelmayer J,Quehenberger P,Drieß J,Lowe A,Jones R,Boehm JG,Miles G,Rozsnyai G

    更新日期:2018-12-01 00:00:00

  • Strategies for basic laboratory diagnostics of the hemoglobinopathies in multi-ethnic societies: interpretation of results and pitfalls.

    abstract::The consistent multi-ethnic migrations of the last decades have considerably changed the epidemiology of the hemoglobinopathies. Healthy carriers of these conditions are present today in many nonendemic parts of the world, and severely affected children are now born where these diseases were previously rare or unknown...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Giordano PC

    更新日期:2013-10-01 00:00:00

  • Establishment of reference intervals for complete blood count parameters in venous blood for children in the Xiamen area, China.

    abstract:OBJECTIVES:To observe changes in the complete blood count (CBC) venous blood parameters of healthy children and establish appropriate reference intervals (RIs). METHODS:Overall, 4066 healthy children were selected and divided into four groups: infants, toddlers, preschool-age children and school-age children. Venous b...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Li J,Zhang H,Huang X,Zhang J,Wu X

    更新日期:2019-10-01 00:00:00

  • Evaluation of the leukocyte differential on a new automated flow cytometry hematology analyzer.

    abstract:INTRODUCTION:The Hematoflow (Beckman Coulter, USA) is a new automated hematology analyzer, which provides a 16-part white blood cell count (WBC) differential. METHODS:We evaluated the differential WBC count performance of the Hematoflow. 101 blood samples from patients were selected for comparison analysis. RESULTS:T...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Kim JE,Kim BR,Woo KS,Han JY

    更新日期:2012-10-01 00:00:00

  • Development of a biochip-based assay integrated in a global strategy for identification of fusion transcripts in acute myeloid leukemia: a work flow for acute myeloid leukemia diagnosis.

    abstract::Three major types of rearrangements are involved in acute myeloid leukemias (AML): t(8;21)(q22;q22), inv(16)(p13q22), and 11q23/MLL abnormalities. Their precise identification becomes essential for diagnosis, prognosis, and therapeutic choices. Resulting fusion transcripts (FT) are also powerful markers for monitoring...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Giusiano S,Formisano-Tréziny C,Benziane A,Maroc N,Picard C,Hermitte F,Taranger-Charpin C,Gabert J

    更新日期:2010-08-01 00:00:00

  • The importance of β globin deletion analysis in the evaluation of patients with β thalassemia.

    abstract:INTRODUCTION:Beta globin deletion/duplication analysis may serve as a useful adjunct to sequence analysis. Our purpose was to develop a robust assay for beta globin deletion/duplication analysis and determine its role in evaluating patients with beta thalassemia. METHODS:A single tube semi-quantitative fluorescent PCR...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Mikula M,Buller-Burckle A,Gallivan M,Sun W,Franklin CR,Strom CM

    更新日期:2011-06-01 00:00:00

  • Detection of malignant cells in serous body fluids by counting high-fluorescent cells on the Sysmex XN-2000 hematology analyzer.

    abstract:INTRODUCTION:The body fluid mode of the Sysmex XN-2000 hematology analyzer differentiates cells into mononuclear and polymorphonuclear white blood cells (WBC) and high-fluorescent cells (HFC). The aim of this study was to evaluate the performance of the HFC count for detecting malignant cells in serous body fluids. ME...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Labaere D,Boeckx N,Geerts I,Moens M,Van den Driessche M

    更新日期:2015-10-01 00:00:00

  • Paroxysmal nocturnal haemoglobinuria. Experience over a 10 years period.

    abstract:INTRODUCTION:Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic, clonal and acquired disorder of the hematopoietic stem cell with a deficiency of all glycophosphatidyl-inositol (GPI) linked proteins. The aim of this retrospective study was to analyse haematological and biochemical data from 152 patients referred ...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Canalejo K,Riera Cervantes N,Felippo M,Sarandría C,Aixalá M

    更新日期:2014-04-01 00:00:00

  • Accuracy of serum transferrin receptor levels in the diagnosis of iron deficiency among hospital patients in a population with a high prevalence of thalassaemia trait.

    abstract::The diagnosis of iron deficiency in hospital patients can be difficult in the presence of inflammation. A raised serum transferrin receptor (sTfR) level is useful as a marker of iron deficiency as it is unaffected by inflammation. However, diseases that cause an increase in erythropoietic activity can also result in a...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Ong KH,Tan HL,Tam LP,Hawkins RC,Kuperan P

    更新日期:2008-12-01 00:00:00

  • Peripheral blood vs. bone marrow for molecular monitoring of BCR-ABL1 levels in chronic myelogenous leukemia, a retrospective analysis in allogeneic bone marrow recipients.

    abstract::Molecular monitoring of the BCR-ABL1 transcript in chronic myelogenous leukemia (CML) using quantitative real-time PCR (RQ-PCR) can be performed using either bone marrow (BM) or peripheral blood (PB). However, a recent report by Stock et al. [International Journal of Oncology 28 (2006) 1099] questioned the reliability...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Ballestrero A,Cirmena G,Dominietto A,Garuti A,Rocco I,Cea M,Moran E,Nencioni A,Miglino M,Raiola AM,Bacigalupo A,Patrone F

    更新日期:2010-08-01 00:00:00

  • Association of mean platelet volume and platelet count with the development and prognosis of ischemic and hemorrhagic stroke.

    abstract:INTRODUCTION:Mean platelet volume (MPV) and platelet (PLT) count are the two major parameters that reflect the functions and activities of PLTs. The associations of MPV and PLT count with the occurrence and prognosis of stroke have not been fully clarified. This study aimed to investigate the association of MPV and PLT...

    journal_title:International journal of laboratory hematology

    pub_type: 临床试验,杂志文章


    authors: Du J,Wang Q,He B,Liu P,Chen JY,Quan H,Ma X

    更新日期:2016-06-01 00:00:00

  • Flow cytometry in the diagnosis of mature B-cell lymphoproliferative disorders.

    abstract::B-lineage lymphoproliferative disorders (LPD) are rather frequent diseases, associated with specific clinical or biological features but also sometimes of fortuitous discovery. Multiparameter flow cytometry plays a major role for a rapid diagnostic indication, on peripheral blood or bone marrow samples in most instanc...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Debord C,Wuillème S,Eveillard M,Theisen O,Godon C,Le Bris Y,Béné MC

    更新日期:2020-06-01 00:00:00

  • How I investigate Eosinophilia.

    abstract::Eosinophilia is typically secondary, that is, reactive, in nature and is associated with a wide variety of neoplastic and non-neoplastic disorders. Clonal eosinophilia is also seen in a wide variety of hematopoietic neoplasms, and sub-classification can be diagnostically challenging. A proper evaluation of persistent ...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Larsen RL,Savage NM

    更新日期:2019-04-01 00:00:00

  • Molecular diagnosis of thrombocytopenia-absent radius syndrome using next-generation sequencing.

    abstract:INTRODUCTION:Thrombocytopenia-absent radius (TAR) syndrome is a rare autosomal recessive disease. Patients are compound heterozygotes for a loss-of-function allele, which in most cases is a large genomic deletion on chromosome 1q21.1 containing the RBM8A gene, and a noncoding variant located in the 5'UTR (rs139428292) ...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Nicchia E,Giordano P,Greco C,De Rocco D,Savoia A

    更新日期:2016-08-01 00:00:00

  • Abrogation of red blood cell G6PD enzyme activity through Heat treatment: development of survey material for the UK NEQAS G6PD scheme.

    abstract:INTRODUCTION:Participation in external quality assessment (EQA) is central to the maintenance of high-quality laboratory results in patient diagnosis and clinical trials. Laboratories in the TAF112582 DETECTIVE study ( identifier: NCT01376167) are enrolled in the United Kingdom National Quality Assess...

    journal_title:International journal of laboratory hematology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Roper DR,De la Salle B,Soni V,Fletcher K,Green JA

    更新日期:2017-06-01 00:00:00

  • Evaluation of a new venom-based clotting assay of protein C.

    abstract::Congenital protein C deficiency significantly increases the risk of venous thromboembolism, a serious and potentially lethal condition. Protein C levels can be determined by chromogenic, clotting and antigenic assays, each type of assay has differences in specificity and sensitivity to protein C deficiency. In princip...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Cooper PC,Cooper SM,Goodfellow KJ,Hickey KP,Kitchen S,Makris M

    更新日期:2008-10-01 00:00:00

  • ICSH guidelines for the standardization of bone marrow specimens and reports.

    abstract::The bone marrow examination is an essential investigation for the diagnosis and management of many disorders of the blood and bone marrow. The aspirate and trephine biopsy specimens are complementary and when both are obtained, they provide a comprehensive evaluation of the bone marrow. The final interpretation requir...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,实务指引


    authors: Lee SH,Erber WN,Porwit A,Tomonaga M,Peterson LC,International Council for Standardization In Hematology.

    更新日期:2008-10-01 00:00:00

  • Validation of reflex testing rules and establishment of a new workflow for body fluid cell analysis using a Sysmex XN-550 automatic hematology analyzer.

    abstract:INTRODUCTION:We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS:We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Cho YU,You E,Jang S,Park CJ

    更新日期:2018-06-01 00:00:00

  • How I investigate for bleeding disorders.

    abstract:INTRODUCTION:Laboratory investigations for bleeding disorders are warranted when an individual has a personal and/or family history of bleeding, and/or laboratory findings that suggest the possibility of an inherited or acquired bleeding disorder. METHODS:This review summarizes author's experience with ordering and re...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Hayward CPM

    更新日期:2018-05-01 00:00:00

  • Chronic active Epstein-Barr virus infection: A heterogeneous entity requiring a high index of suspicion for diagnosis.

    abstract::Chronic active Epstein-Barr virus infection of T- and NK-cell type, systemic form, is a rare entity within the spectrum of EBV-driven T- and NK-cell lymphoproliferative disorders. Established diagnostic criteria and a characteristic clinical course help to differentiate it from other closely related EBV-positive neopl...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章,评审


    authors: Ondrejka SL,Hsi ED

    更新日期:2020-06-01 00:00:00

  • Proteasome inhibitor bortezomib overcomes P-gp-mediated multidrug resistance in resistant leukemic cell lines.

    abstract:INTRODUCTION:To study the effect of bortezomib alone or in combination with daunorubicin (DNR) on an mdr1 single-factor drug-resistant leukemia cell line K562/MDR1, a multifactor-resistant cell line K562/A02, a drug-sensitive cell line K562, and primary cells from acute myeloid leukemia patients. METHODS:The cell line...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Zheng B,Zhou R,Gong Y,Yang X,Shan Q

    更新日期:2012-06-01 00:00:00

  • Factor VII assay performance: an analysis of the North American Specialized Coagulation Laboratory Association proficiency testing results.

    abstract::The performance of factor VII (FVII) assays currently used by clinical laboratories was examined in North American Specialized Coagulation Laboratory Association (NASCOLA) proficiency tests. Data from 12 surveys conducted between 2008 and 2010, involving 20 unique specimens plus four repeat-tested specimens, were anal...

    journal_title:International journal of laboratory hematology

    pub_type: 杂志文章


    authors: Zantek ND,Hsu P,Refaai MA,Ledford-Kraemer M,Meijer P,Van Cott EM

    更新日期:2013-06-01 00:00:00